Green production of cladribine by using immobilized 2 0 -deoxyribosyltransferase from lactobacillus delbrueckii stabilized through a double covalent/entrapment technology

Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.

• Autores: Wanda Rivero, Cintia; García, Natalia Soledad; Fernández-Lucas, Jesús; Betancor, Lorena; Romanelli, Gustavo; Trelles, Jorge Abel
• Tipo de recurso: Article of journal
• Fecha de publicación: 2021
• Institución: Corporación Universidad de la Costa
• Repositorio: REDICUC - Repositorio CUC
• Idioma: eng
• OAI Identifier: oai:repositorio.cuc.edu.co:11323/8223
• Acceso en línea: https://hdl.handle.net/11323/8223; https://doi.org/10.3390/biom11050657; https://repositorio.cuc.edu.co/
• Palabra clave: Biomimetic silica; Enzyme immobilization; Glutaraldehyde; Entrapment; Calcium alginate; Antineoplastic drug
• Rights: openAccess
• License: CC0 1.0 Universal