Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos

ilustraciones, diagramas, fotografías

Autores:
Piracoca Robles, Mónica Johana
Tipo de recurso:
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
OAI Identifier:
oai:repositorio.unal.edu.co:unal/84381
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/84381
https://repositorio.unal.edu.co/
Palabra clave:
Industrias alimenticias
Cocoa
Pectina
Food industry and trade
Pectin
Mucílago de cacao
Pectina
Carbohidratos
Extracción asistida por ultrasonido
Aprovechamiento
Cocoa mucilage
Pectin
carbohydrates
Ultrasound
Use
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_6c17ba135d31730f70c7d0d50b15e825
oai_identifier_str oai:repositorio.unal.edu.co:unal/84381
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos
dc.title.translated.eng.fl_str_mv Characterization of cocoa mucilage (Theobroma Cacao L., clone TSH 565) as a source of pectin and sugars for use in the food industry
title Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos
spellingShingle Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos
Industrias alimenticias
Cocoa
Pectina
Food industry and trade
Pectin
Mucílago de cacao
Pectina
Carbohidratos
Extracción asistida por ultrasonido
Aprovechamiento
Cocoa mucilage
Pectin
carbohydrates
Ultrasound
Use
title_short Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos
title_full Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos
title_fullStr Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos
title_full_unstemmed Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos
title_sort Caracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentos
dc.creator.fl_str_mv Piracoca Robles, Mónica Johana
dc.contributor.advisor.none.fl_str_mv Palomeque Forero, Liliam Alexandra
dc.contributor.author.none.fl_str_mv Piracoca Robles, Mónica Johana
dc.contributor.researchgroup.spa.fl_str_mv Grupo de investigación en Química de alimentos de la Facultad de Ciencias Departamento de Química
dc.subject.lemb.spa.fl_str_mv Industrias alimenticias
Cocoa
Pectina
topic Industrias alimenticias
Cocoa
Pectina
Food industry and trade
Pectin
Mucílago de cacao
Pectina
Carbohidratos
Extracción asistida por ultrasonido
Aprovechamiento
Cocoa mucilage
Pectin
carbohydrates
Ultrasound
Use
dc.subject.lemb.eng.fl_str_mv Food industry and trade
Pectin
dc.subject.proposal.spa.fl_str_mv Mucílago de cacao
Pectina
Carbohidratos
Extracción asistida por ultrasonido
Aprovechamiento
dc.subject.proposal.eng.fl_str_mv Cocoa mucilage
Pectin
carbohydrates
Ultrasound
Use
description ilustraciones, diagramas, fotografías
publishDate 2022
dc.date.issued.none.fl_str_mv 2022
dc.date.accessioned.none.fl_str_mv 2023-07-31T20:28:32Z
dc.date.available.none.fl_str_mv 2023-07-31T20:28:32Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/84381
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/84381
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.relation.references.spa.fl_str_mv Abarca, D., Martínez, R., Muñoz, J. J., Torres, M. P., & Vargas, G. (2010). Residuos de Café, Cacao y Cladodio de Tuna: Fuentes Promisorias de Fibra Dietaria. Revista Tecnológica - ESPOL, 23(2). http://rte.espol.edu.ec/index.php/tecnologica/article/view/57
Adetunji, L. R., Adekunle, A., Orsat, V., & Raghavan, V. (2017). Advances in the pectin production process using novel extraction techniques: A review. In Food Hydrocolloids (Vol. 62, pp. 239–250). Elsevier. https://doi.org/10.1016/j.foodhyd.2016.08.015
Afoakwa, E. O., Kongor, J. E., Takrama, J. F., & Budu, A. S. (2013). Changes in acidification, sugars and mineral composition of cocoa pulp during fermentation of pulp pre-conditioned. International Food Research Journal 2, 20(3), 1215–1222.
Ramírez, L. M. G. (2020). Diseño de un producto horneado adicionado con fibra dietaria proveniente de la cascarilla de cacao (Theobroma cacao L.). Universidad Nacional de Colombia .
Riveros-Gomez, M., Zalazar-García, D., Mut, I., Torres-Sciancalepore, R., Fabani, M. P., Rodriguez, R., & Mazza, G. (2022a). Multiobjective Optimization and Implementation of a Biorefinery Production Scheme for Sustainable Extraction of Pectin from Quince Biowaste. ACS Engineering Au. https://doi.org/10.1021/acsengineeringau.2c00018
Roini, C., Asbirayani Limatahu, N., Mulya Hartati, T., & Sundari. (2019). Characterization of Cocoa Pulp (Theobroma cacao L) from South Halmahera as an Alternative Feedstock for Bioethanol Production. IOP Conference Series: Earth and Environmental Science, 276(1). https://doi.org/10.1088/1755-1315/276/1/012038
Saavedra-Sanabria, O. L., Durán, D., Cabezas, J., Hernández, I., Blanco-Tirado, C., & Combariza, M. Y. (2021). Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate. Carbohydrate Polymers, 274. https://doi.org/10.1016/j.carbpol.2021.118645
Sánchez-Camargo, A. del P., Gutiérrez, L. F., Vargas, S. M., Martinez-Correa, H. A., Parada-Alfonso, F., & Narváez-Cuenca, C. E. (2019). Valorisation of mango peel: Proximate composition, supercritical fluid extraction of carotenoids, and application as an antioxidant additive for an edible oil. Journal of Supercritical Fluids, 152. https://doi.org/10.1016/j.supflu.2019.104574
Santos, E. E., Amaro, R. C., Bustamante, C. C. C., Guerra, M. H. A., Soares, L. C., & Froes, R. E. S. (2020). Extraction of pectin from agroindustrial residue with an ecofriendly solvent: use of FTIR and chemometrics to differentiate pectins according to degree of methyl esterification. Food Hydrocolloids, 107. https://doi.org/10.1016/j.foodhyd.2020.105921
Soares, T. F., & Oliveira, M. B. P. P. (2022). Cocoa By-Products: Characterization of Bioactive Compounds and Beneficial Health Effects. In Molecules (Vol. 27, Issue 5). MDPI. https://doi.org/10.3390/molecules27051625
Sriamornsak, P. (2003). Chemistry of Pectin and Its Pharmaceutical Uses : A Review. Silpakorn University International Journal, 3, 206–228.
Thakur, B. R., Singh, R. K., & Handa, A. K. (1997). Chemistry and Uses of Pectin - A Review. In Critical Reviews in Food Science and Nutrition (Vol. 37, Issue 1, pp. 47–73). Taylor and Francis Inc. https://doi.org/10.1080/10408399709527767
Tran, T. T. B., Saifullah, M., Nguyen, N. H., Nguyen, M. H., & Vuong, Q. v. (2021). Comparison of ultrasound-assisted and conventional extraction for recovery of pectin from Gac (Momordica cochinchinensis) pulp. Future Foods, 4. https://doi.org/10.1016/j.fufo.2021.100074
Unidad de Gestión de Riesgos Agropecuarios -UGRA. (2020). Ficha de inteligencia: Cacao.
