Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome

Antiphospholipid syndrome (APS) affects coagulation and the brain by autoimmune mechanisms. The major antigen in APS is beta-2-glycoprotein I (?2-GPI) is known to complex with annexin A2 (ANXA2), and antibodies to ANXA2 have been described in APS. We measured these antibodies in mice with experiment...

Full description

Autores:
Tipo de recurso:
Fecha de publicación:
2017
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24279
Acceso en línea:
https://doi.org/10.1007/s12026-016-8840-8
https://repository.urosario.edu.co/handle/10336/24279
Palabra clave:
Beta2 glycoprotein 1
Immunoglobulin g
Lipocortin 2
Beta2 glycoprotein 1
Immunoglobulin g
Lipocortin 2
Phospholipid antibody
Animal experiment
Animal model
Animal tissue
Antiphospholipid syndrome
Article
Chemoluminescence
Controlled study
Cross reaction
Enzyme linked immunosorbent assay
Female
Immobilized metal affinity chromatography
Immune response
Immunization
Immunoblotting
Immunoprecipitation
Mouse
Nonhuman
Priority journal
Protein expression
Radioimmunoprecipitation
Animal
Antiphospholipid syndrome
Bagg albino mouse
Blood
Brain
Cross reaction
Disease model
Immunology
Animals
Annexin a2
Antiphospholipid syndrome
Beta 2-glycoprotein i
Brain
Cross reactions
Enzyme-linked immunosorbent assay
Female
Immunoglobulin g
Annexin
Anticardiolipin
Antiphospholipid syndrome
Autoantibodies
Autoimmune
Beta-2-glycoprotein
inbred balb c
animal
antiphospholipid
Antibodies
Disease models
Mice
Rights
License
Abierto (Texto Completo)
id EDOCUR2_fd391e2348045d0190921feab748cd20
oai_identifier_str oai:repository.urosario.edu.co:10336/24279
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling ed7d1209-7473-4837-a7b2-0324da8b775b-1151a1e7a-5cfc-4ff6-a835-9f6be68bdc7e-12195ba80-c74a-4944-a78a-1a96e7ebecb8-1232ce352-6420-4ab4-ac33-901ea6e15362-18e1b3c9b-cd9c-48e1-9332-38bae8acd747-18e6c26dd-7c93-449b-a6e6-fbae80578a2f-16b286b69-16c0-4eae-a12c-57f7a4b4c759-1e6b2d288-9ee7-4f09-b9ae-93d675eeb5af-12020-05-26T00:11:04Z2020-05-26T00:11:04Z2017Antiphospholipid syndrome (APS) affects coagulation and the brain by autoimmune mechanisms. The major antigen in APS is beta-2-glycoprotein I (?2-GPI) is known to complex with annexin A2 (ANXA2), and antibodies to ANXA2 have been described in APS. We measured these antibodies in mice with experimental APS (eAPS) induced by immunization with ?2-GPI. Sera of these mice reacted significantly with recombinant ANXA2 by enzyme-linked immunosorbent assay (ELISA) and the eAPS mice had significantly high levels of immunoglobulin G (IgG) in the brain by immunoblot assays compared to adjuvant immunized controls. Immunoprecipitation performed by mixing eAPS brain tissue with protein-G beads resulted in identification of two autoantigens unique to the eAPS group, one of which was ANXA2. In order to study more directly and methodically the specific role of anti-ANXA2 antibodies in APS, we immunized mice with ?2-GPI which contained no ANXA2 or with ANXA2 and measured antibodies to these proteins. Levels of antibodies to ANXA2 measured by ELISA were 0.72 ± 0.007 arbitrary units (a.u), 0.24 ± 0.03 and 0.02 ± 0.01 a.u for sera from ANXA2, ?2-GPI and control mice, respectively (p  less than  0.0001 and p = 0.037 for the comparison of the ANXA2 and ?2-GPI groups to the controls). Purified IgG from ?2-GPI sera did not show cross-binding with ANXA2. Antibodies to ?2-GPI and phospholipids were found in the ?2-GPI immunized group only. The present study suggests an immune response to the ?2-GPI–ANXA2 complex in eAPS and provides a novel ANXA2 immunization model which will serve to study the role of ANXA2 antibodies in of APS. © 2016, Springer Science+Business Media New York.application/pdfhttps://doi.org/10.1007/s12026-016-8840-80257277Xhttps://repository.urosario.edu.co/handle/10336/24279engHumana Press Inc.362No. 1355Immunologic ResearchVol. 