Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos
Los carotenoides son pigmentos de estructura polienica con importantes funciones fotoprotectoras en las plantas, así como múltiples beneficios para la prevención y el tratamiento de enfermedades por sus propiedades antioxidantes, antinflamatorias y anticancerígenas en humanos. Por ello, en este trab...
- Autores:
-
Marin Mena, Karla Paola
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad de los Andes
- Repositorio:
- Séneca: repositorio Uniandes
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uniandes.edu.co:1992/58727
- Acceso en línea:
- http://hdl.handle.net/1992/58727
- Palabra clave:
- Carotenoides
Ají
Química
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos |
| title |
Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos |
| spellingShingle |
Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos Carotenoides Ají Química |
| title_short |
Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos |
| title_full |
Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos |
| title_fullStr |
Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos |
| title_full_unstemmed |
Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos |
| title_sort |
Identificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianos |
| dc.creator.fl_str_mv |
Marin Mena, Karla Paola |
| dc.contributor.advisor.none.fl_str_mv |
Carazzone, Chiara |
| dc.contributor.author.none.fl_str_mv |
Marin Mena, Karla Paola |
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Zapata Rivera, Jhon Enrique Miscione, Gian Pietro Hurtado Belalcazar, John Jady |
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LATNAP |
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Carotenoides Ají |
| topic |
Carotenoides Ají Química |
| dc.subject.themes.es_CO.fl_str_mv |
Química |
| description |
Los carotenoides son pigmentos de estructura polienica con importantes funciones fotoprotectoras en las plantas, así como múltiples beneficios para la prevención y el tratamiento de enfermedades por sus propiedades antioxidantes, antinflamatorias y anticancerígenas en humanos. Por ello, en este trabajo de investigación se realizó la identificación putativa de carotenoides y derivados de clorofilas presentes ocho especies de ajíes que se consumen en diferentes regiones de Colombia, la cual se realizó mediante el análisis de sus espectros de absorción en la región UV-vis y los patrones de fragmentación obtenidos por espectrometría de masas. Empleando cromatografía líquida de alta resolución con detector de arreglo de diodos acoplada a un espectrómetro de masas en tándem (HPLC-DAD-APCI-MS/MS) se logró la identificación de 57 carotenoides libres y 72 carotenoides esterificados en las muestras analizadas. La separación cromatográfica se realizó empleando una columna C30 que posibilito diferenciar varios de los isómeros geométricos presentes en las muestras, como es el caso del 15-cis-, 13-cis-, 9-cis- y all trans-B-carotene, además de los isómeros 13-cis-B-Cryptoxanthin y all-trans-B-Cryptoxanthin. Adicionalmente, se realizó la identificación de otros carotenoides de tipo epóxidos como lo son: XX y YY, así como derivados de clorofilas. En conclusión, la metodología empleada fue idónea para la detallada caracterización de los carotenoides y derivados de clorofilas presentes en las ocho especies de ajíes. |
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2022 |
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2022-07-11T20:32:23Z |
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2022-07-11T20:32:23Z |
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2022-06-15 |
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Trabajo de grado - Pregrado |
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O.; Olanrewaju, I. O.; Walton, J. C.; Aldabbagh, F. Molecules Free Radical Mediated Oxidative Degradation of Carotenes and Xanthophylls. https://doi.org/10.3390/molecules25051038. Carpentier, S.; Knaus, M.; Suh, M. Associations between Lutein, Zeaxanthin, and Age-Related Macular Degeneration: An Overview. http://dx.doi.org/10.1080/10408390802066979 2009, 49 (4), 313-326. https://doi.org/10.1080/10408390802066979. de Rosso, V. v.; Mercadante, A. Z. Identification and Quantification of Carotenoids, by HPLC-PDA-MS/MS, from Amazonian Fruits. Journal of Agricultural and Food Chemistry 2007, 55 (13), 5062-5072. https://doi.org/10.1021/jf0705421. Demmigadams, B.; Gilmore, A. M.; Adams Up, W. W. In Vivo Functions of Carotenoids in Higher Plants; In Vivo Functions of Carotenoids in Higher Plants. https://doi.org/10.1096/fasebj.10.4.8647339. van Breemen, R. B.; Dong, L.; Pajkovic, N. D. Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry of Carotenoids. International Journal of Mass Spectrometry 2012, 312, 163-172. https://doi.org/10.1016/j.ijms.2011.07.030. Perez-Fons, L.; Steiger, S.; Khaneja, R.; Bramley, P. M.; Cutting, S. M.; Sandmann, G.; Fraser, P. D. Identification and the Developmental Formation of Carotenoid Pigments in the Yellow/Orange Bacillus Spore-Formers. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 2011, 1811 (3), 177-185. https://doi.org/10.1016/j.bbalip.2010.12.009. Crupi, P.; Milella, R. A.; Antonacci, D. Simultaneous HPLC-DAD-MS (ESI+) Determination of Structural and Geometrical Isomers of Carotenoids in Mature Grapes. Journal of Mass Spectrometry 2010, 45 (9), 971-980. https://doi.org/10.1002/jms.1794. Girón, J. M.; Armando Martínez, J.; Hurtado, L. G.; David Cuaran, J.; Ocampo, Y. A. PIGMENTOS VEGETALES Y COMPUESTOS NATURALES APLICADOS EN PRODUCTOS CÁRNICOS COMO COLORANTES Y/O ANTIOXIDANTES: REVISIÓN VEGETABLE PIGMENTS AND NATURAL COMPOUNDS APPLIED AS COLORANT AND ANTIOXIDANTS IN MEAT PRODUCTS: REVIEW. PIGMENTOS VEGETAIS E COMPOSTOS NATURAIS APLICADOS EM PRODUTOS CÁRNICOS COMO COLORANTES E/OU ANTIOXIDANTES: REVISÃO. |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Carazzone, Chiara035262c0-46c2-48a6-969d-854978373cd1600Marin Mena, Karla Paolabd0fbc46-bf5b-4647-af02-29fe86baf0be600Zapata Rivera, Jhon EnriqueMiscione, Gian PietroHurtado Belalcazar, John JadyLATNAP2022-07-11T20:32:23Z2022-07-11T20:32:23Z2022-06-15http://hdl.handle.net/1992/58727instname:Universidad de los Andesreponame:Repositorio Institucional Sénecarepourl:https://repositorio.uniandes.edu.co/Los carotenoides son pigmentos de estructura polienica con importantes funciones fotoprotectoras en las plantas, así como múltiples beneficios para la prevención y el tratamiento de enfermedades por sus propiedades antioxidantes, antinflamatorias y anticancerígenas en humanos. Por ello, en este trabajo de investigación se realizó la identificación putativa de carotenoides y derivados de clorofilas presentes ocho especies de ajíes que se consumen en diferentes regiones de Colombia, la cual se realizó mediante el análisis de sus espectros de absorción en la región UV-vis y los patrones de fragmentación obtenidos por espectrometría de masas. Empleando cromatografía líquida de alta resolución con detector de arreglo de diodos acoplada a un espectrómetro de masas en tándem (HPLC-DAD-APCI-MS/MS) se logró la identificación de 57 carotenoides libres y 72 carotenoides esterificados en las muestras analizadas. La separación cromatográfica se realizó empleando una columna C30 que posibilito diferenciar varios de los isómeros geométricos presentes en las muestras, como es el caso del 15-cis-, 13-cis-, 9-cis- y all trans-B-carotene, además de los isómeros 13-cis-B-Cryptoxanthin y all-trans-B-Cryptoxanthin. Adicionalmente, se realizó la identificación de otros carotenoides de tipo epóxidos como lo son: XX y YY, así como derivados de clorofilas. En conclusión, la metodología empleada fue idónea para la detallada caracterización de los carotenoides y derivados de clorofilas presentes en las ocho especies de ajíes.QuímicoPregrado26 páginasapplication/pdfspaUniversidad de los AndesQuímicaFacultad de CienciasDepartamento de QuímicaIdentificación de carotenoides mediante HPLC-MS/MS en ocho variedades de ajíes colombianosTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttp://purl.org/redcol/resource_type/TPCarotenoidesAjíQuímicaSaha, S.; Walia, S.; Sharma, K.; Banerjee, K. Suitability of Stationary Phase for LC Analysis of Biomolecules. Crit Rev Food Sci Nutr 2020, 60 (17), 2856-2873. https://doi.org/10.1080/10408398.2019.1665494.Mariutti, L. R. B.; Mercadante, A. Z. Carotenoid Esters Analysis and Occurrence: What Do We Know so Far? Archives of Biochemistry and Biophysics 2018, 648, 36-43. https://doi.org/10.1016/J.ABB.2018.04.005.Wang, C.; Zhao, S.; Shao, X.; Park, J. bin; Jeong, S. H.; Park, H. J.; Kwak, W. J.; Wei, G.; Kim, S. W. Challenges and Tackles in Metabolic Engineering for Microbial Production of Carotenoids. Microbial Cell Factories 2019, 18 (1), 1-8. https://doi.org/10.1186/S12934-019-1105-1/FIGURES/2.Kurz, C.; Carle, R.; Schieber, A. HPLC-DAD-MSn Characterisation of Carotenoids from Apricots and Pumpkins for the Evaluation of Fruit Product Authenticity. Food Chemistry 2008, 110 (2), 522-530. https://doi.org/10.1016/j.foodchem.2008.02.022.van Breemen, R. B.; Canjura, F. L.; Schwartzg, S. J. Identification of Chlorophyll Derivatives by Mass Spectrometry+; 1991; Vol. 39.Mercadante, A. Z.; Rodrigues, D. B.; Petry, F. C.; Mariutti, L. R. B. Carotenoid Esters in Foods - A Review and Practical Directions on Analysis and Occurrence. 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LWT 2021, 146. https://doi.org/10.1016/j.lwt.2021.111654.Schex, R.; Lieb, V. M.; Jiménez, V. M.; Esquivel, P.; Schweiggert, R. M.; Carle, R.; Steingass, C. B. HPLC-DAD-APCI/ESI-MSn Analysis of Carotenoids and [alfa]-Tocopherol in Costa Rican Acrocomia Aculeata Fruits of Varying Maturity Stages. Food Research International 2018, 105, 645-653. https://doi.org/10.1016/j.foodres.2017.11.041.Zepka, L. Q.; Mercadante, A. Z. Degradation Compounds of Carotenoids Formed during Heating of a Simulated Cashew Apple Juice. Food Chemistry 2009, 117 (1), 28-34. https://doi.org/10.1016/j.foodchem.2009.03.071.Mussagy, C. U.; Winterburn, J.; Santos-Ebinuma, V. C.; Pereira, J. F. B. Production and Extraction of Carotenoids Produced by Microorganisms. Applied Microbiology and Biotechnology 2019, 103 (3), 1095-1114. https://doi.org/10.1007/s00253-018-9557-5.Marín, A.; Ferreres, F.; Tomás-Barberán, F. A.; Gil, M. I. 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PIGMENTOS VEGETALES Y COMPUESTOS NATURALES APLICADOS EN PRODUCTOS CÁRNICOS COMO COLORANTES Y/O ANTIOXIDANTES: REVISIÓN VEGETABLE PIGMENTS AND NATURAL COMPOUNDS APPLIED AS COLORANT AND ANTIOXIDANTS IN MEAT PRODUCTS: REVIEW. 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