Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1

ABSTRACT: Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, capturing viral particles, facilitating the infection of CD4+ T-cells. Vitamin D (VitD) has shown to decrease T cell activation, reducing susceptibility to HIV-1 infection of CD4+ T-cells in vitro; however, if VitD decreases viral transfer from DCs to CD4+ T-cells is unknown. In this study, we co-cultured HIV-1-pulsed immature and LPS mature monocytes-derived DCs (iDCs and LmDCs, respectively), differentiated in presence or absence of calcitriol (VitD active form), with PHA-activated autologous CD4+ T-cells from 16 healthy donors. In co-cultures of iDCs and LmDCs treated with calcitriol, there was a significant decrease in frequency of infected CD4+ T-cells, evaluated by flow cytometry. However, p24 levels evaluated by ELISA were not significantly reduced in culture supernatants. Moreover, calcitriol-treated iDCs exhibited decreased expression of genes involved in HIV-1 transfer compared to the control. Both, calcitriol-treated iDCs and LmDCs exhibit a similar gene expression profile, probably related to a transcriptional balance achieved after long treatment with calcitriol. Since calcitriol-differentiated DCs express on their surface a lower amount of DC-SIGN and SIGLEC-1 molecules, widely associated with HIV-1 transfer, suggesting that this mechanism contributes to a lower transfer of viral particles by the DCs.

• Autores: Álvarez Mesa, Natalia; González Díaz, Sandra Milena; Hernández López, Juan Carlos; Rugeles López, María Teresa; Aguilar Jiménez, Wbeimar
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2022
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/29865
• Acceso en línea: https://hdl.handle.net/10495/29865
• Palabra clave: Linfocitos T CD4-Positivos; CD4-Positive T-Lymphocytes; Calcitriol - farmacología; Calcitriol - pharmacology; Moléculas de Adhesión Celular; Cell Adhesion Molecules; Infecciones por VIH; HIV Infections; Células Dendríticas; Dendritic Cells; Lectina 1 Similar a Ig de Unión al Ácido Siálico; Sialic Acid Binding Ig-like Lectin 1
• Rights: openAccess
• License: http://creativecommons.org/licenses/by/2.5/co/