Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model

We have covalently conjugated an N-terminus Plasmodium vivax apical membrane antigen-1 (AMA-1) peptide to functionalized carbon nanotubes (f-CNT). Immunological characterization of this molecular conjugate revealed that the immunogen-AMA-1 peptide was appropriately presented after being conjugated to CNTs as well as being recognized by BALB/c polyclonal antibodies. Subsequent experiments lead us to assess the AMA-1 peptide alone, as well as the CNT-peptide conjugate regarding rodent malarial infection. Remarkably, the peptide effectively controlled and delayed Plasmodium berghei-challenged animals' parasitaemia. The peptide-CNT conjugate displayed similar immunological properties to the peptide alone by protecting or delaying malarial infection. The peptide presentation by f-CNT to the immune system thus constitutes a promising approach for synthetic malarial vaccine formulation since the immunogen peptide conformation is well preserved. © 2008 Elsevier Ltd. All rights reserved.

• Fecha de publicación: 2008
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23447
• Acceso en línea: https://doi.org/10.1016/j.vaccine.2008.08.014; https://repository.urosario.edu.co/handle/10336/23447
• Palabra clave: Apical membrane antigen 1; Carbon nanotube; Drug carrier; Polyclonal antibody; Amino terminal sequence; Animal experiment; Animal model; Article; Bagg albino mouse; Conjugation; Controlled study; Drug delivery system; Drug structure; Female; Immune system; Immunogenicity; Malaria; Mouse; Nonhuman; Parasitemia; Plasmodium berghei; Plasmodium vivax; Priority journal; Alleles; Amino acid sequence; Animals; Cytokines; Drug delivery systems; Enzyme-linked immunosorbent assay; Female; Fluorescent antibody technique; Hla-dr antigens; Malaria; Malaria vaccines; Membrane proteins; Mice; Molecular sequence data; Nanotubes; Plasmodium berghei; Plasmodium vivax; Protozoan proteins; Apical membrane antigen 1 (ama-1); Functionalized carbon nanotubes (f-cnt); Synthetic vaccine delivery system; protozoan; mhc class ii; subunit; western; inbred balb c; electron; Antigens; Blotting; Genes; Mice; Microscopy; Vaccines
• License: Abierto (Texto Completo)