Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja
Ilustraciones, tablas
- Autores:
-
Tigreros, Jaime Andres
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/85235
- Palabra clave:
- 664 - Tecnología de alimentos
Radiación electromagnética
Electromagnetic radiation
Microondas
Microwave radiation
Compuestos bioactivos
Bioactive compounds
Solanum betaceum
Color de las frutas
Fruit colour
Peroxidasa
Fitoquímicos
Tamarillo
DPPH
Vitamina C
Peroxidase
Phytochemicals
Tamarillo
DPPH
Vitamin C
Vitamins B
Vitaminas B
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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dc.title.spa.fl_str_mv |
Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja |
dc.title.translated.eng.fl_str_mv |
Effect of microwave treatment on peroxidase enzyme, bioactive compounds and color in tamarillo (Solanum betaceum Cav.) red variety |
title |
Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja |
spellingShingle |
Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja 664 - Tecnología de alimentos Radiación electromagnética Electromagnetic radiation Microondas Microwave radiation Compuestos bioactivos Bioactive compounds Solanum betaceum Color de las frutas Fruit colour Peroxidasa Fitoquímicos Tamarillo DPPH Vitamina C Peroxidase Phytochemicals Tamarillo DPPH Vitamin C Vitamins B Vitaminas B |
title_short |
Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja |
title_full |
Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja |
title_fullStr |
Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja |
title_full_unstemmed |
Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja |
title_sort |
Efecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad roja |
dc.creator.fl_str_mv |
Tigreros, Jaime Andres |
dc.contributor.advisor.none.fl_str_mv |
Ordoñez Santos, Luis Eduardo Martínez Girón, Jader |
dc.contributor.author.none.fl_str_mv |
Tigreros, Jaime Andres |
dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Procesos Agroindustriales (Gipa) |
dc.contributor.orcid.spa.fl_str_mv |
http://orcid.org/0000-0002-6883-7064 |
dc.subject.ddc.spa.fl_str_mv |
664 - Tecnología de alimentos |
topic |
664 - Tecnología de alimentos Radiación electromagnética Electromagnetic radiation Microondas Microwave radiation Compuestos bioactivos Bioactive compounds Solanum betaceum Color de las frutas Fruit colour Peroxidasa Fitoquímicos Tamarillo DPPH Vitamina C Peroxidase Phytochemicals Tamarillo DPPH Vitamin C Vitamins B Vitaminas B |
dc.subject.agrovoc.none.fl_str_mv |
Radiación electromagnética Electromagnetic radiation Microondas Microwave radiation Compuestos bioactivos Bioactive compounds Solanum betaceum Color de las frutas Fruit colour |
dc.subject.proposal.spa.fl_str_mv |
Peroxidasa Fitoquímicos Tamarillo DPPH Vitamina C Peroxidase Phytochemicals Tamarillo DPPH Vitamin C Vitamins B |
dc.subject.proposal.none.fl_str_mv |
Vitaminas B |
description |
Ilustraciones, tablas |
publishDate |
2023 |
dc.date.issued.none.fl_str_mv |
2023 |
dc.date.accessioned.none.fl_str_mv |
2024-01-11T20:50:12Z |
dc.date.available.none.fl_str_mv |
2024-01-11T20:50:12Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
status_str |
acceptedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/85235 |
dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
url |
https://repositorio.unal.edu.co/handle/unal/85235 https://repositorio.unal.edu.co/ |
identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.references.spa.fl_str_mv |
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The effects of microwave blanching conditions on carrot slices: optimization and comparison. Journal of Food Processing and Preservation, 39(6), 2188–2196. https://doi.org/10.1111/jfpp.12463 Behera, G., Rayaguru, K., & Nayak, P. K. (2017). Effect of Microwave Blanching on Slice Thickness and Quality Analysis of Star Fruit. Current Research in Nutrition and Food Science, 5(3). Bloom, D. ., Cafiero, E. ., Jané-Llopis, E., Abrahams-Gessel, S., Bloom, L. ., Fathima, S., … Weinstein, C. (2011). The Global Economic Burden of Non-communicable Diseases. Geneva: World Economic Forum. Bohs, L. (1995). Transfer of Cyphomandra (Solanaceae) and Its Species to Solanum. Source: Taxon (Vol. 44). https://doi.org/http://bohs.biology.utah.edu/PDFs/Lynn/Bohs-1995.pdf Bonnechère, A., Hanot, V., Jolie, R., Hendrickx, M., Bragard, C., Bedoret, T., & Loco, J. Van. (2012). Effect of household and industrial processing on levels of five pesticide residues and two degradation products in spinach. 