Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche
ilustraciones, fotografías
- Autores:
-
Camacho Montoya, Luisa Fernanda
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/85828
- Palabra clave:
- 660 - Ingeniería química::664 - Tecnología de alimentos
570 - Biología::579 - Historia natural microorganismos, hongos, algas
Leche - Investigaciones
Bacteriología de la leche - Investigaciones
Análisis microbiológico
Técnicas inmunológicas
Enfermedades transmitidas por alimentos
Leche - Análisis - Normas
Conservación de alimentos
Enfermedades bacterianas
Salmonella Typhimurium
Staphylococcus aureus
Lactococcus lactis
Leche ultrapasteurizada
Leche cruda
Método horizontal
ELISA
Ultrapasteurized milk
Raw milk
Horizontal Method
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche |
| dc.title.translated.eng.fl_str_mv |
Evaluation of some traditional identification methods to detect Salmonella spp. and Staphylococcus aureus coagulase positive in milk |
| title |
Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche |
| spellingShingle |
Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche 660 - Ingeniería química::664 - Tecnología de alimentos 570 - Biología::579 - Historia natural microorganismos, hongos, algas Leche - Investigaciones Bacteriología de la leche - Investigaciones Análisis microbiológico Técnicas inmunológicas Enfermedades transmitidas por alimentos Leche - Análisis - Normas Conservación de alimentos Enfermedades bacterianas Salmonella Typhimurium Staphylococcus aureus Lactococcus lactis Leche ultrapasteurizada Leche cruda Método horizontal ELISA Ultrapasteurized milk Raw milk Horizontal Method |
| title_short |
Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche |
| title_full |
Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche |
| title_fullStr |
Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche |
| title_full_unstemmed |
Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche |
| title_sort |
Evaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en leche |
| dc.creator.fl_str_mv |
Camacho Montoya, Luisa Fernanda |
| dc.contributor.advisor.none.fl_str_mv |
Montoya Campuzano, Olga Inés Arango Isaza, Rafael Eduardo |
| dc.contributor.author.none.fl_str_mv |
Camacho Montoya, Luisa Fernanda |
| dc.contributor.researchgroup.spa.fl_str_mv |
Probióticos: Prospección Funcional y Metabolitos |
| dc.subject.ddc.spa.fl_str_mv |
660 - Ingeniería química::664 - Tecnología de alimentos 570 - Biología::579 - Historia natural microorganismos, hongos, algas |
| topic |
660 - Ingeniería química::664 - Tecnología de alimentos 570 - Biología::579 - Historia natural microorganismos, hongos, algas Leche - Investigaciones Bacteriología de la leche - Investigaciones Análisis microbiológico Técnicas inmunológicas Enfermedades transmitidas por alimentos Leche - Análisis - Normas Conservación de alimentos Enfermedades bacterianas Salmonella Typhimurium Staphylococcus aureus Lactococcus lactis Leche ultrapasteurizada Leche cruda Método horizontal ELISA Ultrapasteurized milk Raw milk Horizontal Method |
| dc.subject.lemb.none.fl_str_mv |
Leche - Investigaciones Bacteriología de la leche - Investigaciones Análisis microbiológico Técnicas inmunológicas Enfermedades transmitidas por alimentos Leche - Análisis - Normas Conservación de alimentos Enfermedades bacterianas |
| dc.subject.proposal.spa.fl_str_mv |
Salmonella Typhimurium Staphylococcus aureus Lactococcus lactis Leche ultrapasteurizada Leche cruda Método horizontal ELISA |
| dc.subject.proposal.eng.fl_str_mv |
Ultrapasteurized milk Raw milk Horizontal Method |
| description |
ilustraciones, fotografías |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-03-22T18:40:12Z |
