Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells
Background: In diagnostic pathology, HER2 status is determined in interphase nuclei by fluorescence in situ hybridization (FISH) with probes for the HER2 gene and for the chromosome 17 centromere (CEP17). The latter probe is used as a surrogate for chromosome 17 copies, however chromosome 17 (Chr17)...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2014
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/23559
- Acceso en línea:
- https://doi.org/10.1186/1471-2407-14-922
https://repository.urosario.edu.co/handle/10336/23559
- Palabra clave:
- Epidermal growth factor receptor 2
Carrier protein
Dna binding protein
Dna topoisomerase (atp hydrolysing)
Dna topoisomerase ii alpha
Membrane protein
Tumor antigen
Amplicon
Article
Breast cancer cell line
Bt474 cell line
Centromere
Chromosome 11
Chromosome 17
Chromosome 8
Chromosome g band
Chromosome rearrangement
Chromosome translocation
Fluorescence in situ hybridization
Gene location
Gene mapping
Human
Human tissue
Interphase
Investigative procedures
Jimt 1 cell line
Karyotyping
Kpl4 cell line
Mcf 7 cell line
Mda mb231 cell line
Mda mb361 cell line
Multicolor fluorescence in situ hybridization
Oncogene
Oncogene neu
Polysome
Signal transduction
Skbr3 cell line
Stard3 gene
T47d cell line
Tnbc case cell line
Top2a gene
Triple negative breast cancer
Zr 75 1 cell line
Carcinoma
Cell nucleus
Centromere
Cytology
Fluorescence in situ hybridization
Gene translocation
Genetics
Interphase
Karyotyping
Metaphase
Procedures
Proto oncogene
Tumor cell line
Carcinoma
Carrier proteins
Cell nucleus
Centromere
Dna-binding proteins
Humans
Interphase
Karyotyping
Membrane proteins
Metaphase
Triple negative breast neoplasms
Breast cancer
Cep17
Chromosomal rearrangements
Chromosome 17
Her2
M-fish
Polysomy
Stard3
Top2a
human
type ii
tumor
neoplasm
genetic
fluorescence
erbb-2
human
pair 17
Stard3 protein
Antigens
Cell line
Chromosomes
Dna topoisomerases
Genes
In situ hybridization
Translocation
- Rights
- License
- Abierto (Texto Completo)
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oai:repository.urosario.edu.co:10336/23559 |
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Repositorio EdocUR - U. Rosario |
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|
dc.title.spa.fl_str_mv |
Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells |
title |
Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells |
spellingShingle |
Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells Epidermal growth factor receptor 2 Carrier protein Dna binding protein Dna topoisomerase (atp hydrolysing) Dna topoisomerase ii alpha Membrane protein Tumor antigen Amplicon Article Breast cancer cell line Bt474 cell line Centromere Chromosome 11 Chromosome 17 Chromosome 8 Chromosome g band Chromosome rearrangement Chromosome translocation Fluorescence in situ hybridization Gene location Gene mapping Human Human tissue Interphase Investigative procedures Jimt 1 cell line Karyotyping Kpl4 cell line Mcf 7 cell line Mda mb231 cell line Mda mb361 cell line Multicolor fluorescence in situ hybridization Oncogene Oncogene neu Polysome Signal transduction Skbr3 cell line Stard3 gene T47d cell line Tnbc case cell line Top2a gene Triple negative breast cancer Zr 75 1 cell line Carcinoma Cell nucleus Centromere Cytology Fluorescence in situ hybridization Gene translocation Genetics Interphase Karyotyping Metaphase Procedures Proto oncogene Tumor cell line Carcinoma Carrier proteins Cell nucleus Centromere Dna-binding proteins Humans Interphase Karyotyping Membrane proteins Metaphase Triple negative breast neoplasms Breast cancer Cep17 Chromosomal rearrangements Chromosome 17 Her2 M-fish Polysomy Stard3 Top2a human type ii tumor neoplasm genetic fluorescence erbb-2 human pair 17 Stard3 protein Antigens Cell line Chromosomes Dna topoisomerases Genes In situ hybridization Translocation |
title_short |
Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells |
title_full |
Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells |
title_fullStr |
Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells |
title_full_unstemmed |
Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells |
title_sort |
Unraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cells |
dc.subject.keyword.spa.fl_str_mv |
Epidermal growth factor receptor 2 Carrier protein Dna binding protein Dna topoisomerase (atp hydrolysing) Dna topoisomerase ii alpha Membrane protein Tumor antigen Amplicon Article Breast cancer cell line Bt474 cell line Centromere Chromosome 11 Chromosome 17 Chromosome 8 Chromosome g band Chromosome rearrangement Chromosome translocation Fluorescence in situ hybridization Gene location Gene mapping Human Human tissue Interphase Investigative procedures Jimt 1 cell line Karyotyping Kpl4 cell line Mcf 7 cell line Mda mb231 cell line Mda mb361 cell line Multicolor fluorescence in situ hybridization Oncogene Oncogene neu Polysome Signal transduction Skbr3 cell line Stard3 gene T47d cell line Tnbc case cell line Top2a gene Triple negative breast cancer Zr 75 1 cell line Carcinoma Cell nucleus Centromere Cytology Fluorescence in situ hybridization Gene translocation Genetics Interphase Karyotyping Metaphase Procedures Proto oncogene Tumor cell line Carcinoma Carrier proteins Cell nucleus Centromere Dna-binding proteins Humans Interphase Karyotyping Membrane proteins Metaphase Triple negative breast neoplasms Breast cancer Cep17 Chromosomal rearrangements Chromosome 17 Her2 M-fish Polysomy Stard3 Top2a |
topic |
Epidermal growth factor receptor 2 Carrier protein Dna binding protein Dna topoisomerase (atp hydrolysing) Dna topoisomerase ii alpha Membrane protein Tumor antigen Amplicon Article Breast cancer cell line Bt474 cell line Centromere Chromosome 11 Chromosome 17 Chromosome 8 Chromosome g band Chromosome rearrangement Chromosome translocation Fluorescence in situ hybridization Gene location Gene mapping Human Human tissue Interphase Investigative procedures Jimt 1 cell line Karyotyping Kpl4 cell line Mcf 7 cell line Mda mb231 cell line Mda mb361 cell line Multicolor fluorescence in situ hybridization Oncogene Oncogene neu Polysome Signal transduction Skbr3 cell line Stard3 gene T47d cell line Tnbc case cell line Top2a gene Triple negative breast cancer Zr 75 1 cell line Carcinoma Cell nucleus Centromere Cytology Fluorescence in situ hybridization Gene translocation Genetics Interphase Karyotyping Metaphase Procedures Proto oncogene Tumor cell line Carcinoma Carrier proteins Cell nucleus Centromere Dna-binding proteins Humans Interphase Karyotyping Membrane proteins Metaphase Triple negative breast neoplasms Breast cancer Cep17 Chromosomal rearrangements Chromosome 17 Her2 M-fish Polysomy Stard3 Top2a human type ii tumor neoplasm genetic fluorescence erbb-2 human pair 17 Stard3 protein Antigens Cell line Chromosomes Dna topoisomerases Genes In situ hybridization Translocation |
dc.subject.keyword.eng.fl_str_mv |
human type ii tumor neoplasm genetic fluorescence erbb-2 human pair 17 Stard3 protein Antigens Cell line Chromosomes Dna topoisomerases Genes In situ hybridization Translocation |
description |
Background: In diagnostic pathology, HER2 status is determined in interphase nuclei by fluorescence in situ hybridization (FISH) with probes for the HER2 gene and for the chromosome 17 centromere (CEP17). The latter probe is used as a surrogate for chromosome 17 copies, however chromosome 17 (Chr17) is frequently rearranged. The frequency and type of specific structural Chr17 alterations in breast cancer have been studied by using comparative genomic hybridization and spectral karyotyping, but not fully detailed. Actually, balanced chromosome rearrangements (e.g. translocations or inversions) and low frequency mosaicisms are assessable on metaphases using G-banding karyotype and multicolor FISH (M-FISH) only. Methods: We sought to elucidate the CEP17 and HER2 FISH patterns of interphase nuclei by evaluating Chr17 rearrangements in metaphases of 9 breast cancer cell lines and a primary culture