Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander)
The genus Listeria comprises eight species between them Listeria monocytogenes, pathogenic to humans, causing gastroenteritis, meningitis, neuro-encephalitis, septicemia, abortions and even death. L. monocytogenes is an ubiquitous bacterium which can be isolated from ecosystems inhabited by fish, re...
- Autores:
-
Herrera A., Fanny
Suárez Q., William
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/1796
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/823
- Palabra clave:
- Listeria monocytogenes
Pescado
Escherichia coli
Prevalencia
Listeria monocytogenes
Escherichia coli
Pescado
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
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Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander) |
| dc.title.alternative.spa.fl_str_mv |
Isolation and identification of Listeria spp. from fresh fish samples, marketed in Pamplona (Norte de Santander) |
| title |
Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander) |
| spellingShingle |
Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander) Listeria monocytogenes Pescado Escherichia coli Prevalencia Listeria monocytogenes Escherichia coli Pescado |
| title_short |
Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander) |
| title_full |
Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander) |
| title_fullStr |
Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander) |
| title_full_unstemmed |
Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander) |
| title_sort |
Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander) |
| dc.creator.fl_str_mv |
Herrera A., Fanny Suárez Q., William |
| dc.contributor.author.spa.fl_str_mv |
Herrera A., Fanny Suárez Q., William |
| dc.subject.proposal.spa.fl_str_mv |
Listeria monocytogenes Pescado Escherichia coli Prevalencia |
| topic |
Listeria monocytogenes Pescado Escherichia coli Prevalencia Listeria monocytogenes Escherichia coli Pescado |
| dc.subject.agrovoc.spa.fl_str_mv |
Listeria monocytogenes Escherichia coli Pescado |
| description |
The genus Listeria comprises eight species between them Listeria monocytogenes, pathogenic to humans, causing gastroenteritis, meningitis, neuro-encephalitis, septicemia, abortions and even death. L. monocytogenes is an ubiquitous bacterium which can be isolated from ecosystems inhabited by fish, reaching the finished product, even at high concentrations, when the fish is processed using inadequate cleaning and disinfection practices and/or is stored for a long time at refrigeration temperatures because it is psychrotrophic. The objectives of this study were to determine the presence of Listeria spp. in fresh fish samples expended in the market place of Pamplona, comparing the traditional method with an immunochromatografic one, and to verify whether there is a correlation between the presence of Escherichia coli and Listeria spp. in the samples. L. monocytogenes, L. innocuaand L. grayi were isolated, with prevalences of 3.9%, 17.6% and 13.7%, respectively, with an overall incidence of 35.3%. By comparing the isolation methods, traditional and immunochromatografic, no significant differences between them were found. Finally, it was established that the presence of E.coli is a good indicator of the presence of strains of Listeria in the samples. The presence of L. monocytogenesin the samples indicates that fish sold in the market place of Pamplona can be an important vehicle for this pathogen and implies, that this food might pose a health risk to consumers, especially the vulnerable population. |
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2012 |
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2012-07 |
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2019-07-15T20:14:44Z |
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2019-07-15T20:14:44Z |
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Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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Text |
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http://purl.org/redcol/resource_type/ART |
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0123-4226 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/823 |
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305294 |
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0123-4226 305294 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/823 |
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spa |
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spa |
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Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 15, No. 2 (Jul.-Dic. 2012). -- páginas 257-265 |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales |
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Pamplona (Norte de Santander, Colombia) |
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Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2012 |
