A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod

Cocoa, Theobroma cacao L. is one of the main tropical industrial crops. Cocoa has a very high level of interfering substances, such as polysaccharides and phenolic compounds that could prevent the isolation of suitable protein. Efficient methods of protein extraction are a priority to successfully a...

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Autores:
Martínez-Márquez, Ascensión
Morante-Carriel, Jaime Alfredo
Bru-Martínez, Roque
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/61091
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/61091
http://bdigital.unal.edu.co/59899/
Palabra clave:
55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Cocoa pod
One-dimensional electrophoresis
Phenol/SDS extraction protocols
Proteome analyses
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Martínez-Márquez, Ascensión072ac28e-22c8-438a-bfe1-9715d825d378300Morante-Carriel, Jaime Alfredof78d932e-b00a-4ad3-92e4-1c90eb1bcc60300Bru-Martínez, Roque620a9f4a-87c8-476a-8a9e-1663f2629c973002019-07-02T19:54:08Z2019-07-02T19:54:08Z2017-04-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/61091http://bdigital.unal.edu.co/59899/Cocoa, Theobroma cacao L. is one of the main tropical industrial crops. Cocoa has a very high level of interfering substances, such as polysaccharides and phenolic compounds that could prevent the isolation of suitable protein. Efficient methods of protein extraction are a priority to successfully apply proteomic analyses. We compared and evaluated two methods (A and B) of tissue preparation for total protein extract by phenol/SDS extraction protocol. The difference in the application of the two methods was that extensively washed dry powder of pod tissue were made in Method A, whereas that crude extract were prepared Method B. Extracted proteins were examined using one-dimensional electrophoresis (1-D). Results show that each extraction method isolated a unique subset of cocoa pod proteome. Principal component analysis showed little variation in the data obtained using Method A, while that in Methods B showed no low reproducibility, thus demonstrating that Method A is a reliable for preparing cocoa pod proteins. The protocol is expected to be applicable to other recalcitrant plant tissues and to be of interest to laboratories involved in plant proteomics analyses. A combination of extraction approaches is recommended for increasing proteome coverage when using gel-based isolation techniques.application/pdfspaUniversidad Nacional de Colombia - Sede Palmirahttps://revistas.unal.edu.co/index.php/acta_agronomica/article/view/54598Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaMartínez-Márquez, Ascensión and Morante-Carriel, Jaime Alfredo and Bru-Martínez, Roque (2017) A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod. Acta Agronómica, 66 (2). 248 - 253. ISSN 2323-011855 Ciencias de la tierra / Earth sciences and geology63 Agricultura y tecnologías relacionadas / AgricultureCocoa podOne-dimensional electrophoresisPhenol/SDS extraction protocolsProteome analysesA comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) podArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL54598-311480-5-PB.pdfapplication/pdf13107306https://repositorio.unal.edu.co/bitstream/unal/61091/1/54598-311480-5-PB.pdf6e09a93c43cd2a7ead3ca1da4a3ab5caMD51THUMBNAIL54598-311480-5-PB.pdf.jpg54598-311480-5-PB.pdf.jpgGenerated Thumbnailimage/jpeg7585https://repositorio.unal.edu.co/bitstream/unal/61091/2/54598-311480-5-PB.pdf.jpgd1b8f92f0b382743c769af6be7a3b93cMD52unal/61091oai:repositorio.unal.edu.co:unal/610912024-04-16 23:38:41.476Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
title A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
spellingShingle A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Cocoa pod
One-dimensional electrophoresis
Phenol/SDS extraction protocols
Proteome analyses
title_short A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
title_full A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
title_fullStr A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
title_full_unstemmed A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
title_sort A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod
dc.creator.fl_str_mv Martínez-Márquez, Ascensión
Morante-Carriel, Jaime Alfredo
Bru-Martínez, Roque
dc.contributor.author.spa.fl_str_mv Martínez-Márquez, Ascensión
Morante-Carriel, Jaime Alfredo
Bru-Martínez, Roque
dc.subject.ddc.spa.fl_str_mv 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
topic 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
Cocoa pod
One-dimensional electrophoresis
Phenol/SDS extraction protocols
Proteome analyses
dc.subject.proposal.spa.fl_str_mv Cocoa pod
One-dimensional electrophoresis
Phenol/SDS extraction protocols
Proteome analyses
description Cocoa, Theobroma cacao L. is one of the main tropical industrial crops. Cocoa has a very high level of interfering substances, such as polysaccharides and phenolic compounds that could prevent the isolation of suitable protein. Efficient methods of protein extraction are a priority to successfully apply proteomic analyses. We compared and evaluated two methods (A and B) of tissue preparation for total protein extract by phenol/SDS extraction protocol. The difference in the application of the two methods was that extensively washed dry powder of pod tissue were made in Method A, whereas that crude extract were prepared Method B. Extracted proteins were examined using one-dimensional electrophoresis (1-D). Results show that each extraction method isolated a unique subset of cocoa pod proteome. Principal component analysis showed little variation in the data obtained using Method A, while that in Methods B showed no low reproducibility, thus demonstrating that Method A is a reliable for preparing cocoa pod proteins. The protocol is expected to be applicable to other recalcitrant plant tissues and to be of interest to laboratories involved in plant proteomics analyses. A combination of extraction approaches is recommended for increasing proteome coverage when using gel-based isolation techniques.
publishDate 2017
dc.date.issued.spa.fl_str_mv 2017-04-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T19:54:08Z
dc.date.available.spa.fl_str_mv 2019-07-02T19:54:08Z
dc.type.spa.fl_str_mv Artículo de revista
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status_str publishedVersion
dc.identifier.issn.spa.fl_str_mv ISSN: 2323-0118
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/61091
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/59899/
identifier_str_mv ISSN: 2323-0118
url https://repositorio.unal.edu.co/handle/unal/61091
http://bdigital.unal.edu.co/59899/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/54598
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica
Acta Agronómica
dc.relation.references.spa.fl_str_mv Martínez-Márquez, Ascensión and Morante-Carriel, Jaime Alfredo and Bru-Martínez, Roque (2017) A comparison of tissue preparation methods for protein extraction of cocoa (Theobroma cacao L.) pod. Acta Agronómica, 66 (2). 248 - 253. ISSN 2323-0118
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Palmira
institution Universidad Nacional de Colombia
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