Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria
La conservación de las proteínas de origen animal , representa un reto para la industria alimentaria, ya que, además de asegurar la inocuidad del producto, de igual forma debe reducir al máximo las pérdidas económicas que puedan presentarse en consecuencia. 1 La presente monografía pretende fundamen...
- Autores:
-
Diaz Cañón, Yenny Alejandra
Salgado Gasca, Jeimi Tatiana
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Colegio Mayor de Cundinamarca
- Repositorio:
- Repositorio Colegio Mayor de Cundinamarca
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unicolmayor.edu.co:unicolmayor/5670
- Acceso en línea:
- https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5670
- Palabra clave:
- Microbiología alimentaria
Bioconservantes
Bacterias ácido lácticas
Bacteriocinas
Carne de pollo
- Rights
- closedAccess
- License
- Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022
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dc.title.spa.fl_str_mv |
Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria |
title |
Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria |
spellingShingle |
Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria Microbiología alimentaria Bioconservantes Bacterias ácido lácticas Bacteriocinas Carne de pollo |
title_short |
Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria |
title_full |
Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria |
title_fullStr |
Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria |
title_full_unstemmed |
Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria |
title_sort |
Bacterias ácido lácticas como bioconservantes en carnes en la industria alimentaria |
dc.creator.fl_str_mv |
Diaz Cañón, Yenny Alejandra Salgado Gasca, Jeimi Tatiana |
dc.contributor.advisor.none.fl_str_mv |
Lancheros Díaz, Ana Graciela |
dc.contributor.author.none.fl_str_mv |
Diaz Cañón, Yenny Alejandra Salgado Gasca, Jeimi Tatiana |
dc.subject.lemb.none.fl_str_mv |
Microbiología alimentaria Bioconservantes |
topic |
Microbiología alimentaria Bioconservantes Bacterias ácido lácticas Bacteriocinas Carne de pollo |
dc.subject.proposal.spa.fl_str_mv |
Bacterias ácido lácticas Bacteriocinas Carne de pollo |
description |
La conservación de las proteínas de origen animal , representa un reto para la industria alimentaria, ya que, además de asegurar la inocuidad del producto, de igual forma debe reducir al máximo las pérdidas económicas que puedan presentarse en consecuencia. 1 La presente monografía pretende fundamentar la importancia de nuevas alternativas para la conservación de carnes, haciendo uso de bacterias ácido lácticas (BAL), microorganismos habitualmente utilizados cómo probióticos en diferentes alimentos, gracias a que poseen mecanismos prometedores en funciones de biopreservación. Para ello, la presente revisión bibliográfica realizada en bases datos de carácter científicoacadémico tales cómo Scopus, ScienceDirect, Nature, ELSEVIER, Multidisciplinary Digital Publishing Institute (MDPI), Pubmed, Taylor and Francis; destaca diferentes mecanismos, tales cómo la producción de ácido láctico, ácido acético, peróxido de Hidrógeno, dióxido de Carbono, así mismo la producción de metabolitos propios, dentro de ellos las bacteriocinas que utilizadas de forma individual o en conjunto tienen una alta versatilidad en la industria, agregando el hecho de que dada la naturaleza de estos compuestos, permite describirlos como sustancias seguras para la salud humana, por lo tanto, se deduce el uso de las BAL como potentes agentes microbianos además de ser una alternativa alimentaria segura y rentable. |
publishDate |
2022 |
dc.date.accessioned.none.fl_str_mv |
2022-09-15T16:16:14Z |
dc.date.available.none.fl_str_mv |
2022-09-15T16:16:14Z |
dc.date.issued.none.fl_str_mv |
2022 |
dc.type.spa.fl_str_mv |
Trabajo de grado - Pregrado |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_7a1f |
dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.content.spa.fl_str_mv |
Text |
dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/TP |
dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
http://purl.org/coar/resource_type/c_7a1f |
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publishedVersion |
dc.identifier.uri.none.fl_str_mv |
https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5670 |
url |
https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5670 |
dc.language.iso.spa.fl_str_mv |
spa |
language |
spa |
dc.relation.references.spa.fl_str_mv |
Thyagarajan D, Punniamurthy N, Bhaisare DB, Thyagarajan D, Churchil RR, Punniamurthy N. Bacterial Pathogens in Chicken Meat: Review. Print International Journal of Life Sciences Research. [Internet]. 2014. [Cited 20 nov 2020]; 2(3): 1-7. Available in: https://www.researchgate.net/profile/RChurchil/publication/347510610_Bacterial_Pathogens_in_Chicken_Meat_Review /links/5fdf431f45851553a0d65825/Bacterial-Pathogens-in-Chicken-MeatReview.pdf Información estadística - FENAVI - Federación Nacional de Avicultores de Colombia. [Cited 20 nov 2020]. Available in: https://fenavi.org/informacionestadistica/ Informacion estadística-FEDEGAN-Federación Colombiana de Ganaderos. [Cited 20 nov 2020]. Available in: https://www.fedegan.org.co/estadisticas/consumo-0 . Bintsis T. Lactic acid bacteria: their applications in foods . J Bacteriol Mycol Open Access. [Internet]. 