Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment

ABSTRACT: After cerebral ischemia or during several central nervous system (CNS) pathologies, exacerbated high glutamate concentration are released into the cerebral parenchyma producing a toxic environment that damages the components of the neurovascular unit (NVU) including the endothelial cells;...

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Autores:
Pineda López, Lina Gisela
Tipo de recurso:
Article of investigation
Fecha de publicación:
2020
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/17671
Acceso en línea:
http://hdl.handle.net/10495/17671
Palabra clave:
Glutamic acid
Amyloid precursor protein secretases
Endothelial cells
Astrocytes
http://id.nlm.nih.gov/mesh/D053829
http://id.nlm.nih.gov/mesh/D001253
http://id.nlm.nih.gov/mesh/D018698
http://id.nlm.nih.gov/mesh/D042783
Rights
embargoedAccess
License
Atribución-NoComercial-SinDerivadas 2.5 Colombia (CC BY-NC-ND 2.5 CO)
id UDEA2_cb987c7693efd0a4021cb8d84fa4b980
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/17671
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment
title Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment
spellingShingle Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment
Glutamic acid
Amyloid precursor protein secretases
Endothelial cells
Astrocytes
http://id.nlm.nih.gov/mesh/D053829
http://id.nlm.nih.gov/mesh/D001253
http://id.nlm.nih.gov/mesh/D018698
http://id.nlm.nih.gov/mesh/D042783
title_short Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment
title_full Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment
title_fullStr Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment
title_full_unstemmed Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment
title_sort Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environment
dc.creator.fl_str_mv Pineda López, Lina Gisela
dc.contributor.advisor.none.fl_str_mv Cardona Gómez, Gloria Patricia
Posada Duque, Rafael Andrés
dc.contributor.author.none.fl_str_mv Pineda López, Lina Gisela
dc.subject.mesh.none.fl_str_mv Glutamic acid
Amyloid precursor protein secretases
Endothelial cells
Astrocytes
topic Glutamic acid
Amyloid precursor protein secretases
Endothelial cells
Astrocytes
http://id.nlm.nih.gov/mesh/D053829
http://id.nlm.nih.gov/mesh/D001253
http://id.nlm.nih.gov/mesh/D018698
http://id.nlm.nih.gov/mesh/D042783
dc.subject.lcshuri.none.fl_str_mv http://id.nlm.nih.gov/mesh/D053829
dc.subject.meshuri.none.fl_str_mv http://id.nlm.nih.gov/mesh/D001253
http://id.nlm.nih.gov/mesh/D018698
http://id.nlm.nih.gov/mesh/D042783
description ABSTRACT: After cerebral ischemia or during several central nervous system (CNS) pathologies, exacerbated high glutamate concentration are released into the cerebral parenchyma producing a toxic environment that damages the components of the neurovascular unit (NVU) including the endothelial cells; which affects the function and structure of the blood-brain barrier. Astrocytes, glial cells that are closely related to the endothelium, change their gene expression profile in the face of toxicity and, depending of such profile, can be either protective or damage mediators. The enzyme BACE-1, involved in amyloidosis and vascular deterioration, increases its expression after an ischemic event even in astrocytes. Given the above, our aim was to determine the effect of BACE-1-inhibited astrocytes on endothelial cells integrity in a glutamate toxic environment by observing the effect of BACE-1 inhibition in endothelial cells and astrocytes cultures and endothelium-astrocyte co-cultures, through cytotoxicity and immunofluorescence assays. The results obtained suggest BACE1 inhibition protects endothelial cells integrity by reversing structural and inflammatory damage, also reverses astrocytic reactivity causing structural changes in cytoskeleton and cell inflammation. Furthermore, BACE1-inhibited astrocytes protect endothelial cells integrity regulating the Zonula occludens-1 distribution and decreasing inflammatory processes caused by glutamate toxicity. In summary, these results suggest inhibition of BACE1 under a glutamate toxicity has a protective effect on endothelium and astrocytes and their interactions.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-12-07T17:58:45Z
dc.date.available.none.fl_str_mv 2020-12-07T17:58:45Z
dc.date.issued.none.fl_str_mv 2020
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/17671
url http://hdl.handle.net/10495/17671
dc.language.iso.spa.fl_str_mv eng
language eng
dc.rights.*.fl_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia (CC BY-NC-ND 2.5 CO)
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rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia (CC BY-NC-ND 2.5 CO)
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.format.extent.spa.fl_str_mv 22
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.group.spa.fl_str_mv Grupo de Neurociencias de Antioquia
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
institution Universidad de Antioquia
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spelling Cardona Gómez, Gloria PatriciaPosada Duque, Rafael AndrésPineda López, Lina Gisela2020-12-07T17:58:45Z2020-12-07T17:58:45Z2020http://hdl.handle.net/10495/17671ABSTRACT: After cerebral ischemia or during several central nervous system (CNS) pathologies, exacerbated high glutamate concentration are released into the cerebral parenchyma producing a toxic environment that damages the components of the neurovascular unit (NVU) including the endothelial cells; which affects the function and structure of the blood-brain barrier. Astrocytes, glial cells that are closely related to the endothelium, change their gene expression profile in the face of toxicity and, depending of such profile, can be either protective or damage mediators. The enzyme BACE-1, involved in amyloidosis and vascular deterioration, increases its expression after an ischemic event even in astrocytes. Given the above, our aim was to determine the effect of BACE-1-inhibited astrocytes on endothelial cells integrity in a glutamate toxic environment by observing the effect of BACE-1 inhibition in endothelial cells and astrocytes cultures and endothelium-astrocyte co-cultures, through cytotoxicity and immunofluorescence assays. The results obtained suggest BACE1 inhibition protects endothelial cells integrity by reversing structural and inflammatory damage, also reverses astrocytic reactivity causing structural changes in cytoskeleton and cell inflammation. Furthermore, BACE1-inhibited astrocytes protect endothelial cells integrity regulating the Zonula occludens-1 distribution and decreasing inflammatory processes caused by glutamate toxicity. In summary, these results suggest inhibition of BACE1 under a glutamate toxicity has a protective effect on endothelium and astrocytes and their interactions.22application/pdfenginfo:eu-repo/semantics/draftinfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_b1a7d7d4d402bcceAtribución-NoComercial-SinDerivadas 2.5 Colombia (CC BY-NC-ND 2.5 CO)info:eu-repo/semantics/embargoedAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_f1cfhttps://creativecommons.org/licenses/by-nc-nd/4.0/Glutamic acidAmyloid precursor protein secretasesEndothelial cellsAstrocyteshttp://id.nlm.nih.gov/mesh/D053829http://id.nlm.nih.gov/mesh/D001253http://id.nlm.nih.gov/mesh/D018698http://id.nlm.nih.gov/mesh/D042783Effect of BACE1-inhibited astrocytes on endothelial cells in a glutamate toxic environmentGrupo de Neurociencias de AntioquiaMedellín, ColombiaBiólogaPregradoFacultad de Ciencias Exactas y Naturales. 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