Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento
ilustraciones, fotografías, gráficas, tablas
- Autores:
-
Upegui Porras, Nicolás
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/82134
- Palabra clave:
- 630 - Agricultura y tecnologías relacionadas::636 - Producción animal
Newcastle disease
Poultry - diseases
Poultry virus diseases
Enfermedad de Newcastle
Aves de corral - enfermedades
Virosis en las aves de corral
RT-qPCR
Inhibición de la hemaglutinación
Maíz
Virus de la enfermedad de Newcastle
Vacuna viva
Administración oral en alimento
Corn
Hemagglutination inhibition
Newcastle disease virus
Live attenuated vaccine
qRT-PCR
Oral administration in food
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento |
| dc.title.translated.eng.fl_str_mv |
Viability of Newcastle disease virus vaccine strains in rice or corn and immune response induced in poultry vaccinated via feed |
| title |
Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento |
| spellingShingle |
Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento 630 - Agricultura y tecnologías relacionadas::636 - Producción animal Newcastle disease Poultry - diseases Poultry virus diseases Enfermedad de Newcastle Aves de corral - enfermedades Virosis en las aves de corral RT-qPCR Inhibición de la hemaglutinación Maíz Virus de la enfermedad de Newcastle Vacuna viva Administración oral en alimento Corn Hemagglutination inhibition Newcastle disease virus Live attenuated vaccine qRT-PCR Oral administration in food |
| title_short |
Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento |
| title_full |
Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento |
| title_fullStr |
Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento |
| title_full_unstemmed |
Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento |
| title_sort |
Viabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimento |
| dc.creator.fl_str_mv |
Upegui Porras, Nicolás |
| dc.contributor.advisor.spa.fl_str_mv |
Álvarez Mira, Diana Marcela Gómez Ramírez, Arlen Patricia |
| dc.contributor.author.spa.fl_str_mv |
Upegui Porras, Nicolás |
| dc.subject.ddc.spa.fl_str_mv |
630 - Agricultura y tecnologías relacionadas::636 - Producción animal |
| topic |
630 - Agricultura y tecnologías relacionadas::636 - Producción animal Newcastle disease Poultry - diseases Poultry virus diseases Enfermedad de Newcastle Aves de corral - enfermedades Virosis en las aves de corral RT-qPCR Inhibición de la hemaglutinación Maíz Virus de la enfermedad de Newcastle Vacuna viva Administración oral en alimento Corn Hemagglutination inhibition Newcastle disease virus Live attenuated vaccine qRT-PCR Oral administration in food |
| dc.subject.lemb.eng.fl_str_mv |
Newcastle disease Poultry - diseases Poultry virus diseases |
| dc.subject.lemb.spa.fl_str_mv |
Enfermedad de Newcastle Aves de corral - enfermedades Virosis en las aves de corral |
| dc.subject.proposal.spa.fl_str_mv |
RT-qPCR Inhibición de la hemaglutinación Maíz Virus de la enfermedad de Newcastle Vacuna viva Administración oral en alimento |
| dc.subject.proposal.eng.fl_str_mv |
Corn Hemagglutination inhibition Newcastle disease virus Live attenuated vaccine qRT-PCR Oral administration in food |
| description |
ilustraciones, fotografías, gráficas, tablas |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-08-26T14:04:47Z |
| dc.date.available.none.fl_str_mv |
2022-08-26T14:04:47Z |
| dc.date.issued.none.fl_str_mv |
2022-08 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/82134 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/82134 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
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spa |
| dc.relation.references.spa.fl_str_mv |
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M., Kehler, H., Pasick, J., y Calderón, C. P. (2017). Characterization of Colombian serotype 1 avian paramyxoviruses,. 2008–2010. Virus genes, 53(4), 584-592. https://doi.org/10.1007/s11262-017-1461-z Bossart, K. N., Fusco, D. L., y Broder, C. C. (2013). Paramyxovirus entry. Advances in Experimental Medicine and Biology, 790, 95–127. https://doi.org/10.1007/978-1-4614-7651-1_6 Boumart, Z., Hamdi, J., Daouam, S., Elarkam, A., Tadlaoui, K. O., y El Harrak, M. (2016). Thermal Stability Study of Five Newcastle Disease Attenuated Vaccine Strains. Avian Diseases, 60(4), 779–783. https://doi.org/10.1637/11426-042116-Reg.1 Brown, V. R., y Bevins, S. N. (2017). A review of virulent Newcastle disease viruses in the United States and the role of wild birds in viral persistence and spread. Veterinary Research, 48(1), 1–15. https://doi.org/10.1186/s13567-017-0475-9 Bu, Y. wen, Yang, H. ming, Jin, J. hui, Zhao, J., Xue, J., y Zhang, G. zhong. (2019). 