2018. [Cited 14 oct 2020];6(2):89–94. Available in: 10.15406/jbmoa.2018.06.00182 Gálves A, López R, Abriouel H, Valdivia A, Ben N. Application of Bacteriocins in the Control of Foodborne Pathogenic and Spoilage Bacteria. Revista Taylor & Francis. [Internet]. 2008. [Cited 14 oct 2020]; 28(2). Available in : https://www.tandfonline.com/doi/full/10.1080/07388550802107202 Saddam A, Salam I. Screening of Antibacterial Activity of Lactic Acid Bacteria Against Different Pathogens Found in Vacuum-Packaged Meat Products. Revista Foodborne Pathogens and disease. [Internet].2009. [Cited 15 oct 2020]; 6(9). Available in: https://www.liebertpub.com/doi/10.1089/fpd.2009.0272?url_ver=Z39.88- 2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmed Norrung B, Andersen J, Buncic S. Main Concerns of Pathogenic Microorganisms in Meat. Recopilado del libro: Safety of meat and processed. España: Springer; 2009. [Internet]. [Cited 20 nov 2020]. Available in: https://link.springer.com/chapter/10.1007/978-0-387-89026-5_1 Nychas J, Dourou D, Skandamis P, Koutsoumanis K, Baranyi J, Sofos J. Effect of microbial cell‐ free meat extract on the growth of spoilage bacteria. Journal of Applied Microbiology. [Internet]. 2009. [Cited 14 oct 2020]; 1819-1829. Available in: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365- 2672.2009.04377.x Ercolini D, Russo F, Nasi A, Ferranti P, Villani F. Mesophilic and Psychrotrophic Bacteria from Meat and Their Spoilage Potential in vitro and in beef. Potential in Vitro and in Beef. American Society for Microbiology. [Internet]. 2009. [Cited 15 oct 2020]; 75(7). Available in: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663181/ Fadda S, López C, Vignolo G. Role of lactic acid bacteria during meat conditioning and fermentation Peptides generated as sensorial and hygienic biomarkers. Meat Science. Corea: ScienceDirect; [Internet]. 2010. [Cited 15 oct 2020]. p. 80- 92. Available in: https://www.sciencedirect.com/science/article/abs/pii/S0309174010001555 .Olaoye O, Idowu O. Features and functional properties of lactic acid bacteria used as biological preservatives of meat processing. Journal of Agricultural Technology. [Internet]. 2010. [Cited 18 oct 2020]. 6(3): 449-460. Available in: https://www.researchgate.net/publication/277018649_Features_and_functional_p roperties_of_lactic_acid_bacteria_used_as_biological_preservatives_of_meat_pr ocessing_A_review_article Carpenter C, Smith J, Broadbent J. Efficacy of washing meat surfaces with 2% levulinic, acetic, or lactic acid for pathogen decontamination and residual growth inhibition. ScienceDirect. [Internet]. 2011. [Cited 18 oct 2020]. 88(2): 213-218. Available in: https://ezproxy.unicolmayor.edu.co:2163/science/article/pii/S0309174010004626 Rawdkuen S, Suthiluk P, Kamhangwong D, Benjakul S. Antimicrobial activity of some potential active compounds against food spoilage microorganisms. AJOB. [Internet]. 2012. [Cited 18 oct 2020]. 11(74). Available in: https://www.ajol.info/index.php/ajb/article/view/129338 Chaves R, Silva A, SantAna A, Campana F, Massaguer P. Gas‐ producing and spoilage potential of Enterobacteriaceae and lactic acid bacteria isolated from chilled vacuum‐ packaged beef. International Journal of Food Science and Technology. [Internet]. 2012. [Cited 25 oct 2020]. Available in: https://www.researchgate.net/publication/263728451_Gasproducing_and_spoilage_potential_of_Enterobacteriaceae_and_lactic_acid_bact eria_isolated_from_chilled_vacuum-packaged_beef .Ferrocino I, La Storia A, Torrieri E. Antimicrobial Packaging to Retard the Growth of Spoilage Bacteria and To Reduce the Release of Volatile Metabolites in Meat Stored under Vacuum at 1°C. Journal of food protection. [Internet]. 2013. [Cited 25 oct 2020]. 76(1). Available in: https://meridian.allenpress.com/jfp/article/76/1/52/173850/AntimicrobialPackaging-To-Retard-the-Growth-of .Pu Y, Wang W, Alfano R.Optical Detection of Meat Spoilage Using Fluorescence Spectroscopy with Selective Excitation Wavelength. Sage Journals. [Internet]. 2013. [Cited 25 oct 2020]. 67(2). Available in: https://www.researchgate.net/publication/236457695_Optical_Detection_of_Meat_Spoilage_Using_Fluorescence_Spectroscopy_with_Selective_Excitation_Wavel ength Cizeikiene D, Juodeikiene G, Paskevicius A, Bartkiene E. Antimicrobial activity of lactic acid bacteria against pathogenic and spoilage microorganisms isolated from food and their control in wheat bread. Food control. [Internet].2013. [Cited 28 oct 2020]. 31(2). Available in: https://www.sciencedirect.com/science/article/abs/pii/S0956713512006561 8.Miaoyun L, Tian L, Zhao G, Zhang Q, Gao X, Huang X, et al. Formation of biogenic amines and growth of spoilage-related microorganisms in pork stored under different packaging conditions applying PCA. Meat Science. [Internet]. 2014. [Cited 28 oct 2020]. 96:843-848. Available in: https://www.sciencedirect.com/science/article/abs/pii/S030917401300541X Han K, Hong Y, Kwak H, Kim E, Kim M, Shrivastav A, et al. Identification of Lactic Acid Bacteria in Pork Meat and Pork Meat Products Using SDS‐ PAGE, 16S rRNA Gene Sequencing and MALDI‐ TOF Mass Spectrometry. Wiley Library. [Internet]. 2014. [Cited 28 oct 2020]. 34(3). Available in: https://onlinelibrary.wiley.com/doi/abs/10.1111/jfs.12117 Skariyachan S, Govindarajan S. Biopreservation potential of antimicrobial protein producing Pediococcus spp. towards selected food samples in comparison with chemical preservatives. ScienceDirect. [Internet]. 