A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains

Humanized mice are an invaluable tool for investigating human diseases such as cancer, infectious diseases, and graft-versus-host disease (GvHD). However, it is crucial to understand the strengths and limitations of humanized mice and select the most appropriate model. In this study, we describe the...

Full description

Autores:
Gutiérrez-Barbosa, Hernando
Medina-Moreno, Sandra
Perdomo-Celis, Federico
Davis, Harry
Coronel-Ruiz, Carolina
Zapata, Juan C.
Chua, Joel V.
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad El Bosque
Repositorio:
Repositorio U. El Bosque
Idioma:
eng
OAI Identifier:
oai:repositorio.unbosque.edu.co:20.500.12495/11103
Acceso en línea:
http://hdl.handle.net/20.500.12495/11103
https://doi.org/10.3390/microorganisms11061548
Palabra clave:
Humanización
Modelo de ratón humanizado
Xenoinjerto
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Linfoide
Mieloide
Humanization
Humanized mouse model
Xenograft
CD34
NSG
NCG
NOG-EXL
NSG-SGM3
Lymphoid
Myeloid
Rights
openAccess
License
Atribución 4.0 Internacional
Description
Summary:Humanized mice are an invaluable tool for investigating human diseases such as cancer, infectious diseases, and graft-versus-host disease (GvHD). However, it is crucial to understand the strengths and limitations of humanized mice and select the most appropriate model. In this study, we describe the development of the human lymphoid and myeloid lineages using a flow cytometric analysis in four humanized mouse models derived from NOD mice xenotransplanted with CD34+ fetal cord blood from a single donor. Our results showed that all murine strains sustained human immune cells within a proinflammatory environment induced by GvHD. However, the Hu-SGM3 model consistently generated higher numbers of human T cells, monocytes, dendritic cells, mast cells, and megakaryocytes, and a low number of circulating platelets showing an activated profile when compared with the other murine strains. The hu-NOG-EXL model had a similar cell development profile but a higher number of circulating platelets with an inactivated state, and the hu-NSG and hu-NCG developed low frequencies of immune cells compared with the other models. Interestingly, only the hu-SGM3 and hu-EXL models developed mast cells. In conclusion, our findings highlight the importance of selecting the appropriate humanized mouse model for specific research questions, considering the strengths and limitations of each model and the immune cell populations of interest. © 2023 by the authors.