Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening
The araza is a fruit native of the Amazon region, like other fruits of this region has some antioxidant components. This work was carried out the classification of the fruits of araza in four different states using as the index of maturity, then phenolic compounds were extracted using mixtures of so...
- Autores:
-
Cuellar, Fabio A.
Ariza Cubillos, Edna Alejandra
Anzola, Cecilia
Restrepo, Patricia
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2013
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/49804
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/49804
http://bdigital.unal.edu.co/43293/
- Palabra clave:
- Arazá
capacidad antioxidante
ABTS
DPPH
FRAP
decoloración del β-caroteno
madurez
Araza
antioxidant capacity
ABTS
DPPH
FRAP
β-carotene bleaching
ripening
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
id |
UNACIONAL2_ccc710816d23dfbf683533b5105a91df |
---|---|
oai_identifier_str |
oai:repositorio.unal.edu.co:unal/49804 |
network_acronym_str |
UNACIONAL2 |
network_name_str |
Universidad Nacional de Colombia |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening |
title |
Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening |
spellingShingle |
Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening Arazá capacidad antioxidante ABTS DPPH FRAP decoloración del β-caroteno madurez Araza antioxidant capacity ABTS DPPH FRAP β-carotene bleaching ripening |
title_short |
Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening |
title_full |
Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening |
title_fullStr |
Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening |
title_full_unstemmed |
Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening |
title_sort |
Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening |
dc.creator.fl_str_mv |
Cuellar, Fabio A. Ariza Cubillos, Edna Alejandra Anzola, Cecilia Restrepo, Patricia |
dc.contributor.author.spa.fl_str_mv |
Cuellar, Fabio A. Ariza Cubillos, Edna Alejandra Anzola, Cecilia Restrepo, Patricia |
dc.subject.proposal.spa.fl_str_mv |
Arazá capacidad antioxidante ABTS DPPH FRAP decoloración del β-caroteno madurez Araza antioxidant capacity ABTS DPPH FRAP β-carotene bleaching ripening |
topic |
Arazá capacidad antioxidante ABTS DPPH FRAP decoloración del β-caroteno madurez Araza antioxidant capacity ABTS DPPH FRAP β-carotene bleaching ripening |
description |
The araza is a fruit native of the Amazon region, like other fruits of this region has some antioxidant components. This work was carried out the classification of the fruits of araza in four different states using as the index of maturity, then phenolic compounds were extracted using mixtures of solvents (methanol, acetone, water) and your content was determined by the spectrophotometric method Folin-Ciocalteu in the epicarp and mesocarp. Additionally, partially identified phenolic compounds by reversed phase HPLC with diode array, finding the phenolic acids such as chlorogenic, gallic and caffeic, such as phenols responsible for the majority of araza antioxidant activity. It was noted that araza mesocarp polyphenol content was higher in the green (1200 mg/1000g fruto BH) state followed by mature (1200 mg/1000g fruto BH), pinton (905 mg/1000g fruto BH) and over mature (550 mg/1000g fruto BH) while the epicarp was greater in states mature (170 mg/1000g fruto BH) and green (295 mg/1000g fruto BH) followed of the over-mature (50 mg/1000g fruto BH) and pinton (100 mg/1000g fruto BH) states. The antioxidant capacity was determined by three hydrophilic methods (ABTS, DPPH and FRAP) in the mesocarp found that the antioxidant capacity was higher in the green state followed by mature), pinton and over mature while the epicarp was higher in mature, green, pinton) and over-mature state. We also determined the antioxidant capacity by a lipophilic method (b-carotene bleaching) found that in the mesocarp was higher in states followed by mature (110 mg/1000g fruto BH), pinton (90 mg/1000g fruto BH) and green (26 mg/1000g fruto BH) while in the epicarp was higher in the over mature stage (50 mg/1000g fruto BH)followed by greeen states (45 mg/1000g fruto BH), mature (55 mg/1000g fruto BH)and sobre mature (25 mg/1000g fruto BH). Finally we conclude that the mature and green had the highest antioxidant capacity, phenolic compounds being present in the epicarp and mesocarp were the major contributors. |