2019. [Cited 30 oct 2020]; Vol 291: 189-196. Available in: https://doi.org/10.1016/j.ijfoodmicro.2018.12.002 Moreira dos Santos, Wagner L, Aislamiento y caracterización parcial de una bacteriocina producida por Pediococcus sp 347 de origen cárnico. [Internet]. 2020. [Cited 30 oct 2020]. Available in: https://eprints.ucm.es/id/eprint/3192/ Peña M, Bravo J. Aislamiento y purificación de bacteriocinas a partir de Lactobacillus plantarum para su uso como conservantes en carne de res. RRAAE. [Internet]. 2021. [Cited 20 oct 2020]. Available in: https://dspace.uazuay.edu.ec/handle/datos/10459 Salcedo M, Ricardo M. Efecto antagónico de bacterias ácido lácticas aisladas de Tocosh sobre Listeria innocua. BAN. [Internet]. 2021. [Cited 20 oct 2020]. Available in: http://repositorio.lamolina.edu.pe/handle/20.500.12996/4818 Salazar M, Catagña R. Revisión bibliográfica sobre las bacteriocinas y su aplicación como bioconservante dentro de la industria alimentaria. UNACH. [Internet]. 2022. [Cited 20 oct 2020]. Available in: http://dspace.unach.edu.ec/handle/51000/8643 .ENFERMEDADES TRANSMITIDAS POR ALIMENTOS COLOMBIA. [Internet]. 2017. [Cited 25 april 2021]. Available in: https://www.ins.gov.co/buscadoreventos/Informesdeevento/ETA%202017.pdf Mead G. Microbiological quality of poultry meat: a review. Revista Brasileira de Ciência Avícola. [Internet]. 2004. [Cited 25 april 2021]; 6(3):135-142. Available in:https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516- 635X2004000300001&lng=en&nrm=iso&tlng=en Kim M-, Lim T-, Jang J-, Lee D-, Kim B-, Kwon J-, et al. Prevalence and antimicrobial resistance of Salmonella species isolated from chicken meats produced by different integrated broiler operations in Korea. ELSEVIER. [Internet]. 2012. [Cited 25 april 2021]; 91(9):2370-2375. Available in: https://www.sciencedirect.com/science/article/pii/S0032579119396993 Moura G, Tomborelli P, Carvalho RCT, Sigarini C, Carvalho F, Vieira B, et al. Listeria monocytogenes and Other Species as Persistent Contaminants in the Processing of Chicken Meat. Journal of Applied Poultry Research. [Internet]. 2019. [Cited 10 june 2021]; 28(2):470-478. Available in: https://www.researchgate.net/publication/330990775_Listeria_monocytogenes_a nd_Other_Species_as_Persistent_Contaminants_in_the_Processing_of_Chicken _Meat Castellanos R. Liliana C., Villamil J. Luis C., Romero P. Jaime R. Incorporación del Sistema de Análisis de Peligros y Puntos Críticos de Control en la legislación alimentaria. Rev. salud pública. [Cited 10 june 2021]; 6( 3 ): 289-301. Available in:http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0124- 00642004000300005&lng=en. Caballero B. Preservation. Encyclopedia of Food Sciences and Nutrition. Elsevier. [Internet]. 2003. [Cited 10 june 2021]; 3772-7. Available in: https://agris.fao.org/agris-search/search.do?recordID=US201300082710 Ministerio de Salud. Enfermedades transmitidas por alimentos ETA. [Internet]. 2019. [Cited 22 july 2021]. Available in: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/abe ce-eta-final.pdf Pal M, Devrani M. Application of various techniques for meat preservation. Journal of Experimental Food Chemistry. [Internet]. 2018. [Cited 22 july 2021]; Available in: https://www.researchgate.net/publication/322852341_Application_of_Various_Te chniques_for_Meat_Preservation .Vásquez M, Suárez M, Zapata B. UTILIZACIÓN DE SUSTANCIAS ANTIMICROBIANAS PRODUCIDAS POR BACTERIAS ÁCIDO LÁCTICAS EN LA CONSERVACIÓN DE LA CARNE. Rev. chil. [Internet]. 2009. [cited 03 May 2021]; 36(1): 64-71. Available in: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717- 75182009000100007&lng=es. Mills S, Ross P, Hill C. Bacteriocins and bacteriophage; a narrow-minded approach to food and gut microbiology. FEMS Microbiology Reviews. [Internet]. 2017. [Cited 03 May 2021]; Vol 41(1), 129–153. Available in: https://doi.org/10.1093/femsre/fux022 Aguilar C, et al. Emerging strategies for the development of food industries. Taylor&Francis. [Internet]. 2019. [cited 05 sep 2021]; 10(1) 522-537. Available in: https://www.tandfonline.com/doi/full/10.1080/21655979.2019.1682109 Cotter P, Ross R, Hill, C. Bacteriocins a viable alternative to antibiotics? Nature.[Internet]. 2012. [cited 05 sep 2021] 11(2), 95–105. Available in: https://www.nature.com/articles/nrmicro2937 .García L, Comstock L. Bacterial antagonism in host-associated microbial communities. Science.[Internet]. 2018. [cited 05 sep 2021]; 361(6408):1215. Available in: https://science.sciencemag.org/content/361/6408/eaat2456.long .Mondragón G, Escalente P, Osuna J, Ibarra V, Morlett J, Aguilar C, et al., Bacteriocinas: características y aplicación en alimentos. Investigación y Ciencia de la Universidad Autónoma de Aguascalientes. [Internet]. 2013. [cited 05 sep 2021] ; 36(1): 64-71. Disponible en: https://www.redalyc.org/pdf/674/67430113008.pdf Menconi A, Kallapura G, Latorre J, Morgan M, Pumford N, Tellez G, et al. Identification and Characterization of Lactic Acid Bacteria in a Commercial Probiotic Culture. Bioscience of Microbiota, Food and Health. [Internet]. 2014. [Cited 05 sep 2021]; Vol. 33(1), 25-30 Available in: https://www.jstage.jst.go.jp/article/bmfh/33/1/33_bmfh-2013-014/_pdf/-char/ja .Stiles M, Holpzafel W. Lactic acid bacteria of foods and their current taxonomy ELSEVIER. [Internet]. 1997. [Cited 05 sep 2021]. Vol 36 (1), 1-29. Available in: https://www.sciencedirect.com/science/article/abs/pii/S0168160596012330S Faruk T, Ray B. Lactic Acid Bacteria. Current Advances in Metabolism, Genetics and applications. Springer. [Internet]. 1995. [Cited 28 nov 2021]. Vol 98. Available in: https://books.google.com.co/books?id=eK3tCAAAQBAJ&printsec=frontcover#v= onepage&q&f=false Ramirez J, Ulloa P, Velázquez M, Ulloa J, Romero F. Bacterias lácticas: importancia en los alimentos y sus efectos en la salud. [Internet]. 2011. [Cited 28 january 2022]; 2(7):1-13. Available in: http://fuente.uan.edu.mx/publicaciones/03- 07/1.pdf Londoño N. Estado del arte de la obtención de bacteriocinas a partir de bacterias ácido lácticas y su aplicación en la industria de alimentos. [Internet]. 2013. [Cited 28 february 2022]. Available in: https://repository.upb.edu.co/bitstream/handle/20.500.11912/1409/Trabajo%20fin al.pdf?sequence=1 Kumaruiya R, et al. Clasificación de las bacteriocinas de acuerdo a sus características y microorganismos que la producen. [Internet]. 2019. [Cited 26 march 2022]. Available in: https://www.sciencedirect.com/science/article/abs/pii/S088240101831828X?via% 3Dihub Hugas M. Bacteriocinogenic lactic acid bacteria for the biopreservation of meat and meat products. Meat Sci. [Internet]. 1998. [Cited 30 march 2022]; 49:139– 50. Available in: https://www.sciencedirect.com/science/article/abs/pii/S0309174098900444 .Heredia P, Hernandez A, González F, Vallejo B. Bacteriocinas de bacterias ácido lácticas: mecanismos de acción y actividad antimicrobiana contra patógenos en quesos. Interciencia. [Internet]. 2017. [Cited 30 march 2022]. vol 42(6), pp 340- 346. Available in: https://www.redalyc.org/journal/339/33951621002/html/ Olaoye O. Characteristics of lactic acid bacteria being proposed as starter cultures for extending the shelf life of a Nigerian grilled meat product tsire. Asian Journal of Science and Technology. [Internet]. 2014. [Cited 30 march 2020]. Available in: https://www.researchgate.net/publication/277021327_CHARACTERISTICS_OF_ LACTIC_ACID_BACTERIA_BEING_PROPOSED_AS_STARTER_CULTURES_ FOR_EXTENDING_THE_SHELF_LIFE_OF_A_NIGERIAN_GRILLED_MEAT_P RODUCT_TSIRE .Britania. MR Agar. Britanialab. [Internet]. 2017. [cited 29 march 2022]. Available in:https://www.britanialab.com/back/public/upload/productos/upl_6092dd2543f1d. pdf Gaspar C, Donders G, Palmeira R, et al. Bacteriocin production of the probiotic Lactobacillus acidophilus KS400. [Internet]. 2018. [Cited 16 march 2022]; 8, 153 Available in: https://amb-express.springeropen.com/articles/10.1186/s13568-018- 0679-z Cavalieri I, Stephen J. Manual de pruebas de susceptibilidad microbiana. [Internet]. 2018. [Cited 24 march 2022]. Available in: https://www.paho.org/hq/dmdocuments/2005/susceptibilidad-antimicrobianamanual-pruebas-2005.pdf Barcenilla C, Ducix M, Lopez M, Prieto M, Alvarez Ordoñez A. Application of lactic acid bacteria for the biopreservation of meat products: A systematic review. [Internet]. 2021 [Cited 27 march 2022];183 Available in: https://www.sciencedirect.com/science/article/abs/pii/S0168160518309231?via3 Dihub Ramirez J,Rosas P, Velásquez M, Ulloa J, Arce F. Bacterias lácticas: Importancia en alimentos y sus efectos en la salud. Aramara. [Internet]. 2013. [Cited 30 march 2022]. Available in: http://dspace.uan.mx:8080/xmlui/handle/123456789/436 OPS. Resistencia antimicrobiana. [Internet]. 2016. [Cited 29 march 2022]. Available in: https://www.who.int/es/news-room/fact-sheets/detail/antimicrobialresistance .Soltani S, Hammami R, Cotter P, Rebuffat S, Said L, Gaudreau H et al. Bacteriocins as a new generation of antimicrobials: toxicity aspects and regulations. FEMS Microbiology Reviews. [Internet]. 2020; [Cited 30 march 2022]. 45(1). Available in: https://pubmed.ncbi.nlm.nih.gov/32876664/ Arkoun M, Daigle F, Heuzey M, Ajji A. Mechanism of Action of Electrospun Chitosan-Based Nanofibers against Meat Spoilage and Pathogenic Bacteria. Molecules. Pubmed. [Internet]. 2017. [Cited 30 march 2022];22(4):585. Available in: https://pubmed.ncbi.nlm.nih.gov/28383516/ Sánchez M, Salgado M, Hernandez A, Pachon J, Rodríguez E, et al. Nisina (N 234), aditivo utilizado como conservante en alimentos. Gac Med Bilbao [Internet]. 2019. [Cited 16 may 2022];116(3):166-173. Available in: http://www.gacetamedicabilbao.eus/index.php/gacetamedicabilbao/article/view/7 18/726 González B. Detección, purificación y estudio del modo de acción de la plantaricina c; una bacteria producida por una cepa de lactobacillus plantarum aislada del queso de cabrales. Repositorio Institucional de la Universidad de Oviedo. [Internet]. 2012. [Cited 16 may 2022]. Available in: https://digibuo.uniovi.es/dspace/handle/10651/16358 Rivas F, Castro M, Campos C. Efectividad de la bacteriocina sakacina Q aplicada mediante inmersión sobre salchichas tipo Viena. La industria cárnica Latinoamericana. [Internet]. 2015. [Cited 16 may 2022]; 192: 32-36. Available in: http://wwww.publitec.com.ar/contenido/objetos/Efectividaddelabacteriocinasakaci naQaplicadamedianteinmersion.pdf |
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Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022 |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) |
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Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022 https://creativecommons.org/licenses/by-nc-sa/4.0/ Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) http://purl.org/coar/access_right/c_14cb |
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Universidad Colegio Mayor de Cundinamarca |
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Facultad de Ciencias de la Salud |
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Bogotá |
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Bacteriología y Laboratorio Clínico |
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Lancheros Díaz, Ana Gracielac3f677a2bf6df1bd35fb1f073fb3036b600Diaz Cañón, Yenny Alejandra6cbe58e1e1110babe851b2f35fa37417Salgado Gasca, Jeimi Tatianaef9fe1dd761c8256d920aa91f26ef7446002022-09-15T16:16:14Z2022-09-15T16:16:14Z2022https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5670La conservación de las proteínas de origen animal , representa un reto para la industria alimentaria, ya que, además de asegurar la inocuidad del producto, de igual forma debe reducir al máximo las pérdidas económicas que puedan presentarse en consecuencia. 1 La presente monografía pretende fundamentar la importancia de nuevas alternativas para la conservación de carnes, haciendo uso de bacterias ácido lácticas (BAL), microorganismos habitualmente utilizados cómo probióticos en diferentes alimentos, gracias a que poseen mecanismos prometedores en funciones de biopreservación. Para ello, la presente revisión bibliográfica realizada en bases datos de carácter científicoacadémico tales cómo Scopus, ScienceDirect, Nature, ELSEVIER, Multidisciplinary Digital Publishing Institute (MDPI), Pubmed, Taylor and Francis; destaca diferentes mecanismos, tales cómo la producción de ácido láctico, ácido acético, peróxido de Hidrógeno, dióxido de Carbono, así mismo la producción de metabolitos propios, dentro de ellos las bacteriocinas que utilizadas de forma individual o en conjunto tienen una alta versatilidad en la industria, agregando el hecho de que dada la naturaleza de estos compuestos, permite describirlos como sustancias seguras para la salud humana, por lo tanto, se deduce el uso de las BAL como potentes agentes microbianos además de ser una alternativa alimentaria segura y rentable.The conservation of proteins of animal origin represents a challenge for the food industry, since, in addition to ensuring the ubiquity of the product, it must also minimize the economic losses that may arise as a result.1 This monograph aims to substantiate the importance of new alternatives for meat preservation, making use of lactic acid bacteria (LAB), microorganisms commonly used as probiotics in different foods, thanks to their promising mechanisms in biopreservation functions. For this, the present bibliographic review carried out in scientific-academic databases such as Scopus, ScienceDirect, Nature, ELSEVIER, Multidisciplinary Digital Publishing Institute (MDPI), Pubmed, Taylor and Francis; highlights different mechanisms, such as the production of lactic acid, acetic acid, hydrogen peroxide, carbon dioxide, as well as the production of their own metabolites, within them the bacteriocins that used individually or together have a high versatility in the industry, adding the fact that given the nature of these compounds, it allows to describe them as safe substances for human health, therefore, the use of LAB as powerful microbial agents is deduced in addition to being a safe and profitable food alternative.RESUMEN 6 ANTECEDENTES 9 MARCO REFERENCIAL 19 2.1 Microbiota patógena de carne de pollo 19 2.2 Industria alimentaria y Salud Pública 20 2.3 Conservación tradicional de las carnes en la industria alimentaria 21 2.4 Bioconservación en la industria alimentaria 22 2.5 Antagonismo bacteriano 23 2.6 BAL cómo una innovación en la calidad microbiológica. 23 2.7 Mecanismos de acción de BAL 25 2.7.1 Bacterias Ácido Lácticas Homofermentativas 25 2.7.2 Bacterias Ácido Lácticas Heterofermentativas 25 2.8 Mecanismos de BAL: Bacteriocinas 26 2.8.1 Producción de las Bacteriocinas 29 2.8.2 Identificación de las obtencion de bacteriocinas 31 2.9 Bacteriocinas y su papel dentro de la industria alimentaria 32 2.10 Resistencia a las bacteriocinas 33 OBJETIVOS 34 3.1 Objetivo general 34 3.2 Objetivos específicos 34 DISEÑO METODOLÓGICO 35 4.1. Tipo de investigación 35 4.1.1 Nivel o enfoque investigativo 35 4.1.2 Población objeto de estudio 35 4.1.3 Muestra 36 4.2 Estrategias de búsqueda 36 4.3 Criterios de Inclusión y exclusión 36 4.4 Hipótesis 37 5. RESULTADOS 37 5.1 Uso de las BAL y sus mecanismos dentro de la industria alimentaria 37 5.2. Propuesta de protocolo para el uso de bacteriocinas dentro de la industria cárnica 42 5.2.1 Protocolo Ex Situ 43 5.2.2 Protocolo In Situ 44 6. DISCUSIÓN 45 7. CONCLUSIONES 49PregradoBacteriólogo(a) y Laboratorista Clínico71p.application/pdfspaUniversidad Colegio Mayor de CundinamarcaFacultad de Ciencias de la SaludBogotáBacteriología y Laboratorio ClínicoDerechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/closedAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_14cbBacterias ácido lácticas como bioconservantes en carnes en la industria alimentariaTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Textinfo:eu-repo/semantics/bachelorThesishttps://purl.org/redcol/resource_type/TPinfo:eu-repo/semantics/publishedVersionThyagarajan D, Punniamurthy N, Bhaisare DB, Thyagarajan D, Churchil RR, Punniamurthy N. Bacterial Pathogens in Chicken Meat: Review. Print International Journal of Life Sciences Research. [Internet]. 