Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1

ABSTRACT: Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, capturing viral particles, facilitating the infection of CD4+ T-cells. Vitamin D (VitD) has shown to decrease T cell activation, reducing susceptibility to HIV-1 infection of CD4+ T-cells in vitro; however, if Vit...

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Autores:
Álvarez Mesa, Natalia
González Díaz, Sandra Milena
Hernández López, Juan Carlos
Rugeles López, María Teresa
Aguilar Jiménez, Wbeimar
Tipo de recurso:
Article of investigation
Fecha de publicación:
2022
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/29865
Acceso en línea:
https://hdl.handle.net/10495/29865
Palabra clave:
Linfocitos T CD4-Positivos
CD4-Positive T-Lymphocytes
Calcitriol - farmacología
Calcitriol - pharmacology
Moléculas de Adhesión Celular
Cell Adhesion Molecules
Infecciones por VIH
HIV Infections
Células Dendríticas
Dendritic Cells
Lectina 1 Similar a Ig de Unión al Ácido Siálico
Sialic Acid Binding Ig-like Lectin 1
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
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oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/29865
network_acronym_str UDEA2
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repository_id_str
dc.title.spa.fl_str_mv Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
spellingShingle Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
Linfocitos T CD4-Positivos
CD4-Positive T-Lymphocytes
Calcitriol - farmacología
Calcitriol - pharmacology
Moléculas de Adhesión Celular
Cell Adhesion Molecules
Infecciones por VIH
HIV Infections
Células Dendríticas
Dendritic Cells
Lectina 1 Similar a Ig de Unión al Ácido Siálico
Sialic Acid Binding Ig-like Lectin 1
title_short Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title_full Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title_fullStr Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title_full_unstemmed Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
title_sort Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1
dc.creator.fl_str_mv Álvarez Mesa, Natalia
González Díaz, Sandra Milena
Hernández López, Juan Carlos
Rugeles López, María Teresa
Aguilar Jiménez, Wbeimar
dc.contributor.author.none.fl_str_mv Álvarez Mesa, Natalia
González Díaz, Sandra Milena
Hernández López, Juan Carlos
Rugeles López, María Teresa
Aguilar Jiménez, Wbeimar
dc.subject.decs.none.fl_str_mv Linfocitos T CD4-Positivos
CD4-Positive T-Lymphocytes
Calcitriol - farmacología
Calcitriol - pharmacology
Moléculas de Adhesión Celular
Cell Adhesion Molecules
Infecciones por VIH
HIV Infections
Células Dendríticas
Dendritic Cells
Lectina 1 Similar a Ig de Unión al Ácido Siálico
Sialic Acid Binding Ig-like Lectin 1
topic Linfocitos T CD4-Positivos
CD4-Positive T-Lymphocytes
Calcitriol - farmacología
Calcitriol - pharmacology
Moléculas de Adhesión Celular
Cell Adhesion Molecules
Infecciones por VIH
HIV Infections
Células Dendríticas
Dendritic Cells
Lectina 1 Similar a Ig de Unión al Ácido Siálico
Sialic Acid Binding Ig-like Lectin 1
description ABSTRACT: Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, capturing viral particles, facilitating the infection of CD4+ T-cells. Vitamin D (VitD) has shown to decrease T cell activation, reducing susceptibility to HIV-1 infection of CD4+ T-cells in vitro; however, if VitD decreases viral transfer from DCs to CD4+ T-cells is unknown. In this study, we co-cultured HIV-1-pulsed immature and LPS mature monocytes-derived DCs (iDCs and LmDCs, respectively), differentiated in presence or absence of calcitriol (VitD active form), with PHA-activated autologous CD4+ T-cells from 16 healthy donors. In co-cultures of iDCs and LmDCs treated with calcitriol, there was a significant decrease in frequency of infected CD4+ T-cells, evaluated by flow cytometry. However, p24 levels evaluated by ELISA were not significantly reduced in culture supernatants. Moreover, calcitriol-treated iDCs exhibited decreased expression of genes involved in HIV-1 transfer compared to the control. Both, calcitriol-treated iDCs and LmDCs exhibit a similar gene expression profile, probably related to a transcriptional balance achieved after long treatment with calcitriol. Since calcitriol-differentiated DCs express on their surface a lower amount of DC-SIGN and SIGLEC-1 molecules, widely associated with HIV-1 transfer, suggesting that this mechanism contributes to a lower transfer of viral particles by the DCs.