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Methods, 25(4), 402–408. https://doi.org/ 10.1007/s11033-011-1135-1 M Khatun, S Islam, M Ershaduzzaman, HMS Islam, S Yasmin y A Hossen, M. H. (2018). Economic Impact of Newcastle Disease on Village Chickens – A Case of Bangladesh. 1, 358–367. https://doi.org/10.31014/aior.1992.01.03.33 Martinez, J. C. S., Chou, W. K., Berghman, L. R., y Carey, J. B. (2018). Evaluation of the effect of live LaSota Newcastle disease virus vaccine as primary immunization on immune development in broilers. Poultry Science, 97(2), 455–462. https://doi.org/10.3382/ps/pex339 Mebrahtu, K., Teshale, S., Esatu, W., Habte, T., y Gelaye, E. (2018). Evaluation of spray and oral delivery of Newcastle disease I2 vaccine in chicken reared by smallholder farmers in central Ethiopia. BMC Veterinary Research, 14(1), 1–7. https://doi.org/10.1186/s12917-018-1355-x Mengesha, M., Tamir, B., y Dessie, T. (2011). Village chicken constraints and traditional management practices in Jamma District, South Wollo, Ethiopia. Livestock Research for Rural Development, 23(2). Millar, N. S., Chambers, P., y Emmerson, P. T. (1988). Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of Newcastle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains. The Journal of General Virology, 69 ( Pt 3), 613–620. https://doi.org/10.1099/0022-1317-69-3-613. Miller, P. J., Afonso, C. L., El Attrache, J., Dorsey, K. M., Courtney, S. C., Guo, Z., y Kapczynski, D. R. (2013). Effects of Newcastle disease virus vaccine antibodies on the shedding and transmission of challenge viruses. Developmental and Comparative Immunology, 41(4), 505–513. https://doi.org/10.1016/j.dci.2013.06.007 Mossos, N., Peña, E., y Correa, R. (2004). Guía metodológica para la definición y atención de focos de la enfermedad de Newcastle. Grupo Tran. https://www.solla.com/sites/default/files/productos/secciones/adjuntos/Newcastlefocos.pdf Mostafijur, M., Deb, R., y Nooruzzaman, M. (2017). Evaluation of serum antibody titer level against Newcastle disease virus in vaccinated broiler chickens. Tierärztl Prax, 45, 151-158. Musa, U., Abdu, P.A, Mera, U.M., Emmenna, P.E. y Ahmed, M. S. (2010). Vaccination with newcastle disease vaccines strain i2 and lasota in commercial and local chickens in plateau state Nigeria. Nigerian Veterinary Journal, 31, 46–55. Naggar H.M., Madkour M.S., Hussein H.A (2017). Preparation of mucosal nanoparticles and polymer-based inactivated vaccine for Newcastle disease and H9N2 AI viruses, Veterinary World, 10(2): 187-193. https://doi.org/10.14202/vetworld.2017.187-193 Nagai, Y., Hamaguchi, M., y Toyoda, T. (1989). Molecular biology of Newcastle disease virus. Progress in Veterinary Microbiology and Immunology, 5, 16–64. Nasrin, M., Khan, M. Z. I., Siddiqi, M. N. H., y Masum, M. A. (2013). 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Enfermedad de Newcastle. In Organizacion Mundial de Sanidad Animal (OIE) (Ed.), l Manual de las Pruebas de Diagnóstico y de las Vacunas para los Animales Terrestres (Octava). https://www.oie.int/es/sanidad-animal-en-el-mundo/enfermedades-de-los-animales/enfermedad-de-newcastle/#E Okechukwu, H., Chukwuedo, A., Eze, D., Igwe, A., Ihedioha, J., & Okoye, J. (2020). Triple La Sota re-vaccinations can protect laying chickens for 3 months against drop in egg production caused by velogenic viscerotropic Newcastle disease virus infection. Veterinary Medicine and Science, 6(3), 470-476. https://doi.org/10.1002/vms3.24 Olabode, A. O., Ndako, J. A., Echeonwu, G. O., Nwankiti, O. O., y Chukwuedo, A. A. (2010). Use of cracked maize as a carrier for NDV4vaccine in experimental vaccination of chickens. Virology Journal, 7, 1–5. https://doi.org/10.1186/1743-422X-7-67 Orsi, M. A., Zaroni, M. M. H., Doretto Júnior, L., Camillo, S. C. A., Ribeiro, S. A. M., Rosado Spilki, F., Buzinaro, M. da G., y Arns, C. W. (2009). Long-term stability studies on protection against Newcastle disease by commercial live vaccine used in Brazil. Biologicals, 37(4), 252–258. https://doi.org/10.1016/j.biologicals.2009.04.001 Owolodun, B. Y., y Ajiboye, E. A. (1975). Newcastle disease vaccines: a study of duration of immunity and properties of LaSota vaccine given in drinking water. British Veterinary Journal, 131(5), 580–585. https://doi.org/10.1016/S0007-1935(17)35191-6 Pandarangga, P., Brown, C. C., Miller, P. J., Haddas, R., Rehmani, S. F., Afonso, C. L., y Susta, L. (2016). Pathogenesis of New Strains of Newcastle Disease Virus From Israel and Pakistan. Veterinary Pathology, 53(4), 792–796. https://doi.org/10.1177/0300985815622972 Park, M., García-sastre, A., Cros, J. F., Basler, C. F., y Palese, P. (2003). 