Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación

Se presenta la secuenciación y caracterización del genoma de un aislado natural de levadura Saccharomyces cerevisiae, identificada como una cepa con potencial para la producción de etanol de segunda generación. La caracterización molecular se llevó a cabo mediante la secuenciación del genoma con la...

Full description

Autores:
Cifuentes Triana, Yina Alejandra
Tipo de recurso:
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/58629
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/58629
http://bdigital.unal.edu.co/55418/
Palabra clave:
57 Ciencias de la vida; Biología / Life sciences; biology
Etanol celulósico
S. cerevisiae
Secuenciación genómica
Consumo xilosa
Cellulosic ethanol
Genome sequencing
Xylose consumption
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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oai_identifier_str oai:repositorio.unal.edu.co:unal/58629
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network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
title Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
spellingShingle Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
57 Ciencias de la vida; Biología / Life sciences; biology
Etanol celulósico
S. cerevisiae
Secuenciación genómica
Consumo xilosa
Cellulosic ethanol
Genome sequencing
Xylose consumption
title_short Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
title_full Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
title_fullStr Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
title_full_unstemmed Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
title_sort Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación
dc.creator.fl_str_mv Cifuentes Triana, Yina Alejandra
dc.contributor.advisor.spa.fl_str_mv Velásquez Lozano, Mario Enrique (Thesis advisor)
Pinzón Velásco, Andrés Mauricio (Thesis advisor)
dc.contributor.author.spa.fl_str_mv Cifuentes Triana, Yina Alejandra
dc.subject.ddc.spa.fl_str_mv 57 Ciencias de la vida; Biología / Life sciences; biology
topic 57 Ciencias de la vida; Biología / Life sciences; biology
Etanol celulósico
S. cerevisiae
Secuenciación genómica
Consumo xilosa
Cellulosic ethanol
Genome sequencing
Xylose consumption
dc.subject.proposal.spa.fl_str_mv Etanol celulósico
S. cerevisiae
Secuenciación genómica
Consumo xilosa
Cellulosic ethanol
Genome sequencing
Xylose consumption
description Se presenta la secuenciación y caracterización del genoma de un aislado natural de levadura Saccharomyces cerevisiae, identificada como una cepa con potencial para la producción de etanol de segunda generación. La caracterización molecular se llevó a cabo mediante la secuenciación del genoma con la plataforma Hiseq Illumina 2000 finales pareados.
publishDate 2016
dc.date.issued.spa.fl_str_mv 2016-12-19
dc.date.accessioned.spa.fl_str_mv 2019-07-02T14:30:43Z
dc.date.available.spa.fl_str_mv 2019-07-02T14:30:43Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/58629
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/55418/
url https://repositorio.unal.edu.co/handle/unal/58629
http://bdigital.unal.edu.co/55418/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Sede Bogotá Facultad de Ciencias Posgrado Interfacultades en Microbiología
Posgrado Interfacultades en Microbiología
dc.relation.references.spa.fl_str_mv Cifuentes Triana, Yina Alejandra (2016) Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación. Maestría thesis, Universidad Nacional de Colombia - Sede Bogotá.
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
institution Universidad Nacional de Colombia
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repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv repositorio_nal@unal.edu.co
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Velásquez Lozano, Mario Enrique (Thesis advisor)aabcfa5c-22d1-4ad7-b7e9-a8d89673839e-1Pinzón Velásco, Andrés Mauricio (Thesis advisor)d838a83c-f796-4719-ad01-c5961753640c-1Cifuentes Triana, Yina Alejandra4a7b1cb9-2467-4d95-9e1a-93e6df3fcfbf3002019-07-02T14:30:43Z2019-07-02T14:30:43Z2016-12-19https://repositorio.unal.edu.co/handle/unal/58629http://bdigital.unal.edu.co/55418/Se presenta la secuenciación y caracterización del genoma de un aislado natural de levadura Saccharomyces cerevisiae, identificada como una cepa con potencial para la producción de etanol de segunda generación. La caracterización molecular se llevó a cabo mediante la secuenciación del genoma con la plataforma Hiseq Illumina 2000 finales pareados.Abstract. In this work the sequencing and genome characterization of a natural isolate of Saccharomyces cerevisiae yeast (strain 202-3), identified with potential for the production of second generation ethanol is presented. In previous studies conducted by the Research Group of Chemical and Biochemical Processes focused on the production of second-generation ethanol from sugarcane bagasse hydrolysates, we identified a group of isolates of S. cerevisiae with a consumption of xylose (sugar present in hydrolysates) of 2-5% for strain 202-3, this being an advantage for the fermentation of hydrolysates, since many strains of S. cerevisiae are incapable of processing. This advantage and other prominent positive aspects during fermentation profiles evaluated bagasse hydrolysates made the strain 202-3 a candidate strain to improve the production of second-generation ethanol, which was proposed as a first step to study the strain at the genomic level. The molecular characterization was carried out by genome sequencing with the Illumina HiSeq 2000 platform paired end; the assembly was performed with different programs, finally choosing the assembler ABYSS with kmer 89. Gene prediction was developed with the approach of hidden Markov models with Augustus. The genes identified were scored based on similarity with public databases of nucleotide and protein. Records were organized from ontological functions at different hierarchical levels, which identified central metabolic functions and roles of the S. cerevisiae strain 202-3, highlighting the presence of four possible new proteins, two of them probably associated with the positive consumption of xylose.Maestríaapplication/pdfspaUniversidad Nacional de Colombia Sede Bogotá Facultad de Ciencias Posgrado Interfacultades en MicrobiologíaPosgrado Interfacultades en MicrobiologíaCifuentes Triana, Yina Alejandra (2016) Secuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generación. Maestría thesis, Universidad Nacional de Colombia - Sede Bogotá.57 Ciencias de la vida; Biología / Life sciences; biologyEtanol celulósicoS. cerevisiaeSecuenciación genómicaConsumo xilosaCellulosic ethanolGenome sequencingXylose consumptionSecuenciación y caracterización del genoma de la cepa Saccharomyces cerevisiae 202-3 con potencial para la producción de etanol de segunda generaciónTrabajo de grado - 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