Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses

Recovery in athletes is hampered by soreness and fatigue. Consequently, nonsteroidal antiinflammatory drugs are used as an effective strategy to maintain high performance. However, impact of these drugs on adaptations induced by training remains unknown. This study assessed the effects of diclofenac...

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Autores:: Pillon Barcelos, Rômulo
Diniz Lima, Frederico
Alves Courtes, Aline
Kich da Silva, Ingrid
Vargas, José Eduardo
Freire Royes, Luiz Fernando
Trindade, Cristiano
González-Gallego, Javier
Antunes Soares, Félix Alexandre

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Simón Bolívar

Repositorio:: Repositorio Digital USB

Idioma:: eng

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dc.title.eng.fl_str_mv	Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses
title	Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses
spellingShingle	Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses NSAIDs Physical training Rat Diclofenac Inflammation Oxidative stress Systems biology Systems pharmacology Adaptation
title_short	Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses
title_full	Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses
title_fullStr	Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses
title_full_unstemmed	Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses
title_sort	Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses
dc.creator.fl_str_mv	Pillon Barcelos, Rômulo Diniz Lima, Frederico Alves Courtes, Aline Kich da Silva, Ingrid Vargas, José Eduardo Freire Royes, Luiz Fernando Trindade, Cristiano González-Gallego, Javier Antunes Soares, Félix Alexandre
dc.contributor.author.none.fl_str_mv	Pillon Barcelos, Rômulo Diniz Lima, Frederico Alves Courtes, Aline Kich da Silva, Ingrid Vargas, José Eduardo Freire Royes, Luiz Fernando Trindade, Cristiano González-Gallego, Javier Antunes Soares, Félix Alexandre
dc.subject.eng.fl_str_mv	NSAIDs Physical training Rat Diclofenac Inflammation Oxidative stress Systems biology Systems pharmacology Adaptation
topic	NSAIDs Physical training Rat Diclofenac Inflammation Oxidative stress Systems biology Systems pharmacology Adaptation
description	Recovery in athletes is hampered by soreness and fatigue. Consequently, nonsteroidal antiinflammatory drugs are used as an effective strategy to maintain high performance. However, impact of these drugs on adaptations induced by training remains unknown. This study assessed the effects of diclofenac administration (10 mg/kg/day) on rats subjected to an exhaustive test, after six weeks of swimming training. Over the course of 10 days, three repeated swimming bouts were performed, and diclofenac or saline were administered once a day. Trained animals exhibited higher muscle citrate synthase and lower plasma creatinine kinase activities as compared to sedentary animals, wherein diclofenac had no impact. Training increased time to exhaustion, however, diclofenac blunted this effect. It also impaired the increase in plasma and liver interleukin-6 levels. The trained group exhibited augmented catalase, glutathione peroxidase, and glutathione reductase activities, and a higher ratio of reduced-to-oxidized glutathione in the liver. However, diclofenac treatment blunted all these effects. Systems biology analysis revealed a close relationship between diclofenac and liver catalase. These results confirmed that regular exercise induces inflammation and oxidative stress, which are crucial for tissue adaptations. Altogether, diclofenac treatment might be helpful in preventing pain and inflammation, but its use severely affects performance and tissue adaptation
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-08-04T18:23:40Z
dc.date.available.none.fl_str_mv	2021-08-04T18:23:40Z
dc.date.issued.none.fl_str_mv	2021
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dc.type.spa.spa.fl_str_mv	Artículo científico
