A combined linkage, microarray and exome analysis suggests MAP3K11 as a candidate gene for left ventricular hypertrophy

Background: Electrocardiographic measures of left ventricular hypertrophy (LVH) are used as predictors of cardiovascular risk. We combined linkage and association analyses to discover novel rare genetic variants involved in three such measures and two principal components derived from them. Methods: The study was conducted among participants from the Erasmus Rucphen Family Study (ERF), a Dutch family-based sample from the southwestern Netherlands. Variance components linkage analyses were performed using Merlin. Regions of interest (LOD > 1.9) were fine-mapped using microarray and exome sequence data. Results: We observed one significant LOD score for the second principal component on chromosome 15 (LOD score = 3.01) and 12 suggestive LOD scores. Several loci contained variants identified in GWAS for these traits; however, these did not explain the linkage peaks, nor did other common variants. Exome sequence data identified two associated variants after multiple testing corrections were applied. Conclusions: We did not find common SNPs explaining these linkage signals. Exome sequencing uncovered a relatively rare variant in MAPK3K11 on chromosome 11 (MAF = 0.01) that helped account for the suggestive linkage peak observed for the first principal component. Conditional analysis revealed a drop in LOD from 2.01 to 0.88 for MAP3K11, suggesting that this variant may partially explain the linkage signal at this chromosomal location. MAP3K11 is related to the JNK pathway and is a pro-apoptotic kinase that plays an important role in the induction of cardiomyocyte apoptosis in various pathologies, including LVH. © 2018 The Author(s).

• Fecha de publicación: 2018
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/20386
• Acceso en línea: https://doi.org/10.1186/s12920-018-0339-9; https://repository.urosario.edu.co/handle/10336/20386
• Palabra clave: Chromosome 11; Chromosome 15; Dutchman; Exome Analysis; Gene; Gene Locus; Genetic Analysis; Genetic Association; Genetic Variability; Heart Left Ventricle Hypertrophy; Human; Limit Of Detection; Linkage Analysis; Major Clinical Study; Male; Map3K11 Gene; Microarray Analysis; Netherlands; Priority Journal; Whole Exome Sequencing; Cromosoma 11; Cromosoma 15; Enfermedades; Adult; Article; Female; Middle Aged; Cardiomegalia; Enfermedades cardiovasculares
• License: Abierto (Texto Completo)