Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp

El café es uno de los productos insignia de la economía colombiana, contribuyendo en 2021 con el 1.0% al PIB nacional y el 15% al PIB agrícola. Sin embargo, su cadena productiva deja tras de sí diferentes residuos que generan un impacto ambiental negativo en las áreas rurales del país, uno de estos...

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Autores:: Rendón Muñoz, Yazmín

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp
dc.title.translated.eng.fl_str_mv	Coffee mucilage fermentation to obtain bacterial cellulose using wild strains of Komagataeibacter spp
title	Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp
spellingShingle	Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp 660 - Ingeniería química 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Aprovechamiento de residuos Mucilago Celulosa Residuos agrícolas Aprovechamiento de residuos Cadena productiva de café Celulosa bacteriana Fermentación Residuo agrícola Waste management Coffee supply chain Bacterial cellulose Fermentation Alternative medium Agricultural waste
title_short	Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp
title_full	Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp
title_fullStr	Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp
title_full_unstemmed	Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp
title_sort	Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp
dc.creator.fl_str_mv	Rendón Muñoz, Yazmín
dc.contributor.advisor.none.fl_str_mv	Cadena Chamorro, Edith Marleny Santa Marín, Juan Felipe
dc.contributor.author.none.fl_str_mv	Rendón Muñoz, Yazmín
dc.contributor.researchgroup.spa.fl_str_mv	Ingeniería Agrícola
dc.contributor.orcid.spa.fl_str_mv	Rendon-Munoz, Yazmin [0000-0003-4417-6772]
dc.contributor.googlescholar.spa.fl_str_mv	https://scholar.google.es/citations?user=S43z40cAAAAJ&hl=es
dc.subject.ddc.spa.fl_str_mv	660 - Ingeniería química 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
topic	660 - Ingeniería química 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Aprovechamiento de residuos Mucilago Celulosa Residuos agrícolas Aprovechamiento de residuos Cadena productiva de café Celulosa bacteriana Fermentación Residuo agrícola Waste management Coffee supply chain Bacterial cellulose Fermentation Alternative medium Agricultural waste
dc.subject.lemb.none.fl_str_mv	Aprovechamiento de residuos Mucilago Celulosa Residuos agrícolas
dc.subject.proposal.spa.fl_str_mv	Aprovechamiento de residuos Cadena productiva de café Celulosa bacteriana Fermentación Residuo agrícola
dc.subject.proposal.eng.fl_str_mv	Waste management Coffee supply chain Bacterial cellulose Fermentation Alternative medium Agricultural waste
description	El café es uno de los productos insignia de la economía colombiana, contribuyendo en 2021 con el 1.0% al PIB nacional y el 15% al PIB agrícola. Sin embargo, su cadena productiva deja tras de sí diferentes residuos que generan un impacto ambiental negativo en las áreas rurales del país, uno de estos residuos es el mucílago que se genera en grandes cantidades y afecta principalmente fuentes hídricas. En aras de buscar usos alternativos para este residuo, el presente trabajo evaluó su potencial como medio de cultivo en la producción de celulosa bacteriana, polímero de gran valor utilizado en diferentes áreas de la industria. En el desarrollo experimental se utilizó mucílago de café obtenido a través de dos métodos de extracción, fermentación natural y desmucilaginado mecánico, y como medio de cultivo control se usó la formulación HS. Cinco (5) microorganismos productores de celulosa bacteriana se aislaron a partir de vinagre casero de panela, de los cuales el mejor productor de celulosa fue clasificado dentro de la especie Komagataeibacter intermedius. La fermentación con este aislado se llevó a cabo en un sistema batch conservando una relación área superficial/volumen de 0.70 cm-1. De igual forma, se evaluó el efecto de variables operaciones como temperatura e inyección de aire en la generación de celulosa con mucílago de café como medio de cultivo. La mayor producción de celulosa bacteriana se obtuvo con mucílago de fermentación natural (4.20±0.01 g/L), seguido por mucílago de desmucilaginado mecánico (1.69±0.04 g/L) y el medio HS (0.77±0.01 g/L) después de 10 d de fermentación. El seguimiento a la cinética del proceso reveló un decrecimiento abrupto