Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening

The araza is a fruit native of the Amazon region, like other fruits of this region has some antioxidant components. This work was carried out the classification of the fruits of araza in four different states using as the index of maturity, then phenolic compounds were extracted using mixtures of so...

Full description

Autores:
Cuellar, Fabio A.
Ariza Cubillos, Edna Alejandra
Anzola, Cecilia
Restrepo, Patricia
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/49804
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/49804
http://bdigital.unal.edu.co/43293/
Palabra clave:
Arazá
capacidad antioxidante
ABTS
DPPH
FRAP
decoloración del β-caroteno
madurez
Araza
antioxidant capacity
ABTS
DPPH
FRAP
β-carotene bleaching
ripening
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_ccc710816d23dfbf683533b5105a91df
oai_identifier_str oai:repositorio.unal.edu.co:unal/49804
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
title Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
spellingShingle Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
Arazá
capacidad antioxidante
ABTS
DPPH
FRAP
decoloración del β-caroteno
madurez
Araza
antioxidant capacity
ABTS
DPPH
FRAP
β-carotene bleaching
ripening
title_short Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
title_full Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
title_fullStr Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
title_full_unstemmed Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
title_sort Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
dc.creator.fl_str_mv Cuellar, Fabio A.
Ariza Cubillos, Edna Alejandra
Anzola, Cecilia
Restrepo, Patricia
dc.contributor.author.spa.fl_str_mv Cuellar, Fabio A.
Ariza Cubillos, Edna Alejandra
Anzola, Cecilia
Restrepo, Patricia
dc.subject.proposal.spa.fl_str_mv Arazá
capacidad antioxidante
ABTS
DPPH
FRAP
decoloración del β-caroteno
madurez
Araza
antioxidant capacity
ABTS
DPPH
FRAP
β-carotene bleaching
ripening
topic Arazá
capacidad antioxidante
ABTS
DPPH
FRAP
decoloración del β-caroteno
madurez
Araza
antioxidant capacity
ABTS
DPPH
FRAP
β-carotene bleaching
ripening
description The araza is a fruit native of the Amazon region, like other fruits of this region has some antioxidant components. This work was carried out the classification of the fruits of araza in four different states using as the index of maturity, then phenolic compounds were extracted using mixtures of solvents (methanol, acetone, water) and your content was determined by the spectrophotometric method Folin-Ciocalteu in the epicarp and mesocarp. Additionally, partially identified phenolic compounds by reversed phase HPLC with diode array, finding the phenolic acids such as chlorogenic, gallic and caffeic, such as phenols responsible for the majority of araza antioxidant activity. It was noted that araza mesocarp polyphenol content was higher in the green (1200 mg/1000g fruto BH) state followed by mature (1200 mg/1000g fruto BH), pinton (905 mg/1000g fruto BH) and over mature (550 mg/1000g fruto BH) while the epicarp was greater in states mature (170 mg/1000g fruto BH) and green (295 mg/1000g fruto BH) followed of the over-mature (50 mg/1000g fruto BH) and pinton (100 mg/1000g fruto BH) states. The antioxidant capacity was determined by three hydrophilic methods (ABTS, DPPH and FRAP) in the mesocarp found that the antioxidant capacity was higher in the green state followed by mature), pinton  and over mature while the epicarp was higher in mature, green, pinton) and over-mature state. We also determined the antioxidant capacity by a lipophilic method (b-carotene bleaching) found that in the mesocarp was higher in states followed by mature (110 mg/1000g fruto BH), pinton (90 mg/1000g fruto BH) and  green (26 mg/1000g fruto BH) while in the epicarp was higher in the over mature stage (50 mg/1000g fruto BH)followed by greeen states (45 mg/1000g fruto BH), mature (55 mg/1000g fruto BH)and  sobre mature  (25 mg/1000g fruto BH). Finally we conclude that the mature and green had the highest antioxidant capacity, phenolic compounds being present in the epicarp and mesocarp were the major contributors.
publishDate 2013
dc.date.issued.spa.fl_str_mv 2013
dc.date.accessioned.spa.fl_str_mv 2019-06-29T09:20:03Z
dc.date.available.spa.fl_str_mv 2019-06-29T09:20:03Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/49804
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/43293/
url https://repositorio.unal.edu.co/handle/unal/49804
http://bdigital.unal.edu.co/43293/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/rcolquim/article/view/45885
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Química
Revista Colombiana de Química
dc.relation.ispartofseries.none.fl_str_mv Revista Colombiana de Química; Vol. 42, núm. 2 (2013): Versión preliminar; 213-235 2357-3791 0120-2804
dc.relation.references.spa.fl_str_mv Cuellar, Fabio A. and Ariza Cubillos, Edna Alejandra and Anzola, Cecilia and Restrepo, Patricia (2013) Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening. Revista Colombiana de Química; Vol. 42, núm. 2 (2013): Versión preliminar; 213-235 2357-3791 0120-2804 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/49804/1/45885-222568-1-SM.pdf
https://repositorio.unal.edu.co/bitstream/unal/49804/2/45885-222568-1-SM.pdf.jpg
bitstream.checksum.fl_str_mv 715a33fb10043f37e96dff156d0822d4
9704d556b48b3047ae5a0eaab0aaf45e
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv repositorio_nal@unal.edu.co
_version_ 1814089736966373376
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cuellar, Fabio A.b06a6f39-be91-4f01-950f-eb900153c677300Ariza Cubillos, Edna Alejandra6e18e2a1-6c13-4534-9414-d32657e2cc8f300Anzola, Cecilia4ed9e469-2044-44fc-9af0-3d91063a5a91300Restrepo, Patricia2c8b5188-b632-48e6-8f8a-0c24bcccbf953002019-06-29T09:20:03Z2019-06-29T09:20:03Z2013https://repositorio.unal.edu.co/handle/unal/49804http://bdigital.unal.edu.co/43293/The araza is a fruit native of the Amazon region, like other fruits of this region has some antioxidant components. This work was carried out the classification of the fruits of araza in four different states using as the index of maturity, then phenolic compounds were extracted using mixtures of solvents (methanol, acetone, water) and your content was determined by the spectrophotometric method Folin-Ciocalteu in the epicarp and mesocarp. Additionally, partially identified phenolic compounds by reversed phase HPLC with diode array, finding the phenolic acids such as chlorogenic, gallic and caffeic, such as phenols responsible for the majority of araza antioxidant activity. It was noted that araza mesocarp polyphenol content was higher in the green (1200 mg/1000g fruto BH) state followed by mature (1200 mg/1000g fruto BH), pinton (905 mg/1000g fruto BH) and over mature (550 mg/1000g fruto BH) while the epicarp was greater in states mature (170 mg/1000g fruto BH) and green (295 mg/1000g fruto BH) followed of the over-mature (50 mg/1000g fruto BH) and pinton (100 mg/1000g fruto BH) states. The antioxidant capacity was determined by three hydrophilic methods (ABTS, DPPH and FRAP) in the mesocarp found that the antioxidant capacity was higher in the green state followed by mature), pinton  and over mature while the epicarp was higher in mature, green, pinton) and over-mature state. We also determined the antioxidant capacity by a lipophilic method (b-carotene bleaching) found that in the mesocarp was higher in states followed by mature (110 mg/1000g fruto BH), pinton (90 mg/1000g fruto BH) and  green (26 mg/1000g fruto BH) while in the epicarp was higher in the over mature stage (50 mg/1000g fruto BH)followed by greeen states (45 mg/1000g fruto BH), mature (55 mg/1000g fruto BH)and  sobre mature  (25 mg/1000g fruto BH). Finally we conclude that the mature and green had the highest antioxidant capacity, phenolic compounds being present in the epicarp and mesocarp were the major contributors.El arazá es un fruto amazónico con buena capacidad antioxidante. En este trabajo se realizó, a partir del epicarpio y mesocarpio de la fruta en cuatro estados de madurez (verde, pintón, maduro y sobremaduro), la extracción de compuestos fenólicos (utilizando como solvente una mezcla de agua:acetona, ( inicial 50:50 y posterior de 70:30)) y su análisis (método colorimétrico de Folin-Ciocalteu), y se identificaron parcialmente por HPLC en fase reversa, con arreglo de diodos, encontrando  los ácidos clorogénico, gálico y caféico   como  fenoles mayoritarios  responsables de la actividad antioxidante. El contenido de polifenoles en el mesocarpio  es mayor en el estado verde (1200mg/1000g fruto BH) seguido del  maduro (1100mg/1000g fruto BH), pintón (905 mg/1000g fruto BH) y sobremaduro (550mg/1000g fruto BH), mientras que en el epicarpio es mayor en  estados maduro (170 mg/1000g fruto BH) y verde (295mg/1000g fruto BH) seguidos del pintón (100 mg/1000g fruto BH) y sobremaduro (50 mg/1000g fruto BH). La capacidad antioxidante fue determinada por  métodos hidrofílicos (ABTS, DPPH y FRAP) encontrando que en el mesocarpio la capacidad antioxidante medida por todos los métodos  es mayor en  estado verde seguido del  maduro, pintón y sobremaduro mientras en el epicarpio es mayor en  maduro y verde seguido del pintón y sobremaduro. Se determinó la capacidad antioxidante por un método lipofílico (decoloración del β-caroteno) encontrando que en el mesocarpio es mayor en estados maduro (110 mg/1000g fruto BH) y pintón (90 mg/1000g fruto BH) seguido del sobremaduro ( 75mg/1000g fruto BH) y verde (26mg/1000g fruto BH) y en el epicarpio es mayor en sobremaduro (50 mg/1000g fruto BH) seguido del verde (45 mg/1000g fruto BH), maduro (55 mg/1000g fruto BH) y pintón (25 mg/1000g fruto BH). Finalmente se concluye que el fruto de arazá en los estados verde y maduro presenta la mayor capacidad antioxidante, siendo los ácidos  fenólicos los principales contribuyentes.application/pdfspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/rcolquim/article/view/45885Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de QuímicaRevista Colombiana de QuímicaRevista Colombiana de Química; Vol. 42, núm. 2 (2013): Versión preliminar; 213-235 2357-3791 0120-2804Cuellar, Fabio A. and Ariza Cubillos, Edna Alejandra and Anzola, Cecilia and Restrepo, Patricia (2013) Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening. Revista Colombiana de Química; Vol. 42, núm. 2 (2013): Versión preliminar; 213-235 2357-3791 0120-2804 .Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripeningArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTArazácapacidad antioxidanteABTSDPPHFRAPdecoloración del β-carotenomadurezArazaantioxidant capacityABTSDPPHFRAPβ-carotene bleachingripeningORIGINAL45885-222568-1-SM.pdfapplication/pdf871706https://repositorio.unal.edu.co/bitstream/unal/49804/1/45885-222568-1-SM.pdf715a33fb10043f37e96dff156d0822d4MD51THUMBNAIL45885-222568-1-SM.pdf.jpg45885-222568-1-SM.pdf.jpgGenerated Thumbnailimage/jpeg7604https://repositorio.unal.edu.co/bitstream/unal/49804/2/45885-222568-1-SM.pdf.jpg9704d556b48b3047ae5a0eaab0aaf45eMD52unal/49804oai:repositorio.unal.edu.co:unal/498042022-12-17 23:04:09.529Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co