Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral
En este trabajo se realizó el estudio de extractos etanólicos de agrás, obtenidos por extracción asistida por microondas (MAE), en tanto a su contenido de azucar, su contenido polifenólico, su actividad antioxidante y su actividad antitumoral. Para las cuantificaciones de azucares (índice de Brix) y...
- Autores:
-
Monroy Bustos, Isabella
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/59387
- Acceso en línea:
- http://hdl.handle.net/1992/59387
- Palabra clave:
- Vaccinium meridionale
Compuestos polifenólicos
Actividad antioxidante
Actividad antitumoral
Química
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral |
| title |
Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral |
| spellingShingle |
Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral Vaccinium meridionale Compuestos polifenólicos Actividad antioxidante Actividad antitumoral Química |
| title_short |
Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral |
| title_full |
Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral |
| title_fullStr |
Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral |
| title_full_unstemmed |
Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral |
| title_sort |
Evaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoral |
| dc.creator.fl_str_mv |
Monroy Bustos, Isabella |
| dc.contributor.advisor.none.fl_str_mv |
Jiménez Díaz, Elizabeth |
| dc.contributor.author.none.fl_str_mv |
Monroy Bustos, Isabella |
| dc.contributor.jury.none.fl_str_mv |
Miscione, Gian Pietro Zapata Rivera, Jhon Enrique Carazzone, Chiara |
| dc.contributor.researchgroup.es_CO.fl_str_mv |
Grupo de Investigación en Bioquímica Aplicada |
| dc.subject.keyword.none.fl_str_mv |
Vaccinium meridionale Compuestos polifenólicos Actividad antioxidante Actividad antitumoral |
| topic |
Vaccinium meridionale Compuestos polifenólicos Actividad antioxidante Actividad antitumoral Química |
| dc.subject.themes.es_CO.fl_str_mv |
Química |
| description |
En este trabajo se realizó el estudio de extractos etanólicos de agrás, obtenidos por extracción asistida por microondas (MAE), en tanto a su contenido de azucar, su contenido polifenólico, su actividad antioxidante y su actividad antitumoral. Para las cuantificaciones de azucares (índice de Brix) y de contenido polifenólico (Folin-Ciocalteu) se evaluaron tres proporciones de etanol en el medio de extracción: 30, 40 y 50% de etanol. Frente a esto se halló que el contenido de azúcares es mínimo en las tres proporciones, y el contenido polifenólico es máximo en el medio de 50% de etanol. De esta manera los estudios de actividad se realizaron sobre el extracto obtenido con 50% de etanol, dando como resultado actividades antioxidante (TEAC) y antitumoral (MTT) promisorias. |
| publishDate |
2022 |
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2022-07-29T21:25:48Z |
| dc.date.available.none.fl_str_mv |
2022-07-29T21:25:48Z |
| dc.date.issued.none.fl_str_mv |
2022-07-28 |
| dc.type.es_CO.fl_str_mv |
Trabajo de grado - Pregrado |
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info:eu-repo/semantics/bachelorThesis |
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info:eu-repo/semantics/acceptedVersion |
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http://purl.org/redcol/resource_type/TP |
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spa |
