Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
ABSTRACT:An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation cont...
- Autores:
-
Montoya Cuervo, Edwin Andrés
Daza Figueredo, Juan Alejandro
Muñoz Herrera, Diana Lorena
Ríos Ramírez, Yesmit Karina
Taylor Orozco, Viviana Milena
Cedeño, David
Vélez Bernal, Iván Darío
Echeverri López, Luis Fernando
Robledo Restrepo, Sara María
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2015
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/32966
- Acceso en línea:
- https://hdl.handle.net/10495/32966
- Palabra clave:
- Antracenos
Anthracenes
Línea Celular
Cell Line
Cricetinae
Leishmania
Leishmaniasis Cutánea
Leishmaniasis, Cutaneous
Perileno
Perylene
Fotoquimioterapia
Photochemotherapy
Fármacos Fotosensibilizantes
Photosensitizing Agents
Células U937
U937 Cells
Cicatrización de Heridas
Wound Healing
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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Repositorio UdeA |
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|
dc.title.spa.fl_str_mv |
Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies |
title |
Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies |
spellingShingle |
Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies Antracenos Anthracenes Línea Celular Cell Line Cricetinae Leishmania Leishmaniasis Cutánea Leishmaniasis, Cutaneous Perileno Perylene Fotoquimioterapia Photochemotherapy Fármacos Fotosensibilizantes Photosensitizing Agents Células U937 U937 Cells Cicatrización de Heridas Wound Healing |
title_short |
Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies |
title_full |
Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies |
title_fullStr |
Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies |
title_full_unstemmed |
Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies |
title_sort |
Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies |
dc.creator.fl_str_mv |
Montoya Cuervo, Edwin Andrés Daza Figueredo, Juan Alejandro Muñoz Herrera, Diana Lorena Ríos Ramírez, Yesmit Karina Taylor Orozco, Viviana Milena Cedeño, David Vélez Bernal, Iván Darío Echeverri López, Luis Fernando Robledo Restrepo, Sara María |
dc.contributor.author.none.fl_str_mv |
Montoya Cuervo, Edwin Andrés Daza Figueredo, Juan Alejandro Muñoz Herrera, Diana Lorena Ríos Ramírez, Yesmit Karina Taylor Orozco, Viviana Milena Cedeño, David Vélez Bernal, Iván Darío Echeverri López, Luis Fernando Robledo Restrepo, Sara María |
dc.subject.decs.none.fl_str_mv |
Antracenos Anthracenes Línea Celular Cell Line Cricetinae Leishmania Leishmaniasis Cutánea Leishmaniasis, Cutaneous Perileno Perylene Fotoquimioterapia Photochemotherapy Fármacos Fotosensibilizantes Photosensitizing Agents Células U937 U937 Cells Cicatrización de Heridas |
topic |
Antracenos Anthracenes Línea Celular Cell Line Cricetinae Leishmania Leishmaniasis Cutánea Leishmaniasis, Cutaneous Perileno Perylene Fotoquimioterapia Photochemotherapy Fármacos Fotosensibilizantes Photosensitizing Agents Células U937 U937 Cells Cicatrización de Heridas Wound Healing |
dc.subject.lemb.none.fl_str_mv |
Wound Healing |
description |
ABSTRACT:An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation containing 0.5% hypericin was developed and assayed in vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effect in vitro against L. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing of L. pana- mensis-induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm2, 15 min), twice a week for 3 weeks. |
publishDate |
2015 |
dc.date.issued.none.fl_str_mv |
2015 |
dc.date.accessioned.none.fl_str_mv |
2022-12-25T16:11:43Z |
dc.date.available.none.fl_str_mv |
2022-12-25T16:11:43Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D, Vélez ID, Echeverri F, Robledo SM. Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies. Antimicrob Agents Chemother. 2015 Sep;59(9):5804-13. doi: 10.1128/AAC.00545-15. |
dc.identifier.issn.none.fl_str_mv |
0066-4804 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/32966 |
dc.identifier.doi.none.fl_str_mv |
10.1128/AAC.00545-15 |
dc.identifier.eissn.none.fl_str_mv |
1098-6596 |
identifier_str_mv |
Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D, Vélez ID, Echeverri F, Robledo SM. Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies. Antimicrob Agents Chemother. 2015 Sep;59(9):5804-13. doi: 10.1128/AAC.00545-15. 0066-4804 10.1128/AAC.00545-15 1098-6596 |
url |
https://hdl.handle.net/10495/32966 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Antimicrob. Agents. Chemother. |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.extent.spa.fl_str_mv |
10 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
American Society for Microbiology |
dc.publisher.group.spa.fl_str_mv |
Programa de Estudio y Control de Enfermedades Tropicales (PECET) Química Orgánica de Productos Naturales |
dc.publisher.place.spa.fl_str_mv |
Washington, Estados Unidos |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/1/MontoyaAndres_2015_DevelopmentNovelFormulation.pdf https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/2/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/3/license.txt |
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bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173254081642496 |
spelling |
Montoya Cuervo, Edwin AndrésDaza Figueredo, Juan AlejandroMuñoz Herrera, Diana LorenaRíos Ramírez, Yesmit KarinaTaylor Orozco, Viviana MilenaCedeño, DavidVélez Bernal, Iván DaríoEcheverri López, Luis FernandoRobledo Restrepo, Sara María2022-12-25T16:11:43Z2022-12-25T16:11:43Z2015Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D, Vélez ID, Echeverri F, Robledo SM. Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies. Antimicrob Agents Chemother. 2015 Sep;59(9):5804-13. doi: 10.1128/AAC.00545-15.0066-4804https://hdl.handle.net/10495/3296610.1128/AAC.00545-151098-6596ABSTRACT:An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation containing 0.5% hypericin was developed and assayed in vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effect in vitro against L. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing of L. pana- mensis-induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm2, 15 min), twice a week for 3 weeks.COL0015339COL001509910application/pdfengAmerican Society for MicrobiologyPrograma de Estudio y Control de Enfermedades Tropicales (PECET)Química Orgánica de Productos NaturalesWashington, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studiesAntracenosAnthracenesLínea CelularCell LineCricetinaeLeishmaniaLeishmaniasis CutáneaLeishmaniasis, CutaneousPerilenoPeryleneFotoquimioterapiaPhotochemotherapyFármacos FotosensibilizantesPhotosensitizing AgentsCélulas U937U937 CellsCicatrización de HeridasWound HealingAntimicrob. Agents. Chemother.Antimicrobial Agents and Chemotherapy58045813599ORIGINALMontoyaAndres_2015_DevelopmentNovelFormulation.pdfMontoyaAndres_2015_DevelopmentNovelFormulation.pdfArtículo de investigaciónapplication/pdf844482https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/1/MontoyaAndres_2015_DevelopmentNovelFormulation.pdffbc2a2d22bb3564d1cd000a244ea08bcMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/32966oai:bibliotecadigital.udea.edu.co:10495/329662022-12-25 11:11:44.214Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |