Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción

Passion fruit is a tropical fruit that is usually cultivated to obtain pulp, being the cortex its main not exploited by-product, because of the actual absence of industrial interest. However, with the aim of diversifying the derivates from the production of passion fruit products, it has been demons...

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Autores:
Tarazona Díaz, Martha
Becerra, Nubia
Piedra, Johan
Richard, Beltrán
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
Repositorio:
Repositorio Institucional UDCA
Idioma:
spa
OAI Identifier:
oai:repository.udca.edu.co:11158/3519
Acceso en línea:
https://revistas.udca.edu.co/index.php/ruadc/article/view/1303/1896
Palabra clave:
Passiflora edulis f. flavicarpa
Extractor de Soxhlet
Cáscara del maracuyá
Extracción de carotenoides
Diferencia de color
Passiflora
Carotenoides
Extracción
Rights
License
https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.es
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dc.title.spa.fl_str_mv Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción
dc.title.alternative.spa.fl_str_mv Obtaining a dye from passion fruit cortex with the use of conventional extraction techniques
title Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción
spellingShingle Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción
Passiflora edulis f. flavicarpa
Extractor de Soxhlet
Cáscara del maracuyá
Extracción de carotenoides
Diferencia de color
Passiflora
Carotenoides
Extracción
title_short Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción
title_full Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción
title_fullStr Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción
title_full_unstemmed Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción
title_sort Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción
dc.creator.fl_str_mv Tarazona Díaz, Martha
Becerra, Nubia
Piedra, Johan
Richard, Beltrán
dc.contributor.author.spa.fl_str_mv Tarazona Díaz, Martha
Becerra, Nubia
Piedra, Johan
Richard, Beltrán
dc.subject.proposal.spa.fl_str_mv Passiflora edulis f. flavicarpa
Extractor de Soxhlet
Cáscara del maracuyá
Extracción de carotenoides
Diferencia de color
topic Passiflora edulis f. flavicarpa
Extractor de Soxhlet
Cáscara del maracuyá
Extracción de carotenoides
Diferencia de color
Passiflora
Carotenoides
Extracción
dc.subject.agrovoc.spa.fl_str_mv Passiflora
Carotenoides
Extracción
description Passion fruit is a tropical fruit that is usually cultivated to obtain pulp, being the cortex its main not exploited by-product, because of the actual absence of industrial interest. However, with the aim of diversifying the derivates from the production of passion fruit products, it has been demonstrated that the cortex contains carotenoids, such as β-carotene and lutein; which could be used in food due to its antioxidant capacity, or even like a food colorant. Therefore, the objective of this work was to obtain a carotenoidrich extract from its cortex; for this, three techniques of extraction with ethanol were evaluated, by immersion, thermostatic bath and Soxhlet. Being the last technique that one with which the best yield was obtained. With the highest yield technique, the following factors were evaluated: ethanol concentration on 80% and 90% v/v, solventraw material ratio on 40:1 and 50:1 and time on 90 and 150min. The best yield was obtaining to 90% ethanol, 50mL solvent/g raw material and 150min, for an extraction yield of 2208.53µg β-caroten/100 g sample. Finally, the color difference between the optimal extract and a tartrazine solution was determined, evidencing a color difference of 3.07cieLAB units; this shows that the passion fruit leaching product has the potential to be used as a food additive, replacing synthetic dyes such as tartrazine.
publishDate 2020
dc.date.accessioned.spa.fl_str_mv 2020-08-19T22:25:01Z
dc.date.available.spa.fl_str_mv 2020-08-19T22:25:01Z
dc.date.issued.spa.fl_str_mv 2020-01
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.citation.spa.fl_str_mv Tarazona-Díaz, M.; Becerra, N.; Piedra, J.; Beltrán, R. 2020. Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción. Rev. U.D.C.A Act. & Div. Cient. 23(1):e1303. http://doi.org/10.31910/rudca.v23. n1.2020.1303
dc.identifier.issn.spa.fl_str_mv 0123-4226
dc.identifier.uri.spa.fl_str_mv https://revistas.udca.edu.co/index.php/ruadc/article/view/1303/1896
dc.identifier.doi.spa.fl_str_mv 10.31910/rudca.v23.n1.2020.1303
identifier_str_mv Tarazona-Díaz, M.; Becerra, N.; Piedra, J.; Beltrán, R. 2020. Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción. Rev. U.D.C.A Act. & Div. Cient. 23(1):e1303. http://doi.org/10.31910/rudca.v23. n1.2020.1303
0123-4226
10.31910/rudca.v23.n1.2020.1303
url https://revistas.udca.edu.co/index.php/ruadc/article/view/1303/1896
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.ispartofseries.spa.fl_str_mv Revista U.D.C.A Actualidad & Divulgación Científica (Bogotá);Vol. 23, No. 1, (Ene.-Jun., 2020). -- páginas 1 -10
dc.relation.indexed.spa.fl_str_mv Agricultura
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dc.publisher.spa.fl_str_mv Bogotá : Universidad de Ciencias Aplicadas y Ambientales, 2020
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spelling Tarazona Díaz, MarthaBecerra, NubiaPiedra, JohanRichard, Beltrán2020-08-19T22:25:01Z2020-08-19T22:25:01Z2020-01Tarazona-Díaz, M.; Becerra, N.; Piedra, J.; Beltrán, R. 2020. Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracción. Rev. U.D.C.A Act. & Div. Cient. 23(1):e1303. http://doi.org/10.31910/rudca.v23. n1.2020.13030123-4226https://revistas.udca.edu.co/index.php/ruadc/article/view/1303/189610.31910/rudca.v23.n1.2020.1303Passion fruit is a tropical fruit that is usually cultivated to obtain pulp, being the cortex its main not exploited by-product, because of the actual absence of industrial interest. However, with the aim of diversifying the derivates from the production of passion fruit products, it has been demonstrated that the cortex contains carotenoids, such as β-carotene and lutein; which could be used in food due to its antioxidant capacity, or even like a food colorant. Therefore, the objective of this work was to obtain a carotenoidrich extract from its cortex; for this, three techniques of extraction with ethanol were evaluated, by immersion, thermostatic bath and Soxhlet. Being the last technique that one with which the best yield was obtained. With the highest yield technique, the following factors were evaluated: ethanol concentration on 80% and 90% v/v, solventraw material ratio on 40:1 and 50:1 and time on 90 and 150min. The best yield was obtaining to 90% ethanol, 50mL solvent/g raw material and 150min, for an extraction yield of 2208.53µg β-caroten/100 g sample. Finally, the color difference between the optimal extract and a tartrazine solution was determined, evidencing a color difference of 3.07cieLAB units; this shows that the passion fruit leaching product has the potential to be used as a food additive, replacing synthetic dyes such as tartrazine.El maracuyá es un fruto tropical que se cultiva primordialmente para la obtención de pulpa, siendo la corteza su principal subproducto que carece, en la actualidad, de interés industrial; sin embargo, con el fi n de diversifi car los derivados provenientes de la producción de maracuyá, se ha demostrado que la corteza contiene carotenoides, dentro de los que se destaca el β-caroteno y la luteína, que podrían ser utilizados en alimentos, debido a su poder antioxidante e, incluso, como colorante natural alternativo. Por lo anterior, el objetivo de este trabajo fue obtener un extracto rico en carotenoides a partir de su corteza; para ello, se evaluó la extracción con etanol, mediante tres técnicas: inmersión, baño termostático y soxhlet. El mayor rendimiento, se obtuvo con el método soxhlet; para esta técnica, se estudió el efecto de la concentración de etanol (entre 80% y 90% v/v), la proporción solvente-materia prima (con relaciones entre 40:1 y 50:1) y el tiempo (defi nido entre 90 y 150 minutos). Los resultados, se analizaron mediante modelo de superfi cie de respuesta, obteniendo el mayor rendimiento con etanol al 90%, utilizando 50mL/g corteza y 150 minutos de operación. A estas condiciones, el rendimiento de extracción fue de 2208,53µg β-caroteno/100g muestra. Por último, se determinó la diferencia de color entre el extracto óptimo y una solución de tartrazina, evidenciando una diferencia de color de 3,07 unidades cieLAB, lo cual, muestra que el producto de lixiviación de la corteza de maracuyá tiene potencial para su uso como aditivo alimentario, reemplazando colorantes sintéticos, como la tartrazina.application/pdfspaBogotá : Universidad de Ciencias Aplicadas y Ambientales, 2020Revista U.D.C.A Actualidad & Divulgación Científica (Bogotá);Vol. 23, No. 1, (Ene.-Jun., 2020). -- páginas 1 -10Agriculturahttps://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.esAtribución-NoComercial-CompartirIgualhttp://purl.org/coar/access_right/c_abf2https://revistas.udca.edu.co/index.php/ruadc/article/view/1303/1896https://revistas.udca.edu.co/index.php/ruadc/article/view/1303/1896Obtención de un colorante a partir de corteza de maracuyá con el uso de técnicas convencionales de extracciónObtaining a dye from passion fruit cortex with the use of conventional extraction techniquesArtículo de revistainfo:eu-repo/semantics/articleTexthttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1Passiflora edulis f. flavicarpaExtractor de SoxhletCáscara del maracuyáExtracción de carotenoidesDiferencia de colorPassifloraCarotenoidesExtracciónPublicationORIGINALdocument (5).pdfdocument (5).pdfapplication/pdf1872466https://repository.udca.edu.co/bitstreams/9ca8195b-1001-4c4e-a367-48d24d8f1c39/download8400192f758da45e894452316190f210MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-814775https://repository.udca.edu.co/bitstreams/dec44f9c-4ac9-4029-8969-84d3c7b656a1/downloadf661acf14bedbf9f5d13897a0387e751MD52TEXTdocument (5).pdf.txtdocument (5).pdf.txtExtracted texttext/plain37570https://repository.udca.edu.co/bitstreams/b2f5720d-8348-4396-b007-5d0b363b564b/downloaded99a9c212561578e1e77d4f89fbf1d0MD53THUMBNAILdocument (5).pdf.jpgdocument (5).pdf.jpgGenerated 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en las Obras Colectivas;
b.	Distribuir copias o fonogramas de las Obras, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública, incluyéndolas como incorporadas en Obras Colectivas, según corresponda;
c.	Distribuir copias de las Obras Derivadas que se generen, exhibirlas públicamente, ejecutarlas públicamente y/o ponerlas a disposición pública.
Los derechos mencionados anteriormente pueden ser ejercidos en todos los medios y formatos, actualmente conocidos o que se inventen en el futuro. Los derechos antes mencionados incluyen el derecho a realizar dichas modificaciones en la medida que sean técnicamente necesarias para ejercer los derechos en otro medio o formatos, pero de otra manera usted no está autorizado para realizar obras derivadas.Todos los derechos no otorgados expresamente por el Licenciante quedan por este medio reservados, incluyendo pero sin limitarse a aquellos que se mencionan en las secciones 4(d) y 4(e).
4. Restricciones.
La licencia otorgada en la anterior Sección 3 está expresamente sujeta y limitada por las siguientes restricciones:
a.	Usted puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra sólo bajo las condiciones de esta Licencia, y Usted debe incluir una copia de esta licencia o del Identificador Universal de Recursos de la misma con cada copia de la Obra que distribuya, exhiba públicamente, ejecute públicamente o ponga a disposición pública. No es posible ofrecer o imponer ninguna condición sobre la Obra que altere o limite las condiciones de esta Licencia o el ejercicio de los derechos de los destinatarios otorgados en este documento. No es posible sublicenciar la Obra. Usted debe mantener intactos todos los avisos que hagan referencia a esta Licencia y a la cláusula de limitación de garantías. Usted no puede distribuir, exhibir públicamente, ejecutar públicamente, o poner a disposición pública la Obra con alguna medida tecnológica que controle el acceso o la utilización de ella de una forma que sea inconsistente con las condiciones de esta Licencia. Lo anterior se aplica a la Obra incorporada a una Obra Colectiva, pero esto no exige que la Obra Colectiva aparte de la obra misma quede sujeta a las condiciones de esta Licencia. Si Usted crea una Obra Colectiva, previo aviso de cualquier Licenciante debe, en la medida de lo posible, eliminar de la Obra Colectiva cualquier referencia a dicho Licenciante o al Autor Original, según lo solicitado por el Licenciante y conforme lo exige la cláusula 4(c).
b.	Usted no puede ejercer ninguno de los derechos que le han sido otorgados en la Sección 3 precedente de modo que estén principalmente destinados o directamente dirigidos a conseguir un provecho comercial o una compensación monetaria privada. El intercambio de la Obra por otras obras protegidas por derechos de autor, ya sea a través de un sistema para compartir archivos digitales (digital file-sharing) o de cualquier otra manera no será considerado como estar destinado principalmente o dirigido directamente a conseguir un provecho comercial o una compensación monetaria privada, siempre que no se realice un pago mediante una compensación monetaria en relación con el intercambio de obras protegidas por el derecho de autor.
c.	Si usted distribuye, exhibe públicamente, ejecuta públicamente o ejecuta públicamente en forma digital la Obra o cualquier Obra Derivada u Obra Colectiva, Usted debe mantener intacta toda la información de derecho de autor de la Obra y proporcionar, de forma razonable según el medio o manera que Usted esté utilizando: (i) el nombre del Autor Original si está provisto (o seudónimo, si fuere aplicable), y/o (ii) el nombre de la parte o las partes que el Autor Original y/o el Licenciante hubieren designado para la atribución (v.g., un instituto patrocinador, editorial, publicación) en la información de los derechos de autor del Licenciante, términos de servicios o de otras formas razonables; el título de la Obra si está provisto; en la medida de lo razonablemente factible y, si está provisto, el Identificador Uniforme de Recursos (Uniform Resource Identifier) que el Licenciante especifica para ser asociado con la Obra, salvo que tal URI no se refiera a la nota sobre los derechos de autor o a la información sobre el licenciamiento de la Obra; y en el caso de una Obra Derivada, atribuir el crédito identificando el uso de la Obra en la Obra Derivada (v.g., "Traducción Francesa de la Obra del Autor Original," o "Guión Cinematográfico basado en la Obra original del Autor Original"). Tal crédito puede ser implementado de cualquier forma razonable; en el caso, sin embargo, de Obras Derivadas u Obras Colectivas, tal crédito aparecerá, como mínimo, donde aparece el crédito de cualquier otro autor comparable y de una manera, al menos, tan destacada como el crédito de otro autor comparable.
d.	Para evitar toda confusión, el Licenciante aclara que, cuando la obra es una composición musical:
i.	Regalías por interpretación y ejecución bajo licencias generales. El Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública o la ejecución pública digital de la obra y de recolectar, sea individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), las regalías por la ejecución pública o por la ejecución pública digital de la obra (por ejemplo Webcast) licenciada bajo licencias generales, si la interpretación o ejecución de la obra está primordialmente orientada por o dirigida a la obtención de una ventaja comercial o una compensación monetaria privada.
ii.	Regalías por Fonogramas. El Licenciante se reserva el derecho exclusivo de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, SAYCO), una agencia de derechos musicales o algún agente designado, las regalías por cualquier fonograma que Usted cree a partir de la obra (“versión cover”) y distribuya, en los términos del régimen de derechos de autor, si la creación o distribución de esa versión cover está primordialmente destinada o dirigida a obtener una ventaja comercial o una compensación monetaria privada.
e.	Gestión de Derechos de Autor sobre Interpretaciones y Ejecuciones Digitales (WebCasting). Para evitar toda confusión, el Licenciante aclara que, cuando la obra sea un fonograma, el Licenciante se reserva el derecho exclusivo de autorizar la ejecución pública digital de la obra (por ejemplo, webcast) y de recolectar, individualmente o a través de una sociedad de gestión colectiva de derechos de autor y derechos conexos (por ejemplo, Acinpro), las regalías por la ejecución pública digital de la obra (por ejemplo, webcast), sujeta a las disposiciones aplicables del régimen de Derecho de Autor, si esta ejecución pública digital está primordialmente dirigida a obtener una ventaja comercial o una compensación monetaria privada.
5. Representaciones, Garantías y Limitaciones de Responsabilidad.
A MENOS QUE LAS PARTES LO ACORDARAN DE OTRA FORMA POR ESCRITO, EL LICENCIANTE OFRECE LA OBRA (EN EL ESTADO EN EL QUE SE ENCUENTRA) “TAL CUAL”, SIN BRINDAR GARANTÍAS DE CLASE ALGUNA RESPECTO DE LA OBRA, YA SEA EXPRESA, IMPLÍCITA, LEGAL O CUALQUIERA OTRA, INCLUYENDO, SIN LIMITARSE A ELLAS, GARANTÍAS DE TITULARIDAD, COMERCIABILIDAD, ADAPTABILIDAD O ADECUACIÓN A PROPÓSITO DETERMINADO, AUSENCIA DE INFRACCIÓN, DE AUSENCIA DE DEFECTOS LATENTES O DE OTRO TIPO, O LA PRESENCIA O AUSENCIA DE ERRORES, SEAN O NO DESCUBRIBLES (PUEDAN O NO SER ESTOS DESCUBIERTOS). ALGUNAS JURISDICCIONES NO PERMITEN LA EXCLUSIÓN DE GARANTÍAS IMPLÍCITAS, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
6. Limitación de responsabilidad.
A MENOS QUE LO EXIJA EXPRESAMENTE LA LEY APLICABLE, EL LICENCIANTE NO SERÁ RESPONSABLE ANTE USTED POR DAÑO ALGUNO, SEA POR RESPONSABILIDAD EXTRACONTRACTUAL, PRECONTRACTUAL O CONTRACTUAL, OBJETIVA O SUBJETIVA, SE TRATE DE DAÑOS MORALES O PATRIMONIALES, DIRECTOS O INDIRECTOS, PREVISTOS O IMPREVISTOS PRODUCIDOS POR EL USO DE ESTA LICENCIA O DE LA OBRA, AUN CUANDO EL LICENCIANTE HAYA SIDO ADVERTIDO DE LA POSIBILIDAD DE DICHOS DAÑOS. ALGUNAS LEYES NO PERMITEN LA EXCLUSIÓN DE CIERTA RESPONSABILIDAD, EN CUYO CASO ESTA EXCLUSIÓN PUEDE NO APLICARSE A USTED.
7. Término.
a.	Esta Licencia y los derechos otorgados en virtud de ella terminarán automáticamente si Usted infringe alguna condición establecida en ella. Sin embargo, los individuos o entidades que han recibido Obras Derivadas o Colectivas de Usted de conformidad con esta Licencia, no verán terminadas sus licencias, siempre que estos individuos o entidades sigan cumpliendo íntegramente las condiciones de estas licencias. Las Secciones 1, 2, 5, 6, 7, y 8 subsistirán a cualquier terminación de esta Licencia.
b.	Sujeta a las condiciones y términos anteriores, la licencia otorgada aquí es perpetua (durante el período de vigencia de los derechos de autor de la obra). No obstante lo anterior, el Licenciante se reserva el derecho a publicar y/o estrenar la Obra bajo condiciones de licencia diferentes o a dejar de distribuirla en los términos de esta Licencia en cualquier momento; en el entendido, sin embargo, que esa elección no servirá para revocar esta licencia o que deba ser otorgada , bajo los términos de esta licencia), y esta licencia continuará en pleno vigor y efecto a menos que sea terminada como se expresa atrás. La Licencia revocada continuará siendo plenamente vigente y efectiva si no se le da término en las condiciones indicadas anteriormente.
8. Varios.
a.	Cada vez que Usted distribuya o ponga a disposición pública la Obra o una Obra Colectiva, el Licenciante ofrecerá al destinatario una licencia en los mismos términos y condiciones que la licencia otorgada a Usted bajo esta Licencia.
b.	Si alguna disposición de esta Licencia resulta invalidada o no exigible, según la legislación vigente, esto no afectará ni la validez ni la aplicabilidad del resto de condiciones de esta Licencia y, sin acción adicional por parte de los sujetos de este acuerdo, aquélla se entenderá reformada lo mínimo necesario para hacer que dicha disposición sea válida y exigible.
c.	Ningún término o disposición de esta Licencia se estimará renunciada y ninguna violación de ella será consentida a menos que esa renuncia o consentimiento sea otorgado por escrito y firmado por la parte que renuncie o consienta.
d.	Esta Licencia refleja el acuerdo pleno entre las partes respecto a la Obra aquí licenciada. No hay arreglos, acuerdos o declaraciones respecto a la Obra que no estén especificados en este documento. El Licenciante no se verá limitado por ninguna disposición adicional que pueda surgir en alguna comunicación emanada de Usted. Esta Licencia no puede ser modificada sin el consentimiento mutuo por escrito del Licenciante y Usted.