en el pH y oxígeno disuelto en los primeros dos días de fermentación, dando indicios sobre el comportamiento de la bacteria en estos medios. La modificación de variables operacionales como temperatura y adaptación de un sistema de aireación sobre el proceso fermentativo reveló que la temperatura óptima para la producción de celulosa bacteriana es 35 °C y que los sistemas con inyección de aire propician un incremento del 22% en la generación de celulosa. La caracterización de las matrices celulósicas obtenidas a partir del mucílago de café reveló que estas presentan una alta capacidad de retención de agua (>150 gH2O/gcelulosa), alta estabilidad térmica determinada por temperaturas de degradación (>360 °C), resistencia mecánica con un alto módulo de Young (>17 GPa) e índice de cristalinidad (>85%). Lo mencionado anteriormente demuestra que el mucílago puede ser catalogado como un medio cultivo alternativo idóneo en la producción de celulosa bacteriana, ya que genera altos rendimientos y permite obtener celulosas con características promisorias para ser usadas en diferentes aplicaciones. (Texto tomado de la fuente)
publishDate	2023
dc.date.accessioned.none.fl_str_mv	2023-07-31T19:25:09Z
dc.date.available.none.fl_str_mv	2023-07-31T19:25:09Z
dc.date.issued.none.fl_str_mv	2023-07-29
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/84374
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/84374 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.indexed.spa.fl_str_mv	LaReferencia
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cadena Chamorro, Edith Marleny3b754ee7ed16a28246dcc1bb8bb386ab600Santa Marín, Juan Felipe30f321423032a600a1187f1d8ce07534600Rendón Muñoz, Yazmín4b4560046a15d489be6331698d42782cIngeniería AgrícolaRendon-Munoz, Yazmin [0000-0003-4417-6772]https://scholar.google.es/citations?user=S43z40cAAAAJ&hl=es2023-07-31T19:25:09Z2023-07-31T19:25:09Z2023-07-29https://repositorio.unal.edu.co/handle/unal/84374Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/El café es uno de los productos insignia de la economía colombiana, contribuyendo en 2021 con el 1.0% al PIB nacional y el 15% al PIB agrícola. Sin embargo, su cadena productiva deja tras de sí diferentes residuos que generan un impacto ambiental negativo en las áreas rurales del país, uno de estos residuos es el mucílago que se genera en grandes cantidades y afecta principalmente fuentes hídricas. En aras de buscar usos alternativos para este residuo, el presente trabajo evaluó su potencial como medio de cultivo en la producción de celulosa bacteriana, polímero de gran valor utilizado en diferentes áreas de la industria. En el desarrollo experimental se utilizó mucílago de café obtenido a través de dos métodos de extracción, fermentación natural y desmucilaginado mecánico, y como medio de cultivo control se usó la formulación HS. Cinco (5) microorganismos productores de celulosa bacteriana se aislaron a partir de vinagre casero de panela, de los cuales el mejor productor de celulosa fue clasificado dentro de la especie Komagataeibacter intermedius. La fermentación con este aislado se llevó a cabo en un sistema batch conservando una relación área superficial/volumen de 0.70 cm-1. De igual forma, se evaluó el efecto de variables operaciones como temperatura e inyección de aire en la generación de celulosa con mucílago de café como medio de cultivo. La mayor producción de celulosa bacteriana se obtuvo con mucílago de fermentación natural (4.20±0.01 g/L), seguido por mucílago de desmucilaginado mecánico (1.69±0.04 g/L) y el medio HS (0.77±0.01 g/L) después de 10 d de fermentación. El seguimiento a la cinética del proceso reveló un decrecimiento abrupto en el pH y oxígeno disuelto en los primeros dos días de fermentación, dando indicios sobre el comportamiento de la bacteria en estos medios. La modificación de variables operacionales como temperatura y adaptación de un sistema de aireación sobre el proceso fermentativo reveló que la temperatura óptima para la producción de celulosa bacteriana es 35 °C y que los sistemas con inyección de aire propician un incremento del 22% en la generación de celulosa. La caracterización de las matrices celulósicas obtenidas a partir del mucílago de café reveló que estas presentan una alta capacidad de retención de agua (>150 gH2O/gcelulosa), alta estabilidad térmica determinada por temperaturas de degradación (>360 °C), resistencia mecánica con un alto