from a triple negative breast carcinoma by using G-banding, FISH and M-FISH. Results: Thirty-nine rearranged chromosomes containing a portion of Chr17 were observed. Chromosomes 8 and 11 were the most frequent partners of Chr17 translocations. The lowest frequency of Chr17 abnormalities was observed in the HER2-negative cell lines, while the highest was observed in the HER2-positive SKBR3 cells. The MDA-MB231 triple negative cell line was the sole to show only non-altered copies of Chr17, while the SKBR3, MDA-MB361 and JIMT-1 HER2-positive cells carried no normal Chr17 copies. True polysomy was observed in MDA-MB231 as the only Chr17 alteration. In BT474 cells polysomy was associated to Chr17 structural alterations. By comparing M-FISH and FISH data, in 8 out of 39 rearranged chromosomes only CEP17 signals were detectable, whereas in 14 rearranged chromosomes HER2 and STARD3 genes were present without CEP17 signals. HER2 and STARD3 always co-localized on the same chromosomes and were always co-amplified, whereas TOP2A also mapped to different derivatives and was co-amplified with HER2 and STARD3 on SKBR3 cells only. Conclusion: The high frequency of complex Chr17 abnormalities suggests that the interpretation of FISH results on interphase nuclei using a dual probe assay to assess gene amplification should be performed 'with caution', given that CEP17 signals are not always indicative of normal unaltered or rearranged copies of Chr17. © 2014 Rondón-Lagos et al. |
publishDate |
2014 |
dc.date.created.spa.fl_str_mv |
2014 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:03:05Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:03:05Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1186/1471-2407-14-922 |
dc.identifier.issn.none.fl_str_mv |
14712407 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/23559 |
url |
https://doi.org/10.1186/1471-2407-14-922 https://repository.urosario.edu.co/handle/10336/23559 |
identifier_str_mv |
14712407 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationTitle.none.fl_str_mv |
BMC Cancer |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 14 |
dc.relation.ispartof.spa.fl_str_mv |
BMC Cancer, ISSN:14712407, Vol.14, No.1 (2014) |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924406034&doi=10.1186%2f1471-2407-14-922&partnerID=40&md5=15a4b472ef70231b4612d202839a542e |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
BioMed Central Ltd. |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
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9fca649b-66f9-4d26-9d5f-6221a6d4aef99912a804-e6e8-4aef-8452-e716edae78e4e14757fa-5d19-4f7b-bb3d-f71a50e6feca51aa8cf3-5ea0-4440-ab68-dabc923f57e25186625160003b55366-250f-42c2-8c6c-a48c225c461781a757f0-50ef-49c7-97f3-ac1c53427dce2d7e8bea-4138-447d-b84c-0182522e38612020-05-26T00:03:05Z2020-05-26T00:03:05Z2014Background: In diagnostic pathology, HER2 status is determined in interphase nuclei by fluorescence in situ hybridization (FISH) with probes for the HER2 gene and for the chromosome 17 centromere (CEP17). The latter probe is used as a surrogate for chromosome 17 copies, however chromosome 17 (Chr17) is frequently rearranged. The frequency and type of specific structural Chr17 alterations in breast cancer have been studied by using comparative genomic hybridization and spectral karyotyping, but not fully detailed. Actually, balanced chromosome rearrangements (e.g. translocations or inversions) and low frequency mosaicisms are assessable on metaphases using G-banding karyotype and multicolor FISH (M-FISH) only. Methods: We sought to elucidate the CEP17 and HER2 FISH patterns of interphase nuclei by evaluating Chr17 rearrangements in metaphases of 9 breast cancer cell lines and a primary culture from a triple negative breast carcinoma by using G-banding, FISH and M-FISH. Results: Thirty-nine rearranged chromosomes containing a portion of Chr17 were observed. Chromosomes 8 and 11 were the most frequent partners of Chr17 translocations. The lowest frequency of Chr17 abnormalities