65Immunologic Research, ISSN:0257277X, Vol.65, No.1 (2017); pp. 355-362https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979633320&doi=10.1007%2fs12026-016-8840-8&partnerID=40&md5=86b79ce9fd3350ff51b86519d6ba39d7Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURBeta2 glycoprotein 1Immunoglobulin gLipocortin 2Beta2 glycoprotein 1Immunoglobulin gLipocortin 2Phospholipid antibodyAnimal experimentAnimal modelAnimal tissueAntiphospholipid syndromeArticleChemoluminescenceControlled studyCross reactionEnzyme linked immunosorbent assayFemaleImmobilized metal affinity chromatographyImmune responseImmunizationImmunoblottingImmunoprecipitationMouseNonhumanPriority journalProtein expressionRadioimmunoprecipitationAnimalAntiphospholipid syndromeBagg albino mouseBloodBrainCross reactionDisease modelImmunologyAnimalsAnnexin a2Antiphospholipid syndromeBeta 2-glycoprotein iBrainCross reactionsEnzyme-linked immunosorbent assayFemaleImmunoglobulin gAnnexinAnticardiolipinAntiphospholipid syndromeAutoantibodiesAutoimmuneBeta-2-glycoproteininbred balb canimalantiphospholipidAntibodiesDisease modelsMiceCross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndromearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Weiss R.Bitton A.Nahary L.Arango M.T.Benhar I.Blank M.Shoenfeld Y.Chapman J.10336/24279oai:repository.urosario.edu.co:10336/242792022-05-02 07:37:21.664247https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome
title Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome
spellingShingle Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome
Beta2 glycoprotein 1
Immunoglobulin g
Lipocortin 2
Beta2 glycoprotein 1
Immunoglobulin g
Lipocortin 2
Phospholipid antibody
Animal experiment
Animal model
Animal tissue
Antiphospholipid syndrome
Article
Chemoluminescence
Controlled study
Cross reaction
Enzyme linked immunosorbent assay
Female
Immobilized metal affinity chromatography
Immune response
Immunization
Immunoblotting
Immunoprecipitation
Mouse
Nonhuman
Priority journal
Protein expression
Radioimmunoprecipitation
Animal
Antiphospholipid syndrome
Bagg albino mouse
Blood
Brain
Cross reaction
Disease model
Immunology
Animals
Annexin a2
Antiphospholipid syndrome
Beta 2-glycoprotein i
Brain
Cross reactions
Enzyme-linked immunosorbent assay
Female
Immunoglobulin g
Annexin
Anticardiolipin
Antiphospholipid syndrome
Autoantibodies
Autoimmune
Beta-2-glycoprotein
inbred balb c
animal
antiphospholipid
Antibodies
Disease models
Mice
title_short Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome
title_full Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome
title_fullStr Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome
title_full_unstemmed Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome
title_sort Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome
dc.subject.keyword.spa.fl_str_mv Beta2 glycoprotein 1
Immunoglobulin g
Lipocortin 2
Beta2 glycoprotein 1
Immunoglobulin g
Lipocortin 2
Phospholipid antibody
Animal experiment
Animal model
Animal tissue
Antiphospholipid syndrome
Article
Chemoluminescence
Controlled study
Cross reaction
Enzyme linked immunosorbent assay
Female
Immobilized metal affinity chromatography
Immune response
Immunization
Immunoblotting
Immunoprecipitation
Mouse
Nonhuman
Priority journal
Protein expression
Radioimmunoprecipitation
Animal
Antiphospholipid syndrome
Bagg albino mouse
Blood
Brain
Cross reaction
Disease model
Immunology
Animals
Annexin a2
Antiphospholipid syndrome
Beta 2-glycoprotein i
Brain
Cross reactions
Enzyme-linked immunosorbent assay
Female
Immunoglobulin g
Annexin
Anticardiolipin
Antiphospholipid syndrome
Autoantibodies
Autoimmune
Beta-2-glycoprotein