Vásquez, Z. S., de Carvalho Neto, D. P., Pereira, G. V. M., Vandenberghe, L. P. S., de Oliveira, P. Z., Tiburcio, P. B., Rogez, H. L. G., Góes Neto, A., & Soccol, C. R. (2019a). Biotechnological approaches for cocoa waste management: A review. Waste Management, 90, 72–83. https://doi.org/10.1016/j.wasman.2019.04.030
Vergara-Mendoza, M., Martínez, G. R., Blanco-Tirado, C., & Combariza, M. Y. (2022). Mass Balance and Compositional Analysis of Biomass Outputs from Cacao Fruits. Molecules, 27(12), 3717. https://doi.org/10.3390/molecules27123717
Villa-Uvidia, D. N., Osorio-Rivera, M. Á., & Villacis-Venegas Norma Yolanda. (2020). Extracción, propiedades y beneficios de los mucílagos. Ciencias Técnicas y Aplicadas, 6(2), 503–524. https://doi.org/10.23857/dc.v6i2.1181
Vriesmann, L. C., & de Oliveira Petkowicz, C. L. (2009). Polysaccharides from the pulp of cupuassu (Theobroma grandiflorum): Structural characterization of a pectic fraction. Carbohydrate Polymers, 77(1), 72–79. https://doi.org/10.1016/j.carbpol.2008.12.007
Vriesmann, L. C., & Petkowicz, C. L. O. (2013). Highly acetylated pectin from cacao pod husks (Theobroma cacao L.) forms gel. Food Hydrocolloids, 33(1), 58–65. https://doi.org/10.1016/j.foodhyd.2013.02.010
Vriesmann, L. C., Teófilo, R. F., & Lúcia de Oliveira Petkowicz, C. (2012). Extraction and characterization of pectin from cacao pod husks (Theobroma cacao L.) with citric acid. LWT, 49(1), 108–116. https://doi.org/10.1016/j.lwt.2012.04.018
Wang, M., Huang, B., Fan, C., Zhao, K., Hu, H., Xu, X., Pan, S., & Liu, F. (2016). Characterization and functional properties of mango peel pectin extracted by ultrasound assisted citric acid. International Journal of Biological Macromolecules, 91, 794–803. https://doi.org/10.1016/J.IJBIOMAC.2016.06.011
Yang, J. S., Mu, T. H., & Ma, M. M. (2018). Extraction, structure, and emulsifying properties of pectin from potato pulp. Food Chemistry, 244(October 2017), 197–205. https://doi.org/10.1016/j.foodchem.2017.10.059
Zamora, A., & Ochoa, L. (2020). Cadena productiva del cacao en Colombia
Zannini, D., Santagata, G., Malinconico, M., & Immirzi, B. (2021). PECTIN GREEN EXTRACTION FROM CITRUS WASTE: PREPARATION, CHARACTERIZATION AND FUTURE APPLICATIONS OF PECTIN BIOCOMPOSITES. In S. Consonni & M. Giugliano (Eds.), RECOVERY & FINAL SINKS FOR AN EFFECTIVE WASTE MANAGEMENT (pp. 37–38). www.mater.polimi.it
Aguilera-Angel, E. Y., Espinal-Ruiz, M., & Narváez-Cuenca, C. E. (2018). Pectic polysaccharides with different structural characteristics as inhibitors of pancreatic lipase. Food Hydrocolloids, 83, 229–238. https://doi.org/10.1016/j.foodhyd.2018.05.009
Alava Zambrano, W. A. (2020). Caracterización fisica-quimica del mucílago de cacao (Theobroma cacao L.) con enfasis en los azucares que lo componen
Alonso, D., & Molina, R. (2011). Revisión: Productos Vegetales Como Fuente De Fibra Dietaria En La Industria De Alimentos Vegetable Products As Source of Dietary Fiber in the Food Industry: a Review. Revista Facultad Nacional de Agronomía, 64(1), 6023–6035.
Anvoh, K. Y. B., Zoro Bi, A., & Gnakri, D. (2009). Production and characterization of juice from mucilage of cocoa beans and its transformation into marmalade. Pakistan Journal of Nutrition , 2, 129–133.
AOAC. (2012). Official Method of Analysis: Association of Analytical Chemists . In AOAC Internacional: Vol. 19th ed
Arana, A. A. E., & Rugel Jimenez, E. C. (2017). Propuesta de aprovechamiento del desecho mucílago de cacao en la hacienda Santa Rita
Asgari, K., Labbafi, M., Khodaiyan, F., Kazemi, M., & Hosseini, S. S. (2020). High-methylated pectin from walnut processing wastes as a potential resource: Ultrasound assisted extraction and physicochemical, structural and functional analysis. International Journal of Biological Macromolecules, 152, 1274–1282. https://doi.org/10.1016/j.ijbiomac.2019.10.224
Balladares, C., Chóez-Guaranda, I., García, J., Sosa, D., Pérez, S., González, J. E., Viteri, R., Barragán, A., Quijano-Avilés, M., & Manzano, P. (2016). Physicochemical characterization of Theobroma cacao L. sweatings in Ecuadorian coast. Emirates Journal of Food and Agriculture, 28(10), 741–745. https://doi.org/10.9755/ejfa.2016-02-187
Banco de Desarollo de América Latina (CAF). (2019). OBSERVATORIO DEL CACAO FINO Y DE AROMA PARA AMÉRICA LATINA. http://scioteca.caf.com/bitstream/handle/123456789/1408/Iniciativa Latinoamericana del Cacao-Boletín 5.pdf?sequence=2&isAllowed=y
Banco de Desarrollo de América Latina (CAF). (2020). OBSERVATORIO DEL CACAO FINO Y DE AROMA PARA AMÉRICA LATINA. http://bit.ly/CIAT_CGIAR_
CEDAIT. (2021). Caracterización de las agrocadenas de cacao, flores, carne y leche bovina Una revisión funcional, económico, social y ambiental. (Primera ed). Universidad de Antioquia. https://www.udea.edu.co/wps/wcm/connect/udea/d6e3a3f8-655e-40ef-8aa1-4d7ba432ded6/LIBRO+VERSIO%CC%81N+DEFINITIVA_compressed.pdf?MOD=AJPERES&CVID=nHADxmt
Chan, M. (2021). EL CHOCOLATE DE INCOA DE NESTLÉ UTILIZA RESIDUOS DE FRUTA DE CACAO -. https://bartalks.net/es/nestle-lanza-chocolate-incoa-hecho-con-residuos-de-fruta-de-cacao/amp/?amp
Chan, S. Y., & Choo, W. S. (2013). Effect of extraction conditions on the yield and chemical properties of pectin from cocoa husks. Food Chemistry, 141(4), 3752–3758. https://doi.org/10.1016/j.foodchem.2013.06.097
Chemat, F., Vian, M. A., & Cravotto, G. (2012). Green extraction of natural products: Concept and principles. In International Journal of Molecular Sciences (Vol. 13, Issue 7, pp. 8615–8627). MDPI AG. https://doi.org/10.3390/ijms13078615
Cho, E. H., Jung, H. T., Lee, B. H., Kim, H. S., Rhee, J. K., & Yoo, S. H. (2019). Green process development for apple-peel pectin production by organic acid extraction. Carbohydrate Polymers, 204, 97–103. https://doi.org/10.1016/j.carbpol.2018.09.086
Ciriminna, R., Fidalgo, A., Scurria, A., Ilharco, L. M., & Pagliaro, M. (2022). Pectin: New science and forthcoming applications of the most valued hydrocolloid. In Food Hydrocolloids (Vol. 127). Elsevier B.V. https://doi.org/10.1016/j.foodhyd.2022.107483
da Silva, E. N., da Cruz Ramos, D., Miranda Menezes, L., de Souza, A. O., da Silva Lannes, S. C., & da Silva, M. V. (2014). Nutritional value and antioxidant capacity of “cocoa honey” (Theobroma cacao L.). Food Science and Technology, 34(4), 755–759. https://doi.org/10.1590/1678-457X.6447
Dand, R. (2011). Agronomics of international cocoa production. In The International Cocoa Trade (pp. 23–64). Elsevier. https://doi.org/10.1016/b978-0-85709-125-3.50002-1
Defensoría del Pueblo. (2017). Análisis de la fase inicial de diseño e implementación del Programa Nacional de Sustitución de Cultivos de Uso Ilícito - PNIS.
Departamento Nacional de Planeación (DPN). (2016). Estudio de pérdida y desperdicios de alimentos en Colombia. www.dnp.gov.co
Dias, D. R., Schwan, R. F., Freire, E. S., & dos Santos Serôdio, R. (2007). Elaboration of a fruit wine from cocoa (Theobroma cacao L.) pulp. International Journal of Food Science and Technology, 42(3), 319–329. https://doi.org/10.1111/j.1365-2621.2006.01226.x
Dranca, F., & Oroian, M. (2018). Extraction, purification and characterization of pectin from alternative sources with potential technological applications. In Food Research International (Vol. 113, pp. 327–350). Elsevier Ltd. https://doi.org/10.1016/j.foodres.2018.06.065
Dranca, F., & Oroian, M. (2019). Ultrasound-Assisted Extraction of Pectin from Malus domestica ‘Fălticeni’ Apple Pomace. Processes, 7(8). https://doi.org/10.3390/PR7080488
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28(3), 350–356. https://doi.org/10.1021/ac60111a017
Einhorn-Stoll, U., Kastner, H., & Senge, B. (2012). Comparison of molecular parameters; Materials gelling behaviour of comercial citrus pectins. In P. A. Williams & G. O. Phillips (Eds.), Gums and Stabilisers for the Food Industry 16 (pp. 191–197). The Royal Society of Chemistry . https://books.google.com.co/books?hl=es&lr=&id=FTCfvVCK780C&oi=fnd&pg=PA191&dq=Gel+Break+Measurement+AND+Pectin&ots=sHB0aK6wwb&sig=_JSDTHsKzHuI2kQXp5FP8fK1Mn0&redir_esc=y#v=onepage&q=gel%20breaking&f=false
Endraiyani, V., Ludescher, R. D., Di, R., & Karwe, M. v. (2017). Total Phenolics and Antioxidant Capacity of Cocoa Pulp: Processing and Storage Study. Journal of Food Processing and Preservation, 41(4). https://doi.org/10.1111/jfpp.13029
Espinal Ruiz, M. (2016). Efecto de la pectina sobre la actividad de algunas enzimas digestivas y la digestión de lípidos.