was observed in the HER2-negative cell lines, while the highest was observed in the HER2-positive SKBR3 cells. The MDA-MB231 triple negative cell line was the sole to show only non-altered copies of Chr17, while the SKBR3, MDA-MB361 and JIMT-1 HER2-positive cells carried no normal Chr17 copies. True polysomy was observed in MDA-MB231 as the only Chr17 alteration. In BT474 cells polysomy was associated to Chr17 structural alterations. By comparing M-FISH and FISH data, in 8 out of 39 rearranged chromosomes only CEP17 signals were detectable, whereas in 14 rearranged chromosomes HER2 and STARD3 genes were present without CEP17 signals. HER2 and STARD3 always co-localized on the same chromosomes and were always co-amplified, whereas TOP2A also mapped to different derivatives and was co-amplified with HER2 and STARD3 on SKBR3 cells only. Conclusion: The high frequency of complex Chr17 abnormalities suggests that the interpretation of FISH results on interphase nuclei using a dual probe assay to assess gene amplification should be performed 'with caution', given that CEP17 signals are not always indicative of normal unaltered or rearranged copies of Chr17. © 2014 Rondón-Lagos et al.application/pdfhttps://doi.org/10.1186/1471-2407-14-92214712407https://repository.urosario.edu.co/handle/10336/23559engBioMed Central Ltd.No. 1BMC CancerVol. 14BMC Cancer, ISSN:14712407, Vol.14, No.1 (2014)https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924406034&doi=10.1186%2f1471-2407-14-922&partnerID=40&md5=15a4b472ef70231b4612d202839a542eAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocUREpidermal growth factor receptor 2Carrier proteinDna binding proteinDna topoisomerase (atp hydrolysing)Dna topoisomerase ii alphaMembrane proteinTumor antigenAmpliconArticleBreast cancer cell lineBt474 cell lineCentromereChromosome 11Chromosome 17Chromosome 8Chromosome g bandChromosome rearrangementChromosome translocationFluorescence in situ hybridizationGene locationGene mappingHumanHuman tissueInterphaseInvestigative proceduresJimt 1 cell lineKaryotypingKpl4 cell lineMcf 7 cell lineMda mb231 cell lineMda mb361 cell lineMulticolor fluorescence in situ hybridizationOncogeneOncogene neuPolysomeSignal transductionSkbr3 cell lineStard3 geneT47d cell lineTnbc case cell lineTop2a geneTriple negative breast cancerZr 75 1 cell lineCarcinomaCell nucleusCentromereCytologyFluorescence in situ hybridizationGene translocationGeneticsInterphaseKaryotypingMetaphaseProceduresProto oncogeneTumor cell lineCarcinomaCarrier proteinsCell nucleusCentromereDna-binding proteinsHumansInterphaseKaryotypingMembrane proteinsMetaphaseTriple negative breast neoplasmsBreast cancerCep17Chromosomal rearrangementsChromosome 17Her2M-fishPolysomyStard3Top2ahumantype iitumorneoplasmgeneticfluorescenceerbb-2humanpair 17Stard3 proteinAntigensCell lineChromosomesDna topoisomerasesGenesIn situ hybridizationTranslocationUnraveling the chromosome 17 patterns of FISH in interphase nuclei: An in-depth analysis of the HER2 amplicon and chromosome 17 centromere by karyotyping, FISH and M-FISH in breast cancer cellsarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Rondón-Lagos, MilenaVerdun Di Cantogno, LudovicaRangel, NelsonMele, TeresaRamírez Clavijo, Sandra RocíoScagliotti, GiorgioMarchiò, CaterinaSapino, AnnaORIGINAL1471-2407-14-922.pdfapplication/pdf1980306https://repository.urosario.edu.co/bitstreams/da1f079a-79de-41ac-9d10-b7b4c6757685/download1e18b7ca905d558b5470d0a16ed0bc9aMD51TEXT1471-2407-14-922.pdf.txt1471-2407-14-922.pdf.txtExtracted texttext/plain51448https://repository.urosario.edu.co/bitstreams/8557ab27-db02-4777-ac20-5b47fb0346c9/download982dfea6c1de9f14d32d6a01f7ac0173MD52THUMBNAIL1471-2407-14-922.pdf.jpg1471-2407-14-922.pdf.jpgGenerated Thumbnailimage/jpeg4398https://repository.urosario.edu.co/bitstreams/909c4ae2-9064-4c2f-aefb-38b362c6b150/download6fc3fc508343c8e61936d26991e5de18MD5310336/23559oai:repository.urosario.edu.co:10336/235592022-05-02 07:37:16.618563https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |