Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos

ilustraciones, diagramas

Autores:: Morera Bedoya, German Darío

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos
dc.title.translated.eng.fl_str_mv	Biochemical study of the functional potential of two strains of Ganoderma lucidum (Reishi) cultivated in colombia for their application in the food industry
title	Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos
spellingShingle	Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Valor nutritivo nutritive value Industria alimentaria food industry Ganoderma lucidum Ganoderma lucidum Ganoderma AGEs aglutinación Compuestos bioactivos Triterpenos Lectina Ganoderma Bioactive compounds Triterpenes AGEs Lectin Agglutination
title_short	Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos
title_full	Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos
title_fullStr	Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos
title_full_unstemmed	Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos
title_sort	Estudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentos
dc.creator.fl_str_mv	Morera Bedoya, German Darío
dc.contributor.advisor.spa.fl_str_mv	Ardila Barrantes, Harold Duban Chegwin Angarita, Carolina
dc.contributor.author.spa.fl_str_mv	Morera Bedoya, German Darío
dc.contributor.researchgroup.spa.fl_str_mv	Estudio de Actividades Metabolicas Vegetales Química de Hongos Macromicetos Colombianos
dc.contributor.orcid.spa.fl_str_mv	MORERA BEDOYA, GERMÁN DARIO [0000-0003-0633-0476]
dc.contributor.cvlac.spa.fl_str_mv	MORERA BEDOYA, GERMÁN DARIO
dc.subject.ddc.spa.fl_str_mv	630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
topic	630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Valor nutritivo nutritive value Industria alimentaria food industry Ganoderma lucidum Ganoderma lucidum Ganoderma AGEs aglutinación Compuestos bioactivos Triterpenos Lectina Ganoderma Bioactive compounds Triterpenes AGEs Lectin Agglutination
dc.subject.agrovoc.none.fl_str_mv	Valor nutritivo nutritive value Industria alimentaria food industry Ganoderma lucidum Ganoderma lucidum
dc.subject.proposal.spa.fl_str_mv	Ganoderma AGEs aglutinación Compuestos bioactivos Triterpenos Lectina
dc.subject.proposal.eng.fl_str_mv	Ganoderma Bioactive compounds Triterpenes AGEs Lectin Agglutination
description	ilustraciones, diagramas
publishDate	2023
dc.date.issued.none.fl_str_mv	2023-08-01
dc.date.accessioned.none.fl_str_mv	2024-01-18T20:16:17Z
dc.date.available.none.fl_str_mv	2024-01-18T20:16:17Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/85371
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/85371 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.indexed.spa.fl_str_mv	Agrosavia Agrovoc
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dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ardila Barrantes, Harold Duban93a2ba429f45d3423831e7f452508cbb600Chegwin Angarita, Carolina04e1dc7774dc51a9525d964cb6addc0aMorera Bedoya, German Daríofb7e316637da877d24a0153ea543f2ebEstudio de Actividades Metabolicas VegetalesQuímica de Hongos Macromicetos ColombianosMORERA BEDOYA, GERMÁN DARIO [0000-0003-0633-0476]MORERA BEDOYA, GERMÁN DARIO2024-01-18T20:16:17Z2024-01-18T20:16:17Z2023-08-01https://repositorio.unal.edu.co/handle/unal/85371Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasEl hongo macromiceto Ganoderma lucidum, es una fuente de compuestos bioactivos con actividades biológicas científicamente comprobadas. A pesar de esto, hay pocos estudios enfocados a estudiar el potencial bioquímico de las cepas de este hongo comercializadas en nuestro país. Es por ello por lo que en esta investigación se presentan los resultados del estudio de la capacidad antioxidante y la actividad inhibitoria de AGEs in vitro, así como la presencia de lectinas con actividad aglutinante, de dos cepas de este hongo cultivadas en Colombia de manera tradicional a escala industrial (EUA y BEL). Para ello se realizaron extractos empleando solventes de diferente polaridad y se evaluaron por métodos espectrofotométricos, la capacidad antioxidante y la inhibición de la formación de productos avanzados de glicosilación AGEs. Este análisis se complementó con un estudio dirigido a las potenciales moléculas bioactivas, usando determinación de metabolitos por métodos espectrofotométricos, perfilado cromatográfico (HPLC-DAD y CG-MS) y análisis estadísticos multivariados (PLS-DA). Finalmente, se evaluó en un modelo in vitro la presencia de lectinas midiendo la aglutinación de eritrocitos a partir de extractos proteicos de las dos cepas en estudio. Se encontró que los extractos etanólicos y en diclorometano de las dos cepas, presentan