Detection of giardia duodenalis antigen in human fecal eluates by enzyme-linked immunosorbent assay using polyclonal antibodies
The present study developed and standardized an enzime-linked immunosorbent assay (ELISA) to detect Giardia antigen in feces using rabbit polyclonal antibodies. Giardia cysts were purified from human fecal samples by sucrose and percoll gradients. Gerbils (Meriones unguiculatus) were infected to obt...
- Autores:
-
Duque Beltrán, Sofía
Nicholls Orejuela, Rubén Santiago
Arévalo Jamaica, Adriana
Guerrero-Lozano, Rafael
Montenegro, Sonia
James, Mark A
- Tipo de recurso:
- Fecha de publicación:
- 2002
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/1661
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/1661
http://doi.org/10.1590/S0074-02762002000800018
- Palabra clave:
- Ensayo de inmunoadsorción enzimática
Formación de anticuerpos
Pruebas inmunológicas
Giardia
Feces
Antigen
- Rights
- License
- Attribution-NonCommercial 4.0 International