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Universidad de Ciencias Aplicadas y Ambientales U.D.C.A |
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Herrera A., FannySuárez Q., WilliamPamplona (Norte de Santander, Colombia)2019-07-15T20:14:44Z2019-07-15T20:14:44Z2012-070123-4226https://revistas.udca.edu.co/index.php/ruadc/article/view/823305294The genus Listeria comprises eight species between them Listeria monocytogenes, pathogenic to humans, causing gastroenteritis, meningitis, neuro-encephalitis, septicemia, abortions and even death. L. monocytogenes is an ubiquitous bacterium which can be isolated from ecosystems inhabited by fish, reaching the finished product, even at high concentrations, when the fish is processed using inadequate cleaning and disinfection practices and/or is stored for a long time at refrigeration temperatures because it is psychrotrophic. The objectives of this study were to determine the presence of Listeria spp. in fresh fish samples expended in the market place of Pamplona, comparing the traditional method with an immunochromatografic one, and to verify whether there is a correlation between the presence of Escherichia coli and Listeria spp. in the samples. L. monocytogenes, L. innocuaand L. grayi were isolated, with prevalences of 3.9%, 17.6% and 13.7%, respectively, with an overall incidence of 35.3%. By comparing the isolation methods, traditional and immunochromatografic, no significant differences between them were found. Finally, it was established that the presence of E.coli is a good indicator of the presence of strains of Listeria in the samples. The presence of L. monocytogenesin the samples indicates that fish sold in the market place of Pamplona can be an important vehicle for this pathogen and implies, that this food might pose a health risk to consumers, especially the vulnerable population.El género Listeria contiene ocho especies, destancándose Listeria monocytogenes patógena para el hombre que puede ocasionar gastroenteritis, meningitis, neuro-encefalitis, septicemia, abortos e, incluso, la muerte. L. monocytogenes es una bacteria ubicua que se puede aislar a partir de los ecosistemas donde habitan los peces, logrando llegar al producto terminado e inclusive, a concentraciones elevadas, cuando el pescado se procesa empleando inadecuadas prácticas de limpieza y de desinfección o se conserva durante mucho tiempo a temperaturas de refrigeración, debido a que es un microorganismo psicrotrófico. Los objetivos del presente estudio fueron determinar la presencia de Listeria spp. en muestras de pescado fresco expendido en la plaza de mercado de la ciudad de Pamplona, comparando el método tradicional y un inmunocromatográfico y determinar si existe correlación entre la presencia de Escherichia coli y Listeria spp., en las muestras. Se aislaron L. monocytogenes, L. innocua y L. grayi, con prevalencias del 3,9%, 17,6%, y 13,7%, respectivamente, con una incidencia total del 35,3%. Al comparar los dos métodos de aislamiento, se encontró que no se existieron diferencias significativas entre ellos. Finalmente, se determinó que la presencia de E. coli es un buen indicador de la manifestación de cepas de Listeria en las muestras. La presencia de L. monocytogenes en las muestras indica que el pescado comercializado en la plaza de mercado de Pamplona puede ser un vehículo importante para este patógeno e, implica, que este alimento puede representar un riesgo para la salud de los consumidores, especialmente, para población vulnerable.Incluye referencias bibliográficasapplication/pdfspaBogotá : Universidad de Ciencias Aplicadas y Ambientales, 2012Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 15, No. 2 (Jul.-Dic. 2012). -- páginas 257-265Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientaleshttps://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander)Isolation and identification of Listeria spp. from fresh fish samples, marketed in Pamplona (Norte de Santander)Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTexthttp://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85Listeria monocytogenesPescadoEscherichia coliPrevalenciaListeria monocytogenesEscherichia coliPescadoPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-814775https://repository.udca.edu.co/bitstreams/69c0ceb8-1d79-4019-8f76-b2997760dc46/downloadf661acf14bedbf9f5d13897a0387e751MD52ORIGINAL823-Texto del artículo-2751-1-10-20180907.pdf823-Texto del artículo-2751-1-10-20180907.pdfapplication/pdf775295https://repository.udca.edu.co/bitstreams/9d78d31a-ff31-4494-9bb8-680d7ada5a36/downloadd1580a5f784d1e5eb4efef1877da0e73MD51TEXT823-Texto del artículo-2751-1-10-20180907.pdf.txt823-Texto del artículo-2751-1-10-20180907.pdf.txtExtracted 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 nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

 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