módulo de Young (>17 GPa) e índice de cristalinidad (>85%). Lo mencionado anteriormente demuestra que el mucílago puede ser catalogado como un medio cultivo alternativo idóneo en la producción de celulosa bacteriana, ya que genera altos rendimientos y permite obtener celulosas con características promisorias para ser usadas en diferentes aplicaciones. (Texto tomado de la fuente)Coffee is one of the Colombian economy-flagship products, contributing to 1.0% of the national GDP and 15% of the agricultural GDP in 2021. However, coffee supply chain leaves behind different residues that generate a negative environmental impact in the Colombian rural areas. One of these residues is the mucilage, which is generated in large quantities and mainly affects water sources. In order to find alternative uses for this residue, the present work evaluated mucilage potential as a culture medium in the production of bacterial cellulose, a high-value polymer used in different industrial areas. Coffee mucilage obtained by two extraction methods, natural fermentation and mechanical removal, was used throughout this research. HS formulation was also used as control culture medium. Five (5) bacterial cellulose-producing microorganisms were isolated from homemade raw-sugar-cane vinegar, being the best cellulose producer classified into the species Komagataeibacter intermedius. The fermentative process was carried out in a batch system with this isolate, maintaining a surface area/volume ratio of 0.70 cm-1 for 10 d. Modifications in operational variables, such as temperature and aeration system, along with coffee mucilage were also evaluated on cellulose generation. The highest yield of bacterial cellulose was obtained with natural fermentation mucilage (4.20±0.01 g/L), followed by mechanical removal mucilage (1.69±0.04 g/L) and HS medium (0.77±0.01 g/L) after 10 d. A pronounced decrease in pH and dissolved oxygen in the first two days was observed, giving hints about the bacterium behaviour in these media. Changes in operational variables revealed that the optimal temperature to produce bacterial cellulose is 35 °C, and air-input promotes a 22% increase in cellulose generation. The characterization of the cellulosic matrices obtained from coffee mucilage revealed that they have a high-water retention capacity (>150 gH2O/gcellulose), high thermal stability (>360 °C), mechanical resistance with a high Young modulus' (>17 GPa), and high crystallinity index (>85%). The outcomes demonstrate that mucilage can be recognized as a suitable alternative medium since it generates high cellulose yields with promising characteristics.MaestríaMagíster en Ciencias - BiotecnologíaAprovechamiento de residuos agrícolasMateriales sustentablesÁrea curricular Biotecnología148 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín660 - Ingeniería química630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materialesAprovechamiento de residuosMucilagoCelulosaResiduos agrícolasAprovechamiento de residuosCadena productiva de caféCelulosa bacterianaFermentaciónResiduo agrícolaWaste managementCoffee supply chainBacterial celluloseFermentationAlternative mediumAgricultural wasteFermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter sppCoffee mucilage fermentation to obtain bacterial cellulose using wild strains of Komagataeibacter sppTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMLaReferenciaAbidi, W., Torres-Sánchez, L., Siroy, A., & Krasteva, P. 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Journal of Industrial Microbiology & Biotechnology, 34(7), 483-489. https://doi.org/10.1007/s10295-007-0218-4Valorisation of wastes from coffee supply chain in Colombian and UK to develop novel productsAlianza estratégica para la valorización de residuos provenientes del beneficio de café para la obtención de celulosa bacteriana y su aplicación en matrices poliméricasFondo Nacional de Financiamiento para La Ciencia, La Tecnología y La Innovación “FRANCISCO JOSÉ DE CALDAS”Ministerio de Ciencia y Tecnología de Colombia (MinCiencias)Universidad Nacional De ColombiaEstudiantesInvestigadoresMaestrosMedios de comunicaciónPúblico generalLICENSElicense.txtlicense.txttext/plain; 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Fermentación del mucílago de café para la obtención de celulosa bacteriana con aislados nativos de Komagataeibacter spp

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