topic Beta2 glycoprotein 1
Immunoglobulin g
Lipocortin 2
Beta2 glycoprotein 1
Immunoglobulin g
Lipocortin 2
Phospholipid antibody
Animal experiment
Animal model
Animal tissue
Antiphospholipid syndrome
Article
Chemoluminescence
Controlled study
Cross reaction
Enzyme linked immunosorbent assay
Female
Immobilized metal affinity chromatography
Immune response
Immunization
Immunoblotting
Immunoprecipitation
Mouse
Nonhuman
Priority journal
Protein expression
Radioimmunoprecipitation
Animal
Antiphospholipid syndrome
Bagg albino mouse
Blood
Brain
Cross reaction
Disease model
Immunology
Animals
Annexin a2
Antiphospholipid syndrome
Beta 2-glycoprotein i
Brain
Cross reactions
Enzyme-linked immunosorbent assay
Female
Immunoglobulin g
Annexin
Anticardiolipin
Antiphospholipid syndrome
Autoantibodies
Autoimmune
Beta-2-glycoprotein
inbred balb c
animal
antiphospholipid
Antibodies
Disease models
Mice
dc.subject.keyword.eng.fl_str_mv inbred balb c
animal
antiphospholipid
Antibodies
Disease models
Mice
description Antiphospholipid syndrome (APS) affects coagulation and the brain by autoimmune mechanisms. The major antigen in APS is beta-2-glycoprotein I (?2-GPI) is known to complex with annexin A2 (ANXA2), and antibodies to ANXA2 have been described in APS. We measured these antibodies in mice with experimental APS (eAPS) induced by immunization with ?2-GPI. Sera of these mice reacted significantly with recombinant ANXA2 by enzyme-linked immunosorbent assay (ELISA) and the eAPS mice had significantly high levels of immunoglobulin G (IgG) in the brain by immunoblot assays compared to adjuvant immunized controls. Immunoprecipitation performed by mixing eAPS brain tissue with protein-G beads resulted in identification of two autoantigens unique to the eAPS group, one of which was ANXA2. In order to study more directly and methodically the specific role of anti-ANXA2 antibodies in APS, we immunized mice with ?2-GPI which contained no ANXA2 or with ANXA2 and measured antibodies to these proteins. Levels of antibodies to ANXA2 measured by ELISA were 0.72 ± 0.007 arbitrary units (a.u), 0.24 ± 0.03 and 0.02 ± 0.01 a.u for sera from ANXA2, ?2-GPI and control mice, respectively (p  less than  0.0001 and p = 0.037 for the comparison of the ANXA2 and ?2-GPI groups to the controls). Purified IgG from ?2-GPI sera did not show cross-binding with ANXA2. Antibodies to ?2-GPI and phospholipids were found in the ?2-GPI immunized group only. The present study suggests an immune response to the ?2-GPI–ANXA2 complex in eAPS and provides a novel ANXA2 immunization model which will serve to study the role of ANXA2 antibodies in of APS. © 2016, Springer Science+Business Media New York.
publishDate 2017
dc.date.created.spa.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:11:04Z
dc.date.available.none.fl_str_mv 2020-05-26T00:11:04Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1007/s12026-016-8840-8
dc.identifier.issn.none.fl_str_mv 0257277X
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/24279
url https://doi.org/10.1007/s12026-016-8840-8
https://repository.urosario.edu.co/handle/10336/24279
identifier_str_mv 0257277X
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 362
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationStartPage.none.fl_str_mv 355
dc.relation.citationTitle.none.fl_str_mv Immunologic Research
dc.relation.citationVolume.none.fl_str_mv Vol. 65
dc.relation.ispartof.spa.fl_str_mv Immunologic Research, ISSN:0257277X, Vol.65, No.1 (2017); pp. 355-362
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979633320&doi=10.1007%2fs12026-016-8840-8&partnerID=40&md5=86b79ce9fd3350ff51b86519d6ba39d7
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Humana Press Inc.
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
_version_ 1814167591714816000