dc.identifier.citation.spa.fl_str_mv	Barcelos, R.P.; Lima, F.D.; Courtes, A.A.; Silva, I.K.d.; Vargas, J.E.; Royes, L.F.F.; Trindade, C.; González-Gallego, J.; Soares, F.A.A. Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses. Antioxidants 2021, 10, 1246. https://doi.org/10.3390/ antiox10081246
dc.identifier.issn.none.fl_str_mv	20763921
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12442/8100
dc.identifier.doi.none.fl_str_mv	https://doi.org/10.3390/antiox10081246
dc.identifier.url.none.fl_str_mv	https://www.mdpi.com/2076-3921/10/8/1246
identifier_str_mv	Barcelos, R.P.; Lima, F.D.; Courtes, A.A.; Silva, I.K.d.; Vargas, J.E.; Royes, L.F.F.; Trindade, C.; González-Gallego, J.; Soares, F.A.A. Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses. Antioxidants 2021, 10, 1246. https://doi.org/10.3390/ antiox10081246 20763921
url	https://hdl.handle.net/20.500.12442/8100 https://doi.org/10.3390/antiox10081246 https://www.mdpi.com/2076-3921/10/8/1246
dc.language.iso.eng.fl_str_mv	eng
language	eng
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rights_invalid_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.mimetype.eng.fl_str_mv	pdf
dc.publisher.eng.fl_str_mv	MDPI
dc.source.eng.fl_str_mv	Antioxidants
dc.source.none.fl_str_mv	Vol. 10, No. 8 (2021)
institution	Universidad Simón Bolívar
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spelling	Pillon Barcelos, Rômulof6ea1cf6-5e72-4484-a2e6-e5adaa1a08f7Diniz Lima, Frederico3017fe74-a8ca-493a-94b0-68c81eb4b11cAlves Courtes, Alinebeacae9d-aca6-4b1d-ab78-01655b5b3450Kich da Silva, Ingrid065dfeff-ba3b-47ba-88ae-c5a086768effVargas, José Eduardo416f0def-6df6-4f2d-ad69-3c969af5664dFreire Royes, Luiz Fernando4924f0bd-1c57-4486-864c-02ee37265dc6Trindade, Cristiano30b50065-9290-4b8a-837b-01634d3219d7González-Gallego, Javierb6bb403d-4b1a-4704-9955-6b66c83e4880Antunes Soares, Félix Alexandre7c15e9ce-89b2-4d86-b050-cfda8f953ec22021-08-04T18:23:40Z2021-08-04T18:23:40Z2021Barcelos, R.P.; Lima, F.D.; Courtes, A.A.; Silva, I.K.d.; Vargas, J.E.; Royes, L.F.F.; Trindade, C.; González-Gallego, J.; Soares, F.A.A. Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses. Antioxidants 2021, 10, 1246. https://doi.org/10.3390/ antiox1008124620763921https://hdl.handle.net/20.500.12442/8100https://doi.org/10.3390/antiox10081246https://www.mdpi.com/2076-3921/10/8/1246Recovery in athletes is hampered by soreness and fatigue. Consequently, nonsteroidal antiinflammatory drugs are used as an effective strategy to maintain high performance. However, impact of these drugs on adaptations induced by training remains unknown. This study assessed the effects of diclofenac administration (10 mg/kg/day) on rats subjected to an exhaustive test, after six weeks of swimming training. Over the course of 10 days, three repeated swimming bouts were performed, and diclofenac or saline were administered once a day. Trained animals exhibited higher muscle citrate synthase and lower plasma creatinine kinase activities as compared to sedentary animals, wherein diclofenac had no impact. Training increased time to exhaustion, however, diclofenac blunted this effect. It also impaired the increase in plasma and liver interleukin-6 levels. The trained group exhibited augmented catalase, glutathione peroxidase, and glutathione reductase activities, and a higher ratio of reduced-to-oxidized glutathione in the liver. However, diclofenac treatment blunted all these effects. Systems biology analysis revealed a close relationship between diclofenac and liver catalase. These results confirmed that regular exercise induces inflammation and oxidative stress, which are crucial for tissue adaptations. Altogether, diclofenac treatment might be helpful in preventing pain and inflammation, but its use severely affects performance and tissue adaptationpdfengMDPIAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2AntioxidantsVol. 