TRF1 as a major contributor for telomeres’ shortening in the context of obesity

Obesity is a prevalent multifactorial chronic disorder characterized by metabolic dysregulation. Sustained pro-oxidative mediators trigger harmful consequences that reflect at systemic level and contribute for the establishment of a premature senescent phenotype associated with macromolecular damage...

Full description

Autores:
Grun Kich, Lucas
Nevton Da Rosa, Teixeira
Mengden Von, Lúcia
De Bastiani, Marco Antônio
Parisi Migliorini, Mariana
Calixto Bortolin, Rafael
Lavandoski, Patrícia
Pierdona, Vinícius
Alves Biscaino, Letícia
Moreira Fonseca, José Cláudio
Mottin Corá, Cláudio
Jones, Marcus Herbert
Klamt, Fábio
Vontobel Padoin, Alexandre
Guma Costa R.Rodrigues, Fátima
Barbé Tuana, Florência María
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Corporación Universidad de la Costa
Repositorio:
REDICUC - Repositorio CUC
Idioma:
eng
OAI Identifier:
oai:repositorio.cuc.edu.co:11323/932
Acceso en línea:
http://hdl.handle.net/11323/932
https://repositorio.cuc.edu.co/
Palabra clave:
Aging
Obesity
Oxidative stress
Shelterin complex
Telomere length
TRF1
Rights
openAccess
License
Atribución – No comercial – Compartir igual
id RCUC2_f0535b23d6bd8500859e60a711ce7723
oai_identifier_str oai:repositorio.cuc.edu.co:11323/932
network_acronym_str RCUC2
network_name_str REDICUC - Repositorio CUC
repository_id_str
dc.title.eng.fl_str_mv TRF1 as a major contributor for telomeres’ shortening in the context of obesity
title TRF1 as a major contributor for telomeres’ shortening in the context of obesity
spellingShingle TRF1 as a major contributor for telomeres’ shortening in the context of obesity
Aging
Obesity
Oxidative stress
Shelterin complex
Telomere length
TRF1
title_short TRF1 as a major contributor for telomeres’ shortening in the context of obesity
title_full TRF1 as a major contributor for telomeres’ shortening in the context of obesity
title_fullStr TRF1 as a major contributor for telomeres’ shortening in the context of obesity
title_full_unstemmed TRF1 as a major contributor for telomeres’ shortening in the context of obesity
title_sort TRF1 as a major contributor for telomeres’ shortening in the context of obesity
dc.creator.fl_str_mv Grun Kich, Lucas
Nevton Da Rosa, Teixeira
Mengden Von, Lúcia
De Bastiani, Marco Antônio
Parisi Migliorini, Mariana
Calixto Bortolin, Rafael
Lavandoski, Patrícia
Pierdona, Vinícius
Alves Biscaino, Letícia
Moreira Fonseca, José Cláudio
Mottin Corá, Cláudio
Jones, Marcus Herbert
Klamt, Fábio
Vontobel Padoin, Alexandre
Guma Costa R.Rodrigues, Fátima
Barbé Tuana, Florência María
dc.contributor.author.spa.fl_str_mv Grun Kich, Lucas
Nevton Da Rosa, Teixeira
Mengden Von, Lúcia
De Bastiani, Marco Antônio
Parisi Migliorini, Mariana
Calixto Bortolin, Rafael
Lavandoski, Patrícia
Pierdona, Vinícius
Alves Biscaino, Letícia
Moreira Fonseca, José Cláudio
Mottin Corá, Cláudio
Jones, Marcus Herbert
Klamt, Fábio
Vontobel Padoin, Alexandre
Guma Costa R.Rodrigues, Fátima
Barbé Tuana, Florência María
dc.subject.eng.fl_str_mv Aging
Obesity
Oxidative stress
Shelterin complex
Telomere length
TRF1
topic Aging
Obesity
Oxidative stress
Shelterin complex
Telomere length
TRF1
description Obesity is a prevalent multifactorial chronic disorder characterized by metabolic dysregulation. Sustained pro-oxidative mediators trigger harmful consequences that reflect at systemic level and contribute for the establishment of a premature senescent phenotype associated with macromolecular damage (DNA, protein, and lipids). Telomeres are structures that protect chromosome ends and are associated with a six-protein complex called the shelterin complex and subject to regulation. Under pro-oxidant conditions, telomere attrition and the altered expression of the shelterin proteins are central for the establishment of many pathophysiological conditions such as obesity. Thus, considering that individuals with obesity display a systemic oxidative stress profile that may compromise the telomeres length or its regulation, the aim of this study was to investigate telomere homeostasis in patients with obesity and explore broad/systemic associations with the expression of shelterin genes and the plasma redox state. We performed a cross-sectional study in 39 patients with obesity and 27 eutrophic subjects. Telomere length (T/S ratio) and gene expression of shelterin components were performed in peripheral blood mononuclear cells by qPCR.
publishDate 2018
dc.date.accessioned.none.fl_str_mv 2018-11-14T00:44:08Z
dc.date.available.none.fl_str_mv 2018-11-14T00:44:08Z
dc.date.issued.none.fl_str_mv 2018-09-24
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.content.spa.fl_str_mv Text
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
format http://purl.org/coar/resource_type/c_6501
status_str acceptedVersion
dc.identifier.issn.spa.fl_str_mv 08915849
dc.identifier.uri.spa.fl_str_mv http://hdl.handle.net/11323/932
dc.identifier.doi.spa.fl_str_mv DOI: 10.1016/j.freeradbiomed.2018.09.039
dc.identifier.instname.spa.fl_str_mv Corporación Universidad de la Costa
dc.identifier.reponame.spa.fl_str_mv REDICUC - Repositorio CUC
dc.identifier.repourl.spa.fl_str_mv https://repositorio.cuc.edu.co/
identifier_str_mv 08915849
DOI: 10.1016/j.freeradbiomed.2018.09.039
Corporación Universidad de la Costa
REDICUC - Repositorio CUC
url http://hdl.handle.net/11323/932
https://repositorio.cuc.edu.co/
dc.language.iso.none.fl_str_mv eng
language eng
dc.rights.spa.fl_str_mv Atribución – No comercial – Compartir igual
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución – No comercial – Compartir igual
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.publisher.spa.fl_str_mv Free Radical Biology And Medicine
institution Corporación Universidad de la Costa
bitstream.url.fl_str_mv https://repositorio.cuc.edu.co/bitstreams/6e288957-2eeb-4cbd-bcc9-4f5810ff5925/download
https://repositorio.cuc.edu.co/bitstreams/1f1b68bd-839b-41dc-b9c7-befa23637e00/download
https://repositorio.cuc.edu.co/bitstreams/ee6b62b5-ff36-494e-af80-0552c4b2d452/download
https://repositorio.cuc.edu.co/bitstreams/d9639d86-4816-4bfe-b92c-261f4a3b24f6/download
bitstream.checksum.fl_str_mv 3cc2e0af2fa7cb2b48dfa245b65ab651
8a4605be74aa9ea9d79846c1fba20a33
3f1b4ecd39664a84b4175aa8389a8c1e
61a86b31dcd9b5a68f3c68d6e25fc528
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio de la Universidad de la Costa CUC
repository.mail.fl_str_mv repdigital@cuc.edu.co
_version_ 1811760672730513408
spelling Grun Kich, LucasNevton Da Rosa, TeixeiraMengden Von, LúciaDe Bastiani, Marco AntônioParisi Migliorini, MarianaCalixto Bortolin, RafaelLavandoski, PatríciaPierdona, ViníciusAlves Biscaino, LetíciaMoreira Fonseca, José CláudioMottin Corá, CláudioJones, Marcus HerbertKlamt, FábioVontobel Padoin, AlexandreGuma Costa R.Rodrigues, FátimaBarbé Tuana, Florência María2018-11-14T00:44:08Z2018-11-14T00:44:08Z2018-09-2408915849http://hdl.handle.net/11323/932DOI: 10.1016/j.freeradbiomed.2018.09.039Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/Obesity is a prevalent multifactorial chronic disorder characterized by metabolic dysregulation. Sustained pro-oxidative mediators trigger harmful consequences that reflect at systemic level and contribute for the establishment of a premature senescent phenotype associated with macromolecular damage (DNA, protein, and lipids). Telomeres are structures that protect chromosome ends and are associated with a six-protein complex called the shelterin complex and subject to regulation. Under pro-oxidant conditions, telomere attrition and the altered expression of the shelterin proteins are central for the establishment of many pathophysiological conditions such as obesity. Thus, considering that individuals with obesity display a systemic oxidative stress profile that may compromise the telomeres length or its regulation, the aim of this study was to investigate telomere homeostasis in patients with obesity and explore broad/systemic associations with the expression of shelterin genes and the plasma redox state. We performed a cross-sectional study in 39 patients with obesity and 27 eutrophic subjects. Telomere length (T/S ratio) and gene expression of shelterin components were performed in peripheral blood mononuclear cells by qPCR.Grun Kich, Lucas-6a7dd59a-663d-4b52-9bda-b5c869fb7b64-0Nevton Da Rosa, Teixeira-fe0b8365-1afa-41a7-868d-368bb7a21b31-0Mengden Von, Lúcia-035ded7e-3335-4f7e-9201-93f305081c1a-0De Bastiani, Marco Antônio-06e45f6d-7cad-4b84-a5bb-4086c776b7f1-0Parisi Migliorini, Mariana-6bcd95c7-4ea9-40e1-bd65-71e6860b058a-0Calixto Bortolin, Rafael-0000-0003-4780-8499-600Lavandoski, Patrícia-d2d8b8e9-1ac2-43d7-a85c-7ef5e87c3941-0Pierdona, Vinícius-7d133ca0-a97f-4bf7-ae16-2ba18afb8c54-0Alves Biscaino, Letícia-0eb147fc-c9f8-4f3d-bd2c-d13e93db9f9c-0Moreira Fonseca, José Cláudio-4373df94-e345-42b4-861c-55953fd27b36-0Mottin Corá, Cláudio-4e7b7f71-dcf0-4b66-ba62-096aa76e8acb-0Jones, Marcus Herbert-2d3b247b-8515-4a74-97e6-33daa219d442-0Klamt, Fábio-6c9e5534-99d4-441c-a2c0-e4bbca7ce41e-0Vontobel Padoin, Alexandre-56927fc4-3d87-473a-988a-e19de80fd741-0Guma Costa R.Rodrigues, Fátima-8d422b94-54d7-4ffd-9986-eb749398d85c-0Barbé Tuana, Florência María-8bb037a3-e2e4-4e07-9137-654a385143af-0engFree Radical Biology And MedicineAtribución – No comercial – Compartir igualinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2AgingObesityOxidative stressShelterin complexTelomere lengthTRF1TRF1 as a major contributor for telomeres’ shortening in the context of obesityArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/acceptedVersionPublicationORIGINALTRF1 As A Major Contributor For Telomeres’.pdfTRF1 As A Major Contributor For Telomeres’.pdfapplication/pdf268620https://repositorio.cuc.edu.co/bitstreams/6e288957-2eeb-4cbd-bcc9-4f5810ff5925/download3cc2e0af2fa7cb2b48dfa245b65ab651MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.cuc.edu.co/bitstreams/1f1b68bd-839b-41dc-b9c7-befa23637e00/download8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILTRF1 As A Major Contributor For Telomeres’.pdf.jpgTRF1 As A Major Contributor For Telomeres’.pdf.jpgimage/jpeg61384https://repositorio.cuc.edu.co/bitstreams/ee6b62b5-ff36-494e-af80-0552c4b2d452/download3f1b4ecd39664a84b4175aa8389a8c1eMD54TEXTTRF1 As A Major Contributor For Telomeres’.pdf.txtTRF1 As A Major Contributor For Telomeres’.pdf.txttext/plain2821https://repositorio.cuc.edu.co/bitstreams/d9639d86-4816-4bfe-b92c-261f4a3b24f6/download61a86b31dcd9b5a68f3c68d6e25fc528MD5511323/932oai:repositorio.cuc.edu.co:11323/9322024-09-16 16:42:37.123open.accesshttps://repositorio.cuc.edu.coRepositorio de la Universidad de la Costa CUCrepdigital@cuc.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=