Aislamiento e identificación de Listeria spp. a partir de muestras de pescado fresco expendido en Pamplona (Norte de Santander)

The genus Listeria comprises eight species between them Listeria monocytogenes, pathogenic to humans, causing gastroenteritis, meningitis, neuro-encephalitis, septicemia, abortions and even death. L. monocytogenes is an ubiquitous bacterium which can be isolated from ecosystems inhabited by fish, re...

Full description

Autores:
Herrera A., Fanny
Suárez Q., William
Tipo de recurso:
Article of journal
Fecha de publicación:
2012
Institución:
Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
Repositorio:
Repositorio Institucional UDCA
Idioma:
spa
OAI Identifier:
oai:repository.udca.edu.co:11158/1796
Acceso en línea:
https://revistas.udca.edu.co/index.php/ruadc/article/view/823
Palabra clave:
Listeria monocytogenes
Pescado
Escherichia coli
Prevalencia
Listeria monocytogenes
Escherichia coli
Pescado
Rights
openAccess
License
Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
Description
Summary:The genus Listeria comprises eight species between them Listeria monocytogenes, pathogenic to humans, causing gastroenteritis, meningitis, neuro-encephalitis, septicemia, abortions and even death. L. monocytogenes is an ubiquitous bacterium which can be isolated from ecosystems inhabited by fish, reaching the finished product, even at high concentrations, when the fish is processed using inadequate cleaning and disinfection practices and/or is stored for a long time at refrigeration temperatures because it is psychrotrophic. The objectives of this study were to determine the presence of Listeria spp. in fresh fish samples expended in the market place of Pamplona, comparing the traditional method with an immunochromatografic one, and to verify whether there is a correlation between the presence of Escherichia coli and Listeria spp. in the samples. L. monocytogenes, L. innocuaand L. grayi were isolated, with prevalences of 3.9%, 17.6% and 13.7%, respectively, with an overall incidence of 35.3%. By comparing the isolation methods, traditional and immunochromatografic, no significant differences between them were found. Finally, it was established that the presence of E.coli is a good indicator of the presence of strains of Listeria in the samples. The presence of L. monocytogenesin the samples indicates that fish sold in the market place of Pamplona can be an important vehicle for this pathogen and implies, that this food might pose a health risk to consumers, especially the vulnerable population.