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-07-25T21:09:44Z
dc.date.available.none.fl_str_mv 2022-07-25T21:09:44Z
dc.date.issued.none.fl_str_mv 2022
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
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dc.identifier.citation.spa.fl_str_mv Alvarez N, Gonzalez SM, Hernandez JC, Rugeles MT, Aguilar-Jimenez W. Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1. PLoS One. 2022 Jul 8;17(7):e0269932. doi: 10.1371/journal.pone.0269932.
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/29865
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0269932
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv Alvarez N, Gonzalez SM, Hernandez JC, Rugeles MT, Aguilar-Jimenez W. Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1. PLoS One. 2022 Jul 8;17(7):e0269932. doi: 10.1371/journal.pone.0269932.
10.1371/journal.pone.0269932
1932-6203
url https://hdl.handle.net/10495/29865
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv PLoS ONE.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.extent.spa.fl_str_mv 14
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Public Library of Science
dc.publisher.group.spa.fl_str_mv Inmunovirología
dc.publisher.place.spa.fl_str_mv San Francisco, Estados Unidos
institution Universidad de Antioquia
bitstream.url.fl_str_mv https://bibliotecadigital.udea.edu.co/bitstream/10495/29865/1/AlvarezNatalia_2022_CalcitriolDecreasesHIV1.pdf
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spelling Álvarez Mesa, NataliaGonzález Díaz, Sandra MilenaHernández López, Juan CarlosRugeles López, María TeresaAguilar Jiménez, Wbeimar2022-07-25T21:09:44Z2022-07-25T21:09:44Z2022Alvarez N, Gonzalez SM, Hernandez JC, Rugeles MT, Aguilar-Jimenez W. Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1. PLoS One. 2022 Jul 8;17(7):e0269932. doi: 10.1371/journal.pone.0269932.https://hdl.handle.net/10495/2986510.1371/journal.pone.02699321932-6203ABSTRACT: Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, capturing viral particles, facilitating the infection of CD4+ T-cells. Vitamin D (VitD) has shown to decrease T cell activation, reducing susceptibility to HIV-1 infection of CD4+ T-cells in vitro; however, if VitD decreases viral transfer from DCs to CD4+ T-cells is unknown. In this study, we co-cultured HIV-1-pulsed immature and LPS mature monocytes-derived DCs (iDCs and LmDCs, respectively), differentiated in presence or absence of calcitriol (VitD active form), with PHA-activated autologous CD4+ T-cells from 16 healthy donors. In co-cultures of iDCs and LmDCs treated with calcitriol, there was a significant decrease in frequency of infected CD4+ T-cells, evaluated by flow cytometry. However, p24 levels evaluated by ELISA were not significantly reduced in culture supernatants. Moreover, calcitriol-treated iDCs exhibited decreased expression of genes involved in HIV-1 transfer compared to the control. Both, calcitriol-treated iDCs and LmDCs exhibit a similar gene expression profile, probably related to a transcriptional balance achieved after long treatment with calcitriol. Since calcitriol-differentiated DCs express on their surface a lower amount of DC-SIGN and SIGLEC-1 molecules, widely associated with HIV-1 transfer, suggesting that this mechanism contributes to a lower transfer of viral particles by the DCs.COL001244414application/pdfengPublic Library of ScienceInmunovirologíaSan Francisco, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1Linfocitos T CD4-PositivosCD4-Positive T-LymphocytesCalcitriol - farmacologíaCalcitriol - pharmacologyMoléculas de Adhesión CelularCell Adhesion MoleculesInfecciones por VIHHIV InfectionsCélulas DendríticasDendritic CellsLectina 1 Similar a Ig de Unión al Ácido SiálicoSialic Acid Binding Ig-like Lectin 1PLoS ONE.PLoS ONE114177ORIGINALAlvarezNatalia_2022_CalcitriolDecreasesHIV1.pdfAlvarezNatalia_2022_CalcitriolDecreasesHIV1.pdfArtículo de investigaciónapplication/pdf1432180https://bibliotecadigital.udea.edu.co/bitstream/10495/29865/1/AlvarezNatalia_2022_CalcitriolDecreasesHIV1.pdf8b6e0c2b836c99e456833780ef37167dMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/29865/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/29865/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/29865oai:bibliotecadigital.udea.edu.co:10495/298652022-07-25 16:09:45.022Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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