Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido
ilustraciones, tablas
- Autores:
-
Velásquez Quintero, Carolina
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/80747
- Palabra clave:
- 660 - Ingeniería química
Fermentación
Fermentation
Enzimas
Enzymes
Biotecnología
Fermentación en estado sólido
Hongos de la podredumbre blanca
Enzimas ligninolíticas
Lacasa
Extracción enzimática
Purificación e inmovilización enzimática
Colorantes sintéticos
Solid-state fermentation
Ligninolytic enzymes
Laccase
Enzymatic extraction
Enzymatic purification and immobilization
Synthetic dyes
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido |
| dc.title.translated.eng.fl_str_mv |
Production, purification and immobilization of laccase obtained from an optimized solid-state fermentation process |
| title |
Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido |
| spellingShingle |
Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido 660 - Ingeniería química Fermentación Fermentation Enzimas Enzymes Biotecnología Fermentación en estado sólido Hongos de la podredumbre blanca Enzimas ligninolíticas Lacasa Extracción enzimática Purificación e inmovilización enzimática Colorantes sintéticos Solid-state fermentation Ligninolytic enzymes Laccase Enzymatic extraction Enzymatic purification and immobilization Synthetic dyes |
| title_short |
Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido |
| title_full |
Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido |
| title_fullStr |
Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido |
| title_full_unstemmed |
Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido |
| title_sort |
Producción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólido |
| dc.creator.fl_str_mv |
Velásquez Quintero, Carolina |
| dc.contributor.advisor.none.fl_str_mv |
Hormaza Anaguano, Angelina Ruiz Villadiego, Orlando Simon |
| dc.contributor.author.none.fl_str_mv |
Velásquez Quintero, Carolina |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Síntesis, Reactividad y Transformación de Compuestos Orgánicos, Sirytcor |
| dc.subject.ddc.spa.fl_str_mv |
660 - Ingeniería química |
| topic |
660 - Ingeniería química Fermentación Fermentation Enzimas Enzymes Biotecnología Fermentación en estado sólido Hongos de la podredumbre blanca Enzimas ligninolíticas Lacasa Extracción enzimática Purificación e inmovilización enzimática Colorantes sintéticos Solid-state fermentation Ligninolytic enzymes Laccase Enzymatic extraction Enzymatic purification and immobilization Synthetic dyes |
| dc.subject.lemb.none.fl_str_mv |
Fermentación Fermentation Enzimas Enzymes Biotecnología |
| dc.subject.proposal.spa.fl_str_mv |
Fermentación en estado sólido Hongos de la podredumbre blanca Enzimas ligninolíticas Lacasa Extracción enzimática Purificación e inmovilización enzimática Colorantes sintéticos |
| dc.subject.proposal.eng.fl_str_mv |
Solid-state fermentation Ligninolytic enzymes Laccase Enzymatic extraction Enzymatic purification and immobilization Synthetic dyes |
| description |
ilustraciones, tablas |
| publishDate |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2021-11-30T15:39:43Z |
| dc.date.available.none.fl_str_mv |
2021-11-30T15:39:43Z |
| dc.date.issued.none.fl_str_mv |
2021-11-29 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
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Text |
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http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/80747 |
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Universidad Nacional de Colombia |
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Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/80747 https://repositorio.unal.edu.co/ |
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Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Hormaza Anaguano, Angelina70779d78f865ae48c9e652716219e98e600Ruiz Villadiego, Orlando Simonc04face915a5523f79dee380ee442ad7600Velásquez Quintero, Carolina9be927e5528d01beb62f946ea9b6ad99Grupo de Investigación en Síntesis, Reactividad y Transformación de Compuestos Orgánicos, Sirytcor2021-11-30T15:39:43Z2021-11-30T15:39:43Z2021-11-29https://repositorio.unal.edu.co/handle/unal/80747Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, tablasLas enzimas ligninolíticas, llamadas así por su capacidad de oxidar el polímero de la lignina, han cobrado importancia en el campo de la biotecnología en vista de la habilidad que demuestran para oxidar a su vez otro tipo de moléculas orgánicas estructuralmente más sencillas. Esta característica ha potencializado indudablemente su implementación en diversas aplicaciones industriales entre las que se destaca la deslignificación de la pulpa en el procesamiento del papel, el aprovechamiento de residuos agroindustriales para la generación de biocombustibles o enmiendas