A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
Cocoa, Theobroma cacao L. is one of the main tropical industrial crops. Cocoa has a very high level of interfering substances, such as polysaccharides and phenolic compounds that could prevent the isolation of suitable protein. Efficient methods of protein extraction are a priority to successfully a...
- Autores:
-
Martínez-Márquez, Ascensión
Morante-Carriel, Jaime Alfredo
Bru-Martínez, Roque
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/61091
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/61091
http://bdigital.unal.edu.co/59899/
- Palabra clave:
- 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Cocoa pod
One-dimensional electrophoresis
Phenol/SDS extraction protocols
Proteome analyses
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
id |
UNACIONAL2_c52b7df8a109d581ff6764c41201948b |
---|---|
oai_identifier_str |
oai:repositorio.unal.edu.co:unal/61091 |
network_acronym_str |
UNACIONAL2 |
network_name_str |
Universidad Nacional de Colombia |
repository_id_str |
|
spelling |
Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Martínez-Márquez, Ascensión072ac28e-22c8-438a-bfe1-9715d825d378300Morante-Carriel, Jaime Alfredof78d932e-b00a-4ad3-92e4-1c90eb1bcc60300Bru-Martínez, Roque620a9f4a-87c8-476a-8a9e-1663f2629c973002019-07-02T19:54:08Z2019-07-02T19:54:08Z2017-04-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/61091http://bdigital.unal.edu.co/59899/Cocoa, Theobroma cacao L. is one of the main tropical industrial crops. Cocoa has a very high level of interfering substances, such as polysaccharides and phenolic compounds that could prevent the isolation of suitable protein. Efficient methods of protein extraction are a priority to successfully apply proteomic analyses. We compared and evaluated two methods (A and B) of tissue preparation for total protein extract by phenol/SDS extraction protocol. The difference in the application of the two methods was that extensively washed dry powder of pod tissue were made in Method A, whereas that crude extract were prepared Method B. Extracted proteins were examined using one-dimensional electrophoresis (1-D). Results show that each extraction method isolated a unique subset of cocoa pod proteome. Principal component analysis showed little variation in the data obtained using Method A, while that in Methods B showed no low reproducibility, thus demonstrating that Method A is a reliable for preparing cocoa pod proteins. The protocol is expected to be applicable to other recalcitrant plant tissues and to be of interest to laboratories involved in plant proteomics analyses. A combination of extraction approaches is recommended for increasing proteome coverage when using gel-based isolation techniques.application/pdfspaUniversidad Nacional de Colombia - Sede Palmirahttps://revistas.unal.edu.co/index.php/acta_agronomica/article/view/54598Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaMartínez-Márquez, Ascensión and Morante-Carriel, Jaime Alfredo and Bru-Martínez, Roque (2017) A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod. Acta Agronómica, 66 (2). 248 - 253. ISSN 2323-011855 Ciencias de la tierra / Earth sciences and geology63 Agricultura y tecnologías relacionadas / AgricultureCocoa podOne-dimensional electrophoresisPhenol/SDS extraction protocolsProteome analysesA comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) podArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL54598-311480-5-PB.pdfapplication/pdf13107306https://repositorio.unal.edu.co/bitstream/unal/61091/1/54598-311480-5-PB.pdf6e09a93c43cd2a7ead3ca1da4a3ab5caMD51THUMBNAIL54598-311480-5-PB.pdf.jpg54598-311480-5-PB.pdf.jpgGenerated Thumbnailimage/jpeg7585https://repositorio.unal.edu.co/bitstream/unal/61091/2/54598-311480-5-PB.pdf.jpgd1b8f92f0b382743c769af6be7a3b93cMD52unal/61091oai:repositorio.unal.edu.co:unal/610912024-04-16 23:38:41.476Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |
dc.title.spa.fl_str_mv |
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod |
title |
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod |
spellingShingle |
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod 55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture Cocoa pod One-dimensional electrophoresis Phenol/SDS extraction protocols Proteome analyses |
title_short |
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod |
title_full |
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod |
title_fullStr |
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod |
title_full_unstemmed |
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod |
title_sort |
A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod |
dc.creator.fl_str_mv |
Martínez-Márquez, Ascensión Morante-Carriel, Jaime Alfredo Bru-Martínez, Roque |
dc.contributor.author.spa.fl_str_mv |
Martínez-Márquez, Ascensión Morante-Carriel, Jaime Alfredo Bru-Martínez, Roque |
dc.subject.ddc.spa.fl_str_mv |
55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture |
topic |
55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture Cocoa pod One-dimensional electrophoresis Phenol/SDS extraction protocols Proteome analyses |
dc.subject.proposal.spa.fl_str_mv |
Cocoa pod One-dimensional electrophoresis Phenol/SDS extraction protocols Proteome analyses |
description |
Cocoa, Theobroma cacao L. is one of the main tropical industrial crops. Cocoa has a very high level of interfering substances, such as polysaccharides and phenolic compounds that could prevent the isolation of suitable protein. Efficient methods of protein extraction are a priority to successfully apply proteomic analyses. We compared and evaluated two methods (A and B) of tissue preparation for total protein extract by phenol/SDS extraction protocol. The difference in the application of the two methods was that extensively washed dry powder of pod tissue were made in Method A, whereas that crude extract were prepared Method B. Extracted proteins were examined using one-dimensional electrophoresis (1-D). Results show that each extraction method isolated a unique subset of cocoa pod proteome. Principal component analysis showed little variation in the data obtained using Method A, while that in Methods B showed no low reproducibility, thus demonstrating that Method A is a reliable for preparing cocoa pod proteins. The protocol is expected to be applicable to other recalcitrant plant tissues and to be of interest to laboratories involved in plant proteomics analyses. A combination of extraction approaches is recommended for increasing proteome coverage when using gel-based isolation techniques. |
publishDate |
2017 |
dc.date.issued.spa.fl_str_mv |
2017-04-01 |
dc.date.accessioned.spa.fl_str_mv |
2019-07-02T19:54:08Z |
dc.date.available.spa.fl_str_mv |
2019-07-02T19:54:08Z |
dc.type.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/ART |
format |
http://purl.org/coar/resource_type/c_6501 |
status_str |
publishedVersion |
dc.identifier.issn.spa.fl_str_mv |
ISSN: 2323-0118 |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/61091 |
dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/59899/ |
identifier_str_mv |
ISSN: 2323-0118 |
url |
https://repositorio.unal.edu.co/handle/unal/61091 http://bdigital.unal.edu.co/59899/ |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/54598 |
dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica Acta Agronómica |
dc.relation.references.spa.fl_str_mv |
Martínez-Márquez, Ascensión and Morante-Carriel, Jaime Alfredo and Bru-Martínez, Roque (2017) A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod. Acta Agronómica, 66 (2). 248 - 253. ISSN 2323-0118 |
dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
dc.rights.uri.spa.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia - Sede Palmira |
institution |
Universidad Nacional de Colombia |
bitstream.url.fl_str_mv |
https://repositorio.unal.edu.co/bitstream/unal/61091/1/54598-311480-5-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/61091/2/54598-311480-5-PB.pdf.jpg |
bitstream.checksum.fl_str_mv |
6e09a93c43cd2a7ead3ca1da4a3ab5ca d1b8f92f0b382743c769af6be7a3b93c |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
repository.mail.fl_str_mv |
repositorio_nal@unal.edu.co |
_version_ |
1814089442396209152 |