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Food Science and Technology, 40(June), 273–279. https://doi.org/10.1590/fst.09419 Dorantes-Alvarez, L., Jaramillo-Flores, E., González, K., Martinez, R., & Parada, L. (2011). Blanching peppers using microwaves. Procedia Food Science, 1, 178–183. https://doi.org/10.1016/j.profoo.2011.09.028 Espin, S., Gonzalez-Manzano, S., Taco, V., Poveda, C., Ayuda-Durán, B., Gonzalez-Paramas, A. M., & Santos-Buelga, C. (2016). Phenolic composition and antioxidant capacity of yellow and purple-red Ecuadorian cultivars of tree tomato (Solanum betaceum Cav.). Food Chemistry, 194, 1073–1080. https://doi.org/10.1016/j.foodchem.2015.07.131 Francis, F. J. (1995). Quality as influenced by color. Food Quality and Preference, 6(3), 149–155. https://doi.org/10.1016/0950-3293(94)00026-R Frazier, W., & Westhoff, D. (1993). Microbiología de los alimentos (4th ed.). Zaragoza: Acribia, S.A. https://doi.org/http://148.206.53.84/tesiuami/Libros/L33.pdf García. (2008). Manual de manejo cosecha y poscosecha del tomate de árbol. Corpoica. Gliszczyńska-Świgło, A., Ciska, E., Pawlak-Lemańska, K., Chmielewski, J., Borkowski, T., & Tyrakowska, B. (2006). Changes in the content of health-promoting compounds and antioxidant activity of broccoli after domestic processing. Food Additives and Contaminants, 23(11), 1088–1098. https://doi.org/10.1080/02652030600887594 Gonçalves, E. M., Pinheiro, J., Abreu, M., Brandão, T. R. S., & Silva, C. L. M. (2010). Carrot (Daucus carota L.) peroxidase inactivation, phenolic content and physical changes kinetics due to blanching. Journal of Food Engineering, 97(4), 574–581. https://doi.org/10.1016/j.jfoodeng.2009.12.005 Hadidi, M., Ibarz, A., Conde, J., & Pagan, J. (2019). Optimisation of steam blanching on enzymatic activity, color and protein degradation of alfalfa (Medicago sativa) to improve some quality characteristics of its edible protein. Food Chemistry, 276(October 2018), 591–598. https://doi.org/10.1016/j.foodchem.2018.10.049 Huang, Y., Sheng, J., Yang, F., & Hu, Q. (2007). Effect of enzyme inactivation by microwave and oven heating on preservation quality of green tea. Journal of Food Engineering, 78(2), 687–692. https://doi.org/10.1016/j.jfoodeng.2005.11.007 Huong, N. T. T., Thinh, P., Long, D. V., Long, H. B., Dat, U. T., Phat, D. T., Nhut, P. T., Phuc, T. B., & Nhi, T. T. Y. (2022). Effects of microwave and ultrasound treatment on vitamin C, polyphenols and antioxidant activity of mango (Mangifera Indica) during low temperature drying. Materials Today: Proceedings, 59, 781–786. https://doi.org/10.1016/j.matpr.2021.12.581 Hurtado, N. H., Morales, A. L., González-Miret, M. L., Escudero-Gilete, M. L., & Heredia, F. J. (2009). Colour, pH stability and antioxidant activity of anthocyanin rutinosides isolated from tamarillo fruit (Solanum betaceum Cav.). Food Chemistry, 117(1), 88–93. https://doi.org/10.1016/j.foodchem.2009.03.081 Instituto Colombiano Normas Técnicas (ICONTEC): NTC 4105: Frutas frescas. Tomate de árbol, Especificaciones. Bogotá (Colombia): 1997,15 p. Ismail, A., Marjan, Z. M., & Foong, C. W. (2004). Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry, 87(4), 581–586. https://doi.org/10.1016/j.foodchem.2004.01.010 Jabbar, S., Abid, M., Hu, B., Wu, T., Muhammad, M., Lei, S., … Zeng, X. (2014). Quality of carrot juice as in fl uenced by blanching and sonication treatments. LWT - Food Science and Technology, 55(1), 16–21. https://doi.org/10.1016/j.lwt.2013.09.007 Kutlu, N., Pandiselvam, R., Saka, I., Kamiloglu, A., Sahni, P., & Kothakota, A. (2022). Impact of different microwave treatments on food texture. Journal of Texture Studies, 53(6), 709-736. https://doi.org/10.1111/jtxs.12635. Lee, F. (1958). The Blanching Process. Advances in Food Research, 