Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis
Antigen structure modulation represents an approach towards designing subunit malaria vaccines. A specific epitope's ? carbon stereochemistry, as well as its backbone topochemistry, was assessed for obtaining novel malarial immunogens. A variety of MSP-138–61 Plasmodium falciparum epitope pseud...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2004
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/28112
- Acceso en línea:
- https://doi.org/10.1016/j.jsb.2004.04.010
https://repository.urosario.edu.co/handle/10336/28112
- Palabra clave:
- Molecular mimicry
Merozoite surface protein-1 (MSP-1)
Pseudopeptide
Topology
Stereochemistry
- Rights
- License
- Restringido (Acceso a grupos específicos)
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Repositorio EdocUR - U. Rosario |
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ed02648e-d655-4bbd-8d6e-90ad3efa4387-1b5fe42f7-3f26-4cca-b3fa-1d3fad317bfb-151848826-1dbe17748-4c0d-479b-ac09-27bf4a63b6e5-1fa33b517-50cf-4bb5-a980-3773caf84cd8-13426bc6d-5d8d-4b50-aa8f-ff9d9929eac2-11450e86b-c3ae-413a-bffa-4caa6fae204a-19e3ba9df-fe89-48fe-9521-cc8f452d56f5-12020-08-19T14:45:51Z2020-08-19T14:45:51Z2004-10-01Antigen structure modulation represents an approach towards designing subunit malaria vaccines. A specific epitope's ? carbon stereochemistry, as well as its backbone topochemistry, was assessed for obtaining novel malarial immunogens. A variety of MSP-138–61 Plasmodium falciparum epitope pseudopeptides derived were synthesised, based on solid-phase pseudopeptide chemistry strategies; these included all-l, all-d, partially-d substituted, all-?-[NH-CO]-Retro, all-?-[NH-CO]-Retro-inverso, and ?-[CH2NH] reduced amide surrogates. We demonstrate that specific recombinant MSP-134–469 fragment binding to red blood cells (RBCs) is specifically inhibited by non-modified MSP-142–61, as well as by its V52-L53, M51-V52 reduced amide surrogates and partial-d substitutions in K48 and E49. In vivo tests revealed that reduced amide pseudopeptide-immunised Aotus monkeys induced neutralising antibodies specifically recognising the MSP-1 N-terminus region. These findings support the role of molecular conformation in malaria vaccine development.application/pdfhttps://doi.org/10.1016/j.jsb.2004.04.010ISSN: 1047-8477EISSN: 1095-8657https://repository.urosario.edu.co/handle/10336/28112engElsevier122No. 1110Journal of Structural BiologyVol. 148Journal of Structural Biology, ISSN: 1047-8477;EISSN: 1095-8657, Vol.148, No.1 (2004); pp. 110-122https://www.sciencedirect.com/science/article/pii/S1047847704001030Restringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecJournal of Structural Biologyinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMolecular mimicryMerozoite surface protein-1 (MSP-1)PseudopeptideTopologyStereochemistryMapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysisMapeo de la anatomía de un epítopo de Plasmodium falciparum MSP-1 usando anticuerpos mono y policlonales inducidos por pseudopéptidos y análisis de conformación de CD y RMNarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Lozano, José ManuelEspejo, FabiolaOcampo, MarisolSalazar, Luz MaryTovar, DianaBarrera, NubiaGuzmán, FannyPatarroyo, Manuel Elkin10336/28112oai:repository.urosario.edu.co:10336/281122022-05-02 07:37:21.959605https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis |
dc.title.TranslatedTitle.spa.fl_str_mv |
Mapeo de la anatomía de un epítopo de Plasmodium falciparum MSP-1 usando anticuerpos mono y policlonales inducidos por pseudopéptidos y análisis de conformación de CD y RMN |
title |
Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis |
spellingShingle |
Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis Molecular mimicry Merozoite surface protein-1 (MSP-1) Pseudopeptide Topology Stereochemistry |
title_short |
Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis |
title_full |
Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis |
title_fullStr |
Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis |
title_full_unstemmed |
Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis |
title_sort |
Mapping the anatomy of a Plasmodium falciparum MSP-1 epitope using pseudopeptide-induced mono- and polyclonal antibodies and CD and NMR conformation analysis |
dc.subject.keyword.spa.fl_str_mv |
Molecular mimicry Merozoite surface protein-1 (MSP-1) Pseudopeptide Topology Stereochemistry |
topic |
Molecular mimicry Merozoite surface protein-1 (MSP-1) Pseudopeptide Topology Stereochemistry |
description |
Antigen structure modulation represents an approach towards designing subunit malaria vaccines. A specific epitope's ? carbon stereochemistry, as well as its backbone topochemistry, was assessed for obtaining novel malarial immunogens. A variety of MSP-138–61 Plasmodium falciparum epitope pseudopeptides derived were synthesised, based on solid-phase pseudopeptide chemistry strategies; these included all-l, all-d, partially-d substituted, all-?-[NH-CO]-Retro, all-?-[NH-CO]-Retro-inverso, and ?-[CH2NH] reduced amide surrogates. We demonstrate that specific recombinant MSP-134–469 fragment binding to red blood cells (RBCs) is specifically inhibited by non-modified MSP-142–61, as well as by its V52-L53, M51-V52 reduced amide surrogates and partial-d substitutions in K48 and E49. In vivo tests revealed that reduced amide pseudopeptide-immunised Aotus monkeys induced neutralising antibodies specifically recognising the MSP-1 N-terminus region. These findings support the role of molecular conformation in malaria vaccine development. |
publishDate |
2004 |
dc.date.created.spa.fl_str_mv |
2004-10-01 |
dc.date.accessioned.none.fl_str_mv |
2020-08-19T14:45:51Z |
dc.date.available.none.fl_str_mv |
2020-08-19T14:45:51Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.jsb.2004.04.010 |
dc.identifier.issn.none.fl_str_mv |
ISSN: 1047-8477 EISSN: 1095-8657 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/28112 |
url |
https://doi.org/10.1016/j.jsb.2004.04.010 https://repository.urosario.edu.co/handle/10336/28112 |
identifier_str_mv |
ISSN: 1047-8477 EISSN: 1095-8657 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
122 |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationStartPage.none.fl_str_mv |
110 |
dc.relation.citationTitle.none.fl_str_mv |
Journal of Structural Biology |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 148 |
dc.relation.ispartof.spa.fl_str_mv |
Journal of Structural Biology, ISSN: 1047-8477;EISSN: 1095-8657, Vol.148, No.1 (2004); pp. 110-122 |
dc.relation.uri.spa.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S1047847704001030 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_16ec |
dc.rights.acceso.spa.fl_str_mv |
Restringido (Acceso a grupos específicos) |
rights_invalid_str_mv |
Restringido (Acceso a grupos específicos) http://purl.org/coar/access_right/c_16ec |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Elsevier |
dc.source.spa.fl_str_mv |
Journal of Structural Biology |
institution |
Universidad del Rosario |
dc.source.instname.none.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.none.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167620442652672 |