Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method
This study was developed with the aim of evaluating the pH, enzymatic complex load and temperature effects on the saccharification of pretreated cassava stalks (CS) using the response surface methodology (RSM). The factor levels evaluated were temperature 35 - 40°C, pH 4.0 - 5.0 and dose of enzymati...
- Autores:
-
Castaño P., Hader
Reales A., Juan G.
Zapata M., José
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/58520
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/58520
http://bdigital.unal.edu.co/55303/
- Palabra clave:
- 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
starch digestion
Manihot esculenta Crantz
enzymolysis
lignocellulose
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Castaño P., Haderce44f804-2d7c-4bc2-94d9-f1f851cea94f300Reales A., Juan G.e7880c2d-9cf3-4003-8046-c037ac8086eb300Zapata M., José2b675077-6657-44d3-a69c-c496d8899bef3002019-07-02T14:18:55Z2019-07-02T14:18:55Z2015-05-01ISSN: 2357-3732https://repositorio.unal.edu.co/handle/unal/58520http://bdigital.unal.edu.co/55303/This study was developed with the aim of evaluating the pH, enzymatic complex load and temperature effects on the saccharification of pretreated cassava stalks (CS) using the response surface methodology (RSM). The factor levels evaluated were temperature 35 - 40°C, pH 4.0 - 5.0 and dose of enzymatic complex Accellerase 1500™ 2.9 - 14.5 FPU/g of substrate. The reducing sugar (RS) response was used. The pH was controlled through the use of hydrochloric acid and sodium hydroxide solutions and the system was shaken orbitally at 120 rpm with a solids loading of 10% w/v. The fitted model showed that the optimal operating conditions were: pH 4.0, 38°C and enzyme dose of 14.5 FPU/g substrate, reaching a sugar concentration of 18.4 g L-1.application/pdfspaUniversidad Nacional de Colombiahttp://www.revistas.unal.edu.co/index.php/agrocol/article/view/50115Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaCastaño P., Hader and Reales A., Juan G. and Zapata M., José (2015) Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method. Agronomía Colombiana, 33 (2). pp. 238-243. ISSN 2357-373257 Ciencias de la vida; Biología / Life sciences; biology58 Plantas / Plantsstarch digestionManihot esculenta CrantzenzymolysislignocelluloseEnzymatic hydrolysis of cassava stalk pretreated with the alkaline methodArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINALagron.colomb.v33n2.50115.pdfapplication/pdf190964https://repositorio.unal.edu.co/bitstream/unal/58520/1/agron.colomb.v33n2.50115.pdf440e30369dc90dd389829fa04c5cccdaMD51THUMBNAILagron.colomb.v33n2.50115.pdf.jpgagron.colomb.v33n2.50115.pdf.jpgGenerated Thumbnailimage/jpeg9126https://repositorio.unal.edu.co/bitstream/unal/58520/2/agron.colomb.v33n2.50115.pdf.jpg4153c8bbc4816371a7e1b3bc30729503MD52unal/58520oai:repositorio.unal.edu.co:unal/585202023-03-28 23:07:36.538Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
| dc.title.spa.fl_str_mv |
Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method |
| title |
Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method |
| spellingShingle |
Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method 57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants starch digestion Manihot esculenta Crantz enzymolysis lignocellulose |
| title_short |
Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method |
| title_full |
Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method |
| title_fullStr |
Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method |
| title_full_unstemmed |
Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method |
| title_sort |
Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method |
| dc.creator.fl_str_mv |
Castaño P., Hader Reales A., Juan G. Zapata M., José |
| dc.contributor.author.spa.fl_str_mv |
Castaño P., Hader Reales A., Juan G. Zapata M., José |
| dc.subject.ddc.spa.fl_str_mv |
57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants |
| topic |
57 Ciencias de la vida; Biología / Life sciences; biology 58 Plantas / Plants starch digestion Manihot esculenta Crantz enzymolysis lignocellulose |
| dc.subject.proposal.spa.fl_str_mv |
starch digestion Manihot esculenta Crantz enzymolysis lignocellulose |
| description |
This study was developed with the aim of evaluating the pH, enzymatic complex load and temperature effects on the saccharification of pretreated cassava stalks (CS) using the response surface methodology (RSM). The factor levels evaluated were temperature 35 - 40°C, pH 4.0 - 5.0 and dose of enzymatic complex Accellerase 1500™ 2.9 - 14.5 FPU/g of substrate. The reducing sugar (RS) response was used. The pH was controlled through the use of hydrochloric acid and sodium hydroxide solutions and the system was shaken orbitally at 120 rpm with a solids loading of 10% w/v. The fitted model showed that the optimal operating conditions were: pH 4.0, 38°C and enzyme dose of 14.5 FPU/g substrate, reaching a sugar concentration of 18.4 g L-1. |
| publishDate |
2015 |
| dc.date.issued.spa.fl_str_mv |
2015-05-01 |
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2019-07-02T14:18:55Z |
| dc.date.available.spa.fl_str_mv |
2019-07-02T14:18:55Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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Text |
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http://purl.org/redcol/resource_type/ART |
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http://purl.org/coar/resource_type/c_6501 |
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publishedVersion |
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ISSN: 2357-3732 |
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https://repositorio.unal.edu.co/handle/unal/58520 |
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http://bdigital.unal.edu.co/55303/ |
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ISSN: 2357-3732 |
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https://repositorio.unal.edu.co/handle/unal/58520 http://bdigital.unal.edu.co/55303/ |
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spa |
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spa |
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http://www.revistas.unal.edu.co/index.php/agrocol/article/view/50115 |
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Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana Agronomía Colombiana |
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Castaño P., Hader and Reales A., Juan G. and Zapata M., José (2015) Enzymatic hydrolysis of cassava stalk pretreated with the alkaline method. Agronomía Colombiana, 33 (2). pp. 238-243. ISSN 2357-3732 |
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Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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