Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis

The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co2+ and Mn2+. Under these reaction conditions, the enzyme displayed Km and kcat values of 0.2...

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Autores:

Machado Sierra, Elwi
Rangel Pereira, Mariana
Carvalho Maester, Thaís
Soares Gomes-Pepe, Elisangela
Rodas Mendoza, Elkin
Macedo Lemos, Elkin

Tipo de recurso:

Fecha de publicación:

2017

Institución:

Universidad Simón Bolívar

Repositorio:

Repositorio Digital USB

Idioma:

eng

OAI Identifier:

oai:bonga.unisimon.edu.co:20.500.12442/1585

Acceso en línea:

http://hdl.handle.net/20.500.12442/1585

Palabra clave:

Mesorhizobium
Aminopeptidase
Microbial Biofilms
Sodium Chloride
Osmosis
Biotechnology
Water-Electrolyte Balance
Organic solvent product

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licencia de Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional