Enzymatic hydrolysis of cassava stalks pretreated with the alkaline method
ABSTRACT: This study was developed with the aim of evaluating the pH, enzymatic complex load and temperature effects on the saccharification of pretreated cassava stalks (CS) using the response surface methodology (RSM). The factor levels evaluated were temperature 35-40°C, pH 4.0-5.0 and dose of en...
- Autores:
-
Castaño P, Hader
Reales A, Juan G.
Zapata Montoya, José Edgar
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2015
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/24032
- Acceso en línea:
- http://hdl.handle.net/10495/24032
- Palabra clave:
- Digestión
Digestion
Almidón
Starch
Yuca
Cassava
Enzimólisis
Enzymolysis
Lignocelulosa
Lignocellulose
Manihot esculenta Crantz
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
| Summary: | ABSTRACT: This study was developed with the aim of evaluating the pH, enzymatic complex load and temperature effects on the saccharification of pretreated cassava stalks (CS) using the response surface methodology (RSM). The factor levels evaluated were temperature 35-40°C, pH 4.0-5.0 and dose of enzymatic complex Accellerase 1500™ 2.9 - 14.5 FPU/g of substrate. The reducing sugar (RS) response was used. The pH was controlled through the use of hydrochloric acid and sodium hydroxide solutions and the system was shaken orbitally at 120 rpm with a solids loading of 10% w/v. The fitted model showed that the optimal operating conditions were: pH 4.0, 38°C and enzyme dose of 14.5 FPU/g substrate, reaching a sugar concentration of 18.4 g L-1. |
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