8(C), 63–109. https://doi.org/10.1016/S0065-2628(08)60018-X Lisiewska, Jacek, S., Skoczen-Stupska, & Kmiecik, W. (2009). Content of amino acids and the quality of protein in Brussels sprouts , both raw and prepared for consumption. Interna t Ional Jo Urna l o f Refrigeration, 32, 272–278. https://doi.org/10.1016/j.ijrefrig.2008.05.011 Lisiewska, & Kmiecik. (1996). Effects of level of nitrogen fertilizer, processing conditions and period of storage of frozen broccoli and cauliflower on vitamin C retention. Food Chemistry, 57(2), 267–270. https://doi.org/10.1016/0308-8146(95)00218-9 MADR. (2018). Estadísticas del cultivo de tomate de árbol. Retrieved October 26, 2018, from http://www.agronet.gov.co/Documents/TOMATE DE ARBOL2016.pdf MADR, DNP, DANE, & ASOHOFRUCOL. (2004). I Censo nacional de 10 frutas agroindustriales y promisorias: resultados 2004. Retrieved October 25, 2018, from http://bibliotecadigital.agronet.gov.co/handle/11348/4459 Marx, M., Stuparic, M., Schieber, A., & Carle, R. (2003). Effects of thermal processing on trans–cis-isomerization of β-carotene in carrot juices and carotene-containing preparations. Food Chemistry, 83, 609–617. https://doi.org/doi:10.1016/S0308-8146(03)00255-3 Mukherjee, S., & Chattopadhyay, P. K. (2007). Whirling bed blanching of potato cubes and its effects on product quality. Journal of Food Engineering, 78(1), 52–60. https://doi.org/10.1016/j.jfoodeng.2005.09.001 Nakilcioglu-Taş, E., & Otleş, S. (2018). Degradation kinetics of bioactive compounds and antioxidant capacity of Brussels sprouts during microwave processing. International Journal of Food Properties, 20(3), S2798–S2809. https://doi.org/10.1080/10942912.2017.1375944 Ordóñez, R. M., Vattuone, M. A., & Isla, M. I. (2005). Changes in carbohydrate content and related enzyme activity during Cyphomandra betacea (Cav.) Sendtn. fruit maturation. Postharvest Biology and Technology, 35(3), 293-301. https://doi.org/10.1016/j.postharvbio.2004.09.006 OMS. (2016). Enfermedades No Transmisibles. Bogotá, Colombia. https://doi.org/http://www.who.int/nmh/countries/col_en.pdf?ua=1 Oszmiański, J., Wolniak, M., Wojdyło, A., & Wawer, I. (2008). Influence of apple purée preparation and storage on polyphenol contents and antioxidant activity. Food Chemistry. https://doi.org/10.1016/j.foodchem.2007.10.003 Palma-Orozco, G., Sampedro, J. G., Ortiz-Moreno, A., & Nájera, H. (2012). In situ Inactivation of Polyphenol Oxidase in Mamey Fruit (Pouteria sapota) by Microwave Treatment. Journal of Food Science, 77(4), 359–365. https://doi.org/10.1111/j.1750-3841.2012.02632.x Phungamngoen, C., Chiewchan, N., & Devahastin, S. (2013). Effects of various pretreatments and drying methods on Salmonella resistance and physical properties of cabbage. 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Tang (Ed.), The microwave processing of foods (primera ed, pp. 22–38). Washington: Woodhead Publishing Limited. Severini, C., Baiano, A., De Pilli, T., Carbone, B., & Derossi, A. (2005). Combined treatments of blanching and dehydration: study on potato cubes. Journal of Food Engineering, 68, 289–296. https://doi.org/10.1016/j.jfoodeng.2004.05.045 Stamatopoulos, K., Katsoyannos, E., Chatzilazarou, A., & Konteles, S. J. (2012). Improvement of oleuropein extractability by optimising steam blanching process as pre-treatment of olive leaf extraction via response surface methodology. Food Chemistry, 133(2), 344–351. https://doi.org/10.1016/j.foodchem.2012.01.038 Tao, Y. M., Wang, S., Luo, H. L., & Yan, W. W. (2018). Peroxidase from jackfruit: Purification, characterization and thermal inactivation. International Journal of Biological Macromolecules, 114, 898–905. https://doi.org/10.1016/j.ijbiomac.2018.04.007 Tao, Y. M., Yao, L. Y., Qin, Q. Y., & Shen, W. (2013). Purification and characterization of polyphenol oxidase from jackfruit (Artocarpus heterophyllus) bulbs. Journal of Agricultural and Food Chemistry, 61(51), 12662–12669. https://doi.org/10.1021/jf403828e The High Level Panel of Experts on Food Security and Nutrition. (2017). Nutrition and food systems. Roma. Tomadoni, B., Cassani, L., Viacava, G., Del, M., Moreira, R., & Ponce, A. (2017). Effect of ultrasound and storage time on quality attributes of strawberry juice. Journal of Food Process Engineering, (November 2016), 1–8. https://doi.org/10.1111/jfpe.12533 Uddin, M. S., Hawlader, M. N. A., & Zhou, L. (2001). Drying Technology : An kinetics of ascorbic acid degradation in dried kiwifruits during storage. Drying Technology, 19(2)(2001), 437–446. https://doi.org/10.1081/DRT-100102916 Valdez, J. (2018). Rangos de conductividad eléctrica en semilla de tomate de árbol (Solanum betaceum Cav.) utilizando el equipo SAD 9000-S. Universidad Central del Ecuador. Verbeyst, L., Bogaerts, R., Van der Plancken, I., Hendrickx, M., & Van Loey, A. (2013). Modelling of Vitamin C Degradation during Thermal and High-Pressure Treatments of Red Fruit. Food and Bioprocess Technology, 6(4), 1015–1023. https://doi.org/10.1007/s11947-012-0784-y Yadav, N., Saini, P., Kaur, D., Gupta, V. K., Kaundal, B., Kumar, R., & Mishra, P. (2023). Blanching Effect on Nutritionally Important Starch Fractions of Selected Processing Potato cultivars. Food Chemistry Advances, 100404. https://doi.org/10.1016/j.focha.2023.100404. |
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Atribución-NoComercial-SinDerivadas 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Atribución-NoComercial-SinDerivadas 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2 |
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xiv, 80 páginas |
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Universidad Nacional de Colombia |
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Palmira - Ingeniería y Administración - Maestría en Ingeniería Agroindustrial |
dc.publisher.faculty.spa.fl_str_mv |
Facultad de Ingeniería y Administración |
dc.publisher.place.spa.fl_str_mv |
Palmira, Valle del Cauca, Colombia |
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Universidad Nacional de Colombia - Sede Palmira |
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Universidad Nacional de Colombia |
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spelling |
Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ordoñez Santos, Luis Eduardo5ded52dee702906861af13aac9f9fe3bMartínez Girón, Jader6e737b8a1c7b617fdaf4a5173dc1ae79Tigreros, Jaime Andres68d1a195b1a805d60ea2dd04d00e95fbGrupo de Investigación en Procesos Agroindustriales (Gipa)http://orcid.org/0000-0002-6883-70642024-01-11T20:50:12Z2024-01-11T20:50:12Z2023https://repositorio.unal.edu.co/handle/unal/85235Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones, tablasEstablecer una relación apropiada entre la inactivación enzimática alcanzada mediante el proceso de escaldado y los impactos en nutrientes y las alteraciones en el color de los alimentos, resulta fundamental para preservar la calidad de los productos transformados. En el primer capítulo, el objetivo fue estudiar los efectos del escaldado a diferentes niveles de potencia de microondas (300, 600, 900 y 1200 W) por tiempos (15, 30, 45 y 60 segundos) sobre la inactivación de peroxidasa presente en este fruto. En comparación con el tratamiento control, el escaldado con microondas logró disminuir la actividad residual de peroxidasa por debajo del 10% en un tiempo de 45 segundos a una potencia de 900 W. Con esta información, en el segundo capítulo se evaluó el efecto de las condiciones de escaldado con microondas sobre los compuestos bioactivos, la actividad antioxidante y el color encontrando que el contenido de vitamina C, fenoles totales, carotenoides, actividad antioxidante, luminosidad, croma e índice de amarillamiento no mostraron diferencia significativa (p> 0,05) en comparación con el método convencional y el fresco. Por otro lado, vitaminas B1, B3, B5, B6, tonalidad y cambio de color, si presentaron diferencias significativas (p< 0,05). Basándose en estos hallazgos, es posible sugerir la utilización de energía de microondas como una alternativa al escaldado convencional, con el fin de inactivar la peroxidasa en un tiempo corto, conservar características nutricionales y de color en el tomate de árbol (Solanum betaceum Cav.) variedad roja. (Texto tomado de la fuente)Establishing an appropriate relationship