A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains

Humanized mice are an invaluable tool for investigating human diseases such as cancer, infectious diseases, and graft-versus-host disease (GvHD). However, it is crucial to understand the strengths and limitations of humanized mice and select the most appropriate model. In this study, we describe the...

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Autores:
Gutiérrez-Barbosa, Hernando
Medina-Moreno, Sandra
Perdomo-Celis, Federico
Davis, Harry
Coronel-Ruiz, Carolina
Zapata, Juan C.
Chua, Joel V.
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/11103
Acceso en línea:
http://hdl.handle.net/20.500.12495/11103
https://doi.org/10.3390/microorganisms11061548
Palabra clave:
Humanización
Modelo de ratón humanizado
Xenoinjerto
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Linfoide
Mieloide
Humanization
Humanized mouse model
Xenograft
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Lymphoid
Myeloid
Rights
openAccess
License
Atribución 4.0 Internacional
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dc.title.spa.fl_str_mv A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains
title A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains
spellingShingle A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains
Humanización
Modelo de ratón humanizado
Xenoinjerto
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Linfoide
Mieloide
Humanization
Humanized mouse model
Xenograft
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Lymphoid
Myeloid
title_short A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains
title_full A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains
title_fullStr A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains
title_full_unstemmed A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains
title_sort A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains
dc.creator.fl_str_mv Gutiérrez-Barbosa, Hernando
Medina-Moreno, Sandra
Perdomo-Celis, Federico
Davis, Harry
Coronel-Ruiz, Carolina
Zapata, Juan C.
Chua, Joel V.
dc.contributor.author.none.fl_str_mv Gutiérrez-Barbosa, Hernando
Medina-Moreno, Sandra
Perdomo-Celis, Federico
Davis, Harry
Coronel-Ruiz, Carolina
Zapata, Juan C.
Chua, Joel V.
dc.subject.spa.fl_str_mv Humanización
Modelo de ratón humanizado
Xenoinjerto
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Linfoide
Mieloide
topic Humanización
Modelo de ratón humanizado
Xenoinjerto
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Linfoide
Mieloide
Humanization
Humanized mouse model
Xenograft
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Lymphoid
Myeloid
dc.subject.keywords.spa.fl_str_mv Humanization
Humanized mouse model
Xenograft
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Lymphoid
Myeloid
description Humanized mice are an invaluable tool for investigating human diseases such as cancer, infectious diseases, and graft-versus-host disease (GvHD). However, it is crucial to understand the strengths and limitations of humanized mice and select the most appropriate model. In this study, we describe the development of the human lymphoid and myeloid lineages using a flow cytometric analysis in four humanized mouse models derived from NOD mice xenotransplanted with CD34+ fetal cord blood from a single donor. Our results showed that all murine strains sustained human immune cells within a proinflammatory environment induced by GvHD. However, the Hu-SGM3 model consistently generated higher numbers of human T cells, monocytes, dendritic cells, mast cells, and megakaryocytes, and a low number of circulating platelets showing an activated profile when compared with the other murine strains. The hu-NOG-EXL model had a similar cell development profile but a higher number of circulating platelets with an inactivated state, and the hu-NSG and hu-NCG developed low frequencies of immune cells compared with the other models. Interestingly, only the hu-SGM3 and hu-EXL models developed mast cells. In conclusion, our findings highlight the importance of selecting the appropriate humanized mouse model for specific research questions, considering the strengths and limitations of each model and the immune cell populations of interest. © 2023 by the authors.
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-07-19T21:09:50Z
dc.date.available.none.fl_str_mv 2023-07-19T21:09:50Z
dc.date.issued.none.fl_str_mv 2023
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dc.identifier.doi.none.fl_str_mv https://doi.org/10.3390/microorganisms11061548
dc.identifier.instname.spa.fl_str_mv instname:Universidad El Bosque
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad El Bosque
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url http://hdl.handle.net/20.500.12495/11103
https://doi.org/10.3390/microorganisms11061548
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartofseries.spa.fl_str_mv Microorganisms, 2076-2607, 11 (6), 2023, 1 - 19
dc.relation.uri.none.fl_str_mv https://www.mdpi.com/2076-2607/11/6/1548
dc.rights.*.fl_str_mv Atribución 4.0 Internacional
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dc.rights.local.spa.fl_str_mv Acceso abierto
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Acceso abierto
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eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.publisher.journal.spa.fl_str_mv Microorganisms
institution Universidad El Bosque
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spelling Gutiérrez-Barbosa, HernandoMedina-Moreno, SandraPerdomo-Celis, FedericoDavis, HarryCoronel-Ruiz, CarolinaZapata, Juan C.Chua, Joel V.2023-07-19T21:09:50Z2023-07-19T21:09:50Z20232076-2607http://hdl.handle.net/20.500.12495/11103https://doi.org/10.3390/microorganisms11061548instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengMultidisciplinary Digital Publishing InstituteMicroorganismsMicroorganisms, 2076-2607, 11 (6), 2023, 1 - 19https://www.mdpi.com/2076-2607/11/6/1548Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Acceso abiertohttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessHumanizaciónModelo de ratón humanizadoXenoinjertoCD34NSGNCGNOG-EXLNSG-SGM3LinfoideMieloideHumanizationHumanized mouse modelXenograftCD34NSGNCGNOG-EXLNSG-SGM3LymphoidMyeloidA Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse StrainsArtículo de revistainfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85Humanized mice are an invaluable tool for investigating human diseases such as cancer, infectious diseases, and graft-versus-host disease (GvHD). However, it is crucial to understand the strengths and limitations of humanized mice and select the most appropriate model. In this study, we describe the development of the human lymphoid and myeloid lineages using a flow cytometric analysis in four humanized mouse models derived from NOD mice xenotransplanted with CD34+ fetal cord blood from a single donor. Our results showed that all murine strains sustained human immune cells within a proinflammatory environment induced by GvHD. However, the Hu-SGM3 model consistently generated higher numbers of human T cells, monocytes, dendritic cells, mast cells, and megakaryocytes, and a low number of circulating platelets showing an activated profile when compared with the other murine strains. The hu-NOG-EXL model had a similar cell development profile but a higher number of circulating platelets with an inactivated state, and the hu-NSG and hu-NCG developed low frequencies of immune cells compared with the other models. Interestingly, only the hu-SGM3 and hu-EXL models developed mast cells. 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