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Galvis Rueda, M.; Torres Torres, M. Etnobotánica y Usos de Las Plantas de La Comunidad Rural de Sogamoso, Boyacá, Colombia. Rev. Investig. Agrar. Ambient. 2017, 8 (2), 187-187-206. https://doi.org/10.22490/21456453.2045. Celis, M. E. M.; Franco Tobón, Y. N.; Agudelo, C.; Arango, S. S.; Rojano, B. Andean Berry (Vaccinium Meridionale Swartz). Fruit Veg. Phytochem. Chemestry Hum. Health 2nd Ed Yahia EM Ed 2017, 869-882 Garzón, G. A.; Narváez, C. E.; Riedl, K. M.; Schwartz, S. J. Chemical Composition, Anthocyanins, Non-Anthocyanin Phenolics and Antioxidant Activity of Wild Bilberry ( Vaccinium Meridionale Swartz) from Colombia. Food Chem. 2010, 122 (4), 980-980-986. https://doi.org/10.1016/j.foodchem.2010.03.017 Carmona-Jiménez Y; Palma M; Guillén-Sánchez DA; García-Moreno MV. Study of the Cluster Thinning Grape as a Source of Phenolic Compounds and Evaluation of Its Antioxidant Potential. Biomolecules 2021, 11 (2). https://doi.org/10.3390/biom11020227 Opris, O.; Soran, M.-L.; Lung, I.; Stegarescu, A.; Gutoiu, S.; Podea, R.; Podea, P. Optimization of Extraction Conditions of Polyphenols, Antioxidant Capacity and Sun Protection Factor from Prunus Spinosa Fruits. Application in Sunscreen Formulation. J. Iran. Chem. Soc. 2021, 1-1-12. https://doi.org/10.1007/s13738-021-02217-9 Varo, M. Á.; Martín-Gómez, J.; Mérida, J.; Serratosa, M. P. Bioactive Compounds and Antioxidant Activity of Highbush Blueberry (Vaccinium Corymbosum) Grown in Southern Spain. Eur. Food Res. Technol. 2021, 247 (5), 1199-1199-1208. https://doi.org/10.1007/s00217-021-03701-5 Bastías-Montes, J. M.; Vidal-San Martín, C.; Muñoz-Fariña, O.; Petzold-Maldonado, G.; Quevedo-León, R.; Wang, H.; Yi, Y.; Céspedes-Acuña, C. L. Cryoconcentration Procedure for Aqueous Extracts of Maqui Fruits Prepared by Centrifugation and Filtration from Fruits Harvested in Different Years from the Same Localities. J. Berry Res. 2019, 9 (3), 377-377-394. https://doi.org/10.3233/JBR-180368 Ahmad, M.; Masood, S.; Sultana, S.; Hadda, T. B.; Bader, A.; Zafar, M. Antioxidant and Nutraceutical Value of Wild Medicinal Rubus Berries. Pak. J. Pharm. Sci. 2015, 28 (1), 241-247. Baby, B.; Antony, P.; Vijayan, R. Antioxidant and Anticancer Properties of Berries. Crit. Rev. Food Sci. Nutr. 2018, 58 (15), 2491-2491-2507. https://doi.org/10.1080/10408398.2017.1329198 Bueno, J.; Ramos-Escudero, F.; Sáez-Plaza, P.; Muñoz, A.; José Navas, M.; Asuero, AgustinG. Analysis and Antioxidant Capacity of Anthocyanin Pigments. Part I: General Considerations Concerning Polyphenols and Flavonoids. Crit. Rev. Anal. Chem. 2012, 42 (2), 102-102-125. https://doi.org/10.1080/10408347.2011.632312 Amawi, H.; Ashby, C. R.; Samuel, T.; Peraman, R.; Tiwari, A. K. Polyphenolic Nutrients in Cancer Chemoprevention and Metastasis: Role of the Epithelial-to-Mesenchymal (EMT) Pathway. Nutrients 2017, 9 (8). https://doi.org/10.3390/nu9080911 Gulzar Ahmad Nayik; Amir Gull. Antioxidants in Fruits: Properties and Health Benefits; 2020 Leopoldini, M.; Russo, N.; Toscano, M. The Molecular Basis of Working Mechanism of Natural Polyphenolic Antioxidants. Food Chem. 2011, 125 (2), 288-306. https://doi.org/10.1016/j.foodchem.2010.08.012 Szymanska R; Pospísil P; Kruk J. Plant-Derived Antioxidants in Disease Prevention 2018. Oxidative medicine and cellular longevity, 2018, 2018, 2068370. https://doi.org/10.1155/2018/2068370 Cherubim, D. J.; Martins, C. V.; Fariña, L.; Lucca, R. A. Polyphenols as Natural Antioxidants in Cosmetics Applications. J. Cosmet. Dermatol. 2020, 19 (1), 33-37. https://doi.org/10.1111/jocd.13093 López-Padilla, A.; Martín, D.; Villanueva Bermejo, D.; Jaime, L.; Ruiz-Rodriguez, A.; Restrepo Flórez, C. E.; Rivero Barrios, D. M.; Fornari, T. Vaccinium Meridionale Swartz Extracts and Their Addition in Beef Burgers as Antioxidant Ingredient. J. Sci. Food Agric. 