This study evaluated lactic acid production through batch fermentation in a bioreactor with Thermoanaerobacter sp. strain USBA-018 and a chemically defined culture medium and with hydrolyzed pressed extract of Aloe vera peel (AHE). The strain USBA-018 fermented various sugars, but its primary end-pr...
- Autores:
-
Gómez-Gómez, Javier Antonio; Unidad de Saneamiento y Biotecnología Ambiental - Departamento de Biología – Facultad de Ciencias - Pontificia Universidad Javeriana, POB 56710, Bogotá D.C, Colombia
Giraldo-Estrada, Catalina; Departamento de Ingeniería de Procesos – Escuela de Ingeniería - Universidad EAFIT, Medellín, Colombia
Habeych, David; Verlengde Meeuwerderweg 97 9723ZM, Groningen, Netherlands
Baena, Sandra; Unidad de Saneamiento y Biotecnología Ambiental - Departamento de Biología – Facultad de Ciencias - Pontificia Universidad Javeriana, POB 56710, Bogotá D.C, Colombia
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2015
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31115
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15005
http://hdl.handle.net/10554/31115
- Palabra clave:
- Microbiology
Aloe vera, lactic acid, Thermoanaerobacter sp. USBA-018, thermophilic fermentation
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- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
Summary: | This study evaluated lactic acid production through batch fermentation in a bioreactor with Thermoanaerobacter sp. strain USBA-018 and a chemically defined culture medium and with hydrolyzed pressed extract of Aloe vera peel (AHE). The strain USBA-018 fermented various sugars, but its primary end-product was L-lactic acid. Factors which influenced L- lactic acid production were pH, addition of yeast extract (YE) and manganese chloride. Under the most favorable growing conditions for the production of lactic acid, yield (Yp/s) increased from 0.66 to 0.96 g/g with a productivity (Qp) of 0.62 g.l-1.h and a maximum lactic acid concentration of 178 mM at 26 hours of fermentation. When AHE was used, 93.3 mM, or 0.175 g.h/L, was obtained. These results show the potential for transformation of sugars that strain USBA-018 offers, but additional studies are needed to find out if different strategies using AHE as carbon source can produce large enough quantities of lactic acid to allow AHE to become a low-cost alternative substrate. |
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