Desarrollo de un biosensor amperométrico para la detección de peróxido de hidrogeno utilizando electrodos serigrafiados de oro modificados con peroxidasa de palma real (roystonea regia) y nanotubos de péptidos

Autores:

Vargas Ramirez, Jesus Alberto

Tipo de recurso:

http://purl.org/coar/version/c_b1a7d7d4d402bcce

Fecha de publicación:

2016

Institución:

Universidad Industrial de Santander

Repositorio:

Repositorio UIS

Idioma:

spa

Acceso en línea:: https://noesis.uis.edu.co/handle/20.500.14071/34941
https://noesis.uis.edu.co

Palabra clave:: Biosensor
Peroxidasa De Palma Real
Electrodo De Oro
Nanotubos De Péptidos.
In this work we developed a novel amperometric hydrogen peroxide biosensor based on the interaction of royal palm peroxidase (Roystonea regia) and peptide nanotubes by using glutaraldehyde as a linker on gold screen printed electrode. The development of the enzyme electrode consisted in four steps. The first step was the extraction and semi-purification of the peroxidase from royal palm tree. This process allowed to increase the specific activity and purification factor to 504.34 U / mg and 6.5 respectively. Then the electrode surface was modified with royal palm peroxidase by using peptide nanotube and glutaraldehyde
which allowed the electronic communication between the enzyme and the electrode surface. This step allowed the adsorption of peroxidase on the electrode surface. Bioelectrocatalytic reduction of hydrogen peroxide is observed at a redox potential -0.4 V vs Ag. This fact was demonstrated by the increasing on the electrocatalytic current of reduction at different concentrations of hydrogen peroxide. It was found that the electron transfer process was controlled superficially and depended on the pH of the solution. Based on the results from cronoamperometry
the enzyme electrode showed a linear response to hydrogen peroxide over a concentration range from 500 µM to 6000 µM with a detection limit of 432.71 µM and a sensibility of 0.024.

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