Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale

The study of xylose reductase (XR) - one of the key enzymes in the production of xylitol - is important in the fermentation process to have maximum efficiency in the bioconversion of xylose to xylitol in lignocellulosic hydrolysate. The aim was to evaluate the effect of agitation rate and dissolved...

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Autores:: Manjarres Pinzón, K

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad del Atlántico

Repositorio:: Repositorio Uniatlantico

Idioma:: eng

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dc.title.spa.fl_str_mv	Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale
title	Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale
spellingShingle	Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale Candida tropicalis xylitol xylose reductase dissolved oxygen non-detoxified hydrolysate
title_short	Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale
title_full	Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale
title_fullStr	Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale
title_full_unstemmed	Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale
title_sort	Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale
dc.creator.fl_str_mv	Manjarres Pinzón, K
dc.contributor.author.none.fl_str_mv	Manjarres Pinzón, K
dc.contributor.other.none.fl_str_mv	Mendoza Meza, D Arias Zabala, M Correa Londoño, G Rodriguez Sandoval, E
dc.subject.keywords.spa.fl_str_mv	Candida tropicalis xylitol xylose reductase dissolved oxygen non-detoxified hydrolysate
topic	Candida tropicalis xylitol xylose reductase dissolved oxygen non-detoxified hydrolysate
description	The study of xylose reductase (XR) - one of the key enzymes in the production of xylitol - is important in the fermentation process to have maximum efficiency in the bioconversion of xylose to xylitol in lignocellulosic hydrolysate. The aim was to evaluate the effect of agitation rate and dissolved oxygen at 7 L bioreactor scale on the production of xylose reductase (XR) from Candida tropicalis during the bioconversion of xylose into xylitol in the non-detoxified oil palm empty fruit bunch (OPEFB) hydrolysate. The highest xylose consumption (95.5%) and the maximum xylitol production (5.46 g.L-1) were presented under 30% dissolved oxygen and 50 rpm. The maximum XR activity (0.646 U mg-1 protein) was obtained after 144 h of fermentation and at the same conditions of dissolved oxygen and agitation rate mentioned above. The oxygen availability influences the XR activity of C. tropicalis and the xylitol production, observing a xylitol yield factor (YP/S) of 0.27 g.g-1 and volumetric productivity (QP) of 0.33 g.L-1 h-1. At lower dissolved oxygen regardless of the agitation conditions evaluated, an increase in xylitol production was evidenced.
publishDate	2021
dc.date.submitted.none.fl_str_mv	2021-01-25
dc.date.accessioned.none.fl_str_mv	2022-11-15T21:15:10Z
dc.date.available.none.fl_str_mv	2022-11-15T21:15:10Z
dc.date.issued.none.fl_str_mv	2022-01-05
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dc.identifier.citation.spa.fl_str_mv	Katherine MANJARRES-PINZÓN, Dary MENDOZA-MEZA and Mario ARIAS-ZABALA et al. Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale. Food Science and Technology. DOI: 10.1590/fst.04221
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dc.identifier.doi.none.fl_str_mv	10.1590/fst.04221
dc.identifier.instname.spa.fl_str_mv	Universidad del Atlántico
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identifier_str_mv	Katherine MANJARRES-PINZÓN, Dary MENDOZA-MEZA and Mario ARIAS-ZABALA et al. Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale. Food Science and Technology. DOI: 10.1590/fst.04221 10.1590/fst.04221 Universidad del Atlántico Repositorio Universidad del Atlántico
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dc.language.iso.spa.fl_str_mv	eng
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dc.publisher.discipline.spa.fl_str_mv	Licenciatura en Ciencias Naturales
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spelling	Manjarres Pinzón, Kae538a5b-8ff8-4f1c-9c30-cb3b385b5032Mendoza Meza, DArias Zabala, MCorrea Londoño, GRodriguez Sandoval, E2022-11-15T21:15:10Z2022-11-15T21:15:10Z2022-01-052021-01-25Katherine MANJARRES-PINZÓN, Dary MENDOZA-MEZA and Mario ARIAS-ZABALA et al. Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale. Food Science and Technology. DOI: 10.1590/fst.04221https://hdl.handle.net/20.500.12834/96010.1590/fst.04221Universidad del AtlánticoRepositorio Universidad del Atlánticohttps://www.scopus.com/record/display.uri?eid=2-s2.0-85126587101&doi=10.1590%2ffst.04221&origin=inward&txGid=003e561f119774e92aa7a4183b3e01f0The study of xylose reductase (XR) - one of the key enzymes in the production of xylitol - is important in the fermentation process to have maximum efficiency in the bioconversion of xylose to xylitol in lignocellulosic hydrolysate. The aim was to evaluate the effect of agitation rate and dissolved oxygen at 7 L bioreactor scale on the production of xylose reductase (XR) from Candida tropicalis during the bioconversion of xylose into xylitol in the non-detoxified oil palm empty fruit bunch (OPEFB) hydrolysate. The highest xylose consumption (95.5%) and the maximum xylitol production (5.46 g.L-1) were presented under 30% dissolved oxygen and 50 rpm. The maximum XR activity (0.646 U mg-1 protein) was obtained after 144 h of fermentation and at the same conditions of dissolved oxygen and agitation rate mentioned above. The oxygen availability influences the XR activity of C. tropicalis and the xylitol production, observing a xylitol yield factor (YP/S) of 0.27 g.g-1 and volumetric productivity (QP) of 0.33 g.L-1 h-1. At lower dissolved oxygen regardless of the agitation conditions evaluated, an increase in xylitol production was evidenced.application/pdfenghttp://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Food Science and TechnologyEffects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scalePúblico generalCandida tropicalisxylitolxylose reductasedissolved oxygennon-detoxified hydrolysateinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BarranquillaLicenciatura en Ciencias NaturalesSede NorteAlbuquerque, T. L., Silva, I. J. Jr., Macedo, G. R., & Rocha, M. V. P. (2014). Biotechnological production of xylitol from lignocellulosic wastes: a review. Process Biochemistry, 49(11), 1779-1789. http:// dx.doi.org/10.1016/j.procbio.2014.07.010.Arruda, P. V., Rodrigues, R. C. L B., Silva, D. D. V. & Felipe, M. G. A. (2011). Evaluation of hexose and pentose in pre-cultivation of Candida guilliermondii on the key enzymes for xylitol production in sugarcane hemicellulosic hydrolysate. Biodegradation, 22(4), 815-822. http://doi: 10.1007/s10532-010-9397-1.Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Analytical Biochemistry, 72(1-2), 248-254. http://dx.doi. org/10.1016/0003-2697(76)90527-3. PMid:942051.Branco, R. F., Santos, J. C., Sarrouh, B. F., Rivaldi, J. D., Pessoa, A. Jr., & Silva, S. S. (2009). Profiles of xylose reductase, xylitol dehydrogenase and xylitol production under different oxygen transfer volumetric coefficient values. 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Applied Biochemistry and Biotechnology Part A Enzyme Engineering and Biotechnology (pp. 449-458). Totowa: Humana Press. http://dx.doi. org/10.1007/978-1-4612-0119-9_37Ferrer, A., Requejo, A., Rodríguez, A., & Jiménez, L. (2013). Influence of temperature, time, liquid/solid ratio and sulfuric acid concentration on the hydrolysis of palm empty fruit bunches. Bioresource Technology, 129, 506-511. http://dx.doi.org/10.1016/j.biortech.2012.10.081. PMid:23266852.Gírio, F. M., Roseiro, J. C., Sá-Machado, P., Duarte-Reis, A. R., & Amaral-Collaço, M. T. (1994). Effect of oxygen transfer rate on levels of key enzymes of xylose metabolism in Debaryomyces hansenii. Enzyme and Microbial Technology, 16(12), 1074-1078. http://dx.doi. org/10.1016/0141-0229(94)90145-7.Gurpilhares, D. B., Hasmann, F. A., Pessoa, A. Jr., & Roberto, I. C. (2009). The behavior of key enzymes of xylose metabolism on the xylitol production by Candida guilliermondii grown in hemicellulosic hydrolysate. 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Xylitol production from non-detoxified and non-sterile lignocellulosic hydrolysate using low-cost industrial media components. 3 Biotech, 7(1), 68. http://doi:10.1007/s13205-017-0700-2. » https://doi.org/http://doi:10.1007/s13205-017-0700-2Zhang, M., Puri, A. K., Wang, Z., Singh, S., & Permaul, K. (2019). A unique xylose reductase from Thermomyces lanuginosus: Effect of lignocellulosic substrates and inhibitors and applicability in lignocellulosic bioconversion. Bioresource Technology, 281, 374-381. http://dx.doi.org/10.1016/j.biortech.2019.02.102 PMid:30831517. » http://dx.doi.org/10.1016/j.biortech.2019.02.102http://purl.org/coar/resource_type/c_2df8fbb1ORIGINALdescarga.pdfdescarga.pdfapplication/pdf947582https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/960/1/descarga.pdfde43d1d1ffc9648c6f02d5df67d87237MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/960/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81306https://repositorio.uniatlantico.edu.co/bitstream/20.500.12834/960/3/license.txt67e239713705720ef0b79c50b2ececcaMD5320.500.12834/960oai:repositorio.uniatlantico.edu.co:20.500.12834/9602022-11-15 16:15:11.454DSpace de la Universidad de Atlánticosysadmin@mail.uniatlantico.edu.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

Effects of agitation rate and dissolved oxygen on xylose reductase activity during xylitol production at bioreactor scale

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