European Food Safety Authority (EFSA). (2019). Technical Report on the notification of pulp from Theobroma cacao L. as a traditional food from a third country pursuant to Article 14 of Regulation (EU) 2015/2283. EFSA Supporting Publications, 16(11). https://doi.org/10.2903/sp.efsa.2019.EN-1724
FEDECACAO. (2021, November 3). Año cacaotero 2020-2021, el de mayor producción de cacao en la historia de Colombia. https://www.fedecacao.com.co/post/a%C3%B1o-cacaotero-2020-2021-el-de-mayor-producci%C3%B3n-de-cacao-en-la-historia-de-colombia
Ferreira Ardila, S. (2007). Pectinas: aislamiento, caracterización y producción a partir de frutas tropicales y de los residuos de su procesamiento industrial. (1st ed.). Universidad Nacional de Colombia .
Festring Daniel, Kusche Beatrice, & Batista Vieira Josélio. (2019). Chocolate products, ingredients, processes and uses (Patent No. EP3520621A1). European Patent Office. https://patents.google.com/patent/EP3520621A1/en?oq=EP+3520621A1
Filho, L. B. S., Coelho, R. C., Muniz, E. C., & Barbosa, H. de S. (2022). Optimization of pectin extraction using response surface methodology: A bibliometric analysis. Carbohydrate Polymer Technologies and Applications, 4.
Freitas de Oliveira, C., Giordani, D., Lutckemier, R., Gurak, P. D., Cladera-Olivera, F., & Ferreira Marczak, L. D. (2016). Extraction of pectin from passion fruit peel assisted by ultrasound. LWT, 71, 110–115. https://doi.org/10.1016/j.lwt.2016.03.027
Garcés P, E. (2009). Diseño y fabricación de un sistema prototipo que permita la separación de las almendras y el mucílago
García-Montoya, C., & Penagos-Gómez, C. E. (2011). EL ENTORNO COMERCIAL DE LA PECTINA EN LA INDUSTRIA ALIMENTARIA ANTIOQUEÑA. Escuela de Ingeniería de Antioquia
Gnanasambandam, R., & Proctor, A. (2000). Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy. Food Chemistry , 68, 327–332. www.elsevier.com/locate/foodchem
Grassino, A. N., Barba, F. J., Brnčić, M., Lorenzo, J. M., Lucini, L., & Brnčić, S. R. (2018). Analytical tools used for the identification and quantification of pectin extracted from plant food matrices, wastes and by-products: A review. In Food Chemistry (Vol. 266, pp. 47–55). Elsevier Ltd. https://doi.org/10.1016/j.foodchem.2018.05.105
Gray, J., & ILSI Europe. (2006). Dietary fibre : definition, analysis, physiology & health. ILSI Europe
Guirlanda, C. P., da Silva, G. G., & Takahashi, J. A. (2021). Cocoa honey: Agro-industrial waste or underutilized cocoa by-product? Future Foods, 4(April), 100061. https://doi.org/10.1016/j.fufo.2021.100061
Harholt, J., Suttangkakul, A., & Vibe Scheller, H. (2010). Biosynthesis of Pectin . Plant Physiology, 153, 384–395
Hielscher. (2021). Higher Pectin Yields with Ultrasonic Extraction. https://www.hielscher.com/higher-pectin-yields-with-ultrasonic-extraction.htm
ICCO. (2021). Industria del Chocolate . https://www.icco.org/chocolate-industry/
INCONTEC. (2017). NTC 1779 “Método para determinar azúcares reductores, totales en meladura y mieles de caña, después de la hidrólisis por el procedimiento Lane y Eynon a volumen constante” .
Jafari, F., Khodaiyan, F., Kiani, H., & Hosseini, S. S. (2017). Pectin from carrot pomace: Optimization of extraction and physicochemical properties. Carbohydrate Polymers, 157, 1315–1322. https://doi.org/10.1016/j.carbpol.2016.11.013
Jayeola, C. O., Adebowale, B. A., Yahaya, L. E., Ogunwolu, S. O., & Olubamiwa, O. (2018). Chapter 18. Production of Bioactive Compounds From Waste. In Therapeutic, Probiotic, and Unconventional Foods (pp. 340–354).
Kassem, I. A. A., Joshua Ashaolu, T., Kamel, R., Elkasabgy, N. A., Afifi, S. M., & Farag, M. A. (2021). Mucilage as a functional food hydrocolloid: Ongoing and potential applications in prebiotics and nutraceuticals. In Food and Function (Vol. 12, Issue 11, pp. 4738–4748). Royal Society of Chemistry. https://doi.org/10.1039/d1fo00438g
Kongor, J. E., Hinneh, M., de Walle, D. van, Afoakwa, E. O., Boeckx, P., & Dewettinck, K. (2016). Factors influencing quality variation in cocoa (Theobroma cacao) bean flavour profile - A review. In Food Research International (Vol. 82, pp. 44–52). Elsevier Ltd. https://doi.org/10.1016/j.foodres.2016.01.012
Lai, J. C. H., Mahesan, D., Abdul Samat, N. A. S. binti, & Baini, R. (2022). Characterization and optimization of extracted pectin from unripe banana and mango fruit peels. Materials Today: Proceedings, 65, 3020–3029. https://doi.org/10.1016/j.matpr.2022.03.580
Lima, Lí. J. R., Almeida, M. H., Rob Nout, M. J., & Zwietering, M. H. (2011). Theobroma cacao L., “the food of the gods”: Quality determinants of commercial cocoa beans, with particular reference to the impact of fermentation. Critical Reviews in Food Science and Nutrition, 51(8), 731–761. https://doi.org/10.1080/10408391003799913
Ma, S., Yu, S. J., Zheng, X. L., Wang, X. X., Bao, Q. D., & Guo, X. M. (2013). Extraction, characterization and spontaneous emulsifying properties of pectin from sugar beet pulp. Carbohydrate Polymers, 98(1), 750–753. https://doi.org/10.1016/j.carbpol.2013.06.042
Manrique, G. D., & Lajolo, F. M. (2002). FT-IR spectroscopy as a tool for measuring degree of methyl esterification in pectins isolated from ripening papaya fruit. In Postharvest Biology and Technology (Vol. 25). www.elsevier.com/locate/postharvbio
Martínez, R., Torres, P., Meneses, M. A., Figueroa, J. G., Pérez-Álvarez, J. A., & Viuda-Martos, M. (2012). Chemical, technological and in vitro antioxidant properties of cocoa (Theobroma cacao L.) co-products. Food Research International, 49(1), 39–45. https://doi.org/10.1016/j.foodres.2012.08.005
Marzano Moncayo, S. M. (2018). Diseño de un proceso industrial para la obtención de mermelada a partir de mucílago de cacao ".
Masuko, T., Minami, A., Iwasaki, N., Majima, T., Nishimura, S. I., & Lee, Y. C. (2005). Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Analytical Biochemistry, 339(1), 69–72. https://doi.org/10.1016/j.ab.2004.12.001
Matos-Chamorro, A., & Chambilla-Mamani, E. (2010). Importancia de la Fibra Dietética, sus Propiedades Funcionales en la Alimentación Humana y en la Industria Alimentaria. Revista de Investigación En Ciencia y Tecnología de Alimentos , 1(1), 4–17.
Meersman, E., Struyf, N., Kyomugasho, C., Jamsazzadeh Kermani, Z., Santiago, J. S., Baert, E., Hemdane, S., Vrancken, G., Verstrepen, K. J., Courtin, C. M., Hendrickx, M., & Steensels, J. (2017). Characterization and Degradation of Pectic Polysaccharides in Cocoa Pulp. Journal of Agricultural and Food Chemistry, 65(44), 9726–9734. https://doi.org/10.1021/acs.jafc.7b03854
Mellinas, C., Ramos, M., Jiménez, A., & Garrigós, M. C. (2020). Recent trends in the use of pectin from agro-waste residues as a natural-based biopolymer for food packaging applications. In Materials (Vol. 13, Issue 3). MDPI AG. https://doi.org/10.3390/ma13030673
Méndez-Sánchez, A. F., Pérez-Trejo, L., & Mercado, A. M. P. (2010). Determinación de la viscosidad de fluidos newtonianos y no newtonianos (una revisión del viscosímetro de Couette) I. INTRODUCCIÓN. Am. J. Phys. Educ, 4(1). http://www.journal.lapen.org.mx
Ministerio de Agricultura y Desarrollo Rural. (2021). Cadena de valor de Cacao. Direccion de Cadenas Agrícolas y Forestales.
