The H3K9 methylation writer SETDB1 and its reader MPP8 cooperate to silence satellite DNA repeats in mouse embryonic stem cells
SETDB1 (SET Domain Bifurcated histone lysine methyltransferase 1) is a key lysine methyltransferase (KMT) required in embryonic stem cells (ESCs), where it silences transposable elements and DNA repeats via histone H3 lysine 9 tri-methylation (H3K9me3), independently of DNA methylation. The H3K9 met...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/24143
- Acceso en línea:
- https://doi.org/10.3390/genes10100750
https://repository.urosario.edu.co/handle/10336/24143
- Palabra clave:
- Histone H3
Histone lysine methyltransferase
M phase phosphoprotein 8
Phosphoprotein
Repetitive DNA
Satellite DNA
SET domain bifurcated histone lysine methyltransferase 1
Small interfering RNA
Unclassified drug
Histone
Histone lysine methyltransferase
Phosphoprotein
Protein binding
Satellite DNA
Animal cell
Article
Binding site
Biochemical analysis
Cell cycle M phase
Chromatin immunoprecipitation
Comparative study
Complex formation
Controlled study
Down regulation
Embryo
Gene
Gene control
Gene expression
Gene expression regulation
Gene function
Gene number
Gene silencing
Gene targeting
Genomics
Hela S3 cell line
Histone methylation
Immunoprecipitation
Long interspersed nuclear element
Loss of function mutation
Molecular genetics
Mouse
Mouse embryonic stem cell
MPP8 gene
Nonhuman
Protein processing
Protein protein interaction
RNA sequence
SETDB1 gene
Short interspersed nuclear element
Transcriptomics
Upregulation
Animal
Cell culture
Genetics
Hela cell line
Human
Metabolism
Mouse embryonic stem cell
Animals
Hela Cells
Histone-Lysine N-Methyltransferase
Histones
Humans
Mice
Mouse Embryonic Stem Cells
Phosphoproteins
Protein Binding
Histone lysine methylation
M-phase phosphoprotein 8
Satellite DNA repeats
SETDB1/KMT1E
Transcription
Cultured
mouse
mouse
Satellite
Mphosph8 protein
SETDB1 protein
Cells
DNA
- Rights
- License
- Abierto (Texto Completo)
Summary: | SETDB1 (SET Domain Bifurcated histone lysine methyltransferase 1) is a key lysine methyltransferase (KMT) required in embryonic stem cells (ESCs), where it silences transposable elements and DNA repeats via histone H3 lysine 9 tri-methylation (H3K9me3), independently of DNA methylation. The H3K9 methylation reader M-Phase Phosphoprotein 8 (MPP8) is highly expressed in ESCs and germline cells. Although evidence of a cooperation between H3K9 KMTs and MPP8 in committed cells has emerged, the interplay between H3K9 methylation writers and MPP8 in ESCs remains elusive. Here, we show that MPP8 interacts physically and functionally with SETDB1 in ESCs. Indeed, combining biochemical, transcriptomic and genomic analyses, we found that MPP8 and SETDB1 co-regulate a significant number of common genomic targets, especially the DNA satellite repeats. Together, our data point to a model in which the silencing of a class of repeated sequences in ESCs involves the cooperation between the H3K9 methylation writer SETDB1 and its reader MPP8. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. |
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