orgánicas y el tratamiento de efluentes contaminados con compuestos recalcitrantes, tales como los colorantes sintéticos, entre otras. La lacasa, particularmente la proveniente de los conocidos hongos de podredumbre blanca (HPB), pertenece a este grupo de enzimas ligninolíticas y es de especial interés debido a que es producida en gran cantidad y de manera extracelular. Bajo este contexto, el grupo de investigación en Síntesis, Reactividad y Transformación de Compuestos Orgánicos (SIRYTCOR) ha desarrollado una línea de investigación enfocada en la producción de enzimas ligninolíticas mediante fermentación en estado sólido (FES). Así, el presente estudio buscó fortalecer dicha línea investigativa a través de la evaluación de las condiciones más adecuadas para la producción, extracción y cuantificación de lacasa obtenida del proceso de FES sobre un soporte orgánico. Adicionalmente, reconociendo algunas de las limitaciones encontradas en la perspectiva de emplear la lacasa a una mayor escala, se evaluó la purificación e inmovilización de esta enzima y se llevó a cabo un estudio en el que se estimó su capacidad para decolorar un tinte sintético. En principio, se evaluó la producción de lacasa a partir de las cuatro especies de HPB Trametes pubescens, Phanaerochaete chrysosporium, Pleurotus ostreatus y Pleurotus pulmonarius durante un periodo de 7, 10 y 13 días de FES sobre el residuo agrícola tusa de maíz. Pleurotus ostreatus fue seleccionada para llevar a cabo la optimización de las condiciones fermentativas mediante un diseño central compuesto, del cual se estableció que la producción de lacasa se maximiza utilizando el extracto de malta como fuente de carbono, a una concentración de 18.2 g/L; el extracto de levadura como fuente de nitrógeno, ajustando la proporción carbono-nitrógeno a un valor de 5.15:1; el tween 80 como inductor, a una concentración de 0.17% v/v; y el contenido de humedad en 85.0%. Como etapa siguiente, se implementaron diversos diseños factoriales que permitieron seleccionar al buffer acetato de sodio 50 mM, a pH de 6.00 y pH de 4.00, como mejores condiciones para llevar a cabo los procesos de extracción y cuantificación enzimática, respectivamente. Una vez concluida esta fase, se realizó un seguimiento diario de la cinética de actividad enzimática y actividad específica durante 18 días; este estudio permitió concluir que la lacasa producida bajo las condiciones previamente seleccionadas, alcanza su primer pico de actividad al octavo día de fermentación. Con respecto a la actividad enzimática alcanzada, cabe señalar que se obtuvo un incremento de seis veces con respecto al valor reportado al inicio de la investigación (8.6 ± 0.8 U/gss), como resultado de la optimización de las condiciones fermentativas, así como del estudio de los procesos de extracción y cuantificación, y la construcción de las curvas cinéticas. Asimismo, se evaluó la purificación del extracto crudo obtenido, seguido de la inmovilización del extracto purificado. Para la purificación, se evaluaron dos metodologías consecutivas: precipitación con sulfato de amonio y separación por sistemas bifásicos acuosos. Por su parte, para la inmovilización se evaluó una metodología estándar que permitió la síntesis de micropartículas de quitosano funcionalizadas con glutaraldehído. El sistema final, resultante de la precipitación con sulfato de amonio a una concentración de saturación del 70% y la inmovilización de acuerdo con el método enunciado, se aplicó en la decoloración del tinte rojo allura AC (RA), cuyo uso se extiende a industrias como la textil y alimentaria en sus procesos de tinción. En esta última sección, se incluyeron evaluaciones comparativas con varios sistemas, encontrando los mejores resultados del proceso decolorativo con una lacasa comercial, el extracto crudo y el extracto precipitado respectivamente. (Texto tomado de la fuente)igninolytic enzymes, named for their ability to oxidase the lignin polymer, have gained importance in the field of biotechnology because of their demonstrated ability to also oxidase other types of structurally simpler organic molecules. This feature has undoubtedly promoted their implementation in multiple industrial applications, highlighting pulp delignification in paper processing, agro-industrial waste use for the generation of biofuels and organic amendments, and treatment of effluents contaminated with recalcitrant compounds as synthetic dyes, among others. Laccase, particularly from the well-known white-rot fungi (WRF), belongs to this group of ligninolytic enzymes and is especially important because it is produced in large amounts and in an extracellular way. In this context, the research group on Synthesis, Reactivity and Transformation of Organic Compounds (SIRYTCOR for its acronym in Spanish) has developed a research line focused on ligninolytic