Molina Soler, D. E. (2016). Extracción de pectina de frutos amazónicos mediante un proceso asistido por microondas.
Mollea, C., Chiampo, F., & Conti, R. (2008). Extraction and characterization of pectins from cocoa husks: A preliminary study. Food Chemistry, 107(3), 1353–1356. https://doi.org/10.1016/j.foodchem.2007.09.006
Monsoor, M. A., Kalapathy, U., & Proctor, A. (2001). Analytical nutritional and clinical methods section Determination of polygalacturonic acid content in pectin extracts by di€use re¯ectance Fourier transform infrared spectroscopy. Food Chemistry , 74, 233–238. www.elsevier.com/locate/foodchem
Nieto, K. H., Mendoza, N. V., & Campos, R. (2020). Cocoa By-products. In Food Wastes and By‐products: Nutraceutical and Health Potential (pp. 373–411). https://doi.org/10.1002/9781119534167.ch13
Nigam, P. S., & Singh, A. (2014). Cocoa and Coffee Fermentations. In Encyclopedia of Food Microbiology: Second Edition (Second Edi, Vol. 1). Elsevier. https://doi.org/10.1016/B978-0-12-384730-0.00074-4
Nunes, C. S. O., da Silva, M. L. C., Camilloto, G. P., Machado, B. A. S., Hodel, K. V. S., Koblitz, M. G. B., Carvalho, G. B. M., & Uetanabaro, A. P. T. (2020). Potential Applicability of Cocoa Pulp (Theobroma cacao L) as an Adjunct for Beer Production. The Scientific World Journal, 2020, 16–18. https://doi.org/10.1155/2020/3192585
Oddoye, E. O. K., Agyente-Badu, C. K., & Gyedu-Akoto, E. (2013). Cocoa and its by-products: Identification and utilization. In Chocolate in Health and Nutrition (pp. 23–37). Humana Press Inc. https://doi.org/10.1007/978-1-61779-803-0_3
Oliveira, T. Í. S., Rosa, M. F., Cavalcante, F. L., Pereira, P. H. F., Moates, G. K., Wellner, N., Mazzetto, S. E., Waldron, K. W., & Azeredo, H. M. C. (2016). Optimization of pectin extraction from banana peels with citric acid by using response surface methodology. Food Chemistry, 198, 113–118. https://doi.org/10.1016/j.foodchem.2015.08.080
Organización de la Naciones Unidas (ONU). (2022). Objetivos de Desarrollo Sostenible . https://colombia.un.org/es/sdgs
Organización Internacional del Cacao (ICCO). (2010). Cultivo de Cacao -. https://www.icco.org/growing-cocoa/
Ospina, K. G. C. (2016). Estudio de la interacción de hidrocoloides empleados en alimentos y su efecto en las propiedades reológicas y de textura sensorial e instrumental. Universidad Nacional de Colombia .
Pangestu, R., Amanah, S., Juanssilfero, A. B., Yopi, & Perwitasari, U. (2020). Response surface methodology for microwave-assisted extraction of pectin from cocoa pod husk (Theobroma cacao) mediated by oxalic acid. Journal of Food Measurement and Characterization, 14(4), 2126–2133. https://doi.org/10.1007/s11694-020-00459-4
Peng, X. Y., Mu, T. H., Zhang, M., Sun, H. N., Chen, J. W., & Yu, M. (2015). Optimisation of production yield by ultrasound-/microwave-assisted acid method and functional property of pectin from sugar beet pulp. International Journal of Food Science and Technology, 50(3), 758–765. https://doi.org/10.1111/ijfs.12678
Perea V, A., Martínez G, N., Aranzazu H, F., & Cadena C, T. (2013). Caracteristicas de calidad del cacao de Colombia, Catalogo de 26 Cultivares. Universidad industrial de Santander, Federacion Nacional de cacaoteros.
Pereira, G. V. de M., Soccol, V. T., & Soccol, C. R. (2016). Current state of research on cocoa and coffee fermentations. Current Opinion in Food Science, 7, 50–57. https://doi.org/10.1016/j.cofs.2015.11.001
Pereira, P., Oliveira, T. Í. S., Rosa, M. F., Cavalcante, F. L., Moates, G. K., Wellner, N., Waldron, K. W., & Azeredo, H. M. C. (2016). Pectin extraction from pomegranate peels with citric acid. International Journal of Biological Macromolecules, 88, 373–379. https://doi.org/10.1016/j.ijbiomac.2016.03.074
Pettipher, G. L. (1986). Analysis of Cocoa Pulp and the Formulation of a Standardised Artificial Cocoa Pulp Medium. In J. Sci. Food Agric (Vol. 37).
Picot-Allain, M. C. N., Ramasawmy, B., & Emmambux, M. N. (2022). Extraction, Characterisation, and Application of Pectin from Tropical and Sub-Tropical Fruits: A Review. In Food Reviews International (Vol. 38, Issue 3, pp. 282–312). Taylor and Francis Ltd. https://doi.org/10.1080/87559129.2020.1733008
Polanco-Lugo, E., Martínez-Castillo, J. I., Cuevas-Bernardino, J. C., González-Flores, T., Valdez-Ojeda, R., Pacheco, N., & Ayora-Talavera, T. (2019). Citrus pectin obtained by ultrasound-assisted extraction: Physicochemical, structural, rheological and functional properties. CYTA - Journal of Food, 17(1), 463–471. https://doi.org/10.1080/19476337.2019.1600036
Priyangini, F., Walde, S. G., & Chidambaram, R. (2018). Extraction optimization of pectin from cocoa pod husks (Theobroma cacao L.) with ascorbic acid using response surface methodology. Carbohydrate Polymers, 202, 497–503. https://doi.org/10.1016/j.carbpol.2018.08.103
Pueraria, C., Schwan, R., & Magalhães, K. (2012). New cocoa pulp-based kefir beverages: Microbiological, chemical composition andsensory analysis. Food Research International, 48(2), 634–640. https://www.sciencedirect.com/science/article/pii/S0963996912001974#!
Quijano-Ortega, N. Y. (2020). Evaluación de espectroscopía FTIR-ATR, colorimetría triestímulo y análisis de imagen como herramientas para la determinación de carotenoides en ahuyama. Universidad Nacional de Colombia .
