Determining the proteomic profile changes in mabuya's sp placenta and ovary (squamata: scincidae)
Los reptiles son el grupo más diverso entre los vertebrados, proporcionan un sistema integral para estudios comparativos sobre metabolismo, fisiología animal y biología del desarrollo. Sin embargo, los datos moleculares disponibles son limitados y sólo recientemente han comenzado a llamar la atenció...
- Autores:
-
Hernandez Diaz, Yurany Nathaly
- Tipo de recurso:
- http://purl.org/coar/version/c_b1a7d7d4d402bcce
- Fecha de publicación:
- 2016
- Institución:
- Universidad Industrial de Santander
- Repositorio:
- Repositorio UIS
- Idioma:
- spa
- OAI Identifier:
- oai:noesis.uis.edu.co:20.500.14071/34261
- Palabra clave:
- Viviparidad
Gestación
Proteomica
Placentotrofía
Desarrollo Embrionario
Reptiles are the most diverse group among vertebrates
providing an integrated system for comparative studies on metabolic
animal physiology and developmental biology. However
the molecular data available is limited and only recently have started to call attention in the "omics" sciences. Mabuya sp is a viviparous placentrotrophic colombian skink with important reproductive features
including microlecithal eggs
early luteolysis
and the development of a highly specialized placenta implicated in respiratory exchange and the transference of all nutrients (ions
lipids and nitrogen
as an index of protein) necessary for embryo development. Our aim was to identify proteins that are differentially expressed in the ovary and placenta of Mabuya sp during early
mid and late gestation
their possible metabolic pathways and biological processes. We carried out a comparative proteomic analysis during gestation in both tissues by SDS-PAGE
2D-PAGE and MALDI-TOF. Differential -test P<0.05) ovarian and placental protein identification were related to housekeeping processes as cell structure
movement and energy. Proteins found in ovary are mainly associated with follicular development and its regulation. In the placenta
particularly during mid and late gestation when it is mature and highly specialized
protein expression is involved with functions like nutrient metabolism
transport
protein synthesis and embryonic development. This work provides preliminary insights about the proteins expressed and their physiological mechanisms in Mabuya during gestation.
- Rights
- License
- Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)