Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient
This study investigated the effect of hypochlorous acid (HOCl) rinses and chlorhexidine (CHX) on the bacterial viability of S. mutans, A. israelii, P. gingivalis, A. actinomycetemcomitans, E. corrodens, C. rectus, K. oxytoca, K. pneumoniae and E. cloacae. The percentage of live bacteria was tested b...
- Autores:
-
Castillo, Diana M.
Castillo, Yormaris
Jola, Johana
Calderón, Justo Leonardo
Delgadillo, Nathaly Andrea
Neuta, Yineth
Lafaurie, Gloria Ines
- Tipo de recurso:
- Fecha de publicación:
- 2015
- Institución:
- Universidad El Bosque
- Repositorio:
- Repositorio U. El Bosque
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unbosque.edu.co:20.500.12495/1579
- Acceso en línea:
- http://hdl.handle.net/20.500.12495/1579
http://dx.doi.org/10.1590/0103-6440201300388
- Palabra clave:
- Streptococcus mutans
Proteobacteria
Ácido hipocloroso
Hypochlorous acid
Chlorhexidine
Bacterial viability
- Rights
- License
- Attribution-NonCommercial 4.0 International
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oai:repositorio.unbosque.edu.co:20.500.12495/1579 |
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Repositorio U. El Bosque |
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|
dc.title.spa.fl_str_mv |
Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient |
title |
Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient |
spellingShingle |
Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient Streptococcus mutans Proteobacteria Ácido hipocloroso Hypochlorous acid Chlorhexidine Bacterial viability |
title_short |
Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient |
title_full |
Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient |
title_fullStr |
Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient |
title_full_unstemmed |
Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient |
title_sort |
Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredient |
dc.creator.fl_str_mv |
Castillo, Diana M. Castillo, Yormaris Jola, Johana Calderón, Justo Leonardo Delgadillo, Nathaly Andrea Neuta, Yineth Lafaurie, Gloria Ines |
dc.contributor.author.none.fl_str_mv |
Castillo, Diana M. Castillo, Yormaris Jola, Johana Calderón, Justo Leonardo Delgadillo, Nathaly Andrea Neuta, Yineth Lafaurie, Gloria Ines |
dc.contributor.orcid.none.fl_str_mv |
Lafaurie, Gloria Ines [0000-0003-3986-0625] Delgadillo, Nathaly Andrea [0000-0001-8842-549X] |
dc.subject.decs.spa.fl_str_mv |
Streptococcus mutans Proteobacteria Ácido hipocloroso |
topic |
Streptococcus mutans Proteobacteria Ácido hipocloroso Hypochlorous acid Chlorhexidine Bacterial viability |
dc.subject.keywords.spa.fl_str_mv |
Hypochlorous acid Chlorhexidine Bacterial viability |
description |
This study investigated the effect of hypochlorous acid (HOCl) rinses and chlorhexidine (CHX) on the bacterial viability of S. mutans, A. israelii, P. gingivalis, A. actinomycetemcomitans, E. corrodens, C. rectus, K. oxytoca, K. pneumoniae and E. cloacae. The percentage of live bacteria was tested by fluorescence method using Live/Dead kit(r) and BacLight (Molecular Probes(r)) and compared between groups by the Kruskal-Wallis and U Mann-Whitney tests with Bonferroni correction (p value<0.012). The effect of HOCl and CHX on total proteins of P. gingivalis and S. mutans was determined by SDS-PAGE. CHX showed a higher efficacy than HOCl against S. mutans, A. israelii, E. corrodens and E. cloacae (p<0.001) while HOCl was more effective than CHX against P. gingivalis, A. actinomycetemcomitans, C. rectus and K. oxytoca (p=0.001). CHX and HOCl had similar efficacy against K. pneumoniae. Proteins of P. gingivalis and S. mutans were affected similarly by HOCl and CHX. HOCl reduced the bacterial viability especially in periodontopathic bacteria, which may support its use in the control of subgingival biofilm in periodontal patients. |
publishDate |
2015 |
dc.date.issued.none.fl_str_mv |
2015 |
dc.date.accessioned.none.fl_str_mv |
2019-07-31T18:49:07Z |
dc.date.available.none.fl_str_mv |
2019-07-31T18:49:07Z |
dc.type.spa.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.local.spa.fl_str_mv |
artículo |
dc.identifier.issn.none.fl_str_mv |
1806-4760 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12495/1579 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201300388 |
dc.identifier.instname.spa.fl_str_mv |
instname:Universidad El Bosque |
dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional Universidad El Bosque |
dc.identifier.repourl.none.fl_str_mv |
repourl:https://repositorio.unbosque.edu.co |
identifier_str_mv |
1806-4760 instname:Universidad El Bosque reponame:Repositorio Institucional Universidad El Bosque repourl:https://repositorio.unbosque.edu.co |
url |
http://hdl.handle.net/20.500.12495/1579 http://dx.doi.org/10.1590/0103-6440201300388 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.spa.fl_str_mv |
Brazilian Dental Journal, 1806-4760, Vol. 26, Num. 5, 2015 |
dc.relation.uri.none.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402015000500519&lng=en&nrm=iso&tlng=en |
dc.rights.*.fl_str_mv |
Attribution-NonCommercial 4.0 International |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.local.spa.fl_str_mv |
Acceso abierto |
dc.rights.accessrights.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf369 |
dc.rights.creativecommons.none.fl_str_mv |
2015 |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ Acceso abierto http://purl.org/coar/access_right/c_abf369 2015 http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.publisher.journal.spa.fl_str_mv |
Brazilian Dental Journal |
institution |
Universidad El Bosque |
bitstream.url.fl_str_mv |
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Castillo, Diana M.Castillo, YormarisJola, JohanaCalderón, Justo LeonardoDelgadillo, Nathaly AndreaNeuta, YinethLafaurie, Gloria InesLafaurie, Gloria Ines [0000-0003-3986-0625]Delgadillo, Nathaly Andrea [0000-0001-8842-549X]2019-07-31T18:49:07Z2019-07-31T18:49:07Z20151806-4760http://hdl.handle.net/20.500.12495/1579http://dx.doi.org/10.1590/0103-6440201300388instname:Universidad El Bosquereponame:Repositorio Institucional Universidad El Bosquerepourl:https://repositorio.unbosque.edu.coapplication/pdfengFundação Odontológica de Ribeirão PretoBrazilian Dental JournalBrazilian Dental Journal, 1806-4760, Vol. 26, Num. 5, 2015http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402015000500519&lng=en&nrm=iso&tlng=enAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/Acceso abiertohttp://purl.org/coar/access_right/c_abf3692015http://purl.org/coar/access_right/c_abf2Viability and effects on bacterial proteins by oral rinses with hypochlorous acid as active ingredientarticleartículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Streptococcus mutansProteobacteriaÁcido hipoclorosoHypochlorous acidChlorhexidineBacterial viabilityThis study investigated the effect of hypochlorous acid (HOCl) rinses and chlorhexidine (CHX) on the bacterial viability of S. mutans, A. israelii, P. gingivalis, A. actinomycetemcomitans, E. corrodens, C. rectus, K. oxytoca, K. pneumoniae and E. cloacae. The percentage of live bacteria was tested by fluorescence method using Live/Dead kit(r) and BacLight (Molecular Probes(r)) and compared between groups by the Kruskal-Wallis and U Mann-Whitney tests with Bonferroni correction (p value<0.012). The effect of HOCl and CHX on total proteins of P. gingivalis and S. mutans was determined by SDS-PAGE. CHX showed a higher efficacy than HOCl against S. mutans, A. israelii, E. corrodens and E. cloacae (p<0.001) while HOCl was more effective than CHX against P. gingivalis, A. actinomycetemcomitans, C. rectus and K. oxytoca (p=0.001). CHX and HOCl had similar efficacy against K. pneumoniae. Proteins of P. gingivalis and S. mutans were affected similarly by HOCl and CHX. HOCl reduced the bacterial viability especially in periodontopathic bacteria, which may support its use in the control of subgingival biofilm in periodontal patients.ORIGINALCastillo D.M., Castillo Y., delgadillo N.A., Neuta Y., Jola J., Calderón J.L., Lafaurie G.I..pdfCastillo D.M., Castillo Y., delgadillo N.A., Neuta Y., Jola J., Calderón J.L., Lafaurie G.I..pdfapplication/pdf752467https://repositorio.unbosque.edu.co/bitstreams/b06e1189-666f-49b7-9633-3cab157f0d70/downloadc6353371c3c041f2796eedd5c3cf3587MD54CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908https://repositorio.unbosque.edu.co/bitstreams/14ba650a-d426-4d7f-ac0c-0ea55cd417f6/download0175ea4a2d4caec4bbcc37e300941108MD55LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.unbosque.edu.co/bitstreams/0a5e11fa-ad95-4002-a7ac-756cd010ec5b/download8a4605be74aa9ea9d79846c1fba20a33MD56THUMBNAILCastillo D.M., Castillo Y., delgadillo N.A., Neuta Y., Jola J., Calderón J.L., Lafaurie G.I..pdf.jpgCastillo D.M., Castillo Y., delgadillo N.A., Neuta Y., Jola J., Calderón J.L., Lafaurie G.I..pdf.jpgIM Thumbnailimage/jpeg8643https://repositorio.unbosque.edu.co/bitstreams/0adcd780-5e7b-4ae4-946f-1a246e8d7c12/downloadcf043e0956066f414da744ce509b90e6MD57TEXTCastillo D.M., Castillo Y., delgadillo N.A., Neuta Y., Jola J., Calderón J.L., Lafaurie G.I..pdf.txtCastillo D.M., Castillo Y., delgadillo N.A., Neuta Y., Jola J., Calderón J.L., Lafaurie G.I..pdf.txtExtracted texttext/plain27054https://repositorio.unbosque.edu.co/bitstreams/dc3b0139-f25f-4af4-a893-349152cd8256/downloadb8f80369326f1af7c1e39ea9ba0363a0MD5820.500.12495/1579oai:repositorio.unbosque.edu.co:20.500.12495/15792024-02-06 22:35:55.899http://creativecommons.org/licenses/by-nc/4.0/Attribution-NonCommercial 4.0 Internationalopen.accesshttps://repositorio.unbosque.edu.coRepositorio Institucional Universidad El Bosquebibliotecas@biteca.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 |