| dc.date.available.none.fl_str_mv |
2024-03-22T18:40:12Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/85828 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/85828 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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LaReferencia |
| dc.relation.references.spa.fl_str_mv |
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Food Microbiology, 91, 103541. https://doi.org/10.1016/J.FM.2020.103541 Argudín, M. A., Mendoza, M. C., & Rodicio, M. R. (2010). Food Poisoning and Staphylococcus aureus Enterotoxins. Toxins, 2(7), 1751–1773. https://doi.org/10.3390/toxins2071751 Batista Gutiérrez, L., Manuel ROSADO GARCÍA, F., Isabel GONZÁLEZ GONZÁLEZ, M., & Villar Saavedra, A. (2019). IMPLEMENTATION OF A SCREENING TECHNIQUE FOR THE IDENTIFICATION OF SALMONELLA SPP. IN SURFACE WATER. Higiene y Sanidad Ambiental, 19(2), 1749–1754 Bianchi, D. M., Maurella, C., Lenzi, C., Fornasiero, M., Barbaro, A., & Decastelli, L. (2022). Influence of Season and Food Type on Bacterial and Entero-Toxigenic Prevalence of Staphylococcus aureus. Toxins 2022, Vol. 14, Page 671, 14(10), 671. https://doi.org/10.3390/TOXINS14100671 Bordin, G., Cordeiro Raposo, F., De La Calle, B., & Rodriguez, A. R. (2001). Identification and quantification of major bovine milk proteins by liquid chromatography. Journal of Chromatography A, 928(1), 63–76. https://doi.org/10.1016/S0021-9673(01)01097-4 Camaró-Sala, M. L., Martínez-García, R., Olmos-Martínez, P., Catalá-Cuenca, V., Ocete-Mochón, M. D., & Gimeno-Cardona, C. (2015). Validation and verfication of microbiology methods | Validación y verificación analítica de los métodos microbiológicos. Enfermedades Infecciosas y Microbiologia Clinica Monografias, 33(7), e31–e36. https://doi.org/10.1016/j.eimc.2013.11.010 Castro, S. T., Rodríguez, C. R., Perazzi, B. E., Radice, M., Paz Sticotti, M., Muzio, H., Juárez, J., Gutkind, G., Famiglietti, A. M. R., Santini, P. I., & Vay, C. A. (2006). Comparación de diferentes métodos para identificar las especies del género Proteus. Revista Argentina de Microbiología v.38 n.3 Ciudad Autónoma de Buenos Aires. http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412006000300002 Centers for Disease Control and Prevention. (2021, July 16). Microbios y enfermedades transmitidos por los alimentos | Seguridad alimenticia | CDC. https://www.cdc.gov/foodsafety/es/foodborne-germs-es.html Centers for Disease Control and Prevention (CDC). (2019). Salmonella | CDC. Salmonella . https://www.cdc.gov/salmonella/index.html Chacón, L., Barrantes, K., García, C., & Rosario, A. (2010). Estandarización de una PCR para la detección del gen invA de Salmonella spp. en lechuga. Revista de La Sociedad Venezolana de Microbiología, 30(1), 18–23. http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S1315-25562010000100005&lng=es&nrm=iso&tlng=es Chambers, H. F., & DeLeo, F. R. (2009). Waves of resistance: Staphylococcus aureus in the antibiotic era. Nature Reviews Microbiology, 7(9), 629–641. https://doi.org/10.1038/nrmicro2200 Codex Alimentarius. (2011). Leche y Productos Lácteos Segunda edición. http://www.codexalimentarius.org Corral, A., Morales, Y., Pazos, L., Ramírez, A., Martínez, R., & Muñoz, J. (2012). Quantification of cultivable bacteria by the “Massive Stamping Drop Plate” method. Revista Colombiana Biotecnología, 2, 147–156 Côté-Gravel, J., & Malouin, F. (2019). Symposium review: Features of Staphylococcus aureus mastitis pathogenesis that guide vaccine development strategies. Journal of Dairy Science, 102(5), 4727–4740. https://doi.org/10.3168/JDS.2018-15272 David, A., Porras