Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L.
The yellowing pea caused by Fusarium oxysporum is the most problem in the crop and its damage to bring economic losses. This research was carried out in the laboratory and greenhouse of the University of Nariño, located at 2486 masl to an average temperature of 13ºC., in order to evaluate the antago...
- Autores:
-
Guerra, Germán Andrés
Betancourth, Carlos Arturo
Salazar, Claudia Elizabeth
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2011
- Institución:
- Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
- Repositorio:
- Repositorio Institucional UDCA
- Idioma:
- spa
- OAI Identifier:
- oai:repository.udca.edu.co:11158/2190
- Acceso en línea:
- https://revistas.udca.edu.co/index.php/ruadc/article/view/773
- Palabra clave:
- Control biológico
Rizobacteria
Incidencia
Amarillamiento
Pseudomonas fluorescens
Fusarium oxysporum f. sp. pisi
Pisum sativum
Control biológico
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales
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Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. |
| dc.title.alternative.spa.fl_str_mv |
Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. |
| title |
Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. |
| spellingShingle |
Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. Control biológico Rizobacteria Incidencia Amarillamiento Pseudomonas fluorescens Fusarium oxysporum f. sp. pisi Pisum sativum Control biológico |
| title_short |
Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. |
| title_full |
Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. |
| title_fullStr |
Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. |
| title_full_unstemmed |
Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. |
| title_sort |
Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L. |
| dc.creator.fl_str_mv |
Guerra, Germán Andrés Betancourth, Carlos Arturo Salazar, Claudia Elizabeth |
| dc.contributor.author.spa.fl_str_mv |
Guerra, Germán Andrés Betancourth, Carlos Arturo Salazar, Claudia Elizabeth |
| dc.subject.proposal.spa.fl_str_mv |
Control biológico Rizobacteria Incidencia Amarillamiento |
| topic |
Control biológico Rizobacteria Incidencia Amarillamiento Pseudomonas fluorescens Fusarium oxysporum f. sp. pisi Pisum sativum Control biológico |
| dc.subject.agrovoc.spa.fl_str_mv |
Pseudomonas fluorescens Fusarium oxysporum f. sp. pisi Pisum sativum Control biológico |
| description |
The yellowing pea caused by Fusarium oxysporum is the most problem in the crop and its damage to bring economic losses. This research was carried out in the laboratory and greenhouse of the University of Nariño, located at 2486 masl to an average temperature of 13ºC., in order to evaluate the antagonism of P. fluorescent facing F. oxysporum on pea Pisum sativum L. The isolation of F. oxysporum affected tissue was made from roots and stems of pea plants, which were multiplied and purified on PDA, strain selection was based on the prevalence in all zones and the speed in their growth, obtaining the strain fTR. The isolation of P. fluorescent was made from roots and stems of healthy pea plants that were in focus with yellowing, which were purified and multiplied in Selective Cetrimide Agar, obtaining strains PPC, PPO and PPL; after biochemical tests. The tests were made in dual in medium Nutrient Agar, in which the strain showed higher PPC antagonism to control the growth of F. oxysporum under In vitro conditions in the laboratory. In greenhouse tests the incidence rose from 90.31% to 6.55% with the bacteria compared with the control, showing the efficiency of the antagonistic strain P. fluorescens PPC in the control of F. oxysporum under controlled conditions. |
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2011 |
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2011-07 |
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2019-09-30T15:53:37Z |
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2019-09-30T15:53:37Z |
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Artículo de revista |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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info:eu-repo/semantics/article |
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Text |
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0123-4226 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/773 |
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305291 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/773 |
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spa |
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spa |
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Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 14, No. 2 (Jul.-Dic. 2011). -- páginas 33-42 |
