Design and validation of a quantitative polymerase chain reaction test for the identification and quantification of uncultivable bacteria associated with periodontitis

Objective: This study aimed to standardize a quantitative polymerase chain reaction (qPCR)-based test to identify and quantify the uncultivable bacteria associated with periodontitis. Methods: The standardization of qPCR, the curves for the quantification of Eubacterium saphenum, Eubacterium brachy,...

Full description

Autores:

Castillo, Yormaris
Delgadillo, Nathaly Andrea
Neuta, Yineth
Iniesta, Margarita
Sanz, Mariano
Herrera, David
Pianeta, Roquelina
Lafaurie, Gloria Inés
Castillo, Diana Marcela

Tipo de recurso:

Article of journal

Fecha de publicación:

2023

Institución:

Universidad El Bosque

Repositorio:

Repositorio U. El Bosque

Idioma:

eng

OAI Identifier:

oai:repositorio.unbosque.edu.co:20.500.12495/11082

Acceso en línea:

http://hdl.handle.net/20.500.12495/11082
https://doi.org/10.1016/j.archoralbio.2023.105758

Palabra clave:

Eficacia de la PCR
QPCR
Número de copias
Bacterias orales
Periodontitis
PCR efficiency
QPCR
Copy number
Oral bacteria
Periodontitis

Rights

openAccess

License

Attribution-NonCommercial-NoDerivatives 4.0 Internacional