Atencia, O. O., Bermúdez, S. C., Velasco Sánchez, N. J., & Osorio Padilla, M. L. (2016). Detección de Listeria ssp y Salmonella spp en queso y su relación con las caraterísticas fisicoquímicas. Revista Politécnica, Vol. 12, No. 23. https://revistas.elpoli.edu.co/index.php/pol/article/view/903 Dhanashekar, R., Akkinepalli, S., & Nellutla, A. (2012). Milk-borne infections. An analysis of their potential effect on the milk industry. Germs, 2(3), 101. https://doi.org/10.11599/GERMS.2012.1020 Dos Santos, P. H. C., Figueiredo, H. M., da SILVA, L. H. M., da SILVA, R. S. O., Cardoso, G. V. F., Moraes, C. M., & Rodrigues, A. M. da C. (2020). Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon. Food Science and Technology, 41(1), 151–157. https://doi.org/10.1590/FST.38719 Doyle, M. P., Diez-Gonzalez, F., & Hill, C. (2019). Food microbiology: Fundamentals and frontiers. In Food Microbiology: Fundamentals and Frontiers. https://doi.org/10.1002/9781683670476 Drake, P., Chen, Y.-C., Lehmann, I., & Jiang, P.-S. (2015). Nanoparticle labels for pathogen detection through nucleic acid amplification tests. Microfluidics and Nanofluidics, 19(2), 299–305. https://doi.org/10.1007/s10404-014-1530-8 Ertas, N., Gonulalan, Z., Yildirim, Y., & Kum, E. (2010). Detection of Staphylococcus aureus enterotoxins in sheep cheese and dairy desserts by multiplex PCR technique. International Journal of Food Microbiology, 142(1–2), 74–77. https://doi.org/10.1016/J.IJFOODMICRO.2010.06.002 European Commission. (2005). Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. https://eur-lex.europa.eu/legal-content/ES/TXT/PDF/?uri=CELEX:02005R2073-20180101&from=EN Fàbrega, A., & Vila, J. (2013). Salmonella enterica serovar Typhimurium skills to succeed in the host: Virulence and regulation. Clinical Microbiology Reviews, 26(2), 308–341. https://doi.org/10.1128/CMR.00066-12 Foster, T. J. (2009). Colonization and infection of the human host by staphylococci: Adhesion, survival and immune evasion. Veterinary Dermatology, 20(5–6), 456–470. https://doi.org/10.1111/j.1365-3164.2009.00825.x Galván, M. del pilar. (2005). PROCESO BÁSICO DE LA LECHE Y EL QUESO. Revista Digital Universitaria, 6. http://www.revista.unam.mx/vol.6/num9/art87/int87.htm GE Healthcare. (2007). Illustra bacteria genomicPrep Mini Spin Kit Gökmen-Polar, Y. (2018). Overview of PCR-Based Technologies and Multiplexed Gene Analysis for Biomarker Studies. 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G. D. A. (2005). Modern Food Microbiology Jayarao, B. M., & Henning, D. R. (2001). Prevalence of foodborne pathogens in bulk tank milk. Journal of Dairy Science, 84(10), 2157–2162. https://doi.org/10.3168/jds.S0022-0302(01)74661-9 Kav, K., Col, R., & Ardic, M. (2011). Characterization of Staphylococcus aureus Isolates from White-Brined Urfa Cheese. Journal of Food Protection, 74(11), 1788–1796. https://doi.org/10.4315/0362-028X.JFP-11-179 Kirk, M. D., Pires, S. M., Black, R. E., Caipo, M., Crump, J. A., Devleesschauwer, B., Döpfer, D., Fazil, A., Fischer-Walker, C. L., Hald, T., Havelaar, A. H., & Angulo, F. J. (2015). World Health Organization Estimates of the Global and Regional Disease Burden of 22 Foodborne Bacterial, Protozoal, and Viral Diseases, 2010: A Data Synthesis. PLoS Medicine, 12(12). https://doi.org/10.1371/journal.pmed.1001921 Kong, J., Fan, C., Liao, X., Chen, A., Yang, S., Zhao, L., & Li, H. (2022). Accurate detection of Escherichia coli O157:H7 and Salmonella enterica serovar typhimurium based on the combination of next-generation sequencing and droplet digital PCR. LWT, 168, 113913. https://doi.org/10.1016/J.LWT.2022.113913 Koskinen, M. T., Wellenberg, G. J., Sampimon, O. C., Holopainen, J., Rothkamp, A., Salmikivi, L., van