Estudio del efecto sobre el ligando de un complejo organoiridio en la regeneración de un cofactor enzimático
La catálisis híbrida es un nuevo concepto que surge como alternativa para el mejoramiento de las tecnologías de procesamiento de la biomasa, integrando la biocatálisis y la catálisis química en un sólo paso. Una de las aplicaciones que se ha venido desarrollando es la regeneración de los cofactores...
- Autores:
-
Sandoval Quiñonez, Karol Vanessa
- Tipo de recurso:
- http://purl.org/coar/version/c_b1a7d7d4d402bcce
- Fecha de publicación:
- 2016
- Institución:
- Universidad Industrial de Santander
- Repositorio:
- Repositorio UIS
- Idioma:
- spa
- OAI Identifier:
- oai:noesis.uis.edu.co:20.500.14071/34346
- Palabra clave:
- Catálisis Híbrida
Biomasa
Cofactor De Nicotinamida
Regeneración Del Cofactor
Complejo Organometálico.
Hybrid catalysis is a new concept which emerges as an alternative for improving biomass processing technologies by biocatalysis and chemical catalysis integration in a one pot process. Regeneration of nicotinamide cofactors (NADH/NAD+) is one of the applications that is being developed due to the large usage in biocatalytic processes and the high cost of using them in stoichiometric amounts in a large scale process. This has been carried out by organometallic complexes especially arene Ir (III)
Rh(III) and Ru(II) complexes which act as regioselective catalysts for regeneration and can couple enzymatic biosynthesis reactions
getting good results. This work analyzes the effect of the ligand of complex [(( 5 -Cp*)Ir(phen)(H2O)]2+ derivatives in the regeneration of NAD+ cofactor by means of 1H NMR spectroscopy. Eight complexes with different ligand substituents were synthetized for that purpose. Concentrations of NAD+ and hydride-complex were detected from 5 minutes of reaction therefore regeneration of NADH was proved. It is found that complexes with higher catalytic activity and fastness (TONmáx=1.92 y TOFmáx=12.92 h-1 ) have electron withdrawing groups
opposite case occurred with selectivity. Moreover
the behavior of concentration curves demonstrated a production of decomposition products then complexes were not completely selectives. Finally
complex with 5- chloro-1
10-phenanthroline ligand which has a weak electron withdrawing group showed the best characteristics among the synthetized complexes.
- Rights
- License
- Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)