Estudio de la absorción óptica y espectros raman de un modelo electrolítico de medio extracelular

Se realizó el estudio de propiedades ópticas de una solución electrolítica que simula el medio extracelular sanguíneo (SME), empleando las técnicas de re_x001D_ectancia total atenuada FTIR ATR y dispersión Raman. Se comparó el espectro vibracional del agua con el de la solución electrolítica en la r...

Full description

Autores:
Moreno Gomez, Eduard Javier
Tipo de recurso:
http://purl.org/coar/version/c_b1a7d7d4d402bcce
Fecha de publicación:
2016
Institución:
Universidad Industrial de Santander
Repositorio:
Repositorio UIS
Idioma:
spa
OAI Identifier:
oai:noesis.uis.edu.co:20.500.14071/35362
Acceso en línea:
https://noesis.uis.edu.co/handle/20.500.14071/35362
https://noesis.uis.edu.co
Palabra clave:
Espectros
Dispersión
Absorción
Solución Electrolítica
Bandas Vibracionales.
The optic properties of an electrolytic solution were studied. This solution simulates the sanguineous extracellular medium (SME)
using both attenuated total re_x001D_ectance (FTIR ATR) and Raman scattering techniques. Water vibrational spectrum and electrolytic solution vibrational spectrum were compared in the 2700-4000 cm −1 region
where the OH-bond stretching bands are found. In order to prepare the electrolytic solution
three low concentrated salts were used: sodium chloride (N aCl)
potassium chloride (KCl) and calcium chloride (CaCl2). As a result of the low salt concentration employed
there was no found signi_x001C_cant spectrum changes. Because of this
it was necessary to study the e_x001B_ect of each salt
using aqueous solutions with higher concentrations than in the extracellular medium. Results show shifts on the intensity peaks as the concentration increase: from 3286 cm-1 to 3492 cm-1 with IR and from 3207 cm−1 to 3432 cm−1 in the case of Raman. The e_x001B_ect in FTIR ATR is more evident on the asymmetric stretching band
showing a peak in 3492 cm−1 . The sodium chloride solution showed a 58 cm−1 _x0015_shift at low frequencies; while potassium chloride and calcium chloride solutions exhibited lower shifts 44 cm−1 and 39 cm−1 respectively. The strongest e_x001B_ect
using Raman spectroscopy
was found in the symmetric stretching band
where the calcium chloride solution showed the largest shift toward 27 cm−1 frequencies
followed by the sodium chloride and potassium chloride solutions
which exhibited 21 cm−1 and 14 cm−1 shifts
respectively.
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License
Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)