Haeringen, W. A., Lam, T. J. G. M., & Pyörälä, S. (2010). Field comparison of real-time polymerase chain reaction and bacterial culture for identification of bovine mastitis bacteria. Journal of Dairy Science, 93(12), 5707–5715. https://doi.org/10.3168/JDS.2010-3167 Laboratorios Britania. (2012). T.S.I. Agar (Triple Sugar Iron Agar). https://www.britanialab.com/back/public/upload/productos/upl_6070971eb11bd.pdf Laboratorios Britania. (2021a). Baird Parker Agar Base. https://www.britanialab.com/back/public/upload/productos/upl_607060f71db9b.pdf Laboratorios Britania. (2021b). Christensen Medio (Urea Agar Base). https://www.britanialab.com/back/public/upload/productos/upl_6054e95043249.pdf Laboratorios Britania. (2021c). Lisina Hierro Agar. https://www.britanialab.com/back/public/upload/productos/upl_6054e85d48c47.pdf Laboratorios Britania. (2021d). Simmons Citrato Agar. https://www.britanialab.com/back/public/upload/productos/upl_607092758cfa8.pdf Luigi, T., Rojas, L., & Valbuena, O. (2015). Polymerase chain reaction (PCR) for identification of Salmonella spp. using the invA gene | Reacción en cadena de la polimerasa para la identificación de Salmonella spp. Usando el gen invA. Salus, 19(3), 41–46 MacFaddin, J. F. (2003). Pruebas bioquímicas para la identificación de bacterias de importancia clínica Makovec, J. A., & Ruegg, P. L. (2003). Results of Milk Samples Submitted for Microbiological Examination in Wisconsin from 1994 to 2001. Journal of Dairy Science, 86(11), 3466–3472. https://doi.org/10.3168/JDS.S0022-0302(03)73951-4 Microbiologics. (2023). 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The effect of pre-enrichment protocol on the sensitivity and specificity of PCR for detection of naturally contaminated Salmonella in raw poultry compared to conventional culture. Food Microbiology, 23(6), 599–604. https://doi.org/10.1016/J.FM.2005.09.002 Neelam, Jain, V. K., Singh, M., Joshi, V. G., Chhabra, R., Singh, K., & Rana, Y. S. (2022). Virulence and antimicrobial resistance gene profiles of Staphylococcus aureus associated with clinical mastitis in cattle. PLOS ONE, 17(5), e0264762. https://doi.org/10.1371/JOURNAL.PONE.0264762 Nia, Y., Lombard, B., Gentil, S., Neveux, L., Mutel, I., Guillier, F., Messio, S., Pairaud, S., Herbin, S., Guillier, L., Auvray, F., & Hennekinne, J. A. (2021). Development and validation of the Standard method EN ISO 19020 - microbiology of the food chain — Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs. 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Intra‐laboratory validation of the Ridascreen® SET Total kit for detecting staphylococcal enterotoxins SEA to SEE in cheese. Letters in Applied Microbiology, 52(5), 468–474. https://doi.org/10.1111/J.1472-765X.2011.03025.X Paco González Ulibarry. (2018). Definiciones de Leche y Queso. Biblioteca Del Congreso Nacional de Chile/BCN-Asesoría Técnica Parlamentaria. http://bcn.cl/22oic Paniel, N., & Noguer, T. (2019). Detection of Salmonella in Food Matrices, from Conventional Methods to Recent Aptamer-Sensing Technologies. Foods 2019, Vol. 8, Page 371, 8(9), 371. https://doi.org/10.3390/FOODS8090371 Park, C. E., Warburton, D., & Laffey, P. J. (1996). A collaborative study on the detection of staphylococcal enterotoxins in foods by an enzyme immunoassay kit (RIDASCREEN®). International Journal of Food Microbiology, 29(2–3), 281–295. https://doi.org/10.1016/0168-1605(95)00046-1 Parra, M., Durango, J., Máttar, S., & De Tema, R. (2002). 