actividad antioxidante e inhibitoria de AGE. Sin embargo, la cepa BEL presentó los mayores niveles de dichas actividades; el análisis cromatográfico y estadístico permitió encontrar qué, diferentes metabolitos del tipo triterpenos, están relacionados con el potencial antioxidante e inhibitorio de AGEs. Por otro lado, se encontró que en esta misma cepa se presenta importante actividad antiaglutinante; la purificación y caracterización de las lectinas presentes permitieron determinar que en la cepa BEL, se presentan lectinas con propiedades bioquímicas que le otorgan potencial para ser usadas en la industria de alimentos. El presente estudio contribuye al conocimiento de la composición bioquímica del hongo G. lucidum y confirma su actividad biológica in vitro en el micelio producido en Colombia. (Texto tomado de la fuente).The mushroom fungus Ganoderma lucidum is a source of bioactive compounds with scientifically proven biological activities. Despite this, there are few studies focused on studying the biochemical potential of the strains of this fungus commercialized in our country. For this reason, this research presents the results of the study of the antioxidant capacity and the inhibitory activity of AGEs in vitro, as well as the presence of lectins with binding activity, of two strains of this fungus traditionally cultivated in Colombia on an industrial scale (EUA and BEL). For this purpose, extracts were made, using solvents of different polarity, and were evaluated by spectrophotometric methods, the antioxidant capacity and the inhibition of the formation of advanced AGEs glycosylation products. This analysis was complemented with a study aimed at potential bioactive molecules, using metabolite determination by spectrophotometric methods, chromatographic profiling (HPLC-DAD and GC-MS) and multivariate statistical analysis (PLS-DA). Finally, the presence of lectins was evaluated in an in vitro model by measuring the agglutination of erythrocytes from protein extracts of the two strains under study. It was found that the ethanolic and dichloromethane extracts of the two strains showed antioxidant and AGE inhibitory activity. However, the BEL strain presented the highest levels of these activities; the chromatographic and statistical analysis allowed finding that different metabolites of the triterpene type are related to the antioxidant and AGE inhibitory potential. On the other hand, it was found that this strain has an important antiagglutinating activity; the purification and characterization of the lectins present allowed determining that the BEL strain has lectins with biochemical properties that give it the potential to be used in the industry. The present study contributes to the knowledge of the biochemical composition of the fungus G. lucidum and confirms it is in vitro biological activity in the mycelium produced in Colombia.MaestríaMagíster en Ciencias - Bioquímicaxi, 131 páginasapplication/pdfspa630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materialesValor nutritivonutritive valueIndustria alimentariafood industryGanoderma lucidumGanoderma lucidumGanodermaAGEsaglutinaciónCompuestos bioactivosTriterpenosLectinaGanodermaBioactive compoundsTriterpenesAGEsLectinAgglutinationEstudio bioquímico del potencial funcional de dos cepas de Ganoderma lucidum (Reishi) cultivadas en Colombia para su aplicación en la industria de alimentosBiochemical study of the functional potential of two strains of Ganoderma lucidum (Reishi) cultivated in colombia for their application in the food industryTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBogotá - Ciencias - Maestría en Ciencias - BioquímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede BogotáColombiahttp://vocab.getty.edu/page/tgn/1000050AgrosaviaAgrovocXia Q, Zhang H, Sun X, Zhao H, Wu L, Zhu D, et al.Kong M, Yao Y, Zhang H. 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Vol. 34, Rev Cubana Farm. 2000.EstudiantesGrupos comunitariosInvestigadoresPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85371/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1072661688.2023.pdf1072661688.2023.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf2911274https://repositorio.unal.edu.co/bitstream/unal/85371/2/1072661688.2023.pdf15426bd20245b971358fb07ce30bc0fcMD52THUMBNAIL1072661688.2023.pdf.jpg1072661688.2023.pdf.jpgGenerated Thumbnailimage/jpeg6431https://repositorio.unal.edu.co/bitstream/unal/85371/3/1072661688.2023.pdf.jpg392621dd271d5c27528ec5fa614164e2MD53unal/85371oai:repositorio.unal.edu.co:unal/853712024-08-21 23:12:52.941Repositorio Institucional Universidad Nacional de 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