enzymes production through solid-state fermentation (SSF). Thus, the present study aimed at strengthening that research line by evaluating the most suitable conditions for the production, extraction and quantification of laccase, which was obtained from the SSF process onto an organic support. Furthermore, taking into account some of the limitations found in the perspective of using laccase on a larger scale, the purification and immobilization of this enzyme were evaluated, and a study to determine its capability to decolorize a synthetic dye was carried out. In the first place, laccase production by the four WRF Trametes pubescens, Phanaerochaete chrysosporium, Pleurotus ostreatus and Pleurotus pulmonarius was evaluated during 7, 10 and 13 days of a SSF onto the agricultural by-product corncob. Pleurotus ostreatus was selected to carry out an optimization of the fermentative conditions through a central composite design. It was found that laccase production is maximized using malt extract as carbon source (18.2 g L-1), yeast extract as nitrogen source fitting carbon-nitrogen ratio at 5.15:1, tween 80 as inducer (0.17 % v v-1) and a moisture content of 85%. Subsequently, several factorial designs allowed to select the 50 mM sodium acetate buffer pH = 6.00 and pH = 4.00 as the best conditions to carry out the enzymatic extraction and quantification processes, respectively. Once this phase was completed, a study of the kinetics of enzymatic activity and specific activity was performed during 18 days on a daily basis. This study allowed to conclude that the laccase produced under the previously selected conditions reaches its first peak of activity on the 8th day of fermentation. Regarding the enzymatic activity obtained, it is worth mentioning that it was observed a six-fold increase with respect to the value reached at the beginning of the research (8.6 ± 0.8 U/gss), as a result of the optimization of fermentation conditions, as well as the study of the extraction process, quantification process and the construction of kinetic curves. Likewise, the purification of the crude extract obtained was evaluated, followed by the immobilization of the purified extract. The enzymatic purification was carried out through two consecutive methodologies: ammonium sulfate precipitation and aqueous two-phase system. For its part, enzymatic immobilization was performed using a standard methodology that allowed the synthesis of chitosan microparticles functionalized with glutaraldehyde. The final system, resulting from the ammonium sulfate precipitation with 70% of salt saturation and the immobilization according to the stated method, was evaluated in the decolorization of allura red AC, a synthetic dye extensively used in the textile and food industry in their dyeing processes. In this last section, several systems were considered for comparative purposes. The results show that the best decolorative systems were a commercial laccase, the crude extract and the precipitated extract, respectively.MaestríaMagíster en Ciencias - BiotecnologíaBiotecnología ambientalÁrea curricular Biotecnologíaxxiv, 142 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaEscuela de biocienciasFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín660 - Ingeniería químicaFermentaciónFermentationEnzimasEnzymesBiotecnologíaFermentación en estado sólidoHongos de la podredumbre blancaEnzimas ligninolíticasLacasaExtracción enzimáticaPurificación e inmovilización enzimáticaColorantes sintéticosSolid-state fermentationLigninolytic enzymesLaccaseEnzymatic extractionEnzymatic purification and immobilizationSynthetic dyesProducción, purificación e inmovilización de lacasa obtenida a partir de un proceso optimizado de fermentación en estado sólidoProduction, purification and immobilization of laccase obtained from an optimized solid-state fermentation processTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMD. 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August 2016, pp. 1–8, 2017, doi: 10.1016/j.cep.2017.03.009.Universidad Nacional de ColombiaSistema Nacional de Becas de PosgradoEstudiantesInvestigadoresMaestrosLICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/80747/1/license.txt8153f7789df02f0a4c9e079953658ab2MD51ORIGINAL1017236205.2021.pdf1017236205.2021.pdfTesis de Maestría en Ciencias - Biotecnologíaapplication/pdf1701878https://repositorio.unal.edu.co/bitstream/unal/80747/2/1017236205.2021.pdfe383c2fa6c7d140e6ddeb0c80d43d3eaMD52THUMBNAIL1017236205.2021.pdf.jpg1017236205.2021.pdf.jpgGenerated Thumbnailimage/jpeg4534https://repositorio.unal.edu.co/bitstream/unal/80747/3/1017236205.2021.pdf.jpg509b90941952eb29187bcb5a1bccf90cMD53unal/80747oai:repositorio.unal.edu.co:unal/807472023-07-31 23:03:54.326Repositorio Institucional Universidad Nacional de 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