Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]

Background: In a previous work we showed the feasibility of an interferon gamma release assay (IGRA) for detecting latent infection by Histoplasma capsulatum. While in that proof-of-concept study we used crude fungal extracts as antigens, the newest IGRAs developed for other infections are based on...

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Fecha de publicación:: 2019

Institución:: Universidad de Medellín

Repositorio:: Repositorio UDEM

Idioma:: eng
spa

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network_acronym_str	REPOUDEM2
network_name_str	Repositorio UDEM
repository_id_str
dc.title.none.fl_str_mv	Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]
title	Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]
spellingShingle	Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum] Histoplasma capsulatum IGRA Immunogenic proteins Molecularly defined antigens T-cell epitopes alcohol dehydrogenase ankyrin repeat protein beta lactamase chaperonin 60 DUF636 domain containing protein epitope fungus antigen M antigen proteoglycan synthetic peptide unclassified drug allele amino acid sequence antigen detection antigenic variation Article controlled study Histoplasma capsulatum human immunogenicity interferon gamma release assay nonhuman orthology pathogenicity protein analysis sensitivity and specificity volunteer
title_short	Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]
title_full	Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]
title_fullStr	Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]
title_full_unstemmed	Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]
title_sort	Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]
dc.subject.none.fl_str_mv	Histoplasma capsulatum IGRA Immunogenic proteins Molecularly defined antigens T-cell epitopes alcohol dehydrogenase ankyrin repeat protein beta lactamase chaperonin 60 DUF636 domain containing protein epitope fungus antigen M antigen proteoglycan synthetic peptide unclassified drug allele amino acid sequence antigen detection antigenic variation Article controlled study Histoplasma capsulatum human immunogenicity interferon gamma release assay nonhuman orthology pathogenicity protein analysis sensitivity and specificity volunteer
topic	Histoplasma capsulatum IGRA Immunogenic proteins Molecularly defined antigens T-cell epitopes alcohol dehydrogenase ankyrin repeat protein beta lactamase chaperonin 60 DUF636 domain containing protein epitope fungus antigen M antigen proteoglycan synthetic peptide unclassified drug allele amino acid sequence antigen detection antigenic variation Article controlled study Histoplasma capsulatum human immunogenicity interferon gamma release assay nonhuman orthology pathogenicity protein analysis sensitivity and specificity volunteer
description	Background: In a previous work we showed the feasibility of an interferon gamma release assay (IGRA) for detecting latent infection by Histoplasma capsulatum. While in that proof-of-concept study we used crude fungal extracts as antigens, the newest IGRAs developed for other infections are based on molecularly defined antigens, mostly on mixtures of immunogenic peptides. Aims: To identify proteins in H. capsulatum that might serve as molecularly defined antigens for an IGRA test. Methods: We surveyed the literature looking for known H. capsulatum-immunogenic proteins and assayed two of them as antigens in an IGRA test, in a study that involved 80 volunteers. Furthermore, we used several bioinformatics tools to identify specific H. capsulatum proteins and to analyze possible strategies for the design of H. capsulatum-specific immunogenic peptides. Results: Seven H. capsulatum-immunogenic proteins were retrieved from the literature. IGRA tests using either the heat shock protein 60 or the M antigen showed high sensitivities but low specificities, most likely due to the high sequence similarity with the corresponding orthologs in other pathogenic microorganisms. We identified around 2000 H. capsulatum-specific proteins, most of which remain unannotated. Class II T-cell epitope predictions for a small number of these proteins showed a great variability among different alleles, prompting for a brute force approach for peptide design. Conclusions: The H. capsulatum genome encodes a large number of distinctive proteins, which represent a valuable source of potential specific antigens for an IGRA test. Among them, the Cfp4 protein stands out as a very attractive candidate. © 2019 Asociación Española de Micología
publishDate	2019
dc.date.accessioned.none.fl_str_mv	2020-04-29T14:54:07Z
dc.date.available.none.fl_str_mv	2020-04-29T14:54:07Z
dc.date.none.fl_str_mv	2019
dc.type.eng.fl_str_mv	Article
dc.type.coarversion.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_6501 http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/article
dc.identifier.issn.none.fl_str_mv	11301406
dc.identifier.uri.none.fl_str_mv	http://hdl.handle.net/11407/5812
dc.identifier.doi.none.fl_str_mv	10.1016/j.riam.2019.06.002
identifier_str_mv	11301406 10.1016/j.riam.2019.06.002
url	http://hdl.handle.net/11407/5812
dc.language.iso.none.fl_str_mv	eng spa
language	eng spa
dc.relation.isversionof.none.fl_str_mv	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075592310&doi=10.1016%2fj.riam.2019.06.002&partnerID=40&md5=af0fd57be13f4e5f724549fc8c674fa4
dc.relation.citationvolume.none.fl_str_mv	36
dc.relation.citationissue.none.fl_str_mv	4
dc.relation.citationstartpage.none.fl_str_mv	186
dc.relation.citationendpage.none.fl_str_mv	191
dc.relation.references.none.fl_str_mv	Aagaard, C., Brock, I., Olsen, A., Ottenhoff, T.H.M., Weldingh, K., Andersen, P., Mapping immune reactivity toward Rv2653 and Rv2654: two novel low-molecular-mass antigens found specifically in the Mycobacterium tuberculosis complex (2004) J Infect Dis, 189, pp. 812-819 Adenis, A., Aznar, C., Couppié, P., Histoplasmosis in HIV-infected patients: a review of new developments and remaining gaps (2014) Curr Trop Med Rep, 1, pp. 119-128 Albuquerque, P.C., Nakayasu, E.S., Rodrigues, M.L., Frases, S., Casadevall, A., Zancope-Oliveira, R.M., Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes (2008) Cell Microbiol, 10, pp. 1695-1710 Allendoerfer, R., Deepe, G.S., Intrapulmonary response to Histoplasma capsulatum in gamma interferon knockout mice (1997) Infect Immun, 65, pp. 2564-2569 Barcellini, L., Borroni, E., Brown, J., Brunetti, E., Codecasa, L., Cugnata, F., First independent evaluation of QuantiFERON-TB Plus performance (2016) Eur Respir J, 47, pp. 1587-1590 Bendtsen, J.D., Jensen, L.J., Blom, N., Von Heijne, G., Brunak, S., Feature-based prediction of non-classical and leaderless protein secretion (2004) Protein Eng Des Sel, 17, pp. 349-356 Chapey, E., Wallon, M., Debize, G., Rabilloud, M., Peyron, F., Diagnosis of congenital toxoplasmosis by using a whole-blood gamma interferon release assay (2010) J Clin Microbiol, 48, pp. 41-45 Dammermann, W., Bentzien, F., Stiel, E.M., Kühne, C., Ullrich, S., zur Wiesch, J.S., Development of a novel IGRA assay to test T cell responsiveness to HBV antigens in whole blood of chronic hepatitis B patients (2015) J Transl Med, 13, p. 157 Deepe, G.S., Histoplasma capsulatum (histoplasmosis) (2014) Mandell, Douglas, and Bennett's principles and practice of infectious diseases, pp. 2949-2962 Dominguez, M.R., Silveira, E.L., de Vasconcelos, J.R., de Alencar, B.C., Machado, A.V., Bruna-Romero, O., Subdominant/cryptic CD8 T cell epitopes contribute to resistance against experimental infection with a human protozoan parasite (2011) PLoS ONE, 6, pp. 1-12 Emanuelsson, O., Nielsen, H., Brunak, S., von Heijne, G., Predicting subcellular localization of proteins based on their N-terminal amino acid sequence (2000) J Mol Biol, 300, pp. 1005-1016 Gidwani, K., Jones, S., Kumar, R., Boelaert, M., Sundar, S., Interferon-Gamma release assay (modified quantiFERON) as a potential marker of infection for Leishmania donovani, a proof of concept study (2011) PLoS Negl Trop Dis, 5, p. e1042 Giulieri, S., Manuel, O., QuantiFERON®-CMV assay for the assessment of cytomegalovirus cell-mediated immunity (2011) Expert Rev Mol Diagn, 11, pp. 17-25 Guimarães, A.J., Hamilton, A.J., de M Guedes, H.L., Nosanchuk, J.D., Zancopé-Oliveira, R.M., Biological function and molecular mapping of M antigen in yeast phase of Histoplasma capsulatum (2008) PLoS ONE, 3, p. e3449 Guimarães, A.J., Frases, S., Gomez, F.J., Zancopé-Oliveira, R.M., Nosanchuk, J.D., Monoclonal antibodies to heat shock protein 60 alter the pathogenesis of Histoplasma capsulatum (2009) Infect Immun, 77, pp. 1357-1367 Hober, D., Benyoucef, S., Chehadeh, W., Hober, D., Benyoucef, S., Chehadeh, W., Ex vivo interferon-gamma response to human immunodeficiency virus-1 derived peptides in human immunodeficiency virus-1 infected patients (2000) Scand J Immunol, 51, pp. 429-433 Holbrook, E.D., Kemski, M.M., Richer, S.M., Wheat, L.J., Rappleye, C.A., Glycosylation and immunoreactivity of the Histoplasma capsulatum Cfp4 yeast-phase exoantigen (2014) Infect Immun, 82, pp. 4414-4425 Horwath, M.C., Fecher, R.A., Deepe, G.S., Histoplasma capsulatum, lung infection and immunity (2015) Future Microbiol, 10, pp. 967-975 Jensen, K.K., Andreatta, M., Marcatili, P., Buus, S., Greenbaum, J.A., Yan, Z., Improved methods for predicting peptide binding affinity to MHC class II molecules (2018) Immunology, 154, pp. 394-406 Krogh, A., Larsson, B., Von Heijne, G., Sonnhammer, E.L.L., Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes (2001) J Mol Biol, 305, pp. 567-580 Li, L., Stoeckert, C.J., Roos, D.S., OrthoMCL: identification of ortholog groups for eukaryotic genomes (2003) Genome Res, 13, pp. 2178-2189 Mazurek, G.H., Villarino, M.E., Guidelines for using the QuantiFERON-TB test for diagnosing latent Mycobacterium tuberculosis infection. Centers for Disease Control and Prevention (2003) MMWR Recomm Rep, 52, pp. 15-18 Nel, J.S., Bartelt, L.A., van Duin, D., Lachiewicz, A.M., Endemic mycoses in solid organ transplant recipients (2018) Infect Dis Clin N Am, 32, pp. 667-685 Rubio-Carrasquilla, M., Santa, C.D., Rendón, J.P., Botero-Garcés, J., Moreno, E., Cano, L.E., An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: a proof of concept study (2019) Med Mycol, 57, pp. 724-732 Sifuentes-Osornio, J., Corzo-León, D.E., Ponce-De-León, L.A., Epidemiology of invasive fungal infections in Latin America (2012) Curr Fungal Infect Rep, 6, pp. 23-34 Smith, J.G., Liu, X., Kaufhold, R.M., Clair, J., Caulfield, M.J., Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune responses to varicella-zoster virus (2001) Clin Diagn Lab Immunol, 8, pp. 871-879 Turgay, N., Balcioglu, I.C., Toz, S.O., Ozbel, Y., Jones, S.L., Short report: Quantiferon-Leishmania as an epidemiological tool for evaluating the exposure to Leishmania infection (2010) Am J Trop Med Hyg, 83, pp. 822-824 Vergidis, P., Avery, R.K., Wheat, L.J., Dotson, J.L., Assi, M.A., Antoun, S.A., Histoplasmosis complicating tumor necrosis factor-? blocker therapy: a retrospective analysis of 98 cases (2015) Clin Infect Dis, 61, pp. 409-417 Weir, R.E., Morgan, A.R., Britton, W.J., Butlin, C.R., Dockrell, H.M., Development of a whole blood assay to measure T cell responses to leprosy: a new tool for immuno-epidemiological field studies of leprosy immunity (1994) J Immunol Methods, 176, pp. 93-101
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_16ec
rights_invalid_str_mv	http://purl.org/coar/access_right/c_16ec
dc.publisher.none.fl_str_mv	Asociacion Espanola de Micologia
dc.publisher.program.none.fl_str_mv	Facultad de Ciencias Básicas
dc.publisher.faculty.none.fl_str_mv	Facultad de Ciencias Básicas
publisher.none.fl_str_mv	Asociacion Espanola de Micologia
dc.source.none.fl_str_mv	Revista Iberoamericana de Micologia
institution	Universidad de Medellín
repository.name.fl_str_mv	Repositorio Institucional Universidad de Medellin
repository.mail.fl_str_mv	repositorio@udem.edu.co
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spelling	20192020-04-29T14:54:07Z2020-04-29T14:54:07Z11301406http://hdl.handle.net/11407/581210.1016/j.riam.2019.06.002Background: In a previous work we showed the feasibility of an interferon gamma release assay (IGRA) for detecting latent infection by Histoplasma capsulatum. While in that proof-of-concept study we used crude fungal extracts as antigens, the newest IGRAs developed for other infections are based on molecularly defined antigens, mostly on mixtures of immunogenic peptides. Aims: To identify proteins in H. capsulatum that might serve as molecularly defined antigens for an IGRA test. Methods: We surveyed the literature looking for known H. capsulatum-immunogenic proteins and assayed two of them as antigens in an IGRA test, in a study that involved 80 volunteers. Furthermore, we used several bioinformatics tools to identify specific H. capsulatum proteins and to analyze possible strategies for the design of H. capsulatum-specific immunogenic peptides. Results: Seven H. capsulatum-immunogenic proteins were retrieved from the literature. IGRA tests using either the heat shock protein 60 or the M antigen showed high sensitivities but low specificities, most likely due to the high sequence similarity with the corresponding orthologs in other pathogenic microorganisms. We identified around 2000 H. capsulatum-specific proteins, most of which remain unannotated. Class II T-cell epitope predictions for a small number of these proteins showed a great variability among different alleles, prompting for a brute force approach for peptide design. Conclusions: The H. capsulatum genome encodes a large number of distinctive proteins, which represent a valuable source of potential specific antigens for an IGRA test. Among them, the Cfp4 protein stands out as a very attractive candidate. © 2019 Asociación Española de MicologíaengspaAsociacion Espanola de MicologiaFacultad de Ciencias BásicasFacultad de Ciencias Básicashttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85075592310&doi=10.1016%2fj.riam.2019.06.002&partnerID=40&md5=af0fd57be13f4e5f724549fc8c674fa4364186191Aagaard, C., Brock, I., Olsen, A., Ottenhoff, T.H.M., Weldingh, K., Andersen, P., Mapping immune reactivity toward Rv2653 and Rv2654: two novel low-molecular-mass antigens found specifically in the Mycobacterium tuberculosis complex (2004) J Infect Dis, 189, pp. 812-819Adenis, A., Aznar, C., Couppié, P., Histoplasmosis in HIV-infected patients: a review of new developments and remaining gaps (2014) Curr Trop Med Rep, 1, pp. 119-128Albuquerque, P.C., Nakayasu, E.S., Rodrigues, M.L., Frases, S., Casadevall, A., Zancope-Oliveira, R.M., Vesicular transport in Histoplasma capsulatum: an effective mechanism for trans-cell wall transfer of proteins and lipids in ascomycetes (2008) Cell Microbiol, 10, pp. 1695-1710Allendoerfer, R., Deepe, G.S., Intrapulmonary response to Histoplasma capsulatum in gamma interferon knockout mice (1997) Infect Immun, 65, pp. 2564-2569Barcellini, L., Borroni, E., Brown, J., Brunetti, E., Codecasa, L., Cugnata, F., First independent evaluation of QuantiFERON-TB Plus performance (2016) Eur Respir J, 47, pp. 1587-1590Bendtsen, J.D., Jensen, L.J., Blom, N., Von Heijne, G., Brunak, S., Feature-based prediction of non-classical and leaderless protein secretion (2004) Protein Eng Des Sel, 17, pp. 349-356Chapey, E., Wallon, M., Debize, G., Rabilloud, M., Peyron, F., Diagnosis of congenital toxoplasmosis by using a whole-blood gamma interferon release assay (2010) J Clin Microbiol, 48, pp. 41-45Dammermann, W., Bentzien, F., Stiel, E.M., Kühne, C., Ullrich, S., zur Wiesch, J.S., Development of a novel IGRA assay to test T cell responsiveness to HBV antigens in whole blood of chronic hepatitis B patients (2015) J Transl Med, 13, p. 157Deepe, G.S., Histoplasma capsulatum (histoplasmosis) (2014) Mandell, Douglas, and Bennett's principles and practice of infectious diseases, pp. 2949-2962Dominguez, M.R., Silveira, E.L., de Vasconcelos, J.R., de Alencar, B.C., Machado, A.V., Bruna-Romero, O., Subdominant/cryptic CD8 T cell epitopes contribute to resistance against experimental infection with a human protozoan parasite (2011) PLoS ONE, 6, pp. 1-12Emanuelsson, O., Nielsen, H., Brunak, S., von Heijne, G., Predicting subcellular localization of proteins based on their N-terminal amino acid sequence (2000) J Mol Biol, 300, pp. 1005-1016Gidwani, K., Jones, S., Kumar, R., Boelaert, M., Sundar, S., Interferon-Gamma release assay (modified quantiFERON) as a potential marker of infection for Leishmania donovani, a proof of concept study (2011) PLoS Negl Trop Dis, 5, p. e1042Giulieri, S., Manuel, O., QuantiFERON®-CMV assay for the assessment of cytomegalovirus cell-mediated immunity (2011) Expert Rev Mol Diagn, 11, pp. 17-25Guimarães, A.J., Hamilton, A.J., de M Guedes, H.L., Nosanchuk, J.D., Zancopé-Oliveira, R.M., Biological function and molecular mapping of M antigen in yeast phase of Histoplasma capsulatum (2008) PLoS ONE, 3, p. e3449Guimarães, A.J., Frases, S., Gomez, F.J., Zancopé-Oliveira, R.M., Nosanchuk, J.D., Monoclonal antibodies to heat shock protein 60 alter the pathogenesis of Histoplasma capsulatum (2009) Infect Immun, 77, pp. 1357-1367Hober, D., Benyoucef, S., Chehadeh, W., Hober, D., Benyoucef, S., Chehadeh, W., Ex vivo interferon-gamma response to human immunodeficiency virus-1 derived peptides in human immunodeficiency virus-1 infected patients (2000) Scand J Immunol, 51, pp. 429-433Holbrook, E.D., Kemski, M.M., Richer, S.M., Wheat, L.J., Rappleye, C.A., Glycosylation and immunoreactivity of the Histoplasma capsulatum Cfp4 yeast-phase exoantigen (2014) Infect Immun, 82, pp. 4414-4425Horwath, M.C., Fecher, R.A., Deepe, G.S., Histoplasma capsulatum, lung infection and immunity (2015) Future Microbiol, 10, pp. 967-975Jensen, K.K., Andreatta, M., Marcatili, P., Buus, S., Greenbaum, J.A., Yan, Z., Improved methods for predicting peptide binding affinity to MHC class II molecules (2018) Immunology, 154, pp. 394-406Krogh, A., Larsson, B., Von Heijne, G., Sonnhammer, E.L.L., Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes (2001) J Mol Biol, 305, pp. 567-580Li, L., Stoeckert, C.J., Roos, D.S., OrthoMCL: identification of ortholog groups for eukaryotic genomes (2003) Genome Res, 13, pp. 2178-2189Mazurek, G.H., Villarino, M.E., Guidelines for using the QuantiFERON-TB test for diagnosing latent Mycobacterium tuberculosis infection. Centers for Disease Control and Prevention (2003) MMWR Recomm Rep, 52, pp. 15-18Nel, J.S., Bartelt, L.A., van Duin, D., Lachiewicz, A.M., Endemic mycoses in solid organ transplant recipients (2018) Infect Dis Clin N Am, 32, pp. 667-685Rubio-Carrasquilla, M., Santa, C.D., Rendón, J.P., Botero-Garcés, J., Moreno, E., Cano, L.E., An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: a proof of concept study (2019) Med Mycol, 57, pp. 724-732Sifuentes-Osornio, J., Corzo-León, D.E., Ponce-De-León, L.A., Epidemiology of invasive fungal infections in Latin America (2012) Curr Fungal Infect Rep, 6, pp. 23-34Smith, J.G., Liu, X., Kaufhold, R.M., Clair, J., Caulfield, M.J., Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune responses to varicella-zoster virus (2001) Clin Diagn Lab Immunol, 8, pp. 871-879Turgay, N., Balcioglu, I.C., Toz, S.O., Ozbel, Y., Jones, S.L., Short report: Quantiferon-Leishmania as an epidemiological tool for evaluating the exposure to Leishmania infection (2010) Am J Trop Med Hyg, 83, pp. 822-824Vergidis, P., Avery, R.K., Wheat, L.J., Dotson, J.L., Assi, M.A., Antoun, S.A., Histoplasmosis complicating tumor necrosis factor-? blocker therapy: a retrospective analysis of 98 cases (2015) Clin Infect Dis, 61, pp. 409-417Weir, R.E., Morgan, A.R., Britton, W.J., Butlin, C.R., Dockrell, H.M., Development of a whole blood assay to measure T cell responses to leprosy: a new tool for immuno-epidemiological field studies of leprosy immunity (1994) J Immunol Methods, 176, pp. 93-101Revista Iberoamericana de MicologiaHistoplasma capsulatumIGRAImmunogenic proteinsMolecularly defined antigensT-cell epitopesalcohol dehydrogenaseankyrin repeat proteinbeta lactamasechaperonin 60DUF636 domain containing proteinepitopefungus antigenM antigenproteoglycansynthetic peptideunclassified drugalleleamino acid sequenceantigen detectionantigenic variationArticlecontrolled studyHistoplasma capsulatumhumanimmunogenicityinterferon gamma release assaynonhumanorthologypathogenicityprotein analysissensitivity and specificityvolunteerIdentifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]Articleinfo:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Rubio-Carrasquilla, M., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia, Instituto de Biología, Universidad de Antioquia, Medellín, Colombia; Ochoa, R., Programa de Estudio y Control de Enfermedades Tropicales PECET, Universidad de Antioquia, Medellín, Colombia; Santa, C., Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia; Guimarães, A.J., Depto de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, RJ, Brazil; Cano, L.E., Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia; Moreno, E., Facultad de Ciencias Básicas, Universidad de Medellín, Medellín, Colombiahttp://purl.org/coar/access_right/c_16ecRubio-Carrasquilla M.Ochoa R.Santa C.Guimarães A.J.Cano L.E.Moreno E.11407/5812oai:repository.udem.edu.co:11407/58122020-12-15 15:05:30.349Repositorio Institucional Universidad de Medellinrepositorio@udem.edu.co

Identifying molecularly defined antigens for a Histoplasma capsulatum-specific interferon gamma release assay [Identificación de antígenos definidos molecularmente para un ensayo de liberación de interferón-gamma específico para Histoplasma capsulatum]

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