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Identificación de bacterias acidolácticas antagónicas de Salmonella enterica var. Typhimurium aisladas de queso artesanal. Revista Mexicana de Ciencias Agrícolas, 8(4), 785–797. https://doi.org/10.29312/remexca.v8i4.7 Rosec, J. P., & Gigaud, O. (2002). Staphylococcal enterotoxin genes of classical and new types detected by PCR in France. International Journal of Food Microbiology, 77(1–2), 61–70. https://doi.org/10.1016/S0168-1605(02)00044-2 Sartori, C., Boss, R., Ivanovic, I., & Graber, H. U. (2017). Development of a new real-time quantitative PCR assay for the detection of Staphylococcus aureus genotype B in cow milk, targeting the new gene adlb. Journal of Dairy Science, 100(10), 7834–7845. https://doi.org/10.3168/JDS.2017-12820 Scallan, E., Hoekstra, R. M., Angulo, F. J., Tauxe, R. V., Widdowson, M.-A., Roy, S. L., Jones, J. L., & Griffin, P. M. (2011a). Foodborne illness acquired in the United States-Major pathogens. 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Atribución-NoComercial-SinDerivadas 4.0 Internacional |
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77 páginas |
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Universidad Nacional de Colombia |
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Medellín - Ciencias - Maestría en Ciencias - Biotecnología |
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Facultad de Ciencias |
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Medellín, Colombia |
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Universidad Nacional de Colombia - Sede Medellín |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Montoya Campuzano, Olga Inés4b04a4a60b5a0df6e629979838d9c079Arango Isaza, Rafael Eduardob7e61322453072ebf7408a0f587e39c1Camacho Montoya, Luisa Fernandab64e16afb91cef59d54438fd34f9815fProbióticos: Prospección Funcional y Metabolitos2024-03-22T18:40:12Z2024-03-22T18:40:12Z2023https://repositorio.unal.edu.co/handle/unal/85828Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografíasLa leche es una matriz alimenticia con gran contenido nutricional, lo que estimula el crecimiento de bacterias como Salmonella Typhimurium y Staphylococcus aureus de alto impacto en la seguridad alimentaria, además, con una alta prevalencia en la leche, por lo que es necesario establecer metodologías para su detección. En este estudio se inocularon muestras de leche con estos microorganismos para establecer la capacidad del método horizontal, inmunológico (ELISA) y molecular (PCR) para detectarlos, para ello, se evaluó la detección del método horizontal inoculando simultáneamente S. Typhimurium.y S. aureus en leche cruda y ultrapasteurizada (UHT), también se realizaron pruebas inoculando leche UHT con estos dos microorganismos y la Bacteria ácido láctica (BAL) de la especie Lactococcus lactis para evaluar la detección de ellos en presencia de una bacteria que puede hacer parte de la microbiota de la leche. Con los métodos horizontal y ELISA se identificó S. Typhimurium en todas las muestras, mientras que la PCR no logró su identificación. Por otro lado, S. aureus solo se identificó por el método horizontal con un 90% de detección. En la inoculación simultánea se compararon los resultados con el recuento de UFC/mL de S. aureus, debido a que para S. Typhimurium no se requiere recuento sino ausencia/presencia. Se encontró que en la muestra de leche UHT inoculada con S. Typhimurium.y S. aureus se observó un mayor recuento que en la leche cruda, lo que indica que la microbiota presente en la leche cruda pudo ejercer un efecto inhibidor sobre el crecimiento de S. aureus; en contraste, la leche UHT inoculada adicionalmente con L. lactis, tuvo un mayor crecimiento que en todas las inoculaciones, lo que sugiere algún tipo de interacción entre los