2014. [Cited 20 nov 2020]; 2(3): 1-7. Available in: https://www.researchgate.net/profile/RChurchil/publication/347510610_Bacterial_Pathogens_in_Chicken_Meat_Review /links/5fdf431f45851553a0d65825/Bacterial-Pathogens-in-Chicken-MeatReview.pdfInformación estadística - FENAVI - Federación Nacional de Avicultores de Colombia. [Cited 20 nov 2020]. Available in: https://fenavi.org/informacionestadistica/Informacion estadística-FEDEGAN-Federación Colombiana de Ganaderos. [Cited 20 nov 2020]. Available in: https://www.fedegan.org.co/estadisticas/consumo-0. Bintsis T. Lactic acid bacteria: their applications in foods . J Bacteriol Mycol Open Access. [Internet]. 2018. [Cited 14 oct 2020];6(2):89–94. Available in: 10.15406/jbmoa.2018.06.00182Gálves A, López R, Abriouel H, Valdivia A, Ben N. Application of Bacteriocins in the Control of Foodborne Pathogenic and Spoilage Bacteria. Revista Taylor & Francis. [Internet]. 2008. [Cited 14 oct 2020]; 28(2). Available in : https://www.tandfonline.com/doi/full/10.1080/07388550802107202Saddam A, Salam I. Screening of Antibacterial Activity of Lactic Acid Bacteria Against Different Pathogens Found in Vacuum-Packaged Meat Products. Revista Foodborne Pathogens and disease. [Internet].2009. [Cited 15 oct 2020]; 6(9). Available in: https://www.liebertpub.com/doi/10.1089/fpd.2009.0272?url_ver=Z39.88- 2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmedNorrung B, Andersen J, Buncic S. Main Concerns of Pathogenic Microorganisms in Meat. Recopilado del libro: Safety of meat and processed. España: Springer; 2009. [Internet]. [Cited 20 nov 2020]. Available in: https://link.springer.com/chapter/10.1007/978-0-387-89026-5_1Nychas J, Dourou D, Skandamis P, Koutsoumanis K, Baranyi J, Sofos J. Effect of microbial cell‐ free meat extract on the growth of spoilage bacteria. Journal of Applied Microbiology. [Internet]. 2009. [Cited 14 oct 2020]; 1819-1829. Available in: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365- 2672.2009.04377.xErcolini D, Russo F, Nasi A, Ferranti P, Villani F. Mesophilic and Psychrotrophic Bacteria from Meat and Their Spoilage Potential in vitro and in beef. Potential in Vitro and in Beef. American Society for Microbiology. [Internet]. 2009. [Cited 15 oct 2020]; 75(7). Available in: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663181/Fadda S, López C, Vignolo G. Role of lactic acid bacteria during meat conditioning and fermentation Peptides generated as sensorial and hygienic biomarkers. Meat Science. Corea: ScienceDirect; [Internet]. 2010. [Cited 15 oct 2020]. p. 80- 92. Available in: https://www.sciencedirect.com/science/article/abs/pii/S0309174010001555.Olaoye O, Idowu O. Features and functional properties of lactic acid bacteria used as biological preservatives of meat processing. Journal of Agricultural Technology. [Internet]. 2010. [Cited 18 oct 2020]. 6(3): 449-460. Available in: https://www.researchgate.net/publication/277018649_Features_and_functional_p roperties_of_lactic_acid_bacteria_used_as_biological_preservatives_of_meat_pr ocessing_A_review_articleCarpenter C, Smith J, Broadbent J. Efficacy of washing meat surfaces with 2% levulinic, acetic, or lactic acid for pathogen decontamination and residual growth inhibition. ScienceDirect. [Internet]. 2011. [Cited 18 oct 2020]. 88(2): 213-218. Available in: https://ezproxy.unicolmayor.edu.co:2163/science/article/pii/S0309174010004626Rawdkuen S, Suthiluk P, Kamhangwong D, Benjakul S. Antimicrobial activity of some potential active compounds against food spoilage microorganisms. AJOB. [Internet]. 2012. [Cited 18 oct 2020]. 11(74). Available in: https://www.ajol.info/index.php/ajb/article/view/129338Chaves R, Silva A, SantAna A, Campana F, Massaguer P. Gas‐ producing and spoilage potential of Enterobacteriaceae and lactic acid bacteria isolated from chilled vacuum‐ packaged beef. International Journal of Food Science and Technology. [Internet]. 2012. [Cited 25 oct 2020]. Available in: https://www.researchgate.net/publication/263728451_Gasproducing_and_spoilage_potential_of_Enterobacteriaceae_and_lactic_acid_bact eria_isolated_from_chilled_vacuum-packaged_beef.Ferrocino I, La Storia A, Torrieri E. Antimicrobial Packaging to Retard the Growth of Spoilage Bacteria and To Reduce the Release of Volatile Metabolites in Meat Stored under Vacuum at 1°C. Journal of food protection. [Internet]. 2013. [Cited 25 oct 2020]. 76(1). Available in: https://meridian.allenpress.com/jfp/article/76/1/52/173850/AntimicrobialPackaging-To-Retard-the-Growth-of.Pu Y, Wang W, Alfano R.Optical Detection of Meat Spoilage Using Fluorescence Spectroscopy with Selective Excitation Wavelength. Sage Journals. [Internet]. 2013. [Cited 25 oct 2020]. 67(2). Available in: https://www.researchgate.net/publication/236457695_Optical_Detection_of_Meat_Spoilage_Using_Fluorescence_Spectroscopy_with_Selective_Excitation_Wavel engthCizeikiene D, Juodeikiene G, Paskevicius A, Bartkiene E. Antimicrobial activity of lactic acid bacteria against pathogenic and spoilage microorganisms isolated from food and their control in wheat bread. Food control. [Internet].2013. [Cited 28 oct 2020]. 31(2). Available in: https://www.sciencedirect.com/science/article/abs/pii/S09567135120065618.Miaoyun L, Tian L, Zhao G, Zhang Q, Gao X, Huang X, et al. Formation of biogenic amines and growth of spoilage-related microorganisms in pork stored under different packaging conditions applying PCA. Meat Science. [Internet]. 2014. [Cited 28 oct 2020]. 96:843-848. Available in: https://www.sciencedirect.com/science/article/abs/pii/S030917401300541XHan K, Hong Y, Kwak H, Kim E, Kim M, Shrivastav A, et al. Identification of Lactic Acid Bacteria in Pork Meat and Pork Meat Products Using SDS‐ PAGE, 16S rRNA Gene Sequencing and MALDI‐ TOF Mass Spectrometry. Wiley Library. [Internet]. 