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Preventive Veterinary Medicine, 12,(1) 87-94. https://doi.org/10.1016/0167-5877(92)90071-M Tatár-kis, T., Fischer, A.J., Cazaban, C., Walkó-Kovács, E., Homonnay, Z.G., Velkers, F., Palya, V., Stegeman, .J.A. (2021). A Herpesvirus of Turkey-Based vector Vaccine ReducesTransmissionof Newcastle Disease Virus in Commercial Broiler Chickens with Maternally Derived Antibodies. Vaccines, 8, 614. https://doi.org/ 10.3390/vaccines8040614 Toyoda, T., Sakaguchi, T., Imai, K., Inocencio, N. M., Gotoh, B., Hamaguchi, M., y Nagai, Y. (1987). Structural comparison of the cleavage-activation site of the fusion glycoprotein between virulent and avirulent strains of newcastle disease virus. Virology, 158(1), 242–247. https://doi.org/10.1016/0042-6822(87)90261-3 Van Boven, M., Bouma, A., Fabri, T. H. F., Katsma, E., Hartog, L., y Koch, G. (2008). Herd immunity to Newcastle disease virus in poultry by vaccination. Avian Pathology, 37(1), 1–5. https://doi.org/10.1080/03079450701772391 Vrdoljak, A., Halas, M., y Süli, T. (2017). Vaccination of broilers against Newcastle disease in the presence of maternally derived antibodies. Tierarztliche Praxis Ausgabe G: Grosstiere - Nutztiere, 45(3), 151–158. https://doi.org/10.15653/TPG-160661 Wambura, P. N. (2009a). Oral vaccination of chickens against Newcastle disease with I-2 vaccine coated on oiled rice. Tropical Animal Health and Production, 41(2), 205–208. https://doi.org/10.1007/s11250-008-9176-8 Wambura, P. N. (2009b). Vaccination of chickens using raw rice coated with novel trehalose nano-organogels containing Newcastle disease (strain I-2) vaccine. Tropical Animal Health and Production, 41(5), 797–802. https://doi.org/10.1007/s11250-008-9254-y Wambura, P. N., y Wilson, C. (2009). Protective antibody response following oral vaccination of feral pigeons (Columba livia) with Newcastle disease vaccine (strain I-2) coated on oiled rice. 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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Álvarez Mira, Diana Marcelaa57bfb2c9b07cabe1edefc5e489c2cd4600Gómez Ramírez, Arlen Patricia1580f940e4cd8727b5e34b5b3e924b3e600Upegui Porras, Nicolás47502787ad3897f0119a36c1c1f133a52022-08-26T14:04:47Z2022-08-26T14:04:47Z2022-08https://repositorio.unal.edu.co/handle/unal/82134Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficas, tablasEl virus de la enfermedad de Newcastle (NDV) es uno de los problemas respiratorios más graves que causa importantes pérdidas económicas a los productores avícolas. La aplicación de vacunas vivas a través del alimento se ha postulado como un método eficaz para el control de la enfermedad, siendo ideal para reducir la presentación de signos clínicos y mortalidad en aves de traspatio; sin embargo, en Colombia el uso de esta estrategia es pobremente entendido. El presente estudio determinó la viabilidad de dos virus de baja virulencia del NDV (cepas VH y La Sota) mezclados con arroz y maíz bajo diferentes tipos de preparación y evaluó indicadores de respuesta inmune humoral e innata en aves luego de vacunarlas por vía oral. El título viral fue determinado por el método de Reed & Muench. Luego se realizó RT-qPCR para cuantificar la carga viral. Se obtuvieron resultados más consistentes mezclando la cepa VH con maíz cocido, por lo que la cepa LaSota y todos los otros vehículos fueron descartados del estudio. Posteriormente, 160 pollos machos (Babcock) fueron divididos aleatoriamente en cuatro grupos: un control negativo, y tres grupos vacunados (vía ocular, agua de bebida y vía maíz). Las aves fueron inmunizadas al día 28 de edad con la cepa VH por las vías señaladas y se tomaron sueros al día 1, 15 y 25 de edad y a los 7, 14 y 21 días post-vacunación para su evaluación por IH. Se tomaron parámetros productivos como peso y conversión acumulada. Se cuantificó la excreción viral y la expresión relativa de IL-6 e IFN-γ los días 2 y 4 post-vacunación en macerados de bazo por RT-qPCR. Finalmente, se calcularon índices de peso de órganos linfoides y se tomaron muestras de tejido digestivo, linfoide y respiratorio para evaluación histopatológica. Los resultados indicaron que la cepa VH mostró más estabilidad y el maíz cocido fue el candidato como vehículo. En cuanto a los títulos de anticuerpos hubo diferencias estadísticas significativas entre las vías de administración al