Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies

ABSTRACT:An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation cont...

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Autores:
Montoya Cuervo, Edwin Andrés
Daza Figueredo, Juan Alejandro
Muñoz Herrera, Diana Lorena
Ríos Ramírez, Yesmit Karina
Taylor Orozco, Viviana Milena
Cedeño, David
Vélez Bernal, Iván Darío
Echeverri López, Luis Fernando
Robledo Restrepo, Sara María
Tipo de recurso:
Article of investigation
Fecha de publicación:
2015
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/32966
Acceso en línea:
https://hdl.handle.net/10495/32966
Palabra clave:
Antracenos
Anthracenes
Línea Celular
Cell Line
Cricetinae
Leishmania
Leishmaniasis Cutánea
Leishmaniasis, Cutaneous
Perileno
Perylene
Fotoquimioterapia
Photochemotherapy
Fármacos Fotosensibilizantes
Photosensitizing Agents
Células U937
U937 Cells
Cicatrización de Heridas
Wound Healing
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
id UDEA2_9af85a8cdf68cc7cccd538d7c5e1e695
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/32966
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
title Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
spellingShingle Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
Antracenos
Anthracenes
Línea Celular
Cell Line
Cricetinae
Leishmania
Leishmaniasis Cutánea
Leishmaniasis, Cutaneous
Perileno
Perylene
Fotoquimioterapia
Photochemotherapy
Fármacos Fotosensibilizantes
Photosensitizing Agents
Células U937
U937 Cells
Cicatrización de Heridas
Wound Healing
title_short Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
title_full Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
title_fullStr Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
title_full_unstemmed Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
title_sort Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies
dc.creator.fl_str_mv Montoya Cuervo, Edwin Andrés
Daza Figueredo, Juan Alejandro
Muñoz Herrera, Diana Lorena
Ríos Ramírez, Yesmit Karina
Taylor Orozco, Viviana Milena
Cedeño, David
Vélez Bernal, Iván Darío
Echeverri López, Luis Fernando
Robledo Restrepo, Sara María
dc.contributor.author.none.fl_str_mv Montoya Cuervo, Edwin Andrés
Daza Figueredo, Juan Alejandro
Muñoz Herrera, Diana Lorena
Ríos Ramírez, Yesmit Karina
Taylor Orozco, Viviana Milena
Cedeño, David
Vélez Bernal, Iván Darío
Echeverri López, Luis Fernando
Robledo Restrepo, Sara María
dc.subject.decs.none.fl_str_mv Antracenos
Anthracenes
Línea Celular
Cell Line
Cricetinae
Leishmania
Leishmaniasis Cutánea
Leishmaniasis, Cutaneous
Perileno
Perylene
Fotoquimioterapia
Photochemotherapy
Fármacos Fotosensibilizantes
Photosensitizing Agents
Células U937
U937 Cells
Cicatrización de Heridas
topic Antracenos
Anthracenes
Línea Celular
Cell Line
Cricetinae
Leishmania
Leishmaniasis Cutánea
Leishmaniasis, Cutaneous
Perileno
Perylene
Fotoquimioterapia
Photochemotherapy
Fármacos Fotosensibilizantes
Photosensitizing Agents
Células U937
U937 Cells
Cicatrización de Heridas
Wound Healing
dc.subject.lemb.none.fl_str_mv Wound Healing
description ABSTRACT:An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation containing 0.5% hypericin was developed and assayed in vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effect in vitro against L. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing of L. pana- mensis-induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm2, 15 min), twice a week for 3 weeks.
publishDate 2015
dc.date.issued.none.fl_str_mv 2015
dc.date.accessioned.none.fl_str_mv 2022-12-25T16:11:43Z
dc.date.available.none.fl_str_mv 2022-12-25T16:11:43Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D, Vélez ID, Echeverri F, Robledo SM. Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies. Antimicrob Agents Chemother. 2015 Sep;59(9):5804-13. doi: 10.1128/AAC.00545-15.
dc.identifier.issn.none.fl_str_mv 0066-4804
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/32966
dc.identifier.doi.none.fl_str_mv 10.1128/AAC.00545-15
dc.identifier.eissn.none.fl_str_mv 1098-6596
identifier_str_mv Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D, Vélez ID, Echeverri F, Robledo SM. Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies. Antimicrob Agents Chemother. 2015 Sep;59(9):5804-13. doi: 10.1128/AAC.00545-15.
0066-4804
10.1128/AAC.00545-15
1098-6596
url https://hdl.handle.net/10495/32966
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Antimicrob. Agents. Chemother.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.spa.fl_str_mv American Society for Microbiology
dc.publisher.group.spa.fl_str_mv Programa de Estudio y Control de Enfermedades Tropicales (PECET)
Química Orgánica de Productos Naturales
dc.publisher.place.spa.fl_str_mv Washington, Estados Unidos
institution Universidad de Antioquia
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spelling Montoya Cuervo, Edwin AndrésDaza Figueredo, Juan AlejandroMuñoz Herrera, Diana LorenaRíos Ramírez, Yesmit KarinaTaylor Orozco, Viviana MilenaCedeño, DavidVélez Bernal, Iván DaríoEcheverri López, Luis FernandoRobledo Restrepo, Sara María2022-12-25T16:11:43Z2022-12-25T16:11:43Z2015Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D, Vélez ID, Echeverri F, Robledo SM. Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies. Antimicrob Agents Chemother. 2015 Sep;59(9):5804-13. doi: 10.1128/AAC.00545-15.0066-4804https://hdl.handle.net/10495/3296610.1128/AAC.00545-151098-6596ABSTRACT:An evaluation of the leishmanicidal activity in vitro and in vivo of hypericin, an expanded-spectrum photosensitizer found in Hypericum perforatum, is presented. Hypericin was evaluated against intracellular amastigotes in vitro of Leishmania (Viannia) panamensis. A topical formulation containing 0.5% hypericin was developed and assayed in vivo in a hamster model of cutaneous leishmaniasis. Results demonstrate that hypericin induces a significant antiamastigote effect in vitro against L. panamensis by decreasing the number of parasites inside infected cells. The topical formulation of 0.5% hypericin allows healing of L. pana- mensis-induced lesions upon a topical application of 40 mg/day plus visible-light irradiation (5 J/cm2, 15 min), twice a week for 3 weeks.COL0015339COL001509910application/pdfengAmerican Society for MicrobiologyPrograma de Estudio y Control de Enfermedades Tropicales (PECET)Química Orgánica de Productos NaturalesWashington, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studiesAntracenosAnthracenesLínea CelularCell LineCricetinaeLeishmaniaLeishmaniasis CutáneaLeishmaniasis, CutaneousPerilenoPeryleneFotoquimioterapiaPhotochemotherapyFármacos FotosensibilizantesPhotosensitizing AgentsCélulas U937U937 CellsCicatrización de HeridasWound HealingAntimicrob. Agents. Chemother.Antimicrobial Agents and Chemotherapy58045813599ORIGINALMontoyaAndres_2015_DevelopmentNovelFormulation.pdfMontoyaAndres_2015_DevelopmentNovelFormulation.pdfArtículo de investigaciónapplication/pdf844482https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/1/MontoyaAndres_2015_DevelopmentNovelFormulation.pdffbc2a2d22bb3564d1cd000a244ea08bcMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/32966/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/32966oai:bibliotecadigital.udea.edu.co:10495/329662022-12-25 11:11:44.214Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=