Simultaneous saccharification and fermentation of cassava stems
This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained through the simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination and validation of the optimal...
- Autores:
-
Castaño Peláez, Hader Ivan
Reales Alfaro, Juan
Zapata Montoya, José Edgar
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2013
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/37831
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/37831
http://bdigital.unal.edu.co/27915/
- Palabra clave:
- Cassava Stems
Saccharification
Fermentation
Ethanol
Optimization
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Castaño Peláez, Hader Ivanf87f0376-a6f5-4291-a354-4dd596776d9d300Reales Alfaro, Juan22079bb0-a2f4-45a7-abea-a9a9aa4ba759300Zapata Montoya, José Edgarfb308c75-eae0-4ff0-ad56-92b95e93e1f53002019-06-28T02:03:06Z2019-06-28T02:03:06Z2013https://repositorio.unal.edu.co/handle/unal/37831http://bdigital.unal.edu.co/27915/This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained through the simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination and validation of the optimal conditions for and the evaluation of the process of ethanol production in a bioreactor. Alkaline-pretreated cassava stems were used as the substrate in a solid to liquid ratio of 1:10; the enzymatic complex Accellerase 1500 and the yeast Ethanol Red were evaluated at two levels at a temperature of 38° C and a pH of 4.0 in an Erlenmeyer fl ask. The following were evaluated as process controls: simultaneous saccharification and fermentation of non-pretreated stems and separate saccharifi cation and fermentation of pretreated stems. A regression analysis was conducted, and the resulting model was maximized using genetic algorithms. At the optimal conditions identified in an Erlenmeyer flask, the production of ethanol in a 5-liter bioreactor was subsequently evaluated. An experimental concentration of ethanol of 1.88±0.04% v/v (1.99% v/v simulated optimum) was obtained using an inoculum concentration of 1.59 g/L and an enzyme concentration of 13.3 FPU/g. This value was approximately four times the quantity of ethanol produced without pretreatment or by the separate saccharification and fermentation of pretreated cassava stems. The evaluation of the process in the bioreactor yielded an ethanol concentration 20% less than that reached in the Erlenmeyer flask.application/pdfspaUniversidad Nacional de Colombia Sede Medellínhttp://revistas.unal.edu.co/index.php/dyna/article/view/25824Universidad Nacional de Colombia Revistas electrónicas UN DynaDynaDyna; Vol. 80, núm. 180 (2013); 97-104 DYNA; Vol. 80, núm. 180 (2013); 97-104 2346-2183 0012-7353Castaño Peláez, Hader Ivan and Reales Alfaro, Juan and Zapata Montoya, José Edgar (2013) Simultaneous saccharification and fermentation of cassava stems. Dyna; Vol. 80, núm. 180 (2013); 97-104 DYNA; Vol. 80, núm. 180 (2013); 97-104 2346-2183 0012-7353 .Simultaneous saccharification and fermentation of cassava stemsArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTCassava StemsSaccharificationFermentationEthanolOptimizationORIGINAL25824-175608-1-PB.pdfapplication/pdf155402https://repositorio.unal.edu.co/bitstream/unal/37831/1/25824-175608-1-PB.pdff1a7f718c71fbfeaeb96e8dded418fcfMD5125824-90476-1-SP.docapplication/msword169984https://repositorio.unal.edu.co/bitstream/unal/37831/2/25824-90476-1-SP.doce9c329f338d8b60fca970dd901b64be8MD5225824-92345-1-SP.docxapplication/vnd.openxmlformats-officedocument.wordprocessingml.document109140https://repositorio.unal.edu.co/bitstream/unal/37831/3/25824-92345-1-SP.docx2f412eda30a61cd8d2dfefc66166d497MD53THUMBNAIL25824-175608-1-PB.pdf.jpg25824-175608-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8834https://repositorio.unal.edu.co/bitstream/unal/37831/4/25824-175608-1-PB.pdf.jpgeb49b0141009b5f532ce39b4c1353b88MD54unal/37831oai:repositorio.unal.edu.co:unal/378312023-01-17 23:08:58.004Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
Simultaneous saccharification and fermentation of cassava stems |
title |
Simultaneous saccharification and fermentation of cassava stems |
spellingShingle |
Simultaneous saccharification and fermentation of cassava stems Cassava Stems Saccharification Fermentation Ethanol Optimization |
title_short |
Simultaneous saccharification and fermentation of cassava stems |
title_full |
Simultaneous saccharification and fermentation of cassava stems |
title_fullStr |
Simultaneous saccharification and fermentation of cassava stems |
title_full_unstemmed |
Simultaneous saccharification and fermentation of cassava stems |
title_sort |
Simultaneous saccharification and fermentation of cassava stems |
dc.creator.fl_str_mv |
Castaño Peláez, Hader Ivan Reales Alfaro, Juan Zapata Montoya, José Edgar |
dc.contributor.author.spa.fl_str_mv |
Castaño Peláez, Hader Ivan Reales Alfaro, Juan Zapata Montoya, José Edgar |
dc.subject.proposal.spa.fl_str_mv |
Cassava Stems Saccharification Fermentation Ethanol Optimization |
topic |
Cassava Stems Saccharification Fermentation Ethanol Optimization |
description |
This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained through the simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination and validation of the optimal conditions for and the evaluation of the process of ethanol production in a bioreactor. Alkaline-pretreated cassava stems were used as the substrate in a solid to liquid ratio of 1:10; the enzymatic complex Accellerase 1500 and the yeast Ethanol Red were evaluated at two levels at a temperature of 38° C and a pH of 4.0 in an Erlenmeyer fl ask. The following were evaluated as process controls: simultaneous saccharification and fermentation of non-pretreated stems and separate saccharifi cation and fermentation of pretreated stems. A regression analysis was conducted, and the resulting model was maximized using genetic algorithms. At the optimal conditions identified in an Erlenmeyer flask, the production of ethanol in a 5-liter bioreactor was subsequently evaluated. An experimental concentration of ethanol of 1.88±0.04% v/v (1.99% v/v simulated optimum) was obtained using an inoculum concentration of 1.59 g/L and an enzyme concentration of 13.3 FPU/g. This value was approximately four times the quantity of ethanol produced without pretreatment or by the separate saccharification and fermentation of pretreated cassava stems. The evaluation of the process in the bioreactor yielded an ethanol concentration 20% less than that reached in the Erlenmeyer flask. |
publishDate |
2013 |
dc.date.issued.spa.fl_str_mv |
2013 |
dc.date.accessioned.spa.fl_str_mv |
2019-06-28T02:03:06Z |
dc.date.available.spa.fl_str_mv |
2019-06-28T02:03:06Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/37831 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/27915/ |
url |
https://repositorio.unal.edu.co/handle/unal/37831 http://bdigital.unal.edu.co/27915/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/dyna/article/view/25824 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Dyna Dyna |
dc.relation.ispartofseries.none.fl_str_mv |
Dyna; Vol. 80, núm. 180 (2013); 97-104 DYNA; Vol. 80, núm. 180 (2013); 97-104 2346-2183 0012-7353 |
dc.relation.references.spa.fl_str_mv |
Castaño Peláez, Hader Ivan and Reales Alfaro, Juan and Zapata Montoya, José Edgar (2013) Simultaneous saccharification and fermentation of cassava stems. Dyna; Vol. 80, núm. 180 (2013); 97-104 DYNA; Vol. 80, núm. 180 (2013); 97-104 2346-2183 0012-7353 . |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia Sede Medellín |
institution |
Universidad Nacional de Colombia |
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