día 7, pero no a los 14 y 21 días post-vacunación. No hubo diferencias en los índices linfoides, pero se observaron diferencias en el grado de lesión histopatológica en cornetes y laringe entre los grupos vacunados y el grupo control los días 2 y 4 post-vacunación. Se evidenció excreción viral en los grupos vacunados comparado con el control, siendo más alta en los grupos ocular y agua al día 2 post-vacunación y en el grupo maíz al día 4 post-vacunación. La expresión de IL-6 se evidenció temprano, pero la expresión de IFN-γ se evidenció solo hasta el día 4 post-vacunación. Este estudio mostró que la vía oral a través del alimento activa la respuesta inmune y que el maíz cocido y la cepa VH podrían ser una estrategia para un programa de control del NDV en aves de traspatio en Colombia. (Texto tomado de la fuente).Newcastle disease virus (NDV) is one of the most serious respiratory problems causing significant economic losses to poultry producers. The application of live vaccines through food has been postulated as an effective method to control the disease, being ideal to reduce the presence of clinical signs and mortality in backyard birds; however, in Colombia the use of this strategy is poorly understood. The present study determined the viability of two low pathogenic NDV viruses (VH and La Sota strains) mixed with rice and corn under different types of preparation and evaluated both of humoral and innate immune responses in birds after oral vaccination. The viral titer was determined by the Reed & Muench procedure. Then RT-qPCR was performed to quantify viral load. Consistently results were obtained mixing VH strain with cooked corn, so that LaSota strain and the other vehicles were discarded. Subsequently, 160 male chickens (Babcock) were randomly divided into four groups: a negative control, and three vaccinated groups (ocular route, drinking water and corn). The birds were immunized at day 28 of age with the VH strain by the indicated routes and sera were taken at days 1, 15 and 25 of age and at 7, 14 and 21-days postvaccination for evaluation by HI. Productive parameters such as weight and accumulated conversion were taken. Viral shedding and relative expression of IL-6 and IFN-γ were quantified on days 2 and 4 post-vaccination in spleen by RT-qPCR. Finally, lymphoid organ weight indices were calculated and digestive, lymphoid and respiratory tissue samples were taken for histopathological evaluation. The results indicated that the VH strain showed more stability and cooked corn was the candidate as a vehicle. Regarding antibody titers, there were statistically significant differences between administration routes on day 7, but there were no differences at 14 and 21 days post-vaccination. There were no differences in lymphoid indices, but differences in larynx and turbinates histopathological lesion scores were seen on days 2 and 4 post-vaccination. Cloacal viral shedding was evident in vaccinated groups compared to the control being higher in eye and water groups on day 2 and in corn group on day 4 post-vaccination. IL-6 expression was evidenced early, but IFNγ expression was evidenced only up to day 4 post-vaccination. This study showed that the oral route through food activates the immune response and that cooked corn and the VH strain could be a strategy to a NDV control program in backyard poultry in Colombia.Federación Nacional de AvicultoresFondo Nacional AvícolaIncluye anexosMaestríaMagíster en Salud Animal o Magíster en Producción AnimalMicrobiologia e Inmunologiaxix, 109 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina Veterinaria y de Zootecnia - Maestría en Salud y Producción AnimalDepartamento de Ciencias Para La Salud AnimalFacultad de Medicina Veterinaria y de ZootecniaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadas::636 - Producción animalNewcastle diseasePoultry - diseasesPoultry virus diseasesEnfermedad de NewcastleAves de corral - enfermedadesVirosis en las aves de corralRT-qPCRInhibición de la hemaglutinaciónMaízVirus de la enfermedad de NewcastleVacuna vivaAdministración oral en alimentoCornHemagglutination inhibitionNewcastle disease virusLive attenuated vaccineqRT-PCROral administration in foodViabilidad de cepas vacunales del virus de la enfermedad de Newcastle en arroz o maíz y respuesta inmune inducida en aves vacunadas vía alimentoViability of Newcastle disease virus vaccine strains in rice or corn and immune response induced in poultry vaccinated via feedTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbdi, R. 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