tres microorganismos. (Tomado de la fuente)Milk is a nutritional matrix with high nutritional content, which promotes the growth of bacteria such as Salmonella Typhimurium and Staphylococcus aureus, having a significant impact on food safety. Moreover, these bacteria have a high prevalence in milk, making it necessary to establish methodologies for their detection. In this study, milk samples were inoculated with these microorganisms to assess the capability of the horizontal, immunological (ELISA), and molecular (PCR) methods for detection. The horizontal method detection was evaluated by simultaneously inoculating S. Typhimurium and S. aureus in raw and ultra-pasteurized milk (UHT). Tests were also conducted by inoculating UHT milk with these two microorganisms and lactic acid bacteria (LAB) of the Lactococcus lactis species to evaluate their detection in the presence of bacteria that may be part of milk's microbiota. Using the horizontal and ELISA methods, S. Typhimurium was identified in all samples, while PCR failed to identify it. On the other hand, S. aureus was only identified by the horizontal method with a detection rate of 90%. In the simultaneous inoculation, the results were compared with the S. aureus colony-forming units per milliliter (CFU/mL) count, as S. Typhimurium requires absence/presence rather than a count. It was found that in the UHT milk sample inoculated with S. Typhimurium and S. aureus, a higher count was observed than in raw milk, indicating that the microbiota present in raw milk could exert an inhibitory effect on the growth of S. aureus. In contrast, UHT milk inoculated additionally with L. lactis had higher growth than in all inoculations, suggesting some type of interaction among the three microorganisms.MaestríaMagíster en Ciencias - BiotecnologíaMicrobiología de alimentosBiotecnología.Sede Medellín77 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín660 - Ingeniería química::664 - Tecnología de alimentos570 - Biología::579 - Historia natural microorganismos, hongos, algasLeche - InvestigacionesBacteriología de la leche - InvestigacionesAnálisis microbiológicoTécnicas inmunológicasEnfermedades transmitidas por alimentosLeche - Análisis - NormasConservación de alimentosEnfermedades bacterianasSalmonella TyphimuriumStaphylococcus aureusLactococcus lactisLeche ultrapasteurizadaLeche crudaMétodo horizontalELISAUltrapasteurized milkRaw milkHorizontal MethodEvaluación de algunos métodos tradicionales de identificación para detectar Salmonella spp. y Staphylococcus aureus coagulasa positiva en lecheEvaluation of some traditional identification methods to detect Salmonella spp. and Staphylococcus aureus coagulase positive in milkTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMLaReferenciaAgarwal, A., Awasthi, V., Dua, A., Ganguly, S., Garg, V., & Marwaha, S. 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Analytica Chimica Acta, 638(2), 115–125. https://doi.org/10.1016/j.aca.2009.02.038EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85828/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1035428317.2023.pdf1035428317.2023.pdfTesis de Maestría en Biotecnologíaapplication/pdf1313937https://repositorio.unal.edu.co/bitstream/unal/85828/2/1035428317.2023.pdf7994a618671a5e0e0d2e4e1713b9459dMD52THUMBNAIL1035428317.2023.pdf.jpg1035428317.2023.pdf.jpgGenerated Thumbnailimage/jpeg5385https://repositorio.unal.edu.co/bitstream/unal/85828/3/1035428317.2023.pdf.jpg57f88aaf5df1436b98064bdb94f71b96MD53unal/85828oai:repositorio.unal.edu.co:unal/858282024-08-23 23:11:32.34Repositorio Institucional Universidad Nacional de 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