between the enzymatic inactivation achieved through the blanching process and the impacts on nutrients and the alterations in the color of the food is essential to preserve the quality of the processed products. In the first chapter, the objective was to study the effects of blanching at different microwave power levels (300, 600, 900 and 1200 W) for times (15, 30, 45 and 60 seconds) on the inactivation of peroxidase present in this fruit. Compared with the control treatment, blanching with microwaves manages to reduce the residual activity of peroxidase below 10% in a time of 45 seconds at a power of 900 W. With this information, in the second chapter the effect of the microwave blanching conditions on bioactive compounds, antioxidant activity, and color, finding that the content of vitamin C, total phenols, carotenoids, antioxidant activity, lightness, chroma, and yellowing index did not show a significant difference (p> 0.05). compared to the conventional method and the fresh method. On the other hand, vitamins B1, B3, B5, B6, hue and color change did present significant differences (p< 0.05). Based on these findings, it is possible to suggest the use of microwave energy as an alternative option to conventional blanching, in order to inactivate peroxidase in a short time, preserving nutritional and color characteristics in this fruit.MaestríaMagíster en Ingeniería AgroindustrialLos frutos de tomate de árbol (Solanum betaceum Cav.) variedad roja, se obtuvieron de un mercado local de la ciudad de Palmira, Valle del Cauca, Colombia y fueron seleccionados teniendo en cuenta que estuvieran libres de daños mecánicos y microbiológicos y en estado de madurez de consumo, escogiendo solo los frutos en escala 5 y 6 acorde con la norma NTC 4105 (ICONTEC, 1997). El material colectado se llevó inmediatamente al laboratorio de Tecnología de frutas y hortalizas de la Universidad Nacional sede Palmira para su posterior análisis. Se realizó la metodología reportada por Ordoñez-Santos & Martínez-Girón (2019). Se lavaron los frutos con agua corriente, después se desinfectaron con hipoclorito de sodio a 100 ppm durante 10 min, se retiró el pedúnculo y el pericarpio, posteriormente se obtuvieron rodajas de 0.70 cm de espesor (altura) y 4.20 cm de diámetro, las cuales se sometieron a escaldado.Agroindustria de productos alimentariosIngeniería.Sede Palmiraxiv, 80 páginasapplication/pdfspaUniversidad Nacional de ColombiaPalmira - Ingeniería y Administración - Maestría en Ingeniería AgroindustrialFacultad de Ingeniería y AdministraciónPalmira, Valle del Cauca, ColombiaUniversidad Nacional de Colombia - Sede Palmira664 - Tecnología de alimentosRadiación electromagnéticaElectromagnetic radiationMicroondasMicrowave radiationCompuestos bioactivosBioactive compoundsSolanum betaceumColor de las frutasFruit colourPeroxidasaFitoquímicosTamarilloDPPHVitamina CPeroxidasePhytochemicalsTamarilloDPPHVitamin CVitamins BVitaminas BEfecto del tratamiento con microondas sobre la enzima peroxidasa, los compuestos bioactivos y el color en tomate de árbol (Solanum betaceum Cav.) variedad rojaEffect of microwave treatment on peroxidase enzyme, bioactive compounds and color in tamarillo (Solanum betaceum Cav.) red varietyTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAgüero, M. 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Food Chemistry Advances, 100404. https://doi.org/10.1016/j.focha.2023.100404.EstudiantesGrupos comunitariosInvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85235/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1113619047.2023.pdf1113619047.2023.pdfTesis Maestría en Ingeniería Agroindustrialapplication/pdf891957https://repositorio.unal.edu.co/bitstream/unal/85235/2/1113619047.2023.pdf14532d32ceda26c4a60d8a533aa778a9MD52THUMBNAIL1113619047.2023.pdf.jpg1113619047.2023.pdf.jpgGenerated Thumbnailimage/jpeg5492https://repositorio.unal.edu.co/bitstream/unal/85235/3/1113619047.2023.pdf.jpg6fbed2cfd6e94f54b5f77e4df03888dbMD53unal/85235oai:repositorio.unal.edu.co:unal/852352024-08-19 23:11:32.671Repositorio Institucional Universidad Nacional de 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