2018, 98 (1), 377-377-383. https://doi.org/10.1002/jsfa.8483 Katsube, N.; Iwashita, K.; Tsushida, T.; Yamaki, K.; Kobori, M. Induction of Apoptosis in Cancer Cells by Bilberry (Vaccinium Myrtillus) and the Anthocyanins. J. Agric. Food Chem. 2003, 51 (1), 68-75. https://doi.org/10.1021/jf025781x Benedict, S. R. A Reagent for the Detection of Reducing Sugars. J. Biol. Chem. 1909, 5 (5), 485-487. https://doi.org/10.1016/S0021-9258(18)91645-5 Mohamed, E. Laboratory Activities to Introduce Carbohydrates Qualitative Analysis to College Students. World J. Chem. Educ. 2018, 6 (2), 82-86. https://doi.org/10.12691/WJCE-6-2-1 Ainsworth, E. A.; Gillespie, K. M. Estimation of Total Phenolic Content and Other Oxidation Substrates in Plant Tissues Using Folin-Ciocalteu Reagent. Nat. Protoc. 2007, 2 (4), 875-875-877. https://doi.org/10.1038/nprot.2007.102 Pueyo, I. U.; Calvo, M. I. Assay Conditions and Validation of a New UV Spectrophotometric Method Using Microplates for the Determination of Polyphenol Content. Fitoterapia 2009, 80 (8), 465-465-467. https://doi.org/10.1016/j.fitote.2009.06.008 Nenadis, N.; Wang, L.-F.; Tsimidou, M.; Zhang, H.-Y. Estimation of Scavenging Activity of Phenolic Compounds Using the ABTS + Assay. J. Agric. Food Chem. 2004, 52 (15), 4669-4674. https://doi.org/10.1021/jf0400056 Melo Torres, C. P. Rational Approach to Evaluate Interaction Effect on Combination Therapy for Cancer Treatment with Doxorubicin and Non- Traditional Chemotherapeutic Drugs (Metformin, Losartan, Taurine and Salicylic Acid ). 2021 Queiroz, C.; Mendes Lopes, M. L.; Fialho, E.; Valente-Mesquita, V. L. Polyphenol Oxidase: Characteristics and Mechanisms of Browning Control. Food Rev. Int. 2008, 24 (4), 361-375. https://doi.org/10.1080/87559120802089332 Riahi, S.; Moghaddam, A. B.; Ganjali, M. R.; Norouzi, P. Determination Of The Oxidation Potentials Of Pyrogallol And Some Of Its Derivatives: Theory And Experiment. J. Theor. Comput. Chem. 2007, 06 (02), 331-340. Https://Doi.Org/10.1142/S0219633607003015 Alberty, R. A. Standard Apparent Reduction Potentials of Biochemical Half Reactions and Thermodynamic Data on the Species Involved. Biophys. Chem. 2004, 111 (2), 115-122. https://doi.org/10.1016/j.bpc.2004.05.003 Bouchard, A.; Hofland, G. W.; Witkamp, G.-J. Properties of Sugar, Polyol, and Polysaccharide Water-Ethanol Solutions. J. Chem. Eng. Data 2007, 52 (5), 1838-1842. https://doi.org/10.1021/je700190m Cerezo, A. B.; Catunescu, G. M.; González, M. M.; Hornedo-Ortega, R.; Pop, C. R.; Rusu, C. C.; Chirila, F.; Rotar, A. M.; Garcia-Parrilla, M. C.; Troncoso, A. M. Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective against Urinary Tract Bacteria. Antioxidants 2020, 9 (6). https://doi.org/10.3390/antiox9060478 Margraf, T.; Karnopp, A. R.; Rosso, N. D.; Granato, D. Comparison between Folin-Ciocalteu and Prussian Blue Assays to Estimate The Total Phenolic Content of Juices and Teas Using 96-Well Microplates. J. FOOD Sci. 2015, 80 (11), C2397. https://doi.org/10.1111/1750-3841.13077 Cháirez-Ramírez, M. H.; de la Cruz-López, K. G.; García-Carrancá, A. Polyphenols as Antitumor Agents Targeting Key Players in Cancer-Driving Signaling Pathways. Front. Pharmacol. 