Electrodos serigrafiados de grafeno modificados con peroxidasa de pasto guinea (panicum maximun) para la detección enzimática de peróxido de hidrogeno
La construcción de nuevos dispositivos de detección es una de las tendencias más marcadas en cuanto a química analítica moderna se refiere. El objetivo de este trabajo de grado fue la modificación de electrodos serigrafiados de grafeno con peroxidasa de pasto guinea (Panicum maximum) para la detecci...
- Autores:
-
Solano Contreras, Xuxan Hasbleidy Del Rocio
- Tipo de recurso:
- http://purl.org/coar/version/c_b1a7d7d4d402bcce
- Fecha de publicación:
- 2016
- Institución:
- Universidad Industrial de Santander
- Repositorio:
- Repositorio UIS
- Idioma:
- spa
- OAI Identifier:
- oai:noesis.uis.edu.co:20.500.14071/34972
- Palabra clave:
- Pasto Guinea
Peroxidasa
Biosensor
Grafeno.
The main objective of this work was the modification of Graphene screen-printed electrodes with peroxidase of guinea grass (Panicum maximum) for the enzymatic detection of hydrogen peroxide. This work was development in three stages; first the enzyme was extracted and partially purified. The enzyme was obtained with a purification degree of 1
19
a specific activity of 724.7 U/mg with a yield of 24.3%. The second stage involved the immobilization of the enzyme over graphite screen-printed electrodes. And finally
the modified electrode was characterized through electrochemical techniques such as cyclic voltammetry
chronoamperometry and spectroscopy impedance. The bioelectrocatalytic reduction of hydrogen peroxidase was estimated to a redox potential of 1-10 V Ag/AgCl/KCl(sat). This process was diffusionally controlled over the surface of the electrode. Subsequently the biosensor response was studied under the influence of pH
obtaining a wide range of work since pH 5 until pH 10 and optimum work pH of 8.0. The displayed linear range was 100- results of chronoamperometry studies and calibration curves and This results were presented at the II Colombian Congress of Electrochemistry and 2nd Symposium on Nanoscience and Nanotechnology in poster mode.
- Rights
- License
- Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)