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Agricultura |
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Derechos Reservados - Universidad de Ciencias Aplicadas y Ambientales |
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Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2011 |
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Guerra, Germán AndrésBetancourth, Carlos ArturoSalazar, Claudia Elizabeth2019-09-30T15:53:37Z2019-09-30T15:53:37Z2011-070123-4226https://revistas.udca.edu.co/index.php/ruadc/article/view/773305291The yellowing pea caused by Fusarium oxysporum is the most problem in the crop and its damage to bring economic losses. This research was carried out in the laboratory and greenhouse of the University of Nariño, located at 2486 masl to an average temperature of 13ºC., in order to evaluate the antagonism of P. fluorescent facing F. oxysporum on pea Pisum sativum L. The isolation of F. oxysporum affected tissue was made from roots and stems of pea plants, which were multiplied and purified on PDA, strain selection was based on the prevalence in all zones and the speed in their growth, obtaining the strain fTR. The isolation of P. fluorescent was made from roots and stems of healthy pea plants that were in focus with yellowing, which were purified and multiplied in Selective Cetrimide Agar, obtaining strains PPC, PPO and PPL; after biochemical tests. The tests were made in dual in medium Nutrient Agar, in which the strain showed higher PPC antagonism to control the growth of F. oxysporum under In vitro conditions in the laboratory. In greenhouse tests the incidence rose from 90.31% to 6.55% with the bacteria compared with the control, showing the efficiency of the antagonistic strain P. fluorescens PPC in the control of F. oxysporum under controlled conditions.Uno de los principales problemas fitopatológicos que afecta el cultivo de arveja es el amarillamiento causado por el hongo Fusarium oxysporum, generando pérdidas considerables en las zonas productoras. Este trabajo, se llevó a cabo en el laboratorio e invernadero de la Universidad de Nariño, situado a una altura de 2.486msnm, a una temperatura promedio de 18ºC, con el fin de evaluar el antagonismo de P. fluorescens frente a F. oxysporum en arveja L. El aislamiento de F. oxysporum, se realizó tomando tejidos afectados de raíces y de tallos de plantas de arveja, los cuales, se sembraron, se purificaron y se multiplicaron en PDA; la selección de la cepa, se efectuó con base en la prevalencia en todas las zonas y por la velocidad en su crecimiento, obteniendo la cepa fTR. El aislamiento de P. fluorescens, se practicó en raíces y en tallos de plantas sanas de arveja, que se encontraban en focos con amarillamiento, que se sembraron y se multiplicaron en agar Cetrimide, obteniendo las cepas PPC, PPO y PPL, después de haber realizado pruebas morfológicas y bioquímicas. Para evaluar el antagonismo, las pruebas duales se produjeron en medio Agar Nutriente, donde la cepa PPC presentó mayor control del crecimiento de F. oxysporum bajo condiciones in vitro. En las pruebas de invernadero, la incidencia de la enfermedad contrastó de un 90,31 a 6,55% con la bacteria, con respecto al testigo, mostrando la eficiencia antagónica de la cepa PPC de P. fluorescens en el control de F. oxysporum, bajo condiciones controladas.Incluye referencias bibliográficasapplication/pdfspaBogotá : Universidad de Ciencias Aplicadas y Ambientales, 2011Revista UDCA : Actualidad & Divulgación Científica (Bogotá). -- Vol. 14, No. 2 (Jul.-Dic. 2011). -- páginas 33-42AgriculturaDerechos Reservados - Universidad de Ciencias Aplicadas y Ambientaleshttps://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_abf2Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L.Antagonismo de Pseudomonas fluorescens Migula frente a Fusarium oxysporum fsp. pisi Schtdl en arveja Pisum sativum L.Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTexthttp://purl.org/redcol/resource_type/ARThttp://purl.org/coar/version/c_970fb48d4fbd8a85Control biológicoRizobacteriaIncidenciaAmarillamientoPseudomonas fluorescensFusarium oxysporum f. sp. pisiPisum sativumControl biológicoPublicationORIGINAL773-Texto del artículo-2364-1-10-20180903.pdf773-Texto del artículo-2364-1-10-20180903.pdfapplication/pdf956588https://repository.udca.edu.co/bitstreams/5f41597b-cf40-4fa5-a2b7-80fb5c36802b/download2039669658e28b81a5599043a77922c8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-814775https://repository.udca.edu.co/bitstreams/99ec4bbb-7339-4e69-8a66-70b867dd0569/downloadf661acf14bedbf9f5d13897a0387e751MD52TEXT773-Texto del artículo-2364-1-10-20180903.pdf.txt773-Texto del artículo-2364-1-10-20180903.pdf.txtExtracted 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 nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

 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