Quintero Valbuena, M. A. (2016a). Obtención y caracterización de hidrocoloides a partir de residuos de la industria frutícola. Universidad Nacional de Colombia
Sánchez-Camargo, A. del P., Gutiérrez, L. F., Vargas, S. M., Martinez-Correa, H. A., Parada-Alfonso, F., & Narváez-Cuenca, C. E. (2019). Valorisation of mango peel: Proximate composition, supercritical fluid extraction of carotenoids, and application as an antioxidant additive for an edible oil. Journal of Supercritical Fluids, 152. https://doi.org/10.1016/j.supflu.2019.104574
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.extent.spa.fl_str_mv 114 páginas
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv Bogotá - Ciencias Agrarias - Maestría en Ciencia y Tecnología de Alimentos
dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias Agrarias
dc.publisher.place.spa.fl_str_mv Bogotá,Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/84381/1/license.txt
https://repositorio.unal.edu.co/bitstream/unal/84381/2/1024543556.2023.pdf
https://repositorio.unal.edu.co/bitstream/unal/84381/3/1024543556.2023.pdf.jpg
bitstream.checksum.fl_str_mv eb34b1cf90b7e1103fc9dfd26be24b4a
615832cb3753d2a15e342d0c36320313
bf2633c097d70be2cab789d5c84ffeeb
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv repositorio_nal@unal.edu.co
_version_ 1814089308927164416
spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Palomeque Forero, Liliam Alexandraca36db8fec0058cf73315571a21efd31Piracoca Robles, Mónica Johanac86323f910fb4332b837c694b05ff7d3Grupo de investigación en Química de alimentos de la Facultad de Ciencias Departamento de Química2023-07-31T20:28:32Z2023-07-31T20:28:32Z2022https://repositorio.unal.edu.co/handle/unal/84381Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografíasEl mucílago o pulpa de cacao es un subproducto obtenido antes del proceso de fermentación. Ha sido utilizado como materia prima para la fabricación de mermeladas, bebidas alcohólicas, entre otros. Sin embargo, aunque está principalmente constituido por azúcar y fibra dietaria, se han realizado pocos estudios sobre el contenido y la caracterización de la pectina extraída del mucílago de cacao para su uso en la industria de alimentos. En este estudio se obtuvo la pulpa a partir del clon TSH 565 de San Vicente del Chucurí. Se realizó el análisis proximal, la determinación de fibra dietaria total, bajo el método AOAC 985.29. Adicionalmente, se realizó la cuantificación de sacarosa, maltosa, glucosa y fructosa utilizando HPLC. Finalmente se realizó la extracción de pectina asistida por ultrasonido utilizando una solución de ácido cítrico como solvente, variando las condiciones de pH, tiempo de extracción y la relación masa-solvente (m/v). El mucílago de cacao tiene aproximadamente 15,23 ± 0,08 % de azúcares totales. El contenido de sacarosa es de 6,32 ± 0,24 g / 100 g de mucílago, seguido de fructosa 4,36 ± 0,11g/100 g de mucílago, glucosa 4,02 ± 0,09 g/ 100 g de mucílago y, maltosa 0,96 ± 0,02 g/ 100 g de mucílago, cuantificado a 40 °C. La extracción de pectina óptima se obtuvo bajo las condiciones de pH 2, tiempo de extracción 10 min y la relación m/v 1:10, obteniendo un rendimiento 9,33 g / 100 g de mucílago seco, pectina que tiene un contenido de 67,97 % de ácido galacturónico (GA) y 78,38 % grado de esterificación (DE). Las pectinas extraídas formaron geles a pH ácido y alto contenido de sacarosa. Mediante esta investigación se realizó una contribución tecnológica para el posible aprovechamiento de mucílago como fuente de pectina empleando una solución de ácido cítrico como solvente “verde “ y se proponen algunas alternativas para su aprovechamiento. La pectina es un compuesto de interés en la industria de alimentos y contribuiría al crecimiento económico de los cultivadores de cacao. Adicionalmente a partir del contenido de sacarosa y su degradación térmica, se puede realizar una propuesta de procesamiento, donde se evite la degradación y se garantice la calidad del producto. (Texto tomado de la fuente)The mucilage or cocoa pulp is a by-product obtained before the fermentation process. It is using as a raw material for manufacture of jams, alcoholic beverages, among others. However, although it is form of sugar and dietary fiber, few studies have been carried out on the content and characterization of the pectin extracted from cocoa mucilage for use in the food industry. In this study, the pulp was obtained from the clone TSH 565 from San Vicente del Chucurí. The proximal analysis, the determination of total dietary fiber, was carried out under the AOAC 985.29 method. Additionally, the quantification of sucrose, maltose, glucose and fructose was performed using HPLC. Finally, ultrasound-assisted pectin extraction was carried out using a citric acid solution as a solvent, varying the pH conditions, extraction time and the mass-solvent ratio (m/v). Cocoa mucilage has approximately 15.23 ± 0.08% total sugars. The sucrose content is 6.32 ± 0.24 g/100 g of mucilage, followed by fructose 4.36 ± 0.11g/100 g of mucilage, glucose 4.02 ± 0.09 g/100 g of mucilage. and, maltose 0.96 ± 0.02 g/100 g of mucilage, quantified at 40 °C. The optimal pectin extraction was obtained under the conditions of pH 2, extraction time 10 min and the m/v ratio 1:10, obtaining a yield of 9.33 g / 100 g of dry mucilage, pectin that has a content of 67 .97% galacturonic acid (GA) and 78.38% degree of esterification (DE). The extracted pectins formed gels at acidic pH and high sucrose content. Through this research, a technological contribution was made for the possible use of mucilage as a source of pectin using a citric acid solution as a "green" solvent and some alternatives for its use are proposed. Pectin is a compound of interest in the food industry and would contribute to the economic growth of cocoa farmers. Additionally, based on the sucrose content and its thermal degradation, a processing proposal can be made, where degradation is avoided and product quality is guaranteed.MaestríaDesarrollo de nuevos productos e ingredientes alimentarios114 páginasUniversidad Nacional de ColombiaBogotá - Ciencias Agrarias - Maestría en Ciencia y Tecnología de AlimentosFacultad de Ciencias AgrariasBogotá,ColombiaUniversidad Nacional de Colombia - Sede BogotáCaracterización del mucílago de cacao ( Theobroma Cacao L., clon TSH 565) como fuente de pectina y azúcares para el aprovechamiento en la industria de alimentosCharacterization of cocoa mucilage (Theobroma Cacao L., clone TSH 565) as a source of pectin and sugars for use in the food industryTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbarca, D., Martínez, R., Muñoz, J. J., Torres, M. P., & Vargas, G. (2010). Residuos de Café, Cacao y Cladodio de Tuna: Fuentes Promisorias de Fibra Dietaria. Revista Tecnológica - ESPOL, 23(2). http://rte.espol.edu.ec/index.php/tecnologica/article/view/57Adetunji, L. R., Adekunle, A., Orsat, V., & Raghavan, V. (2017). Advances in the pectin production process using novel extraction techniques: A review. In Food Hydrocolloids (Vol. 62, pp. 239–250). Elsevier. https://doi.org/10.1016/j.foodhyd.2016.08.015Afoakwa, E. O., Kongor, J. E., Takrama, J. F., & Budu, A. S. (2013). Changes in acidification, sugars and mineral composition of cocoa pulp during fermentation of pulp pre-conditioned. International Food Research Journal 2, 20(3), 1215–1222.Ramírez, L. M. G. (2020). Diseño de un producto horneado adicionado con fibra dietaria proveniente de la cascarilla de cacao (Theobroma cacao L.). Universidad Nacional de Colombia .Riveros-Gomez, M., Zalazar-García, D., Mut, I., Torres-Sciancalepore, R., Fabani, M. P., Rodriguez, R., & Mazza, G. (2022a). Multiobjective Optimization and Implementation of a Biorefinery Production Scheme for Sustainable Extraction of Pectin from Quince Biowaste. ACS Engineering Au. https://doi.org/10.1021/acsengineeringau.2c00018Roini, C., Asbirayani Limatahu, N., Mulya Hartati, T., & Sundari. (2019). Characterization of Cocoa Pulp (Theobroma cacao L) from South Halmahera as an Alternative Feedstock for Bioethanol Production. IOP Conference Series: Earth and Environmental Science, 276(1). https://doi.org/10.1088/1755-1315/276/1/012038Saavedra-Sanabria, O. L., Durán, D., Cabezas, J., Hernández, I., Blanco-Tirado, C., & Combariza, M. Y. (2021). Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate. Carbohydrate Polymers, 274. https://doi.org/10.1016/j.carbpol.2021.118645Sánchez-Camargo, A. del P., Gutiérrez, L. F., Vargas, S. M., Martinez-Correa, H. A., Parada-Alfonso, F., & Narváez-Cuenca, C. E. (2019). Valorisation of mango peel: Proximate composition, supercritical fluid extraction of carotenoids, and application as an antioxidant additive for an edible oil. Journal of Supercritical Fluids, 152. https://doi.org/10.1016/j.supflu.2019.104574Santos, E. E., Amaro, R. C., Bustamante, C. C. C., Guerra, M. H. A., Soares, L. C., & Froes, R. E. S. (2020). Extraction of pectin from agroindustrial residue with an ecofriendly solvent: use of FTIR and chemometrics to differentiate pectins according to degree of methyl esterification. Food Hydrocolloids, 107. https://doi.org/10.1016/j.foodhyd.2020.105921Soares, T. F., & Oliveira, M. B. P. P. (2022). Cocoa By-Products: Characterization of Bioactive Compounds and Beneficial Health Effects. In Molecules (Vol. 27, Issue 5). MDPI. https://doi.org/10.3390/molecules27051625Sriamornsak, P. (2003). Chemistry of Pectin and Its Pharmaceutical Uses : A Review. Silpakorn University International Journal, 3, 206–228.Thakur, B. R., Singh, R. K., & Handa, A. K. (1997). Chemistry and Uses of Pectin - A Review. In Critical Reviews in Food Science and Nutrition (Vol. 37, Issue 1, pp. 47–73). Taylor and Francis Inc. https://doi.org/10.1080/10408399709527767Tran, T. T. B., Saifullah, M., Nguyen, N. H., Nguyen, M. H., & Vuong, Q. v. (2021). Comparison of ultrasound-assisted and conventional extraction for recovery of pectin from Gac (Momordica cochinchinensis) pulp. Future Foods, 4. https://doi.org/10.1016/j.fufo.2021.100074Unidad de Gestión de Riesgos Agropecuarios -UGRA. (2020). Ficha de inteligencia: Cacao.Vásquez, Z. S., de Carvalho Neto, D. P., Pereira, G. V. M., Vandenberghe, L. P. S., de Oliveira, P. Z., Tiburcio, P. B., Rogez, H. L. G., Góes Neto, A., & Soccol, C. R. (2019a). Biotechnological approaches for cocoa waste management: A review. Waste Management, 90, 72–83. https://doi.org/10.1016/j.wasman.2019.04.030Vergara-Mendoza, M., Martínez, G. R., Blanco-Tirado, C., & Combariza, M. Y. (2022). Mass Balance and Compositional Analysis of Biomass Outputs from Cacao Fruits. Molecules, 27(12), 3717. https://doi.org/10.3390/molecules27123717Villa-Uvidia, D. N., Osorio-Rivera, M. Á., & Villacis-Venegas Norma Yolanda. (2020). Extracción, propiedades y beneficios de los mucílagos. Ciencias Técnicas y Aplicadas, 6(2), 503–524. https://doi.org/10.23857/dc.v6i2.1181Vriesmann, L. C., & de Oliveira Petkowicz, C. L. (2009). Polysaccharides from the pulp of cupuassu (Theobroma grandiflorum): Structural characterization of a pectic fraction. Carbohydrate Polymers, 77(1), 72–79. https://doi.org/10.1016/j.carbpol.2008.12.007Vriesmann, L. C., & Petkowicz, C. L. O. (2013). Highly acetylated pectin from cacao pod husks (Theobroma cacao L.) forms gel. Food Hydrocolloids, 33(1), 58–65. https://doi.org/10.1016/j.foodhyd.2013.02.010Vriesmann, L. C., Teófilo, R. F., & Lúcia de Oliveira Petkowicz, C. (2012). Extraction and characterization of pectin from cacao pod husks (Theobroma cacao L.) with citric acid. LWT, 49(1), 108–116. https://doi.org/10.1016/j.lwt.2012.04.018Wang, M., Huang, B., Fan, C., Zhao, K., Hu, H., Xu, X., Pan, S., & Liu, F. (2016). Characterization and functional properties of mango peel pectin extracted by ultrasound assisted citric acid. International Journal of Biological Macromolecules, 91, 794–803. https://doi.org/10.1016/J.IJBIOMAC.2016.06.011Yang, J. S., Mu, T. H., & Ma, M. M. (2018). Extraction, structure, and emulsifying properties of pectin from potato pulp. Food Chemistry, 244(October 2017), 197–205. https://doi.org/10.1016/j.foodchem.2017.10.059Zamora, A., & Ochoa, L. (2020). Cadena productiva del cacao en ColombiaZannini, D., Santagata, G., Malinconico, M., & Immirzi, B. (2021). PECTIN GREEN EXTRACTION FROM CITRUS WASTE: PREPARATION, CHARACTERIZATION AND FUTURE APPLICATIONS OF PECTIN BIOCOMPOSITES. In S. Consonni & M. Giugliano (Eds.), RECOVERY & FINAL SINKS FOR AN EFFECTIVE WASTE MANAGEMENT (pp. 37–38). www.mater.polimi.itAguilera-Angel, E. Y., Espinal-Ruiz, M., & Narváez-Cuenca, C. E. (2018). Pectic polysaccharides with different structural characteristics as inhibitors of pancreatic lipase. Food Hydrocolloids, 83, 229–238. https://doi.org/10.1016/j.foodhyd.2018.05.009Alava Zambrano, W. A. (2020). Caracterización fisica-quimica del mucílago de cacao (Theobroma cacao L.) con enfasis en los azucares que lo componenAlonso, D., & Molina, R. (2011). Revisión: Productos Vegetales Como Fuente De Fibra Dietaria En La Industria De Alimentos Vegetable Products As Source of Dietary Fiber in the Food Industry: a Review. Revista Facultad Nacional de Agronomía, 64(1), 6023–6035.Anvoh, K. Y. B., Zoro Bi, A., & Gnakri, D. (2009). Production and characterization of juice from mucilage of cocoa beans and its transformation into marmalade. Pakistan Journal of Nutrition , 2, 129–133.AOAC. (2012). Official Method of Analysis: Association of Analytical Chemists . In AOAC Internacional: Vol. 19th edArana, A. A. E., & Rugel Jimenez, E. C. (2017). Propuesta de aprovechamiento del desecho mucílago de cacao en la hacienda Santa RitaAsgari, K., Labbafi, M., Khodaiyan, F., Kazemi, M., & Hosseini, S. S. (2020). High-methylated pectin from walnut processing wastes as a potential resource: Ultrasound assisted extraction and physicochemical, structural and functional analysis. International Journal of Biological Macromolecules, 152, 1274–1282. https://doi.org/10.1016/j.ijbiomac.2019.10.224Balladares, C., Chóez-Guaranda, I., García, J., Sosa, D., Pérez, S., González, J. E., Viteri, R., Barragán, A., Quijano-Avilés, M., & Manzano, P. (2016). Physicochemical characterization of Theobroma cacao L. sweatings in Ecuadorian coast. Emirates Journal of Food and Agriculture, 28(10), 741–745. https://doi.org/10.9755/ejfa.2016-02-187Banco de Desarollo de América Latina (CAF). (2019). OBSERVATORIO DEL CACAO FINO Y DE AROMA PARA AMÉRICA LATINA. http://scioteca.caf.com/bitstream/handle/123456789/1408/Iniciativa Latinoamericana del Cacao-Boletín 5.pdf?sequence=2&isAllowed=yBanco de Desarrollo de América Latina (CAF). (2020). OBSERVATORIO DEL CACAO FINO Y DE AROMA PARA AMÉRICA LATINA. http://bit.ly/CIAT_CGIAR_CEDAIT. (2021). Caracterización de las agrocadenas de cacao, flores, carne y leche bovina Una revisión funcional, económico, social y ambiental. (Primera ed). Universidad de Antioquia. https://www.udea.edu.co/wps/wcm/connect/udea/d6e3a3f8-655e-40ef-8aa1-4d7ba432ded6/LIBRO+VERSIO%CC%81N+DEFINITIVA_compressed.pdf?MOD=AJPERES&CVID=nHADxmtChan, M. (2021). EL CHOCOLATE DE INCOA DE NESTLÉ UTILIZA RESIDUOS DE FRUTA DE CACAO -. https://bartalks.net/es/nestle-lanza-chocolate-incoa-hecho-con-residuos-de-fruta-de-cacao/amp/?ampChan, S. Y., & Choo, W. S. (2013). Effect of extraction conditions on the yield and chemical properties of pectin from cocoa husks. Food Chemistry, 141(4), 3752–3758. https://doi.org/10.1016/j.foodchem.2013.06.097Chemat, F., Vian, M. A., & Cravotto, G. (2012). Green extraction of natural products: Concept and principles. In International Journal of Molecular Sciences (Vol. 13, Issue 7, pp. 8615–8627). MDPI AG. https://doi.org/10.3390/ijms13078615Cho, E. H., Jung, H. T., Lee, B. H., Kim, H. S., Rhee, J. K., & Yoo, S. H. (2019). Green process development for apple-peel pectin production by organic acid extraction. Carbohydrate Polymers, 204, 97–103. https://doi.org/10.1016/j.carbpol.2018.09.086Ciriminna, R., Fidalgo, A., Scurria, A., Ilharco, L. M., & Pagliaro, M. (2022). Pectin: New science and forthcoming applications of the most valued hydrocolloid. In Food Hydrocolloids (Vol. 127). Elsevier B.V. https://doi.org/10.1016/j.foodhyd.2022.107483da Silva, E. N., da Cruz Ramos, D., Miranda Menezes, L., de Souza, A. O., da