2021, 12 Çelik, S. E.; Özyürek, M.; Güçlü, K.; Apak, R. Solvent Effects on the Antioxidant Capacity of Lipophilic and Hydrophilic Antioxidants Measured by CUPRAC, ABTS/Persulphate and FRAP Methods. Talanta 2010, 81 (4), 1300-1309. https://doi.org/10.1016/j.talanta.2010.02.025 Rai, Y.; Pathak, R.; Kumari, N.; Sah, D. K.; Pandey, S.; Kalra, N.; Soni, R.; Dwarakanath, B. S.; Bhatt, A. N. Mitochondrial Biogenesis and Metabolic Hyperactivation Limits the Application of MTT Assay in the Estimation of Radiation Induced Growth Inhibition. Sci. Rep. 2018, 8 (1), 1531-1531. https://doi.org/10.1038/s41598-018-19930-w Macdougall, J. Analysis of Dose-Response Studies-Emax Model. In Dose Finding in Drug Development; Ting, N., Ed.; Springer New York: New York, NY, 2006; pp 127-145. https://doi.org/10.1007/0-387-33706-7_9 Gubler, H.; Schopfer, U.; Jacoby, E. Theoretical and Experimental Relationships between Percent Inhibition and IC50 Data Observed in High-Throughput Screening. J. Biomol. Screen. 2013, 18 (1), 1-13. https://doi.org/10.1177/1087057112455219 Maldonado-Celis, M. E.; Arango-Varela, S. S.; Rojano, B. A. Free Radical Scavenging Capacity and Cytotoxic and Antiproliferative Effects of Vaccinium Meridionale Sw. Agains Colon Cancer Cell Lines. Rev. Cuba. Plantas Med. 2014, 19, 172-184 Alara, O. R.; Abdurahman, N. H.; Ukaegbu, C. I. Extraction of Phenolic Compounds: A Review. Curr. Res. Food Sci. 2021, 4, 200-214. https://doi.org/10.1016/j.crfs.2021.03.011 Kaur, N.; Aggarwal, P.; Kumar, V.; Kaur, S. Influence of Different Extraction Techniques on the Extraction of Phytochemicals and Antioxidant Activities from Syzygium Cumini (Jamun) Pomace Using Taguchi Orthogonal Array Design: A Qualitative and Quantitative Approach. Biomass Convers. Biorefinery Process. Biog. Mater. Energy Chem. 2022, 1-1-13. https://doi.org/10.1007/s13399-022-02826-1 Agudelo, C. D.; Luzardo-Ocampo, I.; Hernández-Arriaga, A. M.; Rendón, J. C.; Campos-Vega, R.; Maldonado-Celis, M. E. Fermented Non-Digestible Fraction of Andean Berry (Vaccinium Meridionale Swartz) Juice Induces Apoptosis in Colon Adenocarcinoma Cells. Prev. Nutr. Food Sci. 2020, 25 (3), 272-279. https://doi.org/10.3746/pnf.2020.25.3.272 Zapata Vahos, I. C.; Ochoa Agudelo, S.; Alzate Arbeláez, A. F.; Zapata Zapata, A. D.; Rojano, B. A. Vinegar Of Andean Berries (Vaccinium Meridionale Sw): Antioxidant And Antiproliferative Activity In Colon Cancer Cells Sw480. Vitae 2019, 26 Shi, D.; Xie, F.; Zhai, C.; Stern, J. S.; Liu, Y.; Liu, S. The Role of Cellular Oxidative Stress in Regulating Glycolysis Energy Metabolism in Hepatoma Cells. Mol. Cancer 2009, 8 (1), 32. https://doi.org/10.1186/1476-4598-8-32 Alhosin, M.; León-González, A. J.; Dandache, I.; Lelay, A.; Rashid, S. K.; Kevers, C.; Pincemail, J.; Fornecker, L.-M.; Mauvieux, L.; Herbrecht, R.; Schini-Kerth, V. B. Bilberry Extract (Antho 50) Selectively Induces Redox-Sensitive Caspase 3-Related Apoptosis in Chronic Lymphocytic Leukemia Cells by Targeting the Bcl-2/Bad Pathway. Sci. Rep. 2015, 5 (1), 8996. https://doi.org/10.1038/srep08996 Somasagara, R. R.; Hegde, M.; Chiruvella, K. K.; Musini, A.; Choudhary, B.; Raghavan, S. C. Extracts of Strawberry Fruits Induce Intrinsic Pathway of Apoptosis in Breast Cancer Cells and Inhibits Tumor Progression in Mice. PLOS ONE 2012, 7 (10), e47021. https://doi.org/10.1371/journal.pone.0047021 Wlodkowic, D.; Skommer, J.; Darzynkiewicz, Z. Flow Cytometry-Based Apoptosis Detection. In Apoptosis: Methods and Protocols, Second Edition; Erhardt, P., Toth, A., Eds.; Humana Press: Totowa, NJ, 2009; pp 19-32. https://doi.org/10.1007/978-1-60327-017-5_2 Mileo, A. M.; Nisticò, P.; Miccadei, S. Polyphenols: Immunomodulatory and Therapeutic Implication in Colorectal Cancer. Front. Immunol. 