10, No. 8 (2021)NSAIDsPhysical trainingRatDiclofenacInflammationOxidative stressSystems biologySystems pharmacologyAdaptationDiclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analysesinfo:eu-repo/semantics/articleArtículo científicohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1Warburton, D.E.R.; Nicol, C.W.; Bredin, S.S.D. Health benefits of physical activity: The evidence. CMAJ 2006, 174, 801–809.Ruegsegger, G.N.; Booth, F.W. Health benefits of exercise. Cold Spring Harb. Perspect. 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Sport Med. 2006, 16, 27–33.Ziltener, J.L.; Leal, S.; Fournier, P.E. Non-steroidal anti-inflammatory drugs for athletes: An update. Ann. Phys. Rehabil. Med. 2010, 53, 278–288Barcelos, R.P.; Bresciani, G.; Cuevas, M.J.; Martínez-Flórez, S.; Soares, F.A.A.; González-Gallego, J. Diclofenac pretreatment modulates exercise-induced inflammation in skeletal muscle of rats through the TLR4/NF-κB pathway. Appl. Physiol. Nutr. Metab. 2017, 42, 757–764.Steckling, F.M.; Lima, F.D.; Farinha, J.B.; Rosa, P.C.; Royes, L.F.F.; Cuevas, M.J.; Bresciani, G.; Soares, F.A.; González-Gallego, J.; Barcelos, R.P. Diclofenac attenuates inflammation through TLR4 pathway and improves exercise performance after exhaustive swimming. Scand. J. Med. Sci. Sport. 2020, 30, 264–271Moghetti, P.; Bacchi, E.; Brangani, C.; Donà, S.; Negri, C. Metabolic effects of Exercise. Front. Horm. Res. 2016, 47, 44–57.Murton, A.J.; Greenhaff, P.L. 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Rev. 2006, 12, 6–33.CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://bonga.unisimon.edu.co/bitstreams/3505f572-45f2-4721-8933-f99ead46fdbd/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALPDF.pdfPDF.pdfPDFapplication/pdf2213899https://bonga.unisimon.edu.co/bitstreams/df88dd87-7dfe-47b2-a5d5-627b7efb4c31/download632f7afc854dcb2db54fc2c3e7bbb0b9MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-8381https://bonga.unisimon.edu.co/bitstreams/e2166fc0-6913-4af4-be3e-a1088fdff408/download733bec43a0bf5ade4d97db708e29b185MD53TEXT2021 Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance In Vivo and In Silico Analyses.pdf.txt2021 Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance In Vivo and In Silico Analyses.pdf.txtExtracted texttext/plain60290https://bonga.unisimon.edu.co/bitstreams/a77f54f1-fd00-47c7-982e-16a5cbd328e1/downloadd11e890c3c802a471de9985433426bfaMD54PDF.pdf.txtPDF.pdf.txtExtracted texttext/plain65510https://bonga.unisimon.edu.co/bitstreams/c8ed4ebf-8dba-4d9e-a149-e233ed6d3ddc/downloadaaa3cc8f734331178dbf295514ed3060MD56THUMBNAIL2021 Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance In Vivo and In Silico Analyses.pdf.jpg2021 Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance In Vivo and In Silico Analyses.pdf.jpgGenerated Thumbnailimage/jpeg24130https://bonga.unisimon.edu.co/bitstreams/39ebc4c3-0bb3-4d80-b79b-bbc2b15a7331/download80305067c51985de4d2c7245bec8a83dMD55PDF.pdf.jpgPDF.pdf.jpgGenerated Thumbnailimage/jpeg5935https://bonga.unisimon.edu.co/bitstreams/3e6aa26a-9df6-4bf4-96fd-39129ce85f7d/download94e8d86994cf7137c6d5a83554567581MD5720.500.12442/8100oai:bonga.unisimon.edu.co:20.500.12442/81002024-08-14 21:51:44.34http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internacionalopen.accesshttps://bonga.unisimon.edu.coRepositorio Digital Universidad Simón Bolívarrepositorio.digital@unisimon.edu.coPGEgcmVsPSJsaWNlbnNlIiBocmVmPSJodHRwOi8vY3JlYXRpdmVjb21tb25zLm9yZy9saWNlbnNlcy9ieS1uYy80LjAvIj48aW1nIGFsdD0iTGljZW5jaWEgQ3JlYXRpdmUgQ29tbW9ucyIgc3R5bGU9ImJvcmRlci13aWR0aDowO3dpZHRoOjEwMHB4OyIgc3JjPSJodHRwczovL2kuY3JlYXRpdmVjb21tb25zLm9yZy9sL2J5LW5jLzQuMC84OHgzMS5wbmciIC8+PC9hPjxici8+RXN0YSBvYnJhIGVzdMOhIGJham8gdW5hIDxhIHJlbD0ibGljZW5zZSIgaHJlZj0iaHR0cDovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnktbmMvNC4wLyI+TGljZW5jaWEgQ3JlYXRpdmUgQ29tbW9ucyBBdHJpYnVjacOzbi1Ob0NvbWVyY2lhbCA0LjAgSW50ZXJuYWNpb25hbDwvYT4u

Diclofenac Administration after Physical Training Blunts Adaptations of Peripheral Systems and Leads to Losses in Exercise Performance: In Vivo and In Silico Analyses

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