Silva Lannes, S. C., & da Silva, M. V. (2014). Nutritional value and antioxidant capacity of “cocoa honey” (Theobroma cacao L.). Food Science and Technology, 34(4), 755–759. https://doi.org/10.1590/1678-457X.6447Dand, R. (2011). Agronomics of international cocoa production. In The International Cocoa Trade (pp. 23–64). Elsevier. https://doi.org/10.1016/b978-0-85709-125-3.50002-1Defensoría del Pueblo. (2017). Análisis de la fase inicial de diseño e implementación del Programa Nacional de Sustitución de Cultivos de Uso Ilícito - PNIS.Departamento Nacional de Planeación (DPN). (2016). Estudio de pérdida y desperdicios de alimentos en Colombia. www.dnp.gov.coDias, D. R., Schwan, R. F., Freire, E. S., & dos Santos Serôdio, R. (2007). Elaboration of a fruit wine from cocoa (Theobroma cacao L.) pulp. International Journal of Food Science and Technology, 42(3), 319–329. https://doi.org/10.1111/j.1365-2621.2006.01226.xDranca, F., & Oroian, M. (2018). Extraction, purification and characterization of pectin from alternative sources with potential technological applications. In Food Research International (Vol. 113, pp. 327–350). Elsevier Ltd. https://doi.org/10.1016/j.foodres.2018.06.065Dranca, F., & Oroian, M. (2019). Ultrasound-Assisted Extraction of Pectin from Malus domestica ‘Fălticeni’ Apple Pomace. Processes, 7(8). https://doi.org/10.3390/PR7080488Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28(3), 350–356. https://doi.org/10.1021/ac60111a017Einhorn-Stoll, U., Kastner, H., & Senge, B. (2012). Comparison of molecular parameters; Materials gelling behaviour of comercial citrus pectins. In P. A. Williams & G. O. Phillips (Eds.), Gums and Stabilisers for the Food Industry 16 (pp. 191–197). The Royal Society of Chemistry . https://books.google.com.co/books?hl=es&lr=&id=FTCfvVCK780C&oi=fnd&pg=PA191&dq=Gel+Break+Measurement+AND+Pectin&ots=sHB0aK6wwb&sig=_JSDTHsKzHuI2kQXp5FP8fK1Mn0&redir_esc=y#v=onepage&q=gel%20breaking&f=falseEndraiyani, V., Ludescher, R. D., Di, R., & Karwe, M. v. (2017). Total Phenolics and Antioxidant Capacity of Cocoa Pulp: Processing and Storage Study. Journal of Food Processing and Preservation, 41(4). https://doi.org/10.1111/jfpp.13029Espinal Ruiz, M. (2016). Efecto de la pectina sobre la actividad de algunas enzimas digestivas y la digestión de lípidos.European Food Safety Authority (EFSA). (2019). Technical Report on the notification of pulp from Theobroma cacao L. as a traditional food from a third country pursuant to Article 14 of Regulation (EU) 2015/2283. EFSA Supporting Publications, 16(11). https://doi.org/10.2903/sp.efsa.2019.EN-1724FEDECACAO. (2021, November 3). Año cacaotero 2020-2021, el de mayor producción de cacao en la historia de Colombia. https://www.fedecacao.com.co/post/a%C3%B1o-cacaotero-2020-2021-el-de-mayor-producci%C3%B3n-de-cacao-en-la-historia-de-colombiaFerreira Ardila, S. (2007). Pectinas: aislamiento, caracterización y producción a partir de frutas tropicales y de los residuos de su procesamiento industrial. (1st ed.). Universidad Nacional de Colombia .Festring Daniel, Kusche Beatrice, & Batista Vieira Josélio. (2019). Chocolate products, ingredients, processes and uses (Patent No. EP3520621A1). European Patent Office. https://patents.google.com/patent/EP3520621A1/en?oq=EP+3520621A1Filho, L. B. S., Coelho, R. C., Muniz, E. C., & Barbosa, H. de S. (2022). Optimization of pectin extraction using response surface methodology: A bibliometric analysis. Carbohydrate Polymer Technologies and Applications, 4.Freitas de Oliveira, C., Giordani, D., Lutckemier, R., Gurak, P. D., Cladera-Olivera, F., & Ferreira Marczak, L. D. (2016). Extraction of pectin from passion fruit peel assisted by ultrasound. LWT, 71, 110–115. https://doi.org/10.1016/j.lwt.2016.03.027Garcés P, E. (2009). Diseño y fabricación de un sistema prototipo que permita la separación de las almendras y el mucílagoGarcía-Montoya, C., & Penagos-Gómez, C. E. (2011). EL ENTORNO COMERCIAL DE LA PECTINA EN LA INDUSTRIA ALIMENTARIA ANTIOQUEÑA. Escuela de Ingeniería de AntioquiaGnanasambandam, R., & Proctor, A. (2000). Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy. Food Chemistry , 68, 327–332. www.elsevier.com/locate/foodchemGrassino, A. N., Barba, F. J., Brnčić, M., Lorenzo, J. M., Lucini, L., & Brnčić, S. R. (2018). Analytical tools used for the identification and quantification of pectin extracted from plant food matrices, wastes and by-products: A review. In Food Chemistry (Vol. 266, pp. 47–55). Elsevier Ltd. https://doi.org/10.1016/j.foodchem.2018.05.105Gray, J., & ILSI Europe. (2006). Dietary fibre : definition, analysis, physiology & health. ILSI EuropeGuirlanda, C. P., da Silva, G. G., & Takahashi, J. A. (2021). Cocoa honey: Agro-industrial waste or underutilized cocoa by-product? Future Foods, 4(April), 100061. https://doi.org/10.1016/j.fufo.2021.100061Harholt, J., Suttangkakul, A., & Vibe Scheller, H. (2010). Biosynthesis of Pectin . Plant Physiology, 153, 384–395Hielscher. (2021). Higher Pectin Yields with Ultrasonic Extraction. https://www.hielscher.com/higher-pectin-yields-with-ultrasonic-extraction.htmICCO. (2021). Industria del Chocolate . https://www.icco.org/chocolate-industry/INCONTEC. (2017). NTC 1779 “Método para determinar azúcares reductores, totales en meladura y mieles de caña, después de la hidrólisis por el procedimiento Lane y Eynon a volumen constante” .Jafari, F., Khodaiyan, F., Kiani, H., & Hosseini, S. S. (2017). Pectin from carrot pomace: Optimization of extraction and physicochemical properties. Carbohydrate Polymers, 157, 1315–1322. https://doi.org/10.1016/j.carbpol.2016.11.013Jayeola, C. O., Adebowale, B. A., Yahaya, L. E., Ogunwolu, S. O., & Olubamiwa, O. (2018). Chapter 18. Production of Bioactive Compounds From Waste. In Therapeutic, Probiotic, and Unconventional Foods (pp. 340–354).Kassem, I. A. A., Joshua Ashaolu, T., Kamel, R., Elkasabgy, N. A., Afifi, S. M., & Farag, M. A. (2021). Mucilage as a functional food hydrocolloid: Ongoing and potential applications in prebiotics and nutraceuticals. In Food and Function (Vol. 12, Issue 11, pp. 4738–4748). Royal Society of Chemistry. https://doi.org/10.1039/d1fo00438gKongor, J. E., Hinneh, M., de Walle, D. van, Afoakwa, E. O., Boeckx, P., & Dewettinck, K. (2016). Factors influencing quality variation in cocoa (Theobroma cacao) bean flavour profile - A review. In Food Research International (Vol. 82, pp. 44–52). Elsevier Ltd. https://doi.org/10.1016/j.foodres.2016.01.012Lai, J. C. H., Mahesan, D., Abdul Samat, N. A. S. binti, & Baini, R. (2022). Characterization and optimization of extracted pectin from unripe banana and mango fruit peels. Materials Today: Proceedings, 65, 3020–3029. https://doi.org/10.1016/j.matpr.2022.03.580Lima, Lí. J. R., Almeida, M. H., Rob Nout, M. J., & Zwietering, M. H. (2011). Theobroma cacao L., “the food of the gods”: Quality determinants of commercial cocoa beans, with particular reference to the impact of fermentation. Critical Reviews in Food Science and Nutrition, 51(8), 731–761. https://doi.org/10.1080/10408391003799913Ma, S., Yu, S. J., Zheng, X. L., Wang, X. X., Bao, Q. D., & Guo, X. M. (2013). Extraction, characterization and spontaneous emulsifying properties of pectin from sugar beet pulp. Carbohydrate Polymers, 98(1), 750–753. https://doi.org/10.1016/j.carbpol.2013.06.042Manrique, G. D., & Lajolo, F. M. (2002). FT-IR spectroscopy as a tool for measuring degree of methyl esterification in pectins isolated from ripening papaya fruit. In Postharvest Biology and Technology (Vol. 25). www.elsevier.com/locate/postharvbioMartínez, R., Torres, P., Meneses, M. A., Figueroa, J. G., Pérez-Álvarez, J. A., & Viuda-Martos, M. (2012). Chemical, technological and in vitro antioxidant properties of cocoa (Theobroma cacao L.) co-products. Food Research International, 49(1), 39–45. https://doi.org/10.1016/j.foodres.2012.08.005Marzano Moncayo, S. M. (2018). Diseño de un proceso industrial para la obtención de mermelada a partir de mucílago de cacao ".Masuko, T., Minami, A., Iwasaki, N., Majima, T., Nishimura, S. I., & Lee, Y. C. (2005). Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Analytical Biochemistry, 339(1), 69–72. https://doi.org/10.1016/j.ab.2004.12.001Matos-Chamorro, A., & Chambilla-Mamani, E. (2010). Importancia de la Fibra Dietética, sus Propiedades Funcionales en la Alimentación Humana y en la Industria Alimentaria. Revista de Investigación En Ciencia y Tecnología de Alimentos , 1(1), 4–17.Meersman, E., Struyf, N., Kyomugasho, C., Jamsazzadeh Kermani, Z., Santiago, J. S., Baert, E., Hemdane, S., Vrancken, G., Verstrepen, K. J., Courtin, C. M., Hendrickx, M., & Steensels, J. (2017). Characterization and Degradation of Pectic Polysaccharides in Cocoa Pulp. Journal of Agricultural and Food Chemistry, 65(44), 9726–9734. https://doi.org/10.1021/acs.jafc.7b03854Mellinas, C., Ramos, M., Jiménez, A., & Garrigós, M. C. (2020). Recent trends in the use of pectin from agro-waste residues as a natural-based biopolymer for food packaging applications. In Materials (Vol. 13, Issue 3). MDPI AG. https://doi.org/10.3390/ma13030673Méndez-Sánchez, A. F., Pérez-Trejo, L., & Mercado, A. M. P. (2010). Determinación de la viscosidad de fluidos newtonianos y no newtonianos (una revisión del viscosímetro de Couette) I. INTRODUCCIÓN. Am. J. Phys. Educ, 4(1). http://www.journal.lapen.org.mxMinisterio de Agricultura y Desarrollo Rural. (2021). Cadena de valor de Cacao. Direccion de Cadenas Agrícolas y Forestales.Molina Soler, D. E. (2016). Extracción de pectina de frutos amazónicos mediante un proceso asistido por microondas.Mollea, C., Chiampo, F., & Conti, R. (2008). Extraction and characterization of pectins from cocoa husks: A preliminary study. Food Chemistry, 107(3), 1353–1356. https://doi.org/10.1016/j.foodchem.2007.09.006Monsoor, M. A., Kalapathy, U., & Proctor, A. (2001). Analytical nutritional and clinical methods section Determination of polygalacturonic acid content in pectin extracts by di€use re¯ectance Fourier transform infrared spectroscopy. Food Chemistry , 74, 233–238. www.elsevier.com/locate/foodchemNieto, K. H., Mendoza, N. V., & Campos, R. (2020). Cocoa By-products. In Food Wastes and By‐products: Nutraceutical and Health Potential (pp. 373–411). https://doi.org/10.1002/9781119534167.ch13Nigam, P. S., & Singh, A. (2014). Cocoa and Coffee Fermentations. In Encyclopedia of Food Microbiology: Second Edition (Second Edi, Vol. 1). Elsevier. https://doi.org/10.1016/B978-0-12-384730-0.00074-4Nunes, C. S. O., da Silva, M. L. C., Camilloto, G. P., Machado, B. A. S., Hodel, K. V. S., Koblitz, M. G. B., Carvalho, G. B. M., & Uetanabaro, A. P. T. (2020). Potential Applicability of Cocoa Pulp (Theobroma cacao L) as an Adjunct for Beer Production. The Scientific World Journal, 2020, 16–18. https://doi.org/10.1155/2020/3192585Oddoye, E. O. K., Agyente-Badu, C. K., & Gyedu-Akoto, E. (2013). Cocoa and its by-products: Identification and utilization. In Chocolate in Health and Nutrition (pp. 23–37). Humana Press Inc. https://doi.org/10.1007/978-1-61779-803-0_3Oliveira, T. Í. S., Rosa, M. F., Cavalcante, F. L., Pereira, P. H. F., Moates, G. K., Wellner, N., Mazzetto, S. E., Waldron, K. W., & Azeredo, H. M. C. (2016). Optimization of pectin extraction from banana peels with citric acid by using response surface methodology. Food Chemistry, 198, 113–118. https://doi.org/10.1016/j.foodchem.2015.08.080Organización de la Naciones Unidas (ONU). (2022). Objetivos de Desarrollo Sostenible . https://colombia.un.org/es/sdgsOrganización Internacional del Cacao (ICCO). (2010). Cultivo de Cacao -. https://www.icco.org/growing-cocoa/Ospina, K. G. C. (2016). Estudio de la interacción de hidrocoloides empleados en alimentos y su efecto en las propiedades reológicas y de textura sensorial e instrumental. Universidad Nacional de Colombia .Pangestu, R., Amanah, S., Juanssilfero, A. B., Yopi, & Perwitasari, U. (2020). Response surface methodology for microwave-assisted extraction of pectin from cocoa pod husk (Theobroma cacao) mediated by oxalic acid. Journal of Food Measurement and Characterization, 14(4), 2126–2133. https://doi.org/10.1007/s11694-020-00459-4Peng, X. Y., Mu, T. H., Zhang, M., Sun, H. N., Chen, J. W., & Yu, M. (2015). Optimisation of production yield by ultrasound-/microwave-assisted acid method and functional property of pectin from sugar beet pulp. International Journal of Food Science and Technology, 50(3), 758–765. https://doi.org/10.1111/ijfs.12678Perea V, A., Martínez G, N., Aranzazu H, F., & Cadena C, T. (2013). Caracteristicas de calidad del cacao de Colombia, Catalogo de 26 Cultivares. Universidad industrial de Santander, Federacion Nacional de cacaoteros.Pereira, G. V. de M., Soccol, V. T., & Soccol, C. R. (2016). Current state of research on cocoa and coffee fermentations. Current Opinion in Food Science, 7, 50–57. https://doi.org/10.1016/j.cofs.2015.11.001Pereira, P., Oliveira, T. Í. S., Rosa, M. F., Cavalcante, F. L., Moates, G. K., Wellner, N., Waldron, K. W., & Azeredo, H. M. C. (2016). Pectin extraction from pomegranate peels with citric acid. International Journal of Biological Macromolecules, 88, 373–379. https://doi.org/10.1016/j.ijbiomac.2016.03.074Pettipher, G. L. (1986). Analysis of Cocoa Pulp and the Formulation of a Standardised Artificial Cocoa Pulp Medium. In J. Sci. Food Agric (Vol. 37).Picot-Allain, M. C. N., Ramasawmy, B., & Emmambux, M. N. (2022). Extraction, Characterisation, and Application of Pectin from Tropical and Sub-Tropical Fruits: A Review. In Food Reviews International (Vol. 38, Issue 3, pp. 282–312). Taylor and Francis Ltd. https://doi.org/10.1080/87559129.2020.1733008Polanco-Lugo, E., Martínez-Castillo, J. I., Cuevas-Bernardino, J. C., González-Flores, T., Valdez-Ojeda, R., Pacheco, N., & Ayora-Talavera, T. (2019). Citrus pectin obtained by ultrasound-assisted extraction: Physicochemical, structural, rheological and functional properties. CYTA - Journal of Food, 17(1), 463–471. https://doi.org/10.1080/19476337.2019.1600036Priyangini, F., Walde, S. G., & Chidambaram, R. (2018). Extraction optimization of pectin from cocoa pod husks (Theobroma cacao L.) with ascorbic acid using response surface methodology. Carbohydrate Polymers, 202, 497–503. https://doi.org/10.1016/j.carbpol.2018.08.103Pueraria, C., Schwan, R., & Magalhães, K. (2012). New cocoa pulp-based kefir beverages: Microbiological, chemical composition andsensory analysis. Food Research International, 48(2), 634–640. https://www.sciencedirect.com/science/article/pii/S0963996912001974#!Quijano-Ortega, N. Y. (2020). Evaluación de espectroscopía FTIR-ATR, colorimetría triestímulo y análisis de imagen como herramientas para la determinación de carotenoides en ahuyama. Universidad Nacional de Colombia .Quintero Valbuena, M. A. (2016a). Obtención y caracterización de hidrocoloides a partir de residuos de la industria frutícola. Universidad Nacional de ColombiaSánchez-Camargo, A. del P., Gutiérrez, L. F., Vargas, S. M., Martinez-Correa, H. A., Parada-Alfonso, F., & Narváez-Cuenca, C. E. (2019). Valorisation of mango peel: Proximate composition, supercritical fluid extraction of carotenoids, and application as an antioxidant additive for an edible oil. Journal of Supercritical Fluids, 152. https://doi.org/10.1016/j.supflu.2019.104574Industrias alimenticiasCocoaPectinaFood industry and tradePectinMucílago de cacaoPectinaCarbohidratosExtracción asistida por ultrasonidoAprovechamientoCocoa mucilagePectincarbohydratesUltrasoundUseLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84381/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1024543556.2023.pdf1024543556.2023.pdfTesis de Maestría en Ciencia y Tecnología de Alimentosapplication/pdf2746438https://repositorio.unal.edu.co/bitstream/unal/84381/2/1024543556.2023.pdf615832cb3753d2a15e342d0c36320313MD52THUMBNAIL1024543556.2023.pdf.jpg1024543556.2023.pdf.jpgGenerated Thumbnailimage/jpeg5908https://repositorio.unal.edu.co/bitstream/unal/84381/3/1024543556.2023.pdf.jpgbf2633c097d70be2cab789d5c84ffeebMD53unal/84381oai:repositorio.unal.edu.co:unal/843812023-08-16 23:03:37.753Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.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