2014. [Cited 28 oct 2020]. 34(3). Available in: https://onlinelibrary.wiley.com/doi/abs/10.1111/jfs.12117Skariyachan S, Govindarajan S. Biopreservation potential of antimicrobial protein producing Pediococcus spp. towards selected food samples in comparison with chemical preservatives. ScienceDirect. [Internet]. 2019. [Cited 30 oct 2020]; Vol 291: 189-196. Available in: https://doi.org/10.1016/j.ijfoodmicro.2018.12.002Moreira dos Santos, Wagner L, Aislamiento y caracterización parcial de una bacteriocina producida por Pediococcus sp 347 de origen cárnico. [Internet]. 2020. [Cited 30 oct 2020]. Available in: https://eprints.ucm.es/id/eprint/3192/Peña M, Bravo J. Aislamiento y purificación de bacteriocinas a partir de Lactobacillus plantarum para su uso como conservantes en carne de res. RRAAE. [Internet]. 2021. [Cited 20 oct 2020]. Available in: https://dspace.uazuay.edu.ec/handle/datos/10459Salcedo M, Ricardo M. Efecto antagónico de bacterias ácido lácticas aisladas de Tocosh sobre Listeria innocua. BAN. [Internet]. 2021. [Cited 20 oct 2020]. Available in: http://repositorio.lamolina.edu.pe/handle/20.500.12996/4818Salazar M, Catagña R. Revisión bibliográfica sobre las bacteriocinas y su aplicación como bioconservante dentro de la industria alimentaria. UNACH. [Internet]. 2022. [Cited 20 oct 2020]. Available in: http://dspace.unach.edu.ec/handle/51000/8643.ENFERMEDADES TRANSMITIDAS POR ALIMENTOS COLOMBIA. [Internet]. 2017. [Cited 25 april 2021]. Available in: https://www.ins.gov.co/buscadoreventos/Informesdeevento/ETA%202017.pdfMead G. Microbiological quality of poultry meat: a review. Revista Brasileira de Ciência Avícola. [Internet]. 2004. [Cited 25 april 2021]; 6(3):135-142. Available in:https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516- 635X2004000300001&lng=en&nrm=iso&tlng=enKim M-, Lim T-, Jang J-, Lee D-, Kim B-, Kwon J-, et al. Prevalence and antimicrobial resistance of Salmonella species isolated from chicken meats produced by different integrated broiler operations in Korea. ELSEVIER. [Internet]. 2012. [Cited 25 april 2021]; 91(9):2370-2375. Available in: https://www.sciencedirect.com/science/article/pii/S0032579119396993Moura G, Tomborelli P, Carvalho RCT, Sigarini C, Carvalho F, Vieira B, et al. Listeria monocytogenes and Other Species as Persistent Contaminants in the Processing of Chicken Meat. Journal of Applied Poultry Research. [Internet]. 2019. [Cited 10 june 2021]; 28(2):470-478. Available in: https://www.researchgate.net/publication/330990775_Listeria_monocytogenes_a nd_Other_Species_as_Persistent_Contaminants_in_the_Processing_of_Chicken _MeatCastellanos R. Liliana C., Villamil J. Luis C., Romero P. Jaime R. Incorporación del Sistema de Análisis de Peligros y Puntos Críticos de Control en la legislación alimentaria. Rev. salud pública. [Cited 10 june 2021]; 6( 3 ): 289-301. Available in:http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0124- 00642004000300005&lng=en.Caballero B. Preservation. Encyclopedia of Food Sciences and Nutrition. Elsevier. [Internet]. 2003. [Cited 10 june 2021]; 3772-7. Available in: https://agris.fao.org/agris-search/search.do?recordID=US201300082710Ministerio de Salud. Enfermedades transmitidas por alimentos ETA. [Internet]. 2019. [Cited 22 july 2021]. Available in: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/abe ce-eta-final.pdfPal M, Devrani M. Application of various techniques for meat preservation. Journal of Experimental Food Chemistry. [Internet]. 2018. [Cited 22 july 2021]; Available in: https://www.researchgate.net/publication/322852341_Application_of_Various_Te chniques_for_Meat_Preservation.Vásquez M, Suárez M, Zapata B. UTILIZACIÓN DE SUSTANCIAS ANTIMICROBIANAS PRODUCIDAS POR BACTERIAS ÁCIDO LÁCTICAS EN LA CONSERVACIÓN DE LA CARNE. Rev. chil. [Internet]. 2009. [cited 03 May 2021]; 36(1): 64-71. Available in: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717- 75182009000100007&lng=es.Mills S, Ross P, Hill C. Bacteriocins and bacteriophage; a narrow-minded approach to food and gut microbiology. FEMS Microbiology Reviews. [Internet]. 2017. [Cited 03 May 2021]; Vol 41(1), 129–153. Available in: https://doi.org/10.1093/femsre/fux022Aguilar C, et al. Emerging strategies for the development of food industries. Taylor&Francis. [Internet]. 2019. [cited 05 sep 2021]; 10(1) 522-537. Available in: https://www.tandfonline.com/doi/full/10.1080/21655979.2019.1682109Cotter P, Ross R, Hill, C. Bacteriocins a viable alternative to antibiotics? Nature.[Internet]. 2012. [cited 05 sep 2021] 11(2), 95–105. Available in: https://www.nature.com/articles/nrmicro2937.García L, Comstock L. Bacterial antagonism in host-associated microbial communities. Science.[Internet]. 2018. [cited 05 sep 2021]; 361(6408):1215. Available in: https://science.sciencemag.org/content/361/6408/eaat2456.long.Mondragón G, Escalente P, Osuna J, Ibarra V, Morlett J, Aguilar C, et al., Bacteriocinas: características y aplicación en alimentos. Investigación y Ciencia de la Universidad Autónoma de Aguascalientes. [Internet]. 2013. [cited 05 sep 2021] ; 36(1): 64-71. Disponible en: https://www.redalyc.org/pdf/674/67430113008.pdfMenconi A, Kallapura G, Latorre J, Morgan M, Pumford N, Tellez G, et al. Identification and Characterization of Lactic Acid Bacteria in a Commercial Probiotic Culture. Bioscience of Microbiota, Food and Health. [Internet]. 