Analytical performance of Four Polymerase Chain Reaction (PCR) and real time PCR (qPCR) assays for the detection of six Leishmania species DNA in Colombia

Leishmaniasis comprises a spectrum of parasitic diseases caused by protozoans of the genus Leishmania. Molecular tools have been widely employed for the detection of Leishmania due to its high sensitivity and specificity. However, the analytical performance of molecular platforms as PCR and real tim...

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Autores:

Tipo de recurso:

Fecha de publicación:

2017

Institución:

Universidad del Rosario

Repositorio:

Repositorio EdocUR - U. Rosario

Idioma:

eng

OAI Identifier:

oai:repository.urosario.edu.co:10336/24094

Acceso en línea:

https://doi.org/10.3389/fmicb.2017.01907
https://repository.urosario.edu.co/handle/10336/24094

Palabra clave:

Heat shock protein 70
Kinetoplast dna
Molecular marker
Rna 18s
Analytical parameters
Anticipated reportable range
Article
Colombia
Controlled study
Dna extraction
Gene amplification
Leishmania
Limit of detection
Measurement accuracy
Microorganism detection
Nonhuman
Polymerase chain reaction
Post hoc analysis
Quantitative analysis
Real time polymerase chain reaction
Reproducibility
Sensitivity and specificity
Analytical performance
Leishmania
Molecular diagnosis
Pcr
Qpcr

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