publishDate |
2013 |
dc.date.issued.spa.fl_str_mv |
2013 |
dc.date.accessioned.spa.fl_str_mv |
2019-06-29T09:20:03Z |
dc.date.available.spa.fl_str_mv |
2019-06-29T09:20:03Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/49804 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/43293/ |
url |
https://repositorio.unal.edu.co/handle/unal/49804 http://bdigital.unal.edu.co/43293/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/rcolquim/article/view/45885 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Química Revista Colombiana de Química |
dc.relation.ispartofseries.none.fl_str_mv |
Revista Colombiana de Química; Vol. 42, núm. 2 (2013): Versión preliminar; 213-235 2357-3791 0120-2804 |
dc.relation.references.spa.fl_str_mv |
Cuellar, Fabio A. and Ariza Cubillos, Edna Alejandra and Anzola, Cecilia and Restrepo, Patricia (2013) Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening. Revista Colombiana de Química; Vol. 42, núm. 2 (2013): Versión preliminar; 213-235 2357-3791 0120-2804 . |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia |
institution |
Universidad Nacional de Colombia |
bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/49804/1/45885-222568-1-SM.pdf https://repositorio.unal.edu.co/bitstream/unal/49804/2/45885-222568-1-SM.pdf.jpg |
bitstream.checksum.fl_str_mv |
715a33fb10043f37e96dff156d0822d4 9704d556b48b3047ae5a0eaab0aaf45e |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
repository.mail.fl_str_mv |
repositorio_nal@unal.edu.co |
_version_ |
1814089736966373376 |
spelling |
Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cuellar, Fabio A.b06a6f39-be91-4f01-950f-eb900153c677300Ariza Cubillos, Edna Alejandra6e18e2a1-6c13-4534-9414-d32657e2cc8f300Anzola, Cecilia4ed9e469-2044-44fc-9af0-3d91063a5a91300Restrepo, Patricia2c8b5188-b632-48e6-8f8a-0c24bcccbf953002019-06-29T09:20:03Z2019-06-29T09:20:03Z2013https://repositorio.unal.edu.co/handle/unal/49804http://bdigital.unal.edu.co/43293/The araza is a fruit native of the Amazon region, like other fruits of this region has some antioxidant components. This work was carried out the classification of the fruits of araza in four different states using as the index of maturity, then phenolic compounds were extracted using mixtures of solvents (methanol, acetone, water) and your content was determined by the spectrophotometric method Folin-Ciocalteu in the epicarp and mesocarp. Additionally, partially identified phenolic compounds by reversed phase HPLC with diode array, finding the phenolic acids such as chlorogenic, gallic and caffeic, such as phenols responsible for the majority of araza antioxidant activity. It was noted that araza mesocarp polyphenol content was higher in the green (1200 mg/1000g fruto BH) state followed by mature (1200 mg/1000g fruto BH), pinton (905 mg/1000g fruto BH) and over mature (550 mg/1000g fruto BH) while the epicarp was greater in states mature (170 mg/1000g fruto BH) and green (295 mg/1000g fruto BH) followed of the over-mature (50 mg/1000g fruto BH) and pinton (100 mg/1000g fruto BH) states. The antioxidant capacity was determined by three hydrophilic methods (ABTS, DPPH and FRAP) in the mesocarp found that the antioxidant capacity was higher in the green state followed by mature), pinton and over mature while the epicarp was higher in mature, green, pinton) and over-mature state. We