2014. [Cited 05 sep 2021]; Vol. 33(1), 25-30 Available in: https://www.jstage.jst.go.jp/article/bmfh/33/1/33_bmfh-2013-014/_pdf/-char/ja.Stiles M, Holpzafel W. Lactic acid bacteria of foods and their current taxonomy ELSEVIER. [Internet]. 1997. [Cited 05 sep 2021]. Vol 36 (1), 1-29. Available in: https://www.sciencedirect.com/science/article/abs/pii/S0168160596012330SFaruk T, Ray B. Lactic Acid Bacteria. Current Advances in Metabolism, Genetics and applications. Springer. [Internet]. 1995. [Cited 28 nov 2021]. Vol 98. Available in: https://books.google.com.co/books?id=eK3tCAAAQBAJ&printsec=frontcover#v= onepage&q&f=falseRamirez J, Ulloa P, Velázquez M, Ulloa J, Romero F. Bacterias lácticas: importancia en los alimentos y sus efectos en la salud. [Internet]. 2011. [Cited 28 january 2022]; 2(7):1-13. Available in: http://fuente.uan.edu.mx/publicaciones/03- 07/1.pdfLondoño N. Estado del arte de la obtención de bacteriocinas a partir de bacterias ácido lácticas y su aplicación en la industria de alimentos. [Internet]. 2013. [Cited 28 february 2022]. Available in: https://repository.upb.edu.co/bitstream/handle/20.500.11912/1409/Trabajo%20fin al.pdf?sequence=1Kumaruiya R, et al. Clasificación de las bacteriocinas de acuerdo a sus características y microorganismos que la producen. [Internet]. 2019. [Cited 26 march 2022]. Available in: https://www.sciencedirect.com/science/article/abs/pii/S088240101831828X?via% 3DihubHugas M. Bacteriocinogenic lactic acid bacteria for the biopreservation of meat and meat products. Meat Sci. [Internet]. 1998. [Cited 30 march 2022]; 49:139– 50. Available in: https://www.sciencedirect.com/science/article/abs/pii/S0309174098900444.Heredia P, Hernandez A, González F, Vallejo B. Bacteriocinas de bacterias ácido lácticas: mecanismos de acción y actividad antimicrobiana contra patógenos en quesos. Interciencia. [Internet]. 2017. [Cited 30 march 2022]. vol 42(6), pp 340- 346. Available in: https://www.redalyc.org/journal/339/33951621002/html/Olaoye O. Characteristics of lactic acid bacteria being proposed as starter cultures for extending the shelf life of a Nigerian grilled meat product tsire. Asian Journal of Science and Technology. [Internet]. 2014. [Cited 30 march 2020]. Available in: https://www.researchgate.net/publication/277021327_CHARACTERISTICS_OF_ LACTIC_ACID_BACTERIA_BEING_PROPOSED_AS_STARTER_CULTURES_ FOR_EXTENDING_THE_SHELF_LIFE_OF_A_NIGERIAN_GRILLED_MEAT_P RODUCT_TSIRE.Britania. MR Agar. Britanialab. [Internet]. 2017. [cited 29 march 2022]. Available in:https://www.britanialab.com/back/public/upload/productos/upl_6092dd2543f1d. pdfGaspar C, Donders G, Palmeira R, et al. Bacteriocin production of the probiotic Lactobacillus acidophilus KS400. [Internet]. 2018. [Cited 16 march 2022]; 8, 153 Available in: https://amb-express.springeropen.com/articles/10.1186/s13568-018- 0679-zCavalieri I, Stephen J. Manual de pruebas de susceptibilidad microbiana. [Internet]. 2018. [Cited 24 march 2022]. Available in: https://www.paho.org/hq/dmdocuments/2005/susceptibilidad-antimicrobianamanual-pruebas-2005.pdfBarcenilla C, Ducix M, Lopez M, Prieto M, Alvarez Ordoñez A. Application of lactic acid bacteria for the biopreservation of meat products: A systematic review. [Internet]. 2021 [Cited 27 march 2022];183 Available in: https://www.sciencedirect.com/science/article/abs/pii/S0168160518309231?via3 DihubRamirez J,Rosas P, Velásquez M, Ulloa J, Arce F. Bacterias lácticas: Importancia en alimentos y sus efectos en la salud. Aramara. [Internet]. 2013. [Cited 30 march 2022]. Available in: http://dspace.uan.mx:8080/xmlui/handle/123456789/436OPS. Resistencia antimicrobiana. [Internet]. 2016. [Cited 29 march 2022]. Available in: https://www.who.int/es/news-room/fact-sheets/detail/antimicrobialresistance.Soltani S, Hammami R, Cotter P, Rebuffat S, Said L, Gaudreau H et al. Bacteriocins as a new generation of antimicrobials: toxicity aspects and regulations. FEMS Microbiology Reviews. [Internet]. 2020; [Cited 30 march 2022]. 45(1). Available in: https://pubmed.ncbi.nlm.nih.gov/32876664/Arkoun M, Daigle F, Heuzey M, Ajji A. Mechanism of Action of Electrospun Chitosan-Based Nanofibers against Meat Spoilage and Pathogenic Bacteria. Molecules. Pubmed. [Internet]. 2017. [Cited 30 march 2022];22(4):585. Available in: https://pubmed.ncbi.nlm.nih.gov/28383516/Sánchez M, Salgado M, Hernandez A, Pachon J, Rodríguez E, et al. Nisina (N 234), aditivo utilizado como conservante en alimentos. Gac Med Bilbao [Internet]. 2019. [Cited 16 may 2022];116(3):166-173. Available in: http://www.gacetamedicabilbao.eus/index.php/gacetamedicabilbao/article/view/7 18/726González B. Detección, purificación y estudio del modo de acción de la plantaricina c; una bacteria producida por una cepa de lactobacillus plantarum aislada del queso de cabrales. Repositorio Institucional de la Universidad de Oviedo. [Internet]. 2012. [Cited 16 may 2022]. Available in: https://digibuo.uniovi.es/dspace/handle/10651/16358Rivas F, Castro M, Campos C. Efectividad de la bacteriocina sakacina Q aplicada mediante inmersión sobre salchichas tipo Viena. La industria cárnica Latinoamericana. [Internet]. 2015. [Cited 16 may 2022]; 192: 32-36. 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accessunicolmayor/5670oai:repositorio.unicolmayor.edu.co:unicolmayor/56702024-05-09 12:55:21.324An error occurred on the license name.|||https://creativecommons.org/licenses/by-nc-sa/4.0/open accessBiblioteca Digital Unicolmayorrepositorio@unicolmayor.edu.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