Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
Se presenta la secuenciación y caracterización del genoma de un aislado natural de levadura Saccharomyces cerevisiae, identificada como una cepa con potencial para la producción de etanol de segunda generación. La caracterización molecular se llevó a cabo mediante la secuenciación del genoma con la...
- Autores:
-
Cifuentes Triana, Yina Alejandra
- Tipo de recurso:
- Fecha de publicación:
- 2016
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/58629
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/58629
http://bdigital.unal.edu.co/55418/
- Palabra clave:
- 57 Ciencias de la vida; Biología / Life sciences; biology
Etanol celulósico
S. cerevisiae
Secuenciación genómica
Consumo xilosa
Cellulosic ethanol
Genome sequencing
Xylose consumption
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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|
dc.title.spa.fl_str_mv |
Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación |
title |
Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación |
spellingShingle |
Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación 57 Ciencias de la vida; Biología / Life sciences; biology Etanol celulósico S. cerevisiae Secuenciación genómica Consumo xilosa Cellulosic ethanol Genome sequencing Xylose consumption |
title_short |
Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación |
title_full |
Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación |
title_fullStr |
Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación |
title_full_unstemmed |
Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación |
title_sort |
Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación |
dc.creator.fl_str_mv |
Cifuentes Triana, Yina Alejandra |
dc.contributor.advisor.spa.fl_str_mv |
Velásquez Lozano, Mario Enrique (Thesis advisor) Pinzón Velásco, Andrés Mauricio (Thesis advisor) |
dc.contributor.author.spa.fl_str_mv |
Cifuentes Triana, Yina Alejandra |
dc.subject.ddc.spa.fl_str_mv |
57 Ciencias de la vida; Biología / Life sciences; biology |
topic |
57 Ciencias de la vida; Biología / Life sciences; biology Etanol celulósico S. cerevisiae Secuenciación genómica Consumo xilosa Cellulosic ethanol Genome sequencing Xylose consumption |
dc.subject.proposal.spa.fl_str_mv |
Etanol celulósico S. cerevisiae Secuenciación genómica Consumo xilosa Cellulosic ethanol Genome sequencing Xylose consumption |
description |
Se presenta la secuenciación y caracterización del genoma de un aislado natural de levadura Saccharomyces cerevisiae, identificada como una cepa con potencial para la producción de etanol de segunda generación. La caracterización molecular se llevó a cabo mediante la secuenciación del genoma con la plataforma Hiseq Illumina 2000 finales pareados. |
publishDate |
2016 |
dc.date.issued.spa.fl_str_mv |
2016-12-19 |
dc.date.accessioned.spa.fl_str_mv |
2019-07-02T14:30:43Z |
dc.date.available.spa.fl_str_mv |
2019-07-02T14:30:43Z |
dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
status_str |
acceptedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/58629 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/55418/ |
url |
https://repositorio.unal.edu.co/handle/unal/58629 http://bdigital.unal.edu.co/55418/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Sede Bogotá Facultad de Ciencias Posgrado Interfacultades en Microbiología Posgrado Interfacultades en Microbiología |
dc.relation.references.spa.fl_str_mv |
Cifuentes Triana, Yina Alejandra (2016) Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación. Maestría thesis, Universidad Nacional de Colombia - Sede Bogotá. |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Velásquez Lozano, Mario Enrique (Thesis advisor)aabcfa5c-22d1-4ad7-b7e9-a8d89673839e-1Pinzón Velásco, Andrés Mauricio (Thesis advisor)d838a83c-f796-4719-ad01-c5961753640c-1Cifuentes Triana, Yina Alejandra4a7b1cb9-2467-4d95-9e1a-93e6df3fcfbf3002019-07-02T14:30:43Z2019-07-02T14:30:43Z2016-12-19https://repositorio.unal.edu.co/handle/unal/58629http://bdigital.unal.edu.co/55418/Se presenta la secuenciación y caracterización del genoma de un aislado natural de levadura Saccharomyces cerevisiae, identificada como una cepa con potencial para la producción de etanol de segunda generación. La caracterización molecular se llevó a cabo mediante la secuenciación del genoma con la plataforma Hiseq Illumina 2000 finales pareados.Abstract. In this work the sequencing and genome characterization of a natural isolate of Saccharomyces cerevisiae yeast (strain 202-3), identified with potential for the production of second generation ethanol is presented. In previous studies conducted by the Research Group of Chemical and Biochemical Processes focused on the production of second-generation ethanol from sugarcane bagasse hydrolysates, we identified a group of isolates of S. cerevisiae with a consumption of xylose (sugar present in hydrolysates) of 2-5% for strain 202-3, this being an advantage for the fermentation of hydrolysates, since many strains of S. cerevisiae are incapable of processing. This advantage and other prominent positive aspects during fermentation profiles evaluated bagasse hydrolysates made the strain 202-3 a candidate strain to improve the production of second-generation ethanol, which was proposed as a first step to study the strain at the genomic level. The molecular characterization was carried out by genome sequencing with the Illumina HiSeq 2000 platform paired end; the assembly was performed with different programs, finally choosing the assembler ABYSS with kmer 89. Gene prediction was developed with the approach of hidden Markov models with Augustus. The genes identified were scored based on similarity with public databases of nucleotide and protein. Records were organized from ontological functions at different hierarchical levels, which identified central metabolic functions and roles of the S. cerevisiae strain 202-3, highlighting the presence of four possible new proteins, two of them probably associated with the positive consumption of xylose.Maestríaapplication/pdfspaUniversidad Nacional de Colombia Sede Bogotá Facultad de Ciencias Posgrado Interfacultades en MicrobiologíaPosgrado Interfacultades en MicrobiologíaCifuentes Triana, Yina Alejandra (2016) Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación. Maestría thesis, Universidad Nacional de Colombia - Sede Bogotá.57 Ciencias de la vida; Biología / Life sciences; biologyEtanol celulósicoS. cerevisiaeSecuenciación genómicaConsumo xilosaCellulosic ethanolGenome sequencingXylose consumptionSecuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generaciónTrabajo de grado - 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