Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)

ilustraciones, diagramas

Autores:: España Amórtegui, Julio César

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2024

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)
dc.title.translated.eng.fl_str_mv	Evaluation of pesticide residues, mycotoxins and processing contaminants as key descriptors of chemical safety in the post-harvest of the coffee production chain in the Tolima region using analytical strategies based on Stable Isotope Labeling (SIL).
title	Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)
spellingShingle	Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL) 540 - Química y ciencias afines::543 - Química analítica Desechos agrícolas Inocuidad alimentaria Limites máximos de residuos agricultural wastes food safety maximum residue limits Ochratoxin A Acrylamide Pesticides Polycyclic aromatic hydrocarbons Stable isotope labeling Validation Eco-friendly Ocratoxin A Plaguicidas Hidrocarburos aromáticos policíclicos Marcado con isótopos estables Validación Ambientalmente amigable Acrilamida
title_short	Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)
title_full	Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)
title_fullStr	Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)
title_full_unstemmed	Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)
title_sort	Evaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)
dc.creator.fl_str_mv	España Amórtegui, Julio César
dc.contributor.advisor.spa.fl_str_mv	Guerrero Dallos, Jairo Arturo
dc.contributor.author.spa.fl_str_mv	España Amórtegui, Julio César
dc.contributor.researchgroup.spa.fl_str_mv	Residualidad y destino ambiental de plaguicidas en sistemas agrícolas
dc.contributor.orcid.spa.fl_str_mv	0000-0003-3566-5689
dc.contributor.cvlac.spa.fl_str_mv	0001104837
dc.contributor.scopus.spa.fl_str_mv	56089546400
dc.contributor.googlescholar.spa.fl_str_mv	nOAM6KsAAAAJ&hl=es&oi=ao
dc.subject.ddc.spa.fl_str_mv	540 - Química y ciencias afines::543 - Química analítica
topic	540 - Química y ciencias afines::543 - Química analítica Desechos agrícolas Inocuidad alimentaria Limites máximos de residuos agricultural wastes food safety maximum residue limits Ochratoxin A Acrylamide Pesticides Polycyclic aromatic hydrocarbons Stable isotope labeling Validation Eco-friendly Ocratoxin A Plaguicidas Hidrocarburos aromáticos policíclicos Marcado con isótopos estables Validación Ambientalmente amigable Acrilamida
dc.subject.agrovoc.spa.fl_str_mv	Desechos agrícolas Inocuidad alimentaria Limites máximos de residuos
dc.subject.agrovoc.eng.fl_str_mv	agricultural wastes food safety maximum residue limits
dc.subject.proposal.eng.fl_str_mv	Ochratoxin A Acrylamide Pesticides Polycyclic aromatic hydrocarbons Stable isotope labeling Validation Eco-friendly
dc.subject.proposal.spa.fl_str_mv	Ocratoxin A Plaguicidas Hidrocarburos aromáticos policíclicos Marcado con isótopos estables Validación Ambientalmente amigable Acrilamida
description	ilustraciones, diagramas
publishDate	2024
dc.date.accessioned.none.fl_str_mv	2024-05-23T21:13:44Z
dc.date.available.none.fl_str_mv	2024-05-23T21:13:44Z
dc.date.issued.none.fl_str_mv	2024-05-23
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/86151
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/86151 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.coverage.country.spa.fl_str_mv	Colombia
dc.coverage.region.spa.fl_str_mv	Tolima
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Doctorado en Ciencias - Química
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Guerrero Dallos, Jairo Arturoa946cb1c8ed0e3f18e4295de585db564600España Amórtegui, Julio Césarb7ffa10459f0992bb7f28fbc2e5a2939Residualidad y destino ambiental de plaguicidas en sistemas agrícolas0000-0003-3566-5689000110483756089546400nOAM6KsAAAAJ&hl=es&oi=ao2024-05-23T21:13:44Z2024-05-23T21:13:44Z2024-05-23https://repositorio.unal.edu.co/handle/unal/86151Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasSe presenta el desarrollo y validación de un procedimiento simple, integral y amigable con el medio ambiente para determinar residuos de plaguicidas, contaminantes naturales y de procesamiento en el café tostado por LC-HRMS, e hidrocarburos aromáticos policíclicos (HAPs) vía GC-MS/MS. Para la primera técnica se extraen plaguicidas y micotoxinas con acetato de etilo, con una partición simultánea de acrilamida (AA) en fase acuosa en la misma porción analítica. Una microextracción en fase sólida dispersiva 'en tubo' (dSPME) retiene la AA para inyectarla de forma independiente de los compuestos de la fase orgánica. A través de novedoso método de cromatografía líquida de espectrometría de masas de alta resolución (LC-HRMS) se cuantificaron 186 compuestos a 10 µg/kg, 226 a 5 µg/kg y la AA a 200 µg/kg, para un total de 414 moléculas con recuperación (70%-120%) y precisiones aceptables (RSD<20%). Se confirmó la presencia de clorpirifos, AA y ocratoxina A (OTA) en muestras de diferente origen por debajo del límite de cuantificación. No hubo evidencia de enmascaramiento de OTA durante el almacenamiento del café; sin embargo, se evidenció condensación con glucosa durante experimentos de procesamiento térmico con sacarosa mediante el uso de marcado con isótopos estables (SIL). No se encontraron conjugados en muestras de café tostado ni torrefacto. Entre las alternativas revisadas para determinar los HAPs, una transesterificación precedió la cuantificación que fue validada para un grupo de HAPs que incluyó los 4 indicadores en la regulación vigente. Algunos hallazgos en muestras comerciales fueron cuantificados por GC-MS/MS y confirmados por LC-HRMS. (Texto tomado de la fuente).The development and validation of a simple, comprehensive, and environmentally friendly procedure is presented to determine pesticide residues, natural and processing contaminants in roasted coffee by LC-HRMS, and polycyclic aromatic hydrocarbons (PAHs) via GC-MS/ MS. For the first technique, pesticides and mycotoxins are extracted with ethyl acetate, along a simultaneous partition of acrylamide (AA) in the aqueous phase in the same analytical portion. An 'in-tube' dispersive solid phase microextraction (dSPME) retains the AA for injection independently of the organic phase compounds. A novel liquid chromatography high resolution mass spectrometry (LC-HRMS) method allows the quantification of 186 compounds at 10 µg/kg, 226 at 5 µg/kg and AA at 200 µg/kg for a total of 414 molecules with recovery (70%-120%) and acceptable precisions (RSD<20%). The presence of chlorpyrifos, AA and OTA was confirmed in samples of different origins below the limit of quantification. There was no evidence of OTA masking during coffee storage; however, condensation with glucose molecules was evident during thermal processing experiments with sucrose using stable isotope labeling (SIL). No conjugates were found in roasted or torrefacto coffee samples. Among the tested alternatives to determine PAHs, a transesterification process allowed a determination validated for a group that included selected indicative 4 PAHs in the current regulation. Some findings in commercial samples were quantified by GC-MS/MS and confirmed by LC-HRMS.DoctoradoDoctor en Ciencias - QuímicaQuímica agroalimentaria y ambientalxvi, 173 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Doctorado en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines::543 - Química analíticaDesechos agrícolasInocuidad alimentariaLimites máximos de residuosagricultural wastesfood safetymaximum residue limitsOchratoxin AAcrylamidePesticidesPolycyclic aromatic hydrocarbonsStable isotope labelingValidationEco-friendlyOcratoxin APlaguicidasHidrocarburos aromáticos policíclicosMarcado con isótopos establesValidaciónAmbientalmente amigableAcrilamidaEvaluación de residuos de plaguicidas, micotoxinas y contaminantes de procesamiento como descriptores claves de inocuidad química en la poscosecha de la cadena de producción cafetera de la región del Tolima empleando estrategias analíticas basadas en marcado de isótopos estables (SIL)Evaluation of pesticide residues, mycotoxins and processing contaminants as key descriptors of chemical safety in the post-harvest of the coffee production chain in the Tolima region using analytical strategies based on Stable Isotope Labeling (SIL).Trabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDColombiaTolimahttp://vocab.getty.edu/page/tgn/1000784Daviron, B., & Ponte, S. 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Anal Chem 92:10076–10082. https://doi.org/10.1021/acs.analchem.0c01887Becas de Excelencia Doctoral del BicentenarioCOL 5025 IAEA project Coordinated Research Project D52039/Research contract 22177Ministerio de ciencia, innovación y tecnología (MinCiencias)International Atomic Energy Agency (IAEA)EstudiantesGrupos comunitariosInvestigadoresPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86151/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1019016538.2024.pdf1019016538.2024.pdfTesis de Doctorado en Ciencias - Químicaapplication/pdf11969859https://repositorio.unal.edu.co/bitstream/unal/86151/2/1019016538.2024.pdf8a73bc191c9c52de16a384b8c016db5cMD52unal/86151oai:repositorio.unal.edu.co:unal/861512024-05-23 16:15:19.249Repositorio Institucional Universidad Nacional de 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