2019, 10 Gaviria, M. F. Optimización de La Extracción Asistida Por Microondas En Mezclas Etanólicas de Vaccinium Meridionale y El Estudio de Su Actividad Antioxidante y Antimicrobiana., 2019 Melo Torres, C. P. Implementación Del Ensayo MTT (Bromuro de 3-(3,4 -Dimetiltiazol-2-Il)-2,5- Difeniltetrazolio) En Líneas Celulares Tumorales y Determinación de La Viabilidad Celular de Análogos de Ácidos Nucleico.,2017 |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Jiménez Díaz, Elizabethvirtual::11129-1Monroy Bustos, Isabellaa300d8a4-218a-44cd-adf6-b1664d20ced9600Miscione, Gian PietroZapata Rivera, Jhon EnriqueCarazzone, ChiaraGrupo de Investigación en Bioquímica Aplicada2022-07-29T21:25:48Z2022-07-29T21:25:48Z2022-07-28http://hdl.handle.net/1992/59387instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/En este trabajo se realizó el estudio de extractos etanólicos de agrás, obtenidos por extracción asistida por microondas (MAE), en tanto a su contenido de azucar, su contenido polifenólico, su actividad antioxidante y su actividad antitumoral. Para las cuantificaciones de azucares (índice de Brix) y de contenido polifenólico (Folin-Ciocalteu) se evaluaron tres proporciones de etanol en el medio de extracción: 30, 40 y 50% de etanol. Frente a esto se halló que el contenido de azúcares es mínimo en las tres proporciones, y el contenido polifenólico es máximo en el medio de 50% de etanol. De esta manera los estudios de actividad se realizaron sobre el extracto obtenido con 50% de etanol, dando como resultado actividades antioxidante (TEAC) y antitumoral (MTT) promisorias.QuímicoPregrado34 páginasapplication/pdfspaUniversidad de los AndesQuímicaFacultad de CienciasDepartamento de QuímicaEvaluación del contenido de azúcares y polifenoles del extracto de Vaccinium meridionale en diferentes medios etanólicos para el estudio de su actividad antioxidante y antitumoralTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPVaccinium meridionaleCompuestos polifenólicosActividad antioxidanteActividad antitumoralQuímicaGalvis Rueda, M.; Torres Torres, M. Etnobotánica y Usos de Las Plantas de La Comunidad Rural de Sogamoso, Boyacá, Colombia. Rev. Investig. Agrar. Ambient. 2017, 8 (2), 187-187-206. https://doi.org/10.22490/21456453.2045.Celis, M. E. 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Implementación Del Ensayo MTT (Bromuro de 3-(3,4 -Dimetiltiazol-2-Il)-2,5- Difeniltetrazolio) En Líneas Celulares Tumorales y Determinación de La Viabilidad Celular de Análogos de Ácidos Nucleico.,2017201713863Publicationhttps://scholar.google.es/citations?user=jIgIKoAAAAAJvirtual::11129-10000-0001-7798-2194virtual::11129-1525dcdb4-7349-4e54-bf7d-6dcb9c7cad8dvirtual::11129-1525dcdb4-7349-4e54-bf7d-6dcb9c7cad8dvirtual::11129-1ORIGINALEvaluacion del contenido de azucares y polifenoles del extracto de Vaccinium meridionale.pdfEvaluacion del contenido de azucares y polifenoles del extracto de Vaccinium meridionale.pdfDocumento completoapplication/pdf661194https://repositorio.uniandes.edu.co/bitstreams/afebd447-82ec-4c68-9364-671fb1576363/downloadeb4671c786db4e779fa1957bad56616eMD52Formulario autorización tesis - Isabella.pdfFormulario autorización tesis - Isabella.pdfHIDEapplication/pdf195017https://repositorio.uniandes.edu.co/bitstreams/c8baa63d-59c1-4601-b3af-18f715c98e57/download88a51fbc580e95dc2797e6d1e55196ebMD54THUMBNAILEvaluacion del contenido de azucares y polifenoles del extracto de Vaccinium meridionale.pdf.jpgEvaluacion del contenido de azucares y polifenoles del extracto de Vaccinium meridionale.pdf.jpgIM Thumbnailimage/jpeg9187https://repositorio.uniandes.edu.co/bitstreams/9904e9f6-0b91-4685-a076-c33cb533494a/downloadd56a416c1ff7fe653889a9c145b86f7cMD56Formulario autorización tesis - Isabella.pdf.jpgFormulario autorización tesis - Isabella.pdf.jpgIM Thumbnailimage/jpeg17092https://repositorio.uniandes.edu.co/bitstreams/d741c8ff-a81a-4dab-9dbf-b20e3c7d547e/downloadd7fce16d7e39004c49a3b0a7e0d1df27MD58LICENSElicense.txtlicense.txttext/plain; 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