Simultaneous saccharification and fermentation of cassava stems

This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained through the simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination and validation of the optimal...

Full description

Autores:
Castaño Peláez, Hader Ivan
Reales Alfaro, Juan
Zapata Montoya, José Edgar
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/37831
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/37831
http://bdigital.unal.edu.co/27915/
Palabra clave:
Cassava Stems
Saccharification
Fermentation
Ethanol
Optimization
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Castaño Peláez, Hader Ivanf87f0376-a6f5-4291-a354-4dd596776d9d300Reales Alfaro, Juan22079bb0-a2f4-45a7-abea-a9a9aa4ba759300Zapata Montoya, José Edgarfb308c75-eae0-4ff0-ad56-92b95e93e1f53002019-06-28T02:03:06Z2019-06-28T02:03:06Z2013https://repositorio.unal.edu.co/handle/unal/37831http://bdigital.unal.edu.co/27915/This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained through the simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination and validation of the optimal conditions for and the evaluation of the process of ethanol production in a bioreactor. Alkaline-pretreated cassava stems were used as the substrate in a solid to liquid ratio of 1:10; the enzymatic complex Accellerase 1500 and the yeast Ethanol Red were evaluated at two levels at a temperature of 38° C and a pH of 4.0 in an Erlenmeyer fl ask. The following were evaluated as process controls: simultaneous saccharification and fermentation of non-pretreated stems and separate saccharifi cation and fermentation of pretreated stems. A regression analysis was conducted, and the resulting model was maximized using genetic algorithms. At the optimal conditions identified in an Erlenmeyer flask, the production of ethanol in a 5-liter bioreactor was subsequently evaluated. An experimental concentration of ethanol of 1.88±0.04% v/v (1.99% v/v simulated optimum) was obtained using an inoculum concentration of 1.59 g/L and an enzyme concentration of 13.3 FPU/g. This value was approximately four times the quantity of ethanol produced without pretreatment or by the separate saccharification and fermentation of pretreated cassava stems. The evaluation of the process in the bioreactor yielded an ethanol concentration 20% less than that reached in the Erlenmeyer flask.application/pdfspaUniversidad Nacional de Colombia Sede Medellínhttp://revistas.unal.edu.co/index.php/dyna/article/view/25824Universidad Nacional de Colombia Revistas electrónicas UN DynaDynaDyna; Vol. 80, núm. 180 (2013); 97-104 DYNA; Vol. 80, núm. 180 (2013); 97-104 2346-2183 0012-7353Castaño Peláez, Hader Ivan and Reales Alfaro, Juan and Zapata Montoya, José Edgar (2013) Simultaneous saccharification and fermentation of cassava stems. Dyna; Vol. 80, núm. 180 (2013); 97-104 DYNA; Vol. 80, núm. 180 (2013); 97-104 2346-2183 0012-7353 .Simultaneous saccharification and fermentation of cassava stemsArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTCassava StemsSaccharificationFermentationEthanolOptimizationORIGINAL25824-175608-1-PB.pdfapplication/pdf155402https://repositorio.unal.edu.co/bitstream/unal/37831/1/25824-175608-1-PB.pdff1a7f718c71fbfeaeb96e8dded418fcfMD5125824-90476-1-SP.docapplication/msword169984https://repositorio.unal.edu.co/bitstream/unal/37831/2/25824-90476-1-SP.doce9c329f338d8b60fca970dd901b64be8MD5225824-92345-1-SP.docxapplication/vnd.openxmlformats-officedocument.wordprocessingml.document109140https://repositorio.unal.edu.co/bitstream/unal/37831/3/25824-92345-1-SP.docx2f412eda30a61cd8d2dfefc66166d497MD53THUMBNAIL25824-175608-1-PB.pdf.jpg25824-175608-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8834https://repositorio.unal.edu.co/bitstream/unal/37831/4/25824-175608-1-PB.pdf.jpgeb49b0141009b5f532ce39b4c1353b88MD54unal/37831oai:repositorio.unal.edu.co:unal/378312023-01-17 23:08:58.004Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Simultaneous saccharification and fermentation of cassava stems
title Simultaneous saccharification and fermentation of cassava stems
spellingShingle Simultaneous saccharification and fermentation of cassava stems
Cassava Stems
Saccharification
Fermentation
Ethanol
Optimization
title_short Simultaneous saccharification and fermentation of cassava stems
title_full Simultaneous saccharification and fermentation of cassava stems
title_fullStr Simultaneous saccharification and fermentation of cassava stems
title_full_unstemmed Simultaneous saccharification and fermentation of cassava stems
title_sort Simultaneous saccharification and fermentation of cassava stems
dc.creator.fl_str_mv Castaño Peláez, Hader Ivan
Reales Alfaro, Juan
Zapata Montoya, José Edgar
dc.contributor.author.spa.fl_str_mv Castaño Peláez, Hader Ivan
Reales Alfaro, Juan
Zapata Montoya, José Edgar
dc.subject.proposal.spa.fl_str_mv Cassava Stems
Saccharification
Fermentation
Ethanol
Optimization
topic Cassava Stems
Saccharification
Fermentation
Ethanol
Optimization
description This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained through the simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination and validation of the optimal conditions for and the evaluation of the process of ethanol production in a bioreactor. Alkaline-pretreated cassava stems were used as the substrate in a solid to liquid ratio of 1:10; the enzymatic complex Accellerase 1500 and the yeast Ethanol Red were evaluated at two levels at a temperature of 38° C and a pH of 4.0 in an Erlenmeyer fl ask. The following were evaluated as process controls: simultaneous saccharification and fermentation of non-pretreated stems and separate saccharifi cation and fermentation of pretreated stems. A regression analysis was conducted, and the resulting model was maximized using genetic algorithms. At the optimal conditions identified in an Erlenmeyer flask, the production of ethanol in a 5-liter bioreactor was subsequently evaluated. An experimental concentration of ethanol of 1.88±0.04% v/v (1.99% v/v simulated optimum) was obtained using an inoculum concentration of 1.59 g/L and an enzyme concentration of 13.3 FPU/g. This value was approximately four times the quantity of ethanol produced without pretreatment or by the separate saccharification and fermentation of pretreated cassava stems. The evaluation of the process in the bioreactor yielded an ethanol concentration 20% less than that reached in the Erlenmeyer flask.
publishDate 2013
dc.date.issued.spa.fl_str_mv 2013
dc.date.accessioned.spa.fl_str_mv 2019-06-28T02:03:06Z
dc.date.available.spa.fl_str_mv 2019-06-28T02:03:06Z
dc.type.spa.fl_str_mv Artículo de revista
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url https://repositorio.unal.edu.co/handle/unal/37831
http://bdigital.unal.edu.co/27915/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/dyna/article/view/25824
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Dyna
Dyna
dc.relation.ispartofseries.none.fl_str_mv Dyna; Vol. 80, núm. 180 (2013); 97-104 DYNA; Vol. 80, núm. 180 (2013); 97-104 2346-2183 0012-7353
dc.relation.references.spa.fl_str_mv Castaño Peláez, Hader Ivan and Reales Alfaro, Juan and Zapata Montoya, José Edgar (2013) Simultaneous saccharification and fermentation of cassava stems. Dyna; Vol. 80, núm. 180 (2013); 97-104 DYNA; Vol. 80, núm. 180 (2013); 97-104 2346-2183 0012-7353 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
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dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia Sede Medellín
institution Universidad Nacional de Colombia
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