also determined the antioxidant capacity by a lipophilic method (b-carotene bleaching) found that in the mesocarp was higher in states followed by mature (110 mg/1000g fruto BH), pinton (90 mg/1000g fruto BH) and green (26 mg/1000g fruto BH) while in the epicarp was higher in the over mature stage (50 mg/1000g fruto BH)followed by greeen states (45 mg/1000g fruto BH), mature (55 mg/1000g fruto BH)and sobre mature (25 mg/1000g fruto BH). Finally we conclude that the mature and green had the highest antioxidant capacity, phenolic compounds being present in the epicarp and mesocarp were the major contributors.El arazá es un fruto amazónico con buena capacidad antioxidante. En este trabajo se realizó, a partir del epicarpio y mesocarpio de la fruta en cuatro estados de madurez (verde, pintón, maduro y sobremaduro), la extracción de compuestos fenólicos (utilizando como solvente una mezcla de agua:acetona, ( inicial 50:50 y posterior de 70:30)) y su análisis (método colorimétrico de Folin-Ciocalteu), y se identificaron parcialmente por HPLC en fase reversa, con arreglo de diodos, encontrando los ácidos clorogénico, gálico y caféico como fenoles mayoritarios responsables de la actividad antioxidante. El contenido de polifenoles en el mesocarpio es mayor en el estado verde (1200mg/1000g fruto BH) seguido del maduro (1100mg/1000g fruto BH), pintón (905 mg/1000g fruto BH) y sobremaduro (550mg/1000g fruto BH), mientras que en el epicarpio es mayor en estados maduro (170 mg/1000g fruto BH) y verde (295mg/1000g fruto BH) seguidos del pintón (100 mg/1000g fruto BH) y sobremaduro (50 mg/1000g fruto BH). La capacidad antioxidante fue determinada por métodos hidrofílicos (ABTS, DPPH y FRAP) encontrando que en el mesocarpio la capacidad antioxidante medida por todos los métodos es mayor en estado verde seguido del maduro, pintón y sobremaduro mientras en el epicarpio es mayor en maduro y verde seguido del pintón y sobremaduro. Se determinó la capacidad antioxidante por un método lipofílico (decoloración del β-caroteno) encontrando que en el mesocarpio es mayor en estados maduro (110 mg/1000g fruto BH) y pintón (90 mg/1000g fruto BH) seguido del sobremaduro ( 75mg/1000g fruto BH) y verde (26mg/1000g fruto BH) y en el epicarpio es mayor en sobremaduro (50 mg/1000g fruto BH) seguido del verde (45 mg/1000g fruto BH), maduro (55 mg/1000g fruto BH) y pintón (25 mg/1000g fruto BH). Finalmente se concluye que el fruto de arazá en los estados verde y maduro presenta la mayor capacidad antioxidante, siendo los ácidos fenólicos los principales contribuyentes.application/pdfspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/rcolquim/article/view/45885Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de QuímicaRevista Colombiana de QuímicaRevista Colombiana de Química; Vol. 42, núm. 2 (2013): Versión preliminar; 213-235 2357-3791 0120-2804Cuellar, Fabio A. and Ariza Cubillos, Edna Alejandra and Anzola, Cecilia and Restrepo, Patricia (2013) Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripening. Revista Colombiana de Química; Vol. 42, núm. 2 (2013): Versión preliminar; 213-235 2357-3791 0120-2804 .Research of antioxidant capacity of araza (eugenia stipitata mc vaugh) during the ripeningArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTArazácapacidad antioxidanteABTSDPPHFRAPdecoloración del β-carotenomadurezArazaantioxidant capacityABTSDPPHFRAPβ-carotene bleachingripeningORIGINAL45885-222568-1-SM.pdfapplication/pdf871706https://repositorio.unal.edu.co/bitstream/unal/49804/1/45885-222568-1-SM.pdf715a33fb10043f37e96dff156d0822d4MD51THUMBNAIL45885-222568-1-SM.pdf.jpg45885-222568-1-SM.pdf.jpgGenerated Thumbnailimage/jpeg7604https://repositorio.unal.edu.co/bitstream/unal/49804/2/45885-222568-1-SM.pdf.jpg9704d556b48b3047ae5a0eaab0aaf45eMD52unal/49804oai:repositorio.unal.edu.co:unal/498042022-12-17 23:04:09.529Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |