Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology

Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end,...

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Autores:
Wanda Rivero, Cintia
García, Natalia Soledad
Fernández-Lucas, Jesús
Betancor, Lorena
Romanelli, Gustavo
Trelles, Jorge Abel
Tipo de recurso:
Article of journal
Fecha de publicación:
2021
Institución:
Corporación Universidad de la Costa
Repositorio:
REDICUC - Repositorio CUC
Idioma:
eng
OAI Identifier:
oai:repositorio.cuc.edu.co:11323/8223
Acceso en línea:
https://hdl.handle.net/11323/8223
https://doi.org/10.3390/biom11050657
https://repositorio.cuc.edu.co/
Palabra clave:
Biomimetic silica
Enzyme immobilization
Glutaraldehyde
Entrapment
Calcium alginate
Antineoplastic drug
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openAccess
License
CC0 1.0 Universal
id RCUC2_9b8346610d37bfdc00102b4e75f84dee
oai_identifier_str oai:repositorio.cuc.edu.co:11323/8223
network_acronym_str RCUC2
network_name_str REDICUC - Repositorio CUC
repository_id_str
dc.title.spa.fl_str_mv Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
title Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
spellingShingle Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
Biomimetic silica
Enzyme immobilization
Glutaraldehyde
Entrapment
Calcium alginate
Antineoplastic drug
title_short Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
title_full Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
title_fullStr Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
title_full_unstemmed Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
title_sort Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology
dc.creator.fl_str_mv Wanda Rivero, Cintia
García, Natalia Soledad
Fernández-Lucas, Jesús
Betancor, Lorena
Romanelli, Gustavo
Trelles, Jorge Abel
dc.contributor.author.spa.fl_str_mv Wanda Rivero, Cintia
García, Natalia Soledad
Fernández-Lucas, Jesús
Betancor, Lorena
Romanelli, Gustavo
Trelles, Jorge Abel
dc.subject.spa.fl_str_mv Biomimetic silica
Enzyme immobilization
Glutaraldehyde
Entrapment
Calcium alginate
Antineoplastic drug
topic Biomimetic silica
Enzyme immobilization
Glutaraldehyde
Entrapment
Calcium alginate
Antineoplastic drug
description Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-05-04T23:32:56Z
dc.date.available.none.fl_str_mv 2021-05-04T23:32:56Z
dc.date.issued.none.fl_str_mv 2021-04-29
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.content.spa.fl_str_mv Text
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
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dc.identifier.issn.spa.fl_str_mv 2218-273X
dc.identifier.uri.spa.fl_str_mv https://hdl.handle.net/11323/8223
dc.identifier.doi.spa.fl_str_mv https://doi.org/10.3390/biom11050657
dc.identifier.instname.spa.fl_str_mv Corporación Universidad de la Costa
dc.identifier.reponame.spa.fl_str_mv REDICUC - Repositorio CUC
dc.identifier.repourl.spa.fl_str_mv https://repositorio.cuc.edu.co/
identifier_str_mv 2218-273X
Corporación Universidad de la Costa
REDICUC - Repositorio CUC
url https://hdl.handle.net/11323/8223
https://doi.org/10.3390/biom11050657
https://repositorio.cuc.edu.co/
dc.language.iso.none.fl_str_mv eng
language eng
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spelling Wanda Rivero, CintiaGarcía, Natalia SoledadFernández-Lucas, JesúsBetancor, LorenaRomanelli, GustavoTrelles, Jorge Abel2021-05-04T23:32:56Z2021-05-04T23:32:56Z2021-04-292218-273Xhttps://hdl.handle.net/11323/8223https://doi.org/10.3390/biom11050657Corporación Universidad de la CostaREDICUC - Repositorio CUChttps://repositorio.cuc.edu.co/Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.Wanda Rivero, CintiaGarcía, Natalia SoledadFernández-Lucas, Jesús-will be generated-orcid-0000-0001-7045-8306-600Betancor, LorenaRomanelli, Gustavo-will be generated-orcid-0000-0002-3529-4753-600Trelles, Jorge Abelapplication/pdfengCorporación Universidad de la CostaCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Biomoleculeshttps://www.mdpi.com/2218-273X/11/5/657Biomimetic silicaEnzyme immobilizationGlutaraldehydeEntrapmentCalcium alginateAntineoplastic drugGreen production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technologyArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/acceptedVersion1. 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[CrossRef]PublicationORIGINALGreen Production of Cladribine by Using Immobilized.pdfGreen Production of Cladribine by Using Immobilized.pdfapplication/pdf5148682https://repositorio.cuc.edu.co/bitstreams/67c475dd-de5e-4762-b0dc-cfd8a0f4e45a/download4066bc2acfdf1ee024b342f122ea23c0MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8701https://repositorio.cuc.edu.co/bitstreams/2b9de373-ff01-49d3-924f-01e9d1be4255/download42fd4ad1e89814f5e4a476b409eb708cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-83196https://repositorio.cuc.edu.co/bitstreams/cc9ff429-6dfb-42e7-975e-bffcce5f0fa6/downloade30e9215131d99561d40d6b0abbe9badMD53THUMBNAILGreen Production of Cladribine by Using Immobilized.pdf.jpgGreen Production of Cladribine by Using Immobilized.pdf.jpgimage/jpeg73662https://repositorio.cuc.edu.co/bitstreams/c51d94fa-ee2b-4c82-8cd7-cd69e83a4baf/downloadd14677645fd6f931c538e096d8b0f765MD